LOCUS       sequence-266116-        9002 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian acoustic reporter gene 1 cassette 3 on the piggyBac 
            trasposon plasmid..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9002)
  AUTHORS   Farhadi A, Ho GH, Sawyer DP, Bourdeau RW, Shapiro MG
  TITLE     Ultrasound imaging of gene expression in mammalian cells.
  JOURNAL   Science. 2019 Sep 27;365(6460):1469-1475. doi: 
            10.1126/science.aax4804.
  PUBMED    31604277
REFERENCE   2  (bases 1 to 9002)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1126/science"; 
            journalName: "Science"; date: "2019-09-27- 27"; volume: "365"; 
            issue: "6460"; pages: "1469-1475"
COMMENT     Sequence Label: mARG1 cassette 3
FEATURES             Location/Qualifiers
     source          1..9002
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1..17
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     repeat_region   147..209
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     polyA_signal    664..745
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        complement(1635..1653)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(1636..1653)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     promoter        1726..2075
                     /label=TRE3GV promoter
                     /note="3rd-generation Tet-responsive promoter that can be 
                     activated by binding of Tet-On(R) 3G, optimized for 
                     retroviral and lentiviral vectors"
     protein_bind    1734..1752
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1770..1788
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1806..1824
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1842..1860
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1878..1896
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1914..1932
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1950..1968
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     regulatory      2089..2098
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2413..2469
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     regulatory      2596..2605
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      2600..2609
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3253..3309
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             3592..3648
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             4474..4530
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     polyA_signal    4845..4966
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     4936..4955
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     repeat_region   5729..5763
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(6254..6270)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6254..6270)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6267..6289)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6278..6294
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6302..6308,6309..6326,6327..6332))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6347..6368
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6485..6502)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6656..7244)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6736..6755)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7415..8206,8207..8275))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8038..8057
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8276..8380)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(8406..8861)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8543..8564)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     8755..8774
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     join(8988..9002,1..8)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     join(9002,1..17)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
ORIGIN
        1 gtaaaacgac ggccagtgag cgcgcctcgt tcattcacgt ttttgaaccc gtggaggacg
       61 ggcagactcg cggtgcaaat gtgttttaca gcgtgatgga gcagatgaag atgctcgaca
      121 cgctgcagaa cacgcagcta gattaaccct agaaagataa tcatattgtg acgtacgtta
      181 aagataatca tgtgtaaaat tgacgcatgt gttttatcgg tctgtatatc gaggtttatt
      241 tattaatttg aatagatatt aagttttatt atatttacac ttacatacta ataataaatt
      301 caacaaacaa tttatttatg tttatttatt tattaaaaaa aacaaaaact caaaatttct
      361 tctataaagt aacaaaactt ttatgaggga cagccccccc ccaaagcccc cagggatgta
      421 attacgtccc tcccccgcta gggggcagca gcgagccgcc cggggctccg ctccggtccg
      481 gcgctccccc cgcatccccg agccggcagc gtgcggggac agcccgggca cggggaaggt
      541 ggcacgggat cgctttcctc tgaacgcttc tcgctgctct ttgagcctgc agacacctgg
      601 ggggatacgg ggaaaaggcc tccacggcca ctagttttcc ccgaaaagtg ccacctgacg
      661 tcggcagtga aaaaaatgct ttatttgtga aatttgtgat gctattgctt tatttgtaac
      721 cattataagc tgcaataaac aagttaacaa caacaattgc attcatttta tgtttcaggt
      781 tcagggggag gtgtgggagg ttttttaaag caagtaaaac ctctacaaat gtggtatggc
