LOCUS       sequence-266128-        3117 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pZA2-pLlacO-oxyR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3117)
  AUTHORS   Muller IE, Rubens JR, Jun T, Graham D, Xavier R, Lu TK
  TITLE     Gene networks that compensate for crosstalk with crosstalk.
  JOURNAL   Nat Commun. 2019 Sep 6;10(1):4028. doi: 10.1038/s41467-019-12021-y.
  PUBMED    31492904
REFERENCE   2  (bases 1 to 3117)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2019-09-6"; volume: "10(1):4028. doi"; pages: " 
            10.1038/s41467-019-12021-y"
COMMENT     Sequence Label: fIM1264
FEATURES             Location/Qualifiers
     source          1..3117
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..17
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    24..40
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             92..103
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     terminator      1057..1143
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     complement(1180..1197)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1314..1859)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(1394..1413)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1971..2065)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(2096..2890)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(2207..2226)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     2817..2836
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     3069..3087
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 ttgtgagcgg ataacaattg acattgtgag cggataacaa gatactgagc acatcagcag
       61 gacgcactga ccttaattaa aagaattcat taaagaggag aaaggtacca tgaatattcg
      121 tgatcttgag tacctggtgg cattggctga acaccgccat tttcggcgtg cggcagattc
      181 ctgccacgtt agccagccga cgcttagcgg gcaaattcgt aagctggaag atgagctggg
      241 cgtgatgttg ctggagcgga ccagccgtaa agtgttgttc acccaggcgg gaatgctgct
      301 ggtggatcag gcgcgtaccg tgctgcgtga ggtgaaagtc cttaaagaga tggcaagcca
      361 gcagggcgag acgatgtccg gaccgctgca cattggtttg attcccacag ttggaccgta
      421 cctgctaccg catattatcc ctatgctgca ccagaccttt ccaaagctgg aaatgtatct
      481 gcatgaagca cagacccacc agttactggc gcaactggac agcggcaaac tcgattgcgt
      541 gatcctcgcg ctggtgaaag agagcgaagc attcattgaa gtgccgttgt ttgatgagcc
      601 aatgttgctg gctatctatg aagatcaccc gtgggcgaac cgcgaatgcg taccgatggc
      661 cgatctggca ggggaaaaac tgctgatgct ggaagatggt cactgtttgc gcgatcaggc
      721 aatgggtttc tgttttgaag ccggggcgga tgaagataca cacttccgcg cgaccagcct
      781 ggaaactctg cgcaacatgg tggcggcagg tagcgggatc actttactgc cagcgctggc
      841 tgtgccgccg gagcgcaaac gcgatggggt tgtttatctg ccgtgcatta agccggaacc
      901 acgccgcact attggcctgg tttatcgtcc tggctcaccg ctgcgcagcc gctatgagca
      961 gctggcagag gccatccgcg caagaatgga tggccatttc gataaagttt taaaacaggc
     1021 ggtttaagga tcccatggta cgcgtgctag aggcatcaaa taaaacgaaa ggctcagtcg
     1081 aaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcct gagtaggaca
     1141 aatccgccgc cctagaccta gggggatata ttccgcttcc tcgctcactg actcgctacg
     1201 ctcggtcgtt cgactgcggc gagcggaaat ggcttacgaa cggggcggag atttcctgga
     1261 agatgccagg aagatactta acagggaagt gagagggccg cggcaaagcc gtttttccat
     1321 aggctccgcc cccctgacaa gcatcacgaa atctgacgct caaatcagtg gtggcgaaac
     1381 ccgacaggac tataaagata ccaggcgttt ccccctggcg gctccctcgt gcgctctcct
     1441 gttcctgcct ttcggtttac cggtgtcatt ccgctgttat ggccgcgttt gtctcattcc
     1501 acgcctgaca ctcagttccg ggtaggcagt tcgctccaag ctggactgta tgcacgaacc
     1561 ccccgttcag tccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccgga
     1621 aagacatgca aaagcaccac tggcagcagc cactggtaat tgatttagag gagttagtct
     1681 tgaagtcatg cgccggttaa ggctaaactg aaaggacaag ttttggtgac tgcgctcctc
     1741 caagccagtt acctcggttc aaagagttgg tagctcagag aaccttcgaa aaaccgccct
     1801 gcaaggcggt tttttcgttt tcagagcaag agattacgcg cagaccaaaa cgatctcaag
     1861 aagatcatct tattaatcag ataaaatatt tctagatttc agtgcaattt atctcttcaa
     1921 atgtagcacc tgaagtcagc cccatacgat ataagttgta tagtgcttgg attctcacca
     1981 ataaaaaacg cccggcggca accgagcgtt ctgaacaaat ccagatggag ttctgaggtc
     2041 attactggat ctatcaacag gagtccaagc gagctctcga accccagagt cccgctcaga
     2101 agaactcgtc aagaaggcga tagaaggcga tgcgctgcga atcgggagcg gcgataccgt
     2161 aaagcacgag gaagcggtca gcccattcgc cgccaagctc ttcagcaata tcacgggtag
     2221 ccaacgctat gtcctgatag cggtccgcca cacccagccg gccacagtcg atgaatccag
     2281 aaaagcggcc attttccacc atgatattcg gcaagcaggc atcgccatgg gtcacgacga
     2341 gatcctcgcc gtcgggcatg cgcgccttga gcctggcgaa cagttcggct ggcgcgagcc
     2401 cctgatgctc ttcgtccaga tcatcctgat cgacaagacc ggcttccatc cgagtacgtg
     2461 ctcgctcgat gcgatgtttc gcttggtggt cgaatgggca ggtagccgga tcaagcgtat
     2521 gcagccgccg cattgcatca gccatgatgg atactttctc ggcaggagca aggtgagatg
     2581 acaggagatc ctgccccggc acttcgccca atagcagcca gtcccttccc gcttcagtga
     2641 caacgtcgag cacagctgcg caaggaacgc ccgtcgtggc cagccacgat agccgcgctg
     2701 cctcgtcctg cagttcattc agggcaccgg acaggtcggt cttgacaaaa agaaccgggc
     2761 gcccctgcgc tgacagccgg aacacggcgg catcagagca gccgattgtc tgttgtgccc
     2821 agtcatagcc gaatagcctc tccacccaag cggccggaga acctgcgtgc aatccatctt
     2881 gttcaatcat gcgaaacgat cctcatcctg tctcttgatc agatcttgat cccctgcgcc
     2941 atcagatcct tggcggcaag aaagccatcc agtttacttt gcagggcttc ccaaccttac
     3001 cagagggcgc cccagctggc aattccgacg tctaagaaac cattattatc atgacattaa
     3061 cctataaaaa taggcgtatc acgaggccct ttcgtcttca cctcgagatg ctagcaa
//
