LOCUS       sequence-266106-        8788 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  E. coli mfd gene cloned into pSKB2 with an N-terminal, ppx-cleavable
            His6 tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8788)
  AUTHORS   Deaconescu AM, Darst SA
  TITLE     Crystallization and preliminary structure determination of 
            Escherichia coli Mfd, the transcription-repair coupling factor.
  JOURNAL   Acta Crystallogr Sect F Struct Biol Cryst Commun. 2005 Dec 1;61(Pt 
            12):1062-4. doi: 10.1107/S1744309105035876. Epub 2005 Nov 24.
  PUBMED    16511235
REFERENCE   2  (bases 1 to 8788)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Acta 
            Crystallogr Sect F Struct Biol Cryst Commun."; date: "2005-12-1"; 
            volume: "61(Pt 12):1062-4. doi"; pages: " 10.1107/S1744309105035876.
            Epub 2005 Nov 24"
COMMENT     Sequence Label: pAD6
FEATURES             Location/Qualifiers
     source          1..8788
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     RBS             1..23
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             42..59
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             69..92
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             3595..3612
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(3665..3683)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      3679..3726
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      3763..4218
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3850..3869)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4060..4081
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(4310..5125)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(4310..5125)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     5033..5052
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     5117..5136
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      5247..5835
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5736..5755
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5989..6006
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    6021..6160
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(6086..6105)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6246..6268
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(6262..6453)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     CDS             complement(7262..8344)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     8306..8325
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(8345..8422)
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     8628..8647
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     8731..8750
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        8731..8749
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    8750..8774
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 tttgtttaac tttaagaagg agatatacca tgggcagcag ccatcatcat catcatcaca
       61 gcagcggcct ggaagttctg ttccaggggc cccatatggc tagcatgcct gaacaatatc
      121 gttatacgct gcccgtcaaa gcgggtgagc agcgtctgct gggcgagtta accggcgcag
      181 cctgtgcaac gctggtagcg gaaattgccg aacgtcacgc cggtccggtg gtactcattg
      241 caccagatat gcaaaatgct ctgcgtttgc atgatgaaat cagccagttt accgatcaaa
      301 tggtgatgaa tctggcggac tgggaaactc ttccctacga cagtttttcg cctcatcagg
      361 acattatctc ctcgcgcctt tccacccttt accagctacc gacgatgcag cgtggcgtac
      421 tgattgttcc ggtgaatacg cttatgcagc gcgtttgccc acacagtttt ctccacggtc
      481 atgcgctggt gatgaaaaaa ggtcagcgcc tgtcacgaga tgcattacga acccaactgg
      541 acagcgccgg ttatcgccat gttgaccagg tgatggagca cggcgaatac gccacgcgcg
      601 gcgcgttgct ggatctcttc ccgatgggga gtgagctgcc ttatcgtctt gatttctttg
      661 atgatgaaat cgacagcctg cgggtgtttg acgtcgacag ccagcgcacg ctggaggaag
      721 tagaagcgat caatctgctg cccgcgcacg aatttccgac cgataaagcg gcaattgaac
      781 tgttccgcag ccagtggcgc gataccttcg aagtgaagcg cgatccagaa catatttacc
      841 agcaagtgag taaaggcaca ttacctgccg ggatcgagta ctggcagcca ttgttcttca
      901 gcgaaccact gccgccgctg ttcagttatt tccctgccaa taccttgctc gtgaatactg
      961 gcgatctgga aaccagtgcc gaacgtttcc aggctgacac gctggcgcgt tttgagaatc
     1021 gcggcgtcga tccgatgcgc ccgctgttgc caccacaatc gctctggctg cgggtggacg
     1081 agctcttctc agagctgaaa aactggcccc gggtgcagct aaaaactgaa catttaccga
     1141 caaaagccgc gaatgccaat ttaggtttcc agaaactgcc agacctggcc gttcaggcac
     1201 aacaaaaagc gccgctggat gcgctgcgta agttcctcga gactttcgac ggtccggtgg
     1261 tgttctcggt agaaagtgaa ggtcgccgtg aagcgctggg tgaactgctc gcacgaatta
