LOCUS       sequence-266108-       10904 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  A binary plasmid for gateway 1-Entry in pTF101.1 background (attR1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10904)
  AUTHORS   Paz, M.M., Shou, H., Guo, Z., Zhang, Z., Banerjee, A., Wang, K
  TITLE     Assessment of conditions affecting Agrobacterium-mediated soybean 
            transformation using the cotyledonary node explant
  JOURNAL   Euphytica (2004) 136: 167.
REFERENCE   2  (bases 1 to 10904)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Euphytica 
            (2004) 136: 167."
COMMENT     Sequence Label: pTF101.1gw1
FEATURES             Location/Qualifiers
     source          1..10904
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        622..966
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     910..930
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             1118..1666
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     primer_bind     complement(2236..2253)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2236..2252)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2245..2267)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     misc_feature    2467..2491
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             3012..3803
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      4049..4637
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4538..4557
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4791..4808
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    4823..4963
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(4889..4908)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5049..5071
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      5307..5501
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(5567..6640)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(7069..7698)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    9029..9053
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     protein_bind    9211..9232
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9247..9252,9253..9270,9271..9277)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9285..9301
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9290..9312
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9309..9325
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9309..9325
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     protein_bind    9351..9475
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(9516..9821)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VPRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(9618..9637)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     CDS             complement(10141..10821)
                     /codon_start=1
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQAGRNLE
                     DPAY"
     primer_bind     10736..10755
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(join(10875..10881,10882..10899,10900..10904,1))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
ORIGIN
        1 agcctggggt gcctaatgcg gccgccatag tgactggata tgttgtgttt tacagtatta
       61 tgtagtctgt tttttatgca aaatctaatt taatatattg atatttatat cattttacgt
      121 ttctcgttca gcttttttga caaacttgtt tgatgagctc ggtacccggg gatcctctag