      841 tgattatgat cctctagaca tatgctgcag tcacttgtac agctcatcca tgcccagggt
      901 gatgccagcg gcggtccgaa attccagcag caccatgtgg tcccgcttct cgttggggtc
      961 cttgctcagc acgctctggg tgctcaggta gtggctatca ggcagcagca cggggccatc
     1021 tccgatgggg gtgttctgct ggtagtggtc ggccagctgc acgctgccat cttccacgtt
     1081 gtgccggatc ttgaagttca ctttgatgcc gtttttctgc ttcacggcca tgatgtagat
     1141 gttgtggctg ttgaagttgt actccagctt gtggcccagg atgttgccgt cctctttgaa
     1201 gtccacgccc ttcagctcga tccggttcac gagggtgtcg ccctcgaact tcacttcggc
     1261 tctggtcttg taggtgccgt cgtccttgaa gaagatggtc cgttcctgca cgtagccctc
     1321 gggcatggcg ctcttgaaga aatcgtgctg cttcatgtgg tcggggtatc tggcgaagca
     1381 ctgcacgccg tgagacagtg tggtcacgag ggtaggccaa ggcacgggca gcttgccggt
     1441 ggtgcagatg aacttcaggg tcagcttgcc atttgtggcg tcgccttcgc cctctccccg
     1501 cacagagaac ttgtggccgt tcacgtcgcc atccagttcc accaggatgg gcaccacgcc
     1561 ggtgaacagt tcctcgccct tggacaccat ggtgaagggt actggatccg agctcggtac
     1621 ctgcaggcgt accttctata gtgtcaccta aatgcgatct gacggttcac taaacgagct
     1681 ctgcttatat aggcctccca ccgtacacgc cacctcgaca tactcgagtt tactccctat
     1741 cagtgataga gaacgtatga agagtttact ccctatcagt gatagagaac gtatgcagac
     1801 tttactccct atcagtgata gagaacgtat aaggagttta ctccctatca gtgatagaga
     1861 acgtatgacc agtttactcc ctatcagtga tagagaacgt atctacagtt tactccctat
     1921 cagtgataga gaacgtatat ccagtttact ccctatcagt gatagagaac gtatgtcgag
     1981 gtaggcgtgt acggtgggcg cctataaaag cagagctcgt ttagtgaacc gtcagatcgc
     2041 ctggagcaat tccacaacac ttttgtctta tacttggtac ctatgcatgc caccatggcc
     2101 gtggaacaca acatgcagag cagcaccatc gtggacgtgc tggaaaagat cctggacaag
     2161 ggcgtcgtga tcgccgggga catcacagtg ggaatcgccg acgtggaact gctgaccatc
     2221 aagatccggc tgatcgtggc cagcgtggac aaggccaaag aaatcggcat ggattggtgg
     2281 gagaacgacc cctacctgag cagcaagggc gccaacaaca aggccctgga agaggaaaac
     2341 aagatgctgc acgagcggct gaaaacactg gaagagaaga tcgagacaaa gcgcggtgcc
     2401 ccgggatctg gcgcaacaaa ttttagtctt ttaaagcagg caggagacgt cgaggaaaac
     2461 cctggacccg tgagcgagac aaacgagaca ggcatctaca tcttcagcgc catccagaca
     2521 gacaaggatg aggaattcgg cgccgtggaa gtggaaggga ccaaggctga gacattcctg
     2581 atccggtata aggacgccgc catggtggcc gccgaagtgc ccatgaagat ctaccacccc
     2641 aaccggcaga acctgctgat gcaccagaat gccgtggccg ccatcatgga caagaacgac
     2701 accgtgatcc ccatcagctt cggcaacgtg ttcaagagca aagaggacgt gaaggtgctc
     2761 ctggaaaacc tgtaccccca gttcgagaag ctgttccccg ccatcaaggg aaagatcgaa
     2821 gtgggcctga aggtgatcgg caagaaagag tggctcgaaa agaaagtgaa cgagaacccc
     2881 gagctggaaa aagtgtccgc cagcgtgaag ggcaagagcg aggccgctgg ctactacgag
     2941 agaatccagc tgggcggcat ggcccagaag atgttcacaa gcctgcagaa agaagtgaaa
     3001 accgacgtgt tcagccccct ggaagaagcc gccgaggccg ccaaagccaa tgagcctaca
     3061 ggcgaaacaa tgctgctgaa cgccagcttc ctgatcaaca gagaggatga ggccaagttc
     3121 gacgagaaag tcaatgaggc ccacgagaac tggaaggata aggccgactt ccactacagc
     3181 ggcccctggc ccgcctacaa cttcgtgaac atccggctga aggtggaaga gaagggggca
     3241 cctggctcgg gagcgaccaa cttctcatta ctcaaacaag ccggagacgt tgaggagaat
     3301 ccaggccctg tgctgcacaa gctcgtgacc gcccccatca acctggtcgt gaagatcggc
     3361 gagaaggtgc aggaagaggc cgacaagcag ctgtacgacc tgcccaccat ccagcagaag
     3421 ctgatccagc tgcagatgat gttcgagctg ggcgagatcc ccgaggaagc cttccaggaa
     3481 aaagaggacg aactgctgat gagatacgag atcgccaagc ggcgcgagat tgagcagtgg
     3541 gaagaactga cccagaagcg gaatgaggaa agcggtgccc cgggatctgg cgcaacaaat
     3601 tttagtcttt taaagcaggc aggagacgtc gaggaaaacc ctggacccgt gggcgagctg
     3661 ctgtacctct acggcctgat ccccaccaaa gaggccgctg ctatcgagcc cttcccattc
     3721 tacaagggct tcgacggcga gcacagcctg taccctatcg ccttcgacca agtgaccgcc
     3781 gtggtgttca agctggacgc cgacacctac agcgagaaag tgatccagga aaagatggaa
     3841 caggacatga gctggctgca ggaaaaggcc ttccaccacc acgagacagt ggccgccctg
     3901 tatgaggaat tcaccatcat ccccctgaag ttctgcacca tctataaggg agaggaatcc
     3961 ctgcaggccg ccatcgagat caacaaagag aagatcgaaa actccctgac cctgctgcag
     4021 ggcaacgagg aatggaacgt gaagatctac tgcgacgaca ccgagctgaa gaagggcatc
     4081 agcgagacaa acgagagcgt gaaggccaag aagcaggaaa tcagccacct gagccccggc
     4141 agacagttct tcgagaagaa gaagattgac cagctcatcg agaaagagct ggaactgcac
     4201 aagaacaaag tgtgcgagga aatccacgac aagctgattg agctgagcct ctacgactcc
     4261 gtgaagaaga actggtccaa ggacgtgaca ggcgctgccg aacagatggc ctggaacagc
     4321 gtgttcctgc tgcccagcct gcagatcacc aagttcgtga acgagatcga ggaactccag
     4381 cagcggctgg agaacaaggg atggaagttc gaagtgaccg gcccctggcc tccctaccac
     4441 ttcagcagct ttgccggggc acctggctcg ggagcgacca acttctcatt actcaaacaa
     4501 gccggagacg ttgaggagaa tccaggccct gtgcagcccg tgtcccaggc caacggcaga
     4561 atccacctgg atcccgatca ggccgaacag ggactggccc agctcgtgat gaccgtgatc
     4621 gagctgctgc ggcagatcgt ggaacggcac gccatgagaa gagtggaagg cggcaccctg
     4681 accgacgagc agatcgagaa tctgggaatc gccctgatga acctggaaga gaagatggac
