LOCUS       sequence-266124-       10127 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses human SMCHD1Exon1-14.47M-48 with an N-terminal 3xHA tag 
            and a C-terminal 3xFLAG tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10127)
  AUTHORS   Hiramuki Y, Tapscott SJ
  TITLE     Identification of SMCHD1 domains for nuclear localization, 
            homo-dimerization, and protein cleavage.
  JOURNAL   Skelet Muscle. 2018 Aug 2;8(1):24. doi: 10.1186/s13395-018-0172-z.
  PUBMED    30071896
REFERENCE   2  (bases 1 to 10127)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Skelet 
            Muscle."; date: "2018-08-2"; volume: "8(1):24. doi"; pages: " 
            10.1186/s13395-018-0172-z"
COMMENT     Sequence Label: pLenti-CMV-3xHA-SMCHD1-Exon1-14.47M-48-3xFLAG
FEATURES             Location/Qualifiers
     source          1..10127
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    19..135
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        158..461
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        462..665
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     616..636
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     662..686
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             751..840
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     CDS             2929..2994
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     misc_feature    3004..3592
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3057..3077)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3475..3486)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        3606..4105
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(3641..3664)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     4038..4057
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             4126..4725
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4126..4145)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     4622..4642
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             4863..5096
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5168..5289
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5205..5224)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5259..5278
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5329..5464
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5484..5503)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5485..5503)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5513..5530)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5513..5529)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5522..5544)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5671..6126
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5758..5777)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5968..5989
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6152..6256
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6257..6325,6326..7117)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6475..6494)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7288..7876
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7777..7796
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8030..8047
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8164..8185
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8200..8205,8206..8223,8224..8230)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8238..8254
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8243..8265
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8262..8278
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8262..8278
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8297..8317
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        8299..8317
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        8345..8571
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             8572..8752
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8799..8924
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9417..9650
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9835..9879
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 tcgacggtat cggttaactt ttaaaagaaa aggggggatt ggggggtaca gtgcagggga
       61 aagaatagta gacataatag caacagacat acaaactaaa gaattacaaa aacaaattac
      121 aaaattcaaa attttatcga taagcttggg agttccgcgt tacataactt acggtaaatg
      181 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc
      241 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa
      301 ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca
      361 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta
      421 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt
      481 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg
      541 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca
      601 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca
      661 gagctcgttt agtgaaccgt cagatcgcct ggagacgcca tccacgctgt tttgacctcc
      721 atagaagaca ccgactctag aggatccatg tacccatacg atgttcctga ctatgcgggc
      781 tatccctatg acgtcccgga ctatgcaggc tcctatccat atgacgttcc agattacgct
      841 gcagcggcgg acggcggcgg gcctggtggg gcctctgtgg ggactgagga ggatggcgga
      901 ggcgtcggcc acaggacggt gtacttgttt gatcggcgcg aaaaggagtc cgagctcggg
      961 gaccggcctc tgcaggtcgg ggagcgctcg gactacgcgg gatttcgcgc gtgtgtgtgt