      181 agtcgacctg caggcatgca agcttgcatg cctgcaggtc aacatggtgg agcacgacac
      241 acttgtctac tccaaaaata tcaaagatac agtctcagaa gaccaaaggg caattgagac
      301 ttttcaacaa agggtaatat ccggaaacct cctcggattc cattgcccag ctatctgtca
      361 ctttattgtg aagatagtgg aaaaggaagg tggctcctac aaatgccatc attgcgataa
      421 aggaaaggcc atcgttgaag atgcctctgc cgacagtggt cccaaagatg gacccccacc
      481 cacgaggagc atcgtggaaa aagaagacgt tccaaccacg tcttcaaagc aagtggattg
      541 atgtgataac atggtggagc acgacacact tgtctactcc aaaaatatca aagatacagt
      601 ctcagaagac caaagggcaa ttgagacttt tcaacaaagg gtaatatccg gaaacctcct
      661 cggattccat tgcccagcta tctgtcactt tattgtgaag atagtggaaa aggaaggtgg
      721 ctcctacaaa tgccatcatt gcgataaagg aaaggccatc gttgaagatg cctctgccga
      781 cagtggtccc aaagatggac ccccacccac gaggagcatc gtggaaaaag aagacgttcc
      841 aaccacgtct tcaaagcaag tggattgatg tgatatctcc actgacgtaa gggatgacgc
      901 acaatcccac tatccttcgc aagacccttc ctctatataa ggaagttcat ttcatttgga
      961 gaggacctcg agaattaatt ctcaacacaa catatacaaa acaaacgaat ctcaagcaat
     1021 caagcattct acttctattg cagcaattta aatcatttct tttaaagcaa aagcaatttt
     1081 ctgaaaattt tcaccattta cgaacggggg atctaccatg agcccagaac gacgcccggc
     1141 cgacatccgc cgtgccaccg aggcggacat gccggcggtc tgcaccatcg tcaaccacta
     1201 catcgagaca agcacggtca acttccgtac cgagccgcag gaaccgcagg agtggacgga
     1261 cgacctcgtc cgtctgcggg agcgctatcc ctggctcgtc gccgaggtgg acggcgaggt
     1321 cgccggcatc gcctacgcgg gcccctggaa ggcacgcaac gcctacgact ggacggccga
     1381 gtcgaccgtg tacgtctccc cccgccacca gcggacggga ctgggctcca cgctctacac
     1441 ccacctgctg aagtccctgg aggcacaggg cttcaagagc gtggtcgctg tcatcgggct
     1501 gcccaacgac ccgagcgtgc gcatgcacga ggcgctcgga tatgcccccc gcggcatgct
     1561 gcgggcggcc ggcttcaagc acgggaactg gcatgacgtg ggtttctggc agctggactt
     1621 cagcctgccg gtaccgcccc gtccggtcct gcccgtcacc gagatctgct caacaatcta
     1681 gctagagttt gctcctatct atatgtaata aggtatgctg atatgcacta ttcaaatagg
     1741 agcattagct atgtttgtta atgtcacttt atgttatgtg ggtaagtcac ctaagacact
     1801 ccacgtacct acttgttgtc tcttacgcgg ctttaataaa tcttctgccc ttgttccata
     1861 tttactaatt atccctttct tcactaaaag aaaattgtta tcattaagta ttagtcttta
     1921 gaacatatga ggtctttaat tgggtaggtt ttacaaatta actaatataa aatgtcataa
     1981 aatccacgtg gttaaacaaa tgcagaaaat cgacgtcgtc tattggaccg acagttgcta
     2041 ttaatataat gggccaccat agtagactga caaataaatt acctgacaac atcgtttcac
     2101 aaaaaaacaa acacaaaaag ggagtgcatt ttccagggca tttttgtaat aaaaaacagt
     2161 taaaagggag tgcaatagaa atataggggt gtggaaatag tgatttgagc acgtcttgaa
     2221 gcgaattagc ttggcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt
     2281 tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga
     2341 ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat gctagagcaa
     2401 ttcggcgtta attcagtaca ttaaaaacgt ccgcaatgtg ttattaagtt gtctaagcgt
     2461 caatttgttt acaccacaat atatcctgcc accagccagc caacagctcc ccgaccggca
     2521 gctcggcaca aaatcaccac tcgatacagg cagcccatca gtccgggacg gcgtcagcgg
     2581 gagagccgtt gtaaggcggc agactttgct catgttaccg atgctattcg gaagaacggc
     2641 aactaagctg ccgggtttga aacacggatg atctcgcgga gggtagcatg ttgattgtaa
     2701 cgatgacaga gcgttgctgc ctgtgatcaa ttcgggcacg aacccagtgg acataagcct
     2761 gttcggttcg taagctgtaa tgcaagtagc gtatgcgctc acgcaactgg tccagaacct