     4741 gagctgaaag aggtgttcgg actggacgcc gaggacctga acatcgacct gggccctctg
     4801 ggcagcctgc tgtgaactag ttcgataccg tcgaccgtta actaaacttg tttattgcag
     4861 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt
     4921 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggaatt
     4981 gactcaaatg atgtcaatta gtctatcaga agctcatctg gtctcccttc cgggggacaa
     5041 gacatccctg tttaatattt aaacagcagt gttcccaaac tgggttctta tatcccttgc
     5101 tctggtcaac caggttgcag ggtttcctgt cctcacagga acgaagtccc taaagaaaca
     5161 gtggcagcca ggtttagccc cggaattgac tggattcctt ttttagggcc cattggtatg
     5221 gctttttccc cgtatccccc caggtgtctg caggctcaaa gagcagcgag aagcgttcag
     5281 aggaaagcga tcccgtgcca ccttccccgt gcccgggctg tccccgcacg ctgccggctc
     5341 ggggatgcgg ggggagcgcc ggaccggagc ggagccccgg gcggctcgct gctgccccct
     5401 agcgggggag ggacgtaatt acatccctgg gggctttggg ggggggctgt ccctgatatc
     5461 tataacaaga aaatatatat ataataagtt atcacgtaag tagaacatga aataacaata
     5521 taattatcgt atgagttaaa tcttaaaagt cacgtaaaag ataatcatgc gtcattttga
     5581 ctcacgcggt cgttatagtt caaaatcagt gacacttacc gcattgacaa gcacgcctca
     5641 cgggagctcc aagcggcgac tgagatgtcc taaatgcaca gcgacggatt cgcgctattt
     5701 agaaagagag agcaatattt caagaatgca tgcgtcaatt ttacgcagac tatctttcta
     5761 gggttaatct agctgcatca ggatcatatc gtcgggtctt ttttccggct cagtcatcgc
     5821 ccaagctggc gctatctggg catcggggag gaagaagccc gtgccttttc ccgcgaggtt
     5881 gaagcggcat ggaaagagtt tgccgaggat gactgctgct gcattgacgt tgagcgaaaa
     5941 cgcacgttta ccatgatgat tcgggaaggt gtggccatgc acgcctttaa cggtgaactg
     6001 ttcgttcagg ccacctggga taccagttcg tcgcggcttt tccggacaca gttccggatg
     6061 gtcagcccga agcgcatcag caacccgaac aataccggcg acagccggaa ctgccgtgcc
     6121 ggtgtgcaga ttaatgacag cggtgcggcg ctgggatatt acgtcagcga ggacgggtat
     6181 cctggctgga tgccgcagaa atggacatgg ataccccgtg agttacccgg cgggcgcgct
     6241 tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac
     6301 acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac
     6361 tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc
     6421 tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg
     6481 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc
     6541 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt
     6601 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
     6661 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
     6721 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
     6781 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
     6841 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
     6901 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
     6961 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
     7021 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
     7081 acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
     7141 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
     7201 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
     7261 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga
     7321 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa
     7381 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac
     7441 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga
     7501 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc
     7561 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca
     7621 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta
     7681 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg
     7741 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc
     7801 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg
     7861 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt
     7921 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt
     7981 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata
     8041 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc
     8101 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac
     8161 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa
     8221 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct
     8281 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat
     8341 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc
     8401 cacctaaatt gtaagcgtta atattttgtt aaaattcgcg ttaaattttt gttaaatcag
     8461 ctcatttttt aaccaatagg ccgaaatcgg caaaatccct tataaatcaa aagaatagac
     8521 cgagataggg ttgagtgttg ttccagtttg gaacaagagt ccactattaa agaacgtgga
     8581 ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat ggcccactac gtgaaccatc
     8641 accctaatca agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg
     8701 gagcccccga tttagagctt gacggggaaa gccggcgaac gtggcgagaa aggaagggaa
     8761 gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac
     8821 caccacaccc gccgcgctta atgcgccgct acagggcgcg tcccattcgc cattcaggct
     8881 gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa
     8941 agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg
     9001 tt
//
