LOCUS       sequence-266138-       11197 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  60-copy, promoterless, oriE1(ColE1) luxCDABE, aph, hok-sok, par, 
            parA, rrnB Stabilized luciferase-expressing plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11197)
  AUTHORS   Morin CE, Kaper JB
  TITLE     Use of stabilized luciferase-expressing plasmids to examine in 
            vivo-induced promoters in the Vibrio cholerae vaccine strain CVD 
            103-HgR.
  JOURNAL   FEMS Immunol Med Microbiol. 2009 Oct;57(1):69-79. doi: 
            10.1111/j.1574-695X.2009.00580.x. Epub 2009 Jul 1.
  PUBMED    19678844
REFERENCE   2  (bases 1 to 11197)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1111/j.1574-695X.2009.00580.x"; journalName: "FEMS Immunol Med 
            Microbiol"; date: "2009-10"; volume: "57"; issue: "1"; pages: 
            "69-79"
COMMENT     Sequence Label: pCM18
FEATURES             Location/Qualifiers
     source          1..11197
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(356..1150)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(467..486)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     1077..1096
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     terminator      3710..3796
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      3888..3915
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     CDS             3982..5424
                     /codon_start=1
                     /gene="luxC"
                     /product="LuxC fatty acid reductase"
                     /label=LuxC
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MTKKISFIINGQVEIFPEGDDLVQSINFGDNSVYLPILNDSHVKN
                     IIDCNGNNELRLHNIVNFLYTVGQRWKNEEYSRRRTYIRDLKKYMGYSEEMAKLEANWI
                     SMILCSKGGLYDVVENELGSRHIMDEWLPQDESYVRAFPKGKSVHLLAGNVPLSGIMSI
                     LRAILTKNQCIIKTSSTDPFTANALALSFIDVDPNHPITRSLSVIYWPHQGDTSLAKEI
                     MQHADVIVAWGGPDAINWAVEHAPSYADVIKFGSKKSLCIIDNPVDLTSAATGAAHDVC
                     FYDQRACFSAQNIYYMGNHYEEFKLALIEKLNLYAHILPNAKKDFDEKAAYSLVQKESL
                     FAGLKVEVDIHQRWMIIESNAGVEFNQPLGRCVYLHHVDNIEQILPYVQKNKTQTISIF
                     PWESSFKYRDALALKGAERIVEAGMNNIFRVGGSHDGMRPLQRLVTYISHERPSNYTAK
                     DVAVEIEQTRFLEEDKFLVFVP"
     CDS             5437..6360
                     /codon_start=1
                     /gene="luxD"
                     /product="LuxD acyltransferase"
                     /label=LuxD
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MENESKYKTIDHVICVEGNKKIHVWETLPEENSPKRKNAIIIASG
                     FARRMDHFAGLAEYLSRNGFHVIRYDSLHHVGLSSGTIDEFTMSIGKQSLLAVVDWLTT
                     RKINNFGMLASSLSARIAYASLSEINASFLITAVGVVNLRYSLERALGFDYLSLPINEL
                     PNNLDFEGHKLGAEVFARDCLDFGWEDLASTINNMMYLDIPFIAFTANNDNWVKQDEVI
                     TLLSNIRSNRCKIYSLLGSSHDLSENLVVLRNFYQSVTKAAIAMDNDHLDIDVDITEPS
                     FEHLTIATVNERRMRIEIENQAISLS"