     1321 aaattgctcc gcaacgcatt atgcgtcttg atgaagccag cgaccgtggg cgttatctga
     1381 tgattggcgc tgccgaacat ggttttgtcg atacggtgcg taatctggcg ctgatttgcg
     1441 aaagcgatct gctcggtgaa cgcgttgccc gtcgtcgtca ggattctcgc cgcaccatca
     1501 accccgatac actgatccgt aaccttgcgg aactgcatat tggtcagccg gtggtccatc
     1561 tggagcacgg cgtcggtcgt tatgccggaa tgaccacgct ggaagcgggt ggcattactg
     1621 gcgagtatct gatgctcacc tatgccaacg acgccaaact gtatgttccg gtgtcgtcac
     1681 tgcatctgat tagccgttac gcaggtggcg cggaagaaaa cgccccgctg cataaacttg
     1741 gcggcgatgc gtggtcacgc gcgcggcaga aagcggcgga aaaagtgcgt gatgtggcgg
     1801 cggaattgct ggatatctac gcgcaacgcg ccgccaaaga gggcttcgcg tttaaacacg
     1861 atcgtgagca gtatcagttg ttctgcgaca gcttcccgtt tgaaaccacg ccggatcagg
     1921 cgcaggccat taatgcggta cttagcgaca tgtgtcagcc gctggcaatg gatcgtctgg
     1981 tgtgcggcga tgttggcttt ggtaaaacag aagtggcgat gcgcgcagct ttcctggcag
     2041 tagataacca caagcaggtg gcggtgctgg tgcctaccac ccttctcgcg cagcagcatt
     2101 acgacaactt ccgcgaccgt ttcgccaact ggccggtacg tatcgaaatg atctcccgtt
     2161 tccgcagcgc caaagagcag acgcaaatcc ttgcggaagt ggcggaaggg aaaatcgata
     2221 ttctgatcgg tacgcacaaa ctgctgcaaa gtgacgtcaa gtttaaagat ttaggcctgc
     2281 tgattgtcga tgaagaacac cgcttcgggg tgcgtcataa agagcgcatt aaagcgatgc
     2341 gcgcgaacgt ggatattctg acgcttactg caacgccgat cccacgtacg ctgaatatgg
     2401 caatgagcgg aatgcgtgac ctgtcgatta tcgccacgcc gcccgcccgt cgtctggcag
     2461 ttaaaacctt tgtccgtgag tatgacagca tggtggtccg ggaggcgatc ctgcgtgaaa
     2521 ttttgcgcgg aggacaggtt tattatctct acaatgatgt ggaaaacatt cagaaagccg
     2581 ccgaacggct ggcagaactg gtgccagaag cgcggatcgc catcggtcac gggcagatgc
     2641 gcgagcgcga actggaacgg gtgatgaatg atttccatca tcaacgtttc aacgtgctgg
     2701 tttgtacaac cattatcgaa accgggatcg acatcccgac agccaacact attatcattg
     2761 aacgcgcgga tcacttcggt ctggcgcagc tgcaccagtt acgcggtcgc gtcggacgtt
     2821 cgcatcatca ggcatatgca tggttgctga caccgcatcc aaaagcgatg actaccgatg
     2881 cacaaaaacg tcttgaagca attgcctcgc tggaagatct cggggcaggt tttgcgctgg
     2941 caacgcacga tctggagatt cgcggcgcgg gtgaactgct tggcgaagaa caaagcggct
     3001 caatggaaac catcggtttc tcgctgtata tggagttgct ggaaaacgcc gtcgatgcac
     3061 tgaaagccgg acgcgagccg tcgctggaag atctcaccag ccagcaaaca gaagtcgagc
     3121 tgcggatgcc gtcgctattg ccagatgatt tcatccctga cgtgaacacg cgtctgtcgt
     3181 tctacaaacg tattgccagc gccaaaacgg aaaacgaact ggaagagatc aaagtcgagc
     3241 ttatcgatcg cttcggcctg ctgccagatc cggcgcgtac cctgctggat attgcccgtc
     3301 tgcgccagca agcgcagaaa ctggggatca ggaagctgga aggtaatgag aaaggcgggg
     3361 tgatcgaatt tgccgagaag aatcacgtta atccggcctg gttgattggt ttgctgcaaa
     3421 aacagccgca gcattaccgc cttgatggtc cgacgcgcct gaaatttatt caggatttga
     3481 gtgagcggaa aacgcgtatc gaatgggtac gccagtttat gcgtgaactg gaagagaacg
     3541 cgatcgctta atgagaattc gagctccgtc gacaagcttg cggccgcact cgagcaccac
     3601 caccaccacc actgagatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct
     3661 gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt
     3721 tttttgctga aaggaggaac tatatccgga ttggcgaatg ggacgcgccc tgtagcggcg
     3781 cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc
     3841 tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc
     3901 gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg
     3961 accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg
     4021 tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg
     4081 gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt
     4141 cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa
     4201 tattaacgct tacaatttag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg
     4261 tttatttttc taaatacatt caaatatgta tccgctcatg aattaattct tagaaaaact
     4321 catcgagcat caaatgaaac tgcaatttat tcatatcagg attatcaata ccatattttt
     4381 gaaaaagccg tttctgtaat gaaggagaaa actcaccgag gcagttccat aggatggcaa
     4441 gatcctggta tcggtctgcg attccgactc gtccaacatc aatacaacct attaatttcc
     4501 cctcgtcaaa aataaggtta tcaagtgaga aatcaccatg agtgacgact gaatccggtg
     4561 agaatggcaa aagtttatgc atttctttcc agacttgttc aacaggccag ccattacgct
     4621 cgtcatcaaa atcactcgca tcaaccaaac cgttattcat tcgtgattgc gcctgagcga
     4681 gacgaaatac gcgatcgctg ttaaaaggac aattacaaac aggaatcgaa tgcaaccggc
     4741 gcaggaacac tgccagcgca tcaacaatat tttcacctga atcaggatat tcttctaata
     4801 cctggaatgc tgttttcccg gggatcgcag tggtgagtaa ccatgcatca tcaggagtac
     4861 ggataaaatg cttgatggtc ggaagaggca taaattccgt cagccagttt agtctgacca