     2821 tgaccgaacg cagcggtggt aacggcgcag tggcggtttt catggcttgt tatgactgtt
     2881 tttttggggt acagtctatg cctcgggcat ccaagcagca agcgcgttac gccgtgggtc
     2941 gatgtttgat gttatggagc agcaacgatg ttacgcagca gggcagtcgc cctaaaacaa
     3001 agttaaacat catgggggaa gcggtgatcg ccgaagtatc gactcaacta tcagaggtag
     3061 ttggcgtcat cgagcgccat ctcgaaccga cgttgctggc cgtacatttg tacggctccg
     3121 cagtggatgg cggcctgaag ccacacagtg atattgattt gctggttacg gtgaccgtaa
     3181 ggcttgatga aacaacgcgg cgagctttga tcaacgacct tttggaaact tcggcttccc
     3241 ctggagagag cgagattctc cgcgctgtag aagtcaccat tgttgtgcac gacgacatca
     3301 ttccgtggcg ttatccagct aagcgcgaac tgcaatttgg agaatggcag cgcaatgaca
     3361 ttcttgcagg tatcttcgag ccagccacga tcgacattga tctggctatc ttgctgacaa
     3421 aagcaagaga acatagcgtt gccttggtag gtccagcggc ggaggaactc tttgatccgg
     3481 ttcctgaaca ggatctattt gaggcgctaa atgaaacctt aacgctatgg aactcgccgc
     3541 ccgactgggc tggcgatgag cgaaatgtag tgcttacgtt gtcccgcatt tggtacagcg
     3601 cagtaaccgg caaaatcgcg ccgaaggatg tcgctgccga ctgggcaatg gagcgcctgc
     3661 cggcccagta tcagcccgtc atacttgaag ctagacaggc ttatcttgga caagaagaag
     3721 atcgcttggc ctcgcgcgca gatcagttgg aagaatttgt ccactacgtg aaaggcgaga
     3781 tcaccaaggt agtcggcaaa taatgtctag ctagaaattc gttcaagccg acgccgcttc
     3841 gcggcgcggc ttaactcaag cgttagatgc actaagcaca taattgctca cagccaaact
     3901 atcaggtcaa gtctgctttt attattttta agcgtgcata ataagcccta cacaaattgg
     3961 gagatatatc atgcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
     4021 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
     4081 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
     4141 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc
     4201 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
     4261 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
     4321 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
     4381 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
     4441 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
     4501 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
     4561 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
     4621 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
     4681 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta
     4741 ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag
     4801 tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta
     4861 tttcacaccg catatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc
     4921 agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac
     4981 acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt
     5041 gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag
     5101 gcagggtgcc ttgatgtggg cgccggcggt cgagtggcga cggcgcggct tgtccgcgcc
     5161 ctggtagatt gcctggccgt aggccagcca tttttgagcg gccagcggcc gcgataggcc
     5221 gacgcgaagc ggcggggcgt agggagcgca gcgaccgaag ggtaggcgct ttttgcagct
     5281 cttcggctgt gcgctggcca gacagttatg cacaggccag gcgggtttta agagttttaa
     5341 taagttttaa agagttttag gcggaaaaat cgcctttttt ctcttttata tcagtcactt
     5401 acatgtgtga ccggttccca atgtacggct ttgggttccc aatgtacggg ttccggttcc
     5461 caatgtacgg ctttgggttc ccaatgtacg tgctatccac aggaaagaga ccttttcgac
     5521 ctttttcccc tgctagggca atttgcccta gcatctgctc cgtacattag gaaccggcgg
     5581 atgcttcgcc ctcgatcagg ttgcggtagc gcatgactag gatcgggcca gcctgccccg