     CDS             6409..7491
                     /codon_start=1
                     /gene="luxA"
                     /product="LuxA luciferase subunit"
                     /label=LuxA
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MKFGNFLLTYQPPQFSQTEVMKRLVKLGRISEECGFDTVWLLEHH
                     FTEFGLLGNPYVAAAYLLGATKKLNVGTAAIVLPTAHPVRQLEDVNLLDQMSKGRFRFG
                     ICRGLYNKDFRVFGTDMNNSRALAECWYGLIKNGMTEGYMEADNEHIKFHKVKVNPAAY
                     SRGGAPVYVVAESASTTEWAAQFGLPMILSWIINTNEKKAQLELYNEVAQEYGHDIHNI
                     DHCLSYITSVDHDSIKAKEICRKFLGHWYDSYVNATTIFDDSDQTRGYDFNKGQWRDFV
                     LKGHKDTNRRIDYSYEINPVGTPQECIDIIQKDIDATGISNICCGFEANGTVDEIIASM
                     KLFQSDVMPFLKEKQRSLLY"
     CDS             7506..8489
                     /codon_start=1
                     /gene="luxB"
                     /product="LuxB luciferase subunit"
                     /label=LuxB
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MKFGLFFLNFINSTTVQEQSIVRMQEITEYVDKLNFEQILVYENH
                     FSDNGVVGAPLTVSGFLLGLTEKIKIGSLNHIITTHHPVRIAEEACLLDQLSEGRFILG
                     FSDCEKKDEMHFFNRPVEYQQQLFEECYEIINDALTTGYCNPDNDFYSFPKISVNPHAY
                     TPGGPRKYVTATSHHIVEWAAKKGIPLIFKWDDSNDVRYEYAERYKAVADKYDVDLSEI
                     DHQLMILVNYNEDSNKAKQETRAFISDYVLEMHPNENFENKLEEIIAENAVGNYTECIT
                     AAKLAIEKCGAKSVLLSFEPMNDLMSQKNVINIVDDNIKKYHMEYT"
     CDS             8668..9780
                     /codon_start=1
                     /gene="luxE"
                     /product="LuxE"
                     /label=LuxE
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MTSYVDKQEITASSEIDDLIFSSDPLVWSYDEQEKIRKKLVLDAF
                     RNHYKHCREYRHYCQAHKVDDNITEIDDIPVFPTSVFKFTRLLTSQENEIESWFTSSGT
                     NGLKSQVARDRLSIERLLGSVSYGMKYVGSWFDHQIELVNLGPDRFNAHNIWFKYVMSL
                     VELLYPTTFTVTEERIDFVKTLNSLERIKNQGKDLCLIGSPYFIYLLCHYMKDKKISFS
                     GDKSLYIITGGGWKSYEKESLKRDDFNHLLFDTFNLSDISQIRDIFNQVELNTCFFEDE
                     MQRKHVPPWVYARALDPETLKPVPDGTPGLMSYMDASATSYPAFIVTDDVGIISREYGK
                     YPGVLVEILRRVNTRTQKGCALSLTEAFDS"
     primer_bind     9884..9904
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     rep_origin      complement(10015..10603)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10095..10114)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(10772..10858)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     11011..11028
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
ORIGIN
        1 ccttttatgg attcatgcaa ggaaactacc cataatacaa gaaaagcccg tcacgggctt
       61 ctcagggcgt tttatggcgg gtctgctatg tggtgctatc tgactttttg ctgttcagca
      121 gttcctgccc tctgattttc cagtctgacc acttcggatt atcccgtgac aggtcattca
      181 gactggctaa tgcacccagt aaggcagcgg tatcatcaac aggcttaccc gtcttactgt
      241 caaccggatc taaaacacta gcccaacctt tcatagaagg cggcgttgga atcgaaatct