     4921 tctcatctgt aacatcattg gcaacgctac ctttgccatg tttcagaaac aactctggcg
     4981 catcgggctt cccatacaat cgatagattg tcgcacctga ttgcccgaca ttatcgcgag
     5041 cccatttata cccatataaa tcagcatcca tgttggaatt taatcgcggc ctagagcaag
     5101 acgtttcccg ttgaatatgg ctcataacac cccttgtatt actgtttatg taagcagaca
     5161 gttttattgt tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac
     5221 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
     5281 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
     5341 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta
     5401 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
     5461 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
     5521 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
     5581 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
     5641 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
     5701 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
     5761 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
     5821 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
     5881 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc
     5941 gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg
     6001 agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt
     6061 tcacaccgca atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt
     6121 atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc
     6181 cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac
     6241 cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca
     6301 gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc
     6361 cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc
     6421 catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct
     6481 gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga
     6541 tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg
     6601 gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg
     6661 tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg
     6721 cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt
     6781 tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga
     6841 ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag
     6901 cacgatcatg cgcacccgtg gggccgccat gccggcgata atggcctgct tctcgccgaa
     6961 acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac
     7021 cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac
     7081 ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc
     7141 ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa
     7201 gggcatcggt cgagatcccg gtgcctaatg agtgagctaa cttacattaa ttgcgttgcg
     7261 ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca
     7321 acgcgcgggg agaggcggtt tgcgtattgg gcgccagggt ggtttttctt ttcaccagtg
     7381 agacgggcaa cagctgattg cccttcaccg cctggccctg agagagttgc agcaagcggt
     7441 ccacgctggt ttgccccagc aggcgaaaat cctgtttgat ggtggttaac ggcgggatat
     7501 aacatgagct gtcttcggta tcgtcgtatc ccactaccga gatatccgca ccaacgcgca
     7561 gcccggactc ggtaatggcg cgcattgcgc ccagcgccat ctgatcgttg gcaaccagca
     7621 tcgcagtggg aacgatgccc tcattcagca tttgcatggt ttgttgaaaa ccggacatgg
     7681 cactccagtc gccttcccgt tccgctatcg gctgaatttg attgcgagtg agatatttat
     7741 gccagccagc cagacgcaga cgcgccgaga cagaacttaa tgggcccgct aacagcgcga
     7801 tttgctggtg acccaatgcg accagatgct ccacgcccag tcgcgtaccg tcttcatggg
     7861 agaaaataat actgttgatg ggtgtctggt cagagacatc aagaaataac gccggaacat
     7921 tagtgcaggc agcttccaca gcaatggcat cctggtcatc cagcggatag ttaatgatca
     7981 gcccactgac gcgttgcgcg agaagattgt gcaccgccgc tttacaggct tcgacgccgc
     8041 ttcgttctac catcgacacc accacgctgg cacccagttg atcggcgcga gatttaatcg
     8101 ccgcgacaat ttgcgacggc gcgtgcaggg ccagactgga ggtggcaacg ccaatcagca
     8161 acgactgttt gcccgccagt tgttgtgcca cgcggttggg aatgtaattc agctccgcca
     8221 tcgccgcttc cactttttcc cgcgttttcg cagaaacgtg gctggcctgg ttcaccacgc
     8281 gggaaacggt ctgataagag acaccggcat actctgcgac atcgtataac gttactggtt
     8341 tcacattcac caccctgaat tgactctctt ccgggcgcta tcatgccata ccgcgaaagg
     8401 ttttgcgcca ttcgatggtg tccgggatct cgacgctctc ccttatgcga ctcctgcatt
     8461 aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca
     8521 tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg
     8581 aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg
     8641 atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg
     8701 tagaggatcg agatctcgat cccgcgaaat taatacgact cactataggg gaattgtgag
     8761 cggataacaa ttcccctcta gaaataat
//