     5641 cctcctcctt caaatcgtac tccggcaggt catttgaccc gatcagcttg cgcacggtga
     5701 aacagaactt cttgaactct ccggcgctgc cactgcgttc gtagatcgtc ttgaacaacc
     5761 atctggcttc tgccttgcct gcggcgcggc gtgccaggcg gtagagaaaa cggccgatgc
     5821 cgggatcgat caaaaagtaa tcggggtgaa ccgtcagcac gtccgggttc ttgccttctg
     5881 tgatctcgcg gtacatccaa tcagctagct cgatctcgat gtactccggc cgcccggttt
     5941 cgctctttac gatcttgtag cggctaatca aggcttcacc ctcggatacc gtcaccaggc
     6001 ggccgttctt ggccttcttc gtacgctgca tggcaacgtg cgtggtgttt aaccgaatgc
     6061 aggtttctac caggtcgtct ttctgctttc cgccatcggc tcgccggcag aacttgagta
     6121 cgtccgcaac gtgtggacgg aacacgcggc cgggcttgtc tcccttccct tcccggtatc
     6181 ggttcatgga ttcggttaga tgggaaaccg ccatcagtac caggtcgtaa tcccacacac
     6241 tggccatgcc ggccggccct gcggaaacct ctacgtgccc gtctggaagc tcgtagcgga
     6301 tcacctcgcc agctcgtcgg tcacgcttcg acagacggaa aacggccacg tccatgatgc
     6361 tgcgactatc gcgggtgccc acgtcataga gcatcggaac gaaaaaatct ggttgctcgt
     6421 cgcccttggg cggcttccta atcgacggcg caccggctgc cggcggttgc cgggattctt
     6481 tgcggattcg atcagcggcc gcttgccacg attcaccggg gcgtgcttct gcctcgatgc
     6541 gttgccgctg ggcggcctgc gcggccttca acttctccac caggtcatca cccagcgccg
     6601 cgccgatttg taccgggccg gatggtttgc gaccgctcac gccgattcct cgggcttggg
     6661 ggttccagtg ccattgcagg gccggcagac aacccagccg cttacgcctg gccaaccgcc
     6721 cgttcctcca cacatggggc attccacggc gtcggtgcct ggttgttctt gattttccat
     6781 gccgcctcct ttagccgcta aaattcatct actcatttat tcatttgctc atttactctg
     6841 gtagctgcgc gatgtattca gatagcagct cggtaatggt cttgccttgg cgtaccgcgt
     6901 acatcttcag cttggtgtga tcctccgccg gcaactgaaa gttgacccgc ttcatggctg
     6961 gcgtgtctgc caggctggcc aacgttgcag ccttgctgct gcgtgcgctc ggacggccgg
     7021 cacttagcgt gtttgtgctt ttgctcattt tctctttacc tcattaactc aaatgagttt
     7081 tgatttaatt tcagcggcca gcgcctggac ctcgcgggca gcgtcgccct cgggttctga
     7141 ttcaagaacg gttgtgccgg cggcggcagt gcctgggtag ctcacgcgct gcgtgatacg
     7201 ggactcaaga atgggcagct cgtacccggc cagcgcctcg gcaacctcac cgccgatgcg
     7261 cgtgcctttg atcgcccgcg acacgacaaa ggccgcttgt agccttccat ccgtgacctc
     7321 aatgcgctgc ttaaccagct ccaccaggtc ggcggtggcc catatgtcgt aagggcttgg
     7381 ctgcaccgga atcagcacga agtcggctgc cttgatcgcg gacacagcca agtccgccgc
     7441 ctggggcgct ccgtcgatca ctacgaagtc gcgccggccg atggccttca cgtcgcggtc
     7501 aatcgtcggg cggtcgatgc cgacaacggt tagcggttga tcttcccgca cggccgccca
     7561 atcgcgggca ctgccctggg gatcggaatc gactaacaga acatcggccc cggcgagttg
     7621 cagggcgcgg gctagatggg ttgcgatggt cgtcttgcct gacccgcctt tctggttaag
     7681 tacagcgata accttcatgc gttccccttg cgtatttgtt tatttactca tcgcatcata
     7741 tacgcagcga ccgcatgacg caagctgttt tactcaaata cacatcacct ttttagacgg
     7801 cggcgctcgg tttcttcagc ggccaagctg gccggccagg ccgccagctt ggcatcagac
     7861 aaaccggcca ggatttcatg cagccgcacg gttgagacgt gcgcgggcgg ctcgaacacg
     7921 tacccggccg cgatcatctc cgcctcgatc tcttcggtaa tgaaaaacgg ttcgtcctgg
     7981 ccgtcctggt gcggtttcat gcttgttcct cttggcgttc attctcggcg gccgccaggg
     8041 cgtcggcctc ggtcaatgcg tcctcacgga aggcaccgcg ccgcctggcc tcggtgggcg
     8101 tcacttcctc gctgcgctca agtgcgcggt acagggtcga gcgatgcacg ccaagcagtg
     8161 cagccgcctc tttcacggtg cggccttcct ggtcgatcag ctcgcgggcg tgcgcgatct
     8221 gtgccggggt gagggtaggg cgggggccaa acttcacgcc tcgggccttg gcggcctcgc