      301 cgtgatggca ggttgggcgt cgcttggtcg gtcatttcga accccagagt cccgctcaga
      361 agaactcgtc aagaaggcga tagaaggcga tgcgctgcga atcgggagcg gcgataccgt
      421 aaagcacgag gaagcggtca gcccattcgc cgccaagctc ttcagcaata tcacgggtag
      481 ccaacgctat gtcctgatag cggtccgcca cacccagccg gccacagtcg atgaatccag
      541 aaaagcggcc attttccacc atgatattcg gcaagcaggc atcgccatgg gtcacgacga
      601 gatcctcgcc gtcgggcatg cgcgccttga gcctggcgaa cagttcggct ggcgcgagcc
      661 cctgatgctc ttcgtccaga tcatcctgat cgacaagacc ggcttccatc cgagtacgtg
      721 ctcgctcgat gcgatgtttc gcttggtggt cgaatgggca ggtagccgga tcaagcgtat
      781 gcagccgccg cattgcatca gccatgatgg atactttctc ggcaggagca aggtgagatg
      841 acaggagatc ctgccccggc acttcgccca atagcagcca gtcccttccc gcttcagtga
      901 caacgtcgag cacagctgcg caaggaacgc ccgtcgtggc cagccacgat agccgcgctg
      961 cctcgtcctg cagttcattc agggcaccgg acaggtcggt cttgacaaaa agaaccgggc
     1021 gcccctgcgc tgacagccgg aacacggcgg catcagagca gccgattgtc tgttgtgccc
     1081 agtcatagcc gaatagcctc tccacccaag cggccggaga acctgcgtgc aatccatctt
     1141 gttcaatcat gcgaaacgat cctcatcctg tctcttgatc agatcttgat cccctgcgcc
     1201 atcagatcct tggcggcaag aaagccatcc agtttacttt gcagggcttc ccaaccttac
     1261 cagagggcgc cccagccgtg gcaattccgg ttcgctgcta gacaacatca gcaaggagaa
     1321 aggggctacc ggcgaaccag cagccccttt ataaaggcgc ttcagtagtc agaccagcat
     1381 cagtcctgaa aaggcgggcc tgcgcccgcc tccaggttgc tacttaccgg attcgtaagc
     1441 catgaaagcc gccacctccc tgtgtccgtc tctgtaacga atctcgcaca gcgattttcg
     1501 tgtcagataa gtgaatatca acagtgtgag acacacgatc aacacacacc agacaaggga
     1561 acttcgtggt agtttcatgg ccttcttctc cttgcgcaaa gcgcggtaag aggctatcct
     1621 gatgtggact agacataggg atgcctcgtg gtggttaatg aaaattaact tactacgggg
     1681 ctatcttctt tctgccacac aacacggcaa caaaccacct tcacgtcatg aggcagaaag
     1741 cctcaagcgc cgggcacatc atagcccata tacctgcacg ctgaccacac tcactttccc
     1801 tgaaaataat ccgctcattc agaccgttca cgggaaatcc gtgtgattgt tgccgcatca
     1861 cgctgcctcc cggagtttgt ctcgagcact tttgttaccc gccaaacaaa acccaaaaac
     1921 aactcatacc caacccaata aaacaccaaa acaagacaaa taatcattga ttgatggttg
     1981 aaatggggta aacttgacaa acaaacccac ttaaaaccca aaacataccc aaacacacac
     2041 caaaaaaaca ccataaggag ttttataaat gttggtattc attgatgacg gttcaacaaa
     2101 catcaaacta cagtagcagg aaagcgacgg aacaattaaa cagcacatta gcccgaacag
     2161 cttcaaacgc gagtgggcag tctcttttgg tgataaaaag gtctttaact acacactgaa
     2221 cggcgaacag tattcatttg atccaatcag cccggatgct gtagtcacaa ccaatatcgc
     2281 atggcaatac agcgacgtta atgtcgttgc agtgcatcac gccttactga ccagtggtct
     2341 gccggtaagc gaagtggata ttgtttgcac acttcctctg acagagtatt acgacagaaa
     2401 taaccaaccc aatacggaaa atattgagcg taagaaagca aacttccgga aaaaaattac
     2461 attaaatggc ggggatacat tcacaataaa agatgtaaaa gtcatgcctg aatctatacc
     2521 ggcaggttat gaagttctac aagaactgga tgagttagat tctttattaa ttatagatct
     2581 cgggggcacc acattagata tttctcaggt aatggggaaa ttatcgggga tcagtaaaat