     1021 cagacacttg gcatttcacc tgaagaaaaa tttgttatta caacaacaag taggaaagaa
     1081 attacctgtg ataattttga tgaaactgtt aaagatggag tcaccttata cctgctacag
     1141 tcggtcaatc agttactact gacagctacg aaagaacgaa ttgacttctt acctcactat
     1201 gacacactgg ttaaaagtgg catgtatgaa tattatgcca gtgaaggaca aaatcctttg
     1261 ccatttgctc ttgcggaatt aattgacaat tcattgtctg ctacttctcg taacattggg
     1321 gttagaagaa tacagatcaa attgcttttt gatgaaacac aaggaaaacc tgctgttgca
     1381 gtgatagata atggaagagg aatgacctct aaacagctta acaactgggc cgtgtatagg
     1441 ttgtcaaaat tcacaaggca aggtgacttt gaaagtgatc attcaggata tgttcgtcca
     1501 gtaccagtgc cacgcagttt aaatagtgat atttcctatt ttggtgttgg gggcaagcaa
     1561 gctgtcttct ttgttggaca atcagccaga atgataagca aacctgcaga ttcccaagat
     1621 gttcacgagc ttgtgctttc taaagaagat tttgagaaga aggagaaaaa taaagaggca
     1681 atatatagtg gatatattag aaacagaaag ccctctgatt ctgttcacat tacaaatgat
     1741 gatgaaagat ttctacatca tcttatcata gaggagaagg aaaaagatag ctttactgct
     1801 gtggttatca caggggtaca accagaacac atacagtact tgaaaaatta tttccacctt
     1861 tggacacgac agttagcgca tatttatcac tactatattc atggcccaaa aggaaatgaa
     1921 atacgaacat caaaagaagt tgaacctttc aacaatattg atattgaaat ttctatgttt
     1981 gaaaaaggga aggtacctaa gattgtcaac ctaagggaaa tacaagacga catgcagacg
     2041 ttgtatgtaa acacagcagc tgatagtttt gaattcaaag ctcatgttga aggagatggt
     2101 gtagtggaag ggattatccg ttatcatcca ttcttatatg atagagaaac ttaccctgat
     2161 gatccatgct ttccatcaaa attaaaagat gaagatgatg aagatgattg tttcatactt
     2221 gagaaagcag ctagagggaa aaggcctatt tttgaatgtt tttggaatgg acgattaata
     2281 ccatatacat cagttgaaga ctttgactgg tgtactcctc ctaagaagag agggcttgca
     2341 ccaattgaat gctacaatag gatatctggt gcattattca ctaatgacaa attccaggtc
     2401 agcacaaata aattgacgtt tatggatctt gagctaaaat tgaaagataa gaacaccctt
     2461 tttacaagga ttttaaatgg acaggaacag cgaatgaaaa ttgacagaga atttgctttg
     2521 tggctgaagg actgtcatga gaagtatgat aaacaaataa aatttacact ttttaaggga
     2581 gtaattacac gtcctgatct tccttctaaa aagcaaggtc cctgggcaac atatgcagca
     2641 atagaatggg atggaaagat atacaaagca ggacagctgg tcaagacaat caagacactt
     2701 cccctctttt atggaagcat agtaagattt tttctttatg gcgatcatga tggagaagta
     2761 tatgctacag gaggagaggt tcaaattgca atgatgactc ccatacgtaa gtgtaatgac
     2821 tcattgcgtc attcaccaaa ggttgagacg acagattgtc cagttcctcc taaaagaatg
     2881 agacgagaag ctacaagaca aaataggatt ataaccaaaa cagatgtaga ctacaaagac
     2941 catgacggtg attataaaga tcatgacatc gattacaagg atgacgatga caagtgagtc
     3001 gacaatcaac ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt
     3061 gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc
     3121 cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag
     3181 ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc
     3241 actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc
     3301 cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg
     3361 ctgttgggca ctgacaattc cgtggtgttg tcggggaagc tgacgtcctt tccatggctg
     3421 ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc
     3481 ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt
     3541 cttcgccttc gccctcagac gagtcggatc tccctttggg ccgcctcccc gcctggaatt
     3601 ctaccgggta ggggaggcgc ttttcccaag gcagtctgga gcatgcgctt tagcagcccc
     3661 gctgggcact tggcgctaca caagtggcct ctggcctcgc acacattcca catccaccgg
     3721 taggcgccaa ccggctccgt tctttggtgg ccccttcgcg ccaccttcta ctcctcccct
     3781 agtcaggaag ttcccccccg ccccgcagct cgcgtcgtgc aggacgtgac aaatggaagt
     3841 agcacgtctc actagtctcg tgcagatgga cagcaccgct gagcaatgga agcgggtagg
     3901 cctttggggc agcggccaat agcagctttg ctccttcgct ttctgggctc agaggctggg
     3961 aaggggtggg tccgggggcg ggctcagggg cgggctcagg ggcggggcgg gcgcccgaag
     4021 gtcctccgga ggcccggcat tctgcacgct tcaaaagcgc acgtctgccg cgctgttctc
     4081 ctcttcctca tctccgggcc tttcgacctg cagcccaagc ttaccatgac cgagtacaag
     4141 cccacggtgc gcctcgccac ccgcgacgac gtccccaggg ccgtacgcac cctcgccgcc
     4201 gcgttcgccg actaccccgc cacgcgccac accgtcgatc cggaccgcca catcgagcgg
     4261 gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc tcgacatcgg caaggtgtgg
     4321 gtcgcggacg acggcgccgc ggtggcggtc tggaccacgc cggagagcgt cgaagcgggg
     4381 gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga gcggttcccg gctggccgcg
     4441 cagcaacaga tggaaggcct cctggcgccg caccggccca aggagcccgc gtggttcctg
     4501 gccaccgtcg gcgtctcgcc cgaccaccag ggcaagggtc tgggcagcgc cgtcgtgctc
     4561 cccggagtgg aggcggccga gcgcgccggg gtgcccgcct tcctggagac ctccgcgccc
     4621 cgcaacctcc ccttctacga gcggctcggc ttcaccgtca ccgccgacgt cgaggtgccc
     4681 gaaggaccgc gcacctggtg catgacccgc aagcccggtg cctgacgccc gccccacgac
     4741 ccgcagcgcc cgaccgaaag gagcgcacga ccccatgcat ctcgagggcc cggtaccttt
     4801 aagaccaatg acttacaagg cagctgtaga tcttagccac tttttaaaag aaaagggggg
     4861 actggaaggg ctagctcact cccaacgaag acaagatctg ctttttgctt gtactgggtc
     4921 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct
     4981 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga
     5041 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtag
     5101 tagttcatgt catcttatta ttcagtattt ataacttgca aagaaatgaa tatcagagag
     5161 tgagaggaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa
     5221 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
