LOCUS       sequence-266115-        3385 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  BglFusion compatible IPTG inducible integration vector for 
            Streptococcus pneumoniae.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3385)
  AUTHORS   Keller LE, Rueff AS, Kurushima J, Veening JW
  TITLE     Three New Integration Vectors and Fluorescent Proteins for Use in 
            the Opportunistic Human Pathogen Streptococcus pneumoniae.
  JOURNAL   Genes (Basel). 2019 May 22;10(5). pii: genes10050394. doi: 
            10.3390/genes10050394.
  PUBMED    31121970
REFERENCE   2  (bases 1 to 3385)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genes 
            (Basel)."; date: "2019-05-22"; volume: "10(5). pii: genes10050394. 
            doi"; pages: " 10.3390/genes10050394"
COMMENT     Sequence Label: pPEPY-Plac
FEATURES             Location/Qualifiers
     source          1..3385
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    342..361
                     /label=lac operator (symmetric)
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG). The 
                     symmetric lac operator was optimized for tight binding of 
                     lac repressor."
     rep_origin      complement(1255..1843)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1335..1354)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(2473..3267)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKHEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
ORIGIN
        1 tagggtggtc tccgatcgtg tactgcagaa aactagtcgc aaaaaacccc gcttcggcgg
       61 ggttttttcg ctctagacgg tgatcaacac gctagcgcgt tgatgcaatt tctatgcgca
      121 cccgttctcg gagcactgtc cgaccgcttt ggccgccgcc cagtcctgct cgcttcgcta
      181 cttggagcca ctatcgacta cgcgatcatg gcgaccacac ccgtcctgtg gatctatcga
      241 tgcatgcagc cggtaccact agtaattgtg aggcggataa caattctcac tcattctaca
      301 gtttattctt gacattgcac tgtccccctg gtataataac taattgtgag cgctcacaat
      361 tgaattcatg agatctaaat aaataaggag gaaaattaat gccggatccc tccagtaact
      421 cgagaaagtg taagcaattc tgcagtacta ggacgccgcc aagccagctt aaacccagct
      481 caatgagctg ggttttttgt ttgttaaaaa tgaagaagaa actgtgaagc gtatttatag
      541 caaagcactc aaaagtttac cttatgggtg cttttttcgt gcttttttga aaagacaaaa
      601 aaaagaacct tgccaagcaa gattcttttg atagcgcagt tgagcagtgt ctttagtaaa
      661 tttagggact tgttttgtca tagttttgat acctgtattc gttgtttcat gagcatttga
      721 cgtttctttt catgtaggca tttcaacaat tagaatgata tttttccgag gatgaactta
      781 tatattgtga ctttttagag ttatgatgcc tcttgatttg actatcgcta tcaatttcat
      841 ccacttttac agtttcatgg ttttatgaac cgaaacatct aggtacaggt tttagcatca
      901 attgattgtt tttgagccat atgaagttga aacgtggaga tacttttctt gtgataacgc
      961 cctattcaaa gaggaataac cgtttttgga gagagttcag tactatgtgg cgctaaatac
     1021 tatatgtaaa acatatatta aacaagattt aactaaggag aattgtaaaa acaaaaatga
     1081 gaatttcata taaagtttat tctaggagta tgtatcttga ataaaaattg ttatttaagt
     1141 tctgggggtt atgatgaatg tttttatgcg gccgcgggat aacgcaggaa agaacatgtg
     1201 agcaaaaggc cagcaaaagg ccgggaaccg taaaaaggcc gcgttgctgg cgtttttcca
     1261 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
     1321 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
     1381 tgttccgacc ctgccgctta ctggatacct gtccgccttt ctcccttcgg gaagcgtggc
     1441 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
     1501 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
     1561 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
     1621 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
     1681 cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
     1741 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
     1801 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
     1861 ttctacgggg tggtaccaaa ccttactaaa gtatataatt taggcagtca attctgtata
     1921 ataatgtttg tactatcgtc gagtttttgc ttgattgcct aacgagataa attttagaag
     1981 agttttcggt gtgatttgac aagtttctga ctggaaggat agttacacta gtattgttta
     2041 ttaaaatgag cagatagaga aaatttggaa tctatttgct tattatcaat cgttttttta
     2101 gcttatttgt cttagattgg tatagaactg agctatggat taatcttctt ctatcttatc
     2161 ctgttcctaa atatgttggt agaaaaatga agcagttgtt acgagtgtct gtattggaaa
     2221 aaaattttgt ctatacaatt ctttaaatga tatgaagact aggaatgcca aaacactgac
     2281 atatttcgtt taaagcctat ccagtctctt caattgtatt tgtagaaatg ctatcagtaa
     2341 tatattcgcg aacactatga tttacaacag tgtggtcgta catctttgta actttattat
     2401 acagcttcta gcaaaaaact ggacggagtc ccgtccagaa aagtaagcac ttgttattgc
     2461 aataaaatta gcctaaaaca attcatccag taaaatataa tattttattt tctcccaatc
     2521 aggcttgatc cccagtaagt caaaaaatag ctcgacatac tgttcttccc cgatatcctc
     2581 cctgatcgac cggacgcaga aggcaatgtc ataccacttg tccgccctgc cgcttctccc
     2641 aagatcaata aagccactta ctttgccatc tttcacaaag atgttgctgt ctcccaggtc
     2701 gccgtgggaa aagacaagtt cctcttcgtg cttttccgtc tttaaaaaat catacagctc
     2761 gcgcggatct ttaaatggag tgtcttcttc ccagttttcg caatccacat cggccagatc
     2821 gttattcagt aagtaatcca attcggctaa gcggctgtct aagctattcg tatagggaca
     2881 atccgatatg tcgatggagt gaaagagcct gatgcactcc gcatacagct cgataatctt
     2941 ttcagggctt tgttcatctt catactcttc cgagcaaagg acgccatcgg cctcactcat
     3001 gagcagattg ctccagccat catgccgttc aaagtgcagg acctttggaa caggcagctt
     3061 tccttccagc catagcatca tgtccttttc ccgttccaca tcataggtgg tccctttata
     3121 ccggctgtcc gtcattttta aatataggtt ttcattttct cccaccagct tatatacctt
     3181 agcaggagac attccttccg tatcttttac gcagcggtat ttttcgatca gttttttcaa
     3241 ttccggtgat attctcattt tagccattaa ttttcctcct tatttattta gatccttgca
     3301 tgtatagtta ttataccagg gggacagtgc aatgtcaagc tgaaatcttc gagcagaaga
     3361 accttgccaa gcaagattct tttga
//