     8281 gcccgctccg ggtgcggtcg atgattaggg aacgctcgaa ctcggcaatg ccggcgaaca
     8341 cggtcaacac catgcggccg gccggcgtgg tggtgtcggc ccacggctct gccaggctac
     8401 gcaggcccgc gccggcctcc tggatgcgct cggcaatgtc cagtaggtcg cgggtgctgc
     8461 gggccaggcg gtctagcctg gtcactgtca caacgtcgcc agggcgtagg tggtcaagca
     8521 tcctggccag ctccgggcgg tcgcgcctgg tgccggtgat cttctcggaa aacagcttgg
     8581 tgcagccggc cgcgtgcagt tcggcccgtt ggttggtcaa gtcctggtcg tcggtgctga
     8641 cgcgggcata gcccagcagg ccagcggcgg cgctcttgtt catggcgtaa tgtctccggt
     8701 tctagtcgca agtattctac tttatgcgac taaaacacgc gacaagaaaa cgccaggaaa
     8761 agggcagggc ggcagcctgt cgcgtaactt aggacttgtg cgacatgtcg ttttcagaag
     8821 acggctgcac tgaacgtcag aagccgactg cactatagca gcggaggggt tggatcaaag
     8881 tactttaaag tactttaaag tactttaaag tactttgatc ccgaggggaa ccctgtggtt
     8941 ggcatgcaca tacaaatgga cgaacggata aaccttttca cgccctttta aatatccgat
     9001 tattctaata aacgctcttt tctcttaggt ttacccgcca atatatcctg tcaaacactg
     9061 atagtttaaa ctgaaggcgg gaaacgacaa tctgatccaa gctcaagctc caatacgcaa
     9121 accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga
     9181 ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc attaggcacc
     9241 ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga gcggataaca
     9301 atttcacaca ggaaacagct atgacatgat tacgaattcg agctcatcga accactttgt
     9361 acaagaaagc tgaacgagaa acgtaaaatg atataaatat caatatatta aattagattt
     9421 tgcataaaaa acagactaca taatactgta aaacacaaca tatccagtca ctatggtcga
     9481 cctgcagact ggctgtgtat aagggagcct gacatttata ttccccagaa catcaggtta
     9541 atggcgtttt tgatgtcatt ttcgcggtgg ctgagatcag ccacttcttc cccgataacg
     9601 gagaccggca cactggccat atcggtggtc atcatgcgcc agctttcatc cccgatatgc
     9661 accaccgggt aaagttcacg ggggacttta tctgacagca gacgtgcact ggccaggggg
     9721 atcaccatcc gtcgcccggg cgtgtcaata atatcactct gtacatccac aaacagacga
     9781 taacggctct ctcttttata ggtgtaaacc ttaaactgca tttcaccagc ccctgttctc
     9841 gtcagcaaaa gagccgttca tttcaataaa ccgggcgacc tcagccatcc cttcctgatt
     9901 ttccgctttc cagcgttcgg cacgcagacg acgggcttca ttctgcatgg ttgtgcttac
     9961 cagaccggag atattgacat catatatgcc ttgagcaact gatagctgtc gctgtcaact
    10021 gtcactgtaa tacgctgctt catagcatac ctctttttga catacttcgg gtatacatat
    10081 cagtatatat tcttataccg caaaaatcag cgcgcaaata cgcatactgt tatctggctt
    10141 ttagtaagcc ggatcctcta gattacgccc cgcctgccac tcatcgcagt actgttgtaa
    10201 ttcattaagc attctgccga catggaagcc atcacaaacg gcatgatgaa cctgaatcgc
    10261 cagcggcatc agcaccttgt cgccttgcgt ataatatttg cccatggtga aaacgggggc
    10321 gaagaagttg tccatattgg ccacgtttaa atcaaaactg gtgaaactca cccagggatt
    10381 ggctgagacg aaaaacatat tctcaataaa ccctttaggg aaataggcca ggttttcacc
    10441 gtaacacgcc acatcttgcg aatatatgtg tagaaactgc cggaaatcgt cgtggtattc
    10501 actccagagc gatgaaaacg tttcagtttg ctcatggaaa acggtgtaac aagggtgaac
    10561 actatcccat atcaccagct caccgtcttt cattgccata cggaattccg gatgagcatt
    10621 catcaggcgg gcaagaatgt gaataaaggc cggataaaac ttgtgcttat ttttctttac
    10681 ggtctttaaa aaggccgtaa tatccagctg aacggtctgg ttataggtac attgagcaac
    10741 tgactgaaat gcctcaaaat gttctttacg atgccattgg gatatatcaa cggtggtata
    10801 tccagtgatt tttttctcca ttttagcttc cttagctcct gaaaatctcg acggatccta
    10861 actcaaaatc cacacattat acgagccgga agcataaagt gtaa
//
