LOCUS       sequence-26609-e         785 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 785)
  AUTHORS   Funakoshi M, Hochstrasser M
  TITLE     Small epitope-linker modules for PCR-based C-terminal tagging in 
            Saccharomyces cerevisiae.
  JOURNAL   Yeast. 2009 Feb 25. 26(3):185-192.
  PUBMED    19243080
REFERENCE   2  (bases 1 to 785)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast. 2009
            Feb 25. 26(3):185-192."
COMMENT     Sequence Label: pFA6a-6xGLY-Strep-tagII-HIS3MX6
FEATURES             Location/Qualifiers
     source          1..785
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(74..271)
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     CDS             complement(565..579)
                     /codon_start=1
                     /product="tag for protein cleavage using Ni2+ ion (Dang et 
                     al., 2019)"
                     /label=SNAC tag
                     /translation="GSHHW"
     gap             647..657
                     /estimated_length=11
     gap             668..684
                     /estimated_length=17
ORIGIN
        1 ctagtgganc tgatatcatc gatgaattcg agctcgttta aactggatgg cggcgttagt
       61 atcgaatcga cagcagtata gcgaccagca ttcacatacg attgacgcat gatattactt
      121 tctgcgcact taacttcgca tctgggcaga tgatgtcgag gcgaaaaaaa atataaatca
      181 cgctaacatt tgattaaaat agaacaacta caatataaaa aaactataca aatgacaagt
      241 tcttgaaaac aagaatcttt ttattgtcag tacttcacat caaaacacct ttggttgagg
      301 gaacgtcatt ggtgccattg ctagaaatag cttctcttat ggcaacagcc aaagccttga
      361 acgcactctc acttctgtgg tgatcgttga aacctctcag acaatcaaca tgcaaagtaa
      421 ttctggccgc ctccgcgaaa ctttccaaaa agtgtggaat catttcagtg gataaatcac
      481 caatcatctc tctcttcaat ccaaggtcga ttacagcaaa tggtctacta gataaatcga
      541 ctacggcacg tgatagcgcc tcatccaatg gtgcgaaccc agtaccgaat ctttttacac
      601 cacggacagc acccattgct tctttgaacg cttgccctaa tgcgatnnnn nnnnnnntnc
      661 nnnnnntnnn nnnnnnnnnn nnnngtgcag gtcaccnant acattcaaca ataagagacc
      721 aacccagant gttttgncaa cgcanggatc atatgatcca aaaagccaac accntgtgtg
      781 aatat
//