     5281 tgtatcttat catgtctggc tctagctatc ccgcccctaa ctccgcccat cccgccccta
     5341 actccgccca gttccgccca ttctccgccc catggctgac taattttttt tatttatgca
     5401 gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga
     5461 ggcctaggga cgtacccaat tcgccctata gtgagtcgta ttacgcgcgc tcactggccg
     5521 tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat cgccttgcag
     5581 cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc
     5641 aacagttgcg cagcctgaat ggcgaatggg acgcgccctg tagcggcgca ttaagcgcgg
     5701 cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc
     5761 ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa
     5821 atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac
     5881 ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt
     5941 tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca
     6001 accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt
     6061 taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgctta
     6121 caatttaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta
     6181 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata
     6241 ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc
     6301 ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga
     6361 agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct
     6421 tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg
     6481 tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta
     6541 ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat
     6601 gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt
     6661 acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga
     6721 tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga
     6781 gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga
     6841 actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc
     6901 aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc
     6961 cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg
     7021 tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat
     7081 cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata
     7141 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct
     7201 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
     7261 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
     7321 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
     7381 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct
     7441 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
     7501 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
     7561 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
     7621 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
     7681 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
     7741 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
     7801 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
     7861 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
     7921 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac
     7981 cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt
     8041 gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat
     8101 tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc
     8161 aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta tgcttccggc
     8221 tcgtatgttg tgtggaattg tgagcggata acaatttcac acaggaaaca gctatgacca
     8281 tgattacgcc aagcgcgcaa ttaaccctca ctaaagggaa caaaagctgg agctgcaagc
     8341 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
     8401 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
     8461 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
     8521 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
     8581 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
     8641 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
     8701 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
     8761 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
     8821 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
     8881 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
     8941 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
     9001 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
     9061 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
     9121 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
     9181 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
     9241 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
     9301 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     9361 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     9421 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     9481 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     9541 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     9601 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     9661 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     9721 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     9781 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     9841 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     9901 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     9961 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
    10021 tttcagaccc acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa
    10081 ggtggagaga gagacagaga cagatccatt cgattagtga acggatc
//