     2641 atacggagac tcatctcttg gtgtctctct ggttacatct gcagtaaaag atgccctttc
     2701 tcttgcgaga acaaaaggaa gtagctatct tgctgacgat ataatcattc acagaaaaga
     2761 taataactat ctgaagcaac gaattaatga tgagaacgaa atatcaatag tcaccgaagc
     2821 aatgaatgaa gcacttcgta aacttgagca acgtgtatta aatacgctca atgaattttc
     2881 tggttatact catgttatgg ttataggcgg tggcgcagaa ttaatatgcg atgcagtaaa
     2941 aaaacacaca cagattcgtg atgaacgttt tttcaaaacc aataactctc aatatgattt
     3001 agttaacggt atgtatctca taggtaatta atgatggaca agcgcagaac cattgccttc
     3061 aaactaaatc cagatgtaaa tcaaacagat aaaattgttt gtgatacact ggacagtatc
     3121 ccgcaagggg aacgaagccg ccttaaccgg gccgcactga cggcaggtct ggccttatac
     3181 agacaagatc cccggacccc tttcctttta tgtgagctgc tgacgaaaga aaccacattt
     3241 tcagatatcg tgaatatatt gagatcgcta tttccaaaag agatggccga ttttaattct
     3301 tcaatagtca ctcaatcctc ttcacaacaa gagcaaaaaa gtgatgaaga gaccaaaaaa
     3361 aatgcgatga agctaataaa ttaattcaat tattattgag ttccctttat ccactatcag
     3421 gctggataaa gggaactcaa tcaagttatt ttcttaccag tcattacata atcgttatta
     3481 tgaaataatc gtttgcactg tctctgttat tcaggcaatt tcaataaagg cacttgctca
     3541 cgctctgtca ttttctgaaa ctctcatgtg gaattgataa aacagaattt gcctggcggc
     3601 agtagcgcgg tggtcccacc tgaccccatg ccgaactcag aagtgaaacg ccgtagcgcc
     3661 gatggtagtg tggggtctcc ccatgcgaga gtagggaact gccaggcatc aaataaaacg
     3721 aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct
     3781 cctgagtagg acaaatccgc cgggagcgga tttgaacgtt gcgaagcaac ggcccggagg
     3841 gtggcgggca ggacgcccgc cataaactgc caggcatcaa attaagcaga aggccatcct
     3901 gacggatggc ctttttgcgt ttctacaaac tcgaattcat aagaatggat ccaaaataag
     3961 gaggaaaaaa aaatgactag tatgactaaa aaaatttcat tcattattaa cggccaggtt
     4021 gaaatctttc ccgaaggtga tgatttagtg caatccatta attttggtga taatagtgtt
     4081 tacctgccaa tattgaatga ctctcatgta aaaaacatta ttgattgtaa tggaaataac
     4141 gaattacggt tgcataacat tgtcaatttt ctctatacgg tagggcaaag atggaaaaat
     4201 gaagaatact caagacgcag gacatacatt cgtgacttaa aaaaatatat gggatattca
     4261 gaagaaatgg ctaagctaga ggccaattgg atatctatga ttttatgttc taaaggcggc
     4321 ctttatgatg ttgtagaaaa tgaacttggt tctcgccata tcatggatga atggctacct
     4381 caggatgaaa gttatgttcg ggcttttccg aaaggtaaat ctgtacatct gttggcaggt
     4441 aatgttccat tatctgggat catgtctata ttacgcgcaa ttttaactaa gaatcagtgt
     4501 attataaaaa catcgtcaac cgatcctttt accgctaatg cattagcgtt aagttttatt
     4561 gatgtagacc ctaatcatcc gataacgcgc tctttatctg ttatatattg gccccaccaa
     4621 ggtgatacat cactcgcaaa agaaattatg caacatgcgg atgttattgt cgcttgggga
     4681 gggccagatg cgattaattg ggcggtagag catgcgccat cttatgctga tgtgattaaa
     4741 tttggttcta aaaagagtct ttgcattatc gataatcctg ttgatttgac gtccgcagcg
     4801 acaggtgcgg ctcatgatgt ttgtttttac gatcagcgag cttgtttttc tgcccaaaac
     4861 atatattaca tgggaaatca ttatgaggaa tttaagttag cgttgataga aaaacttaat
     4921 ctatatgcgc atatattacc gaatgccaaa aaagattttg atgaaaaggc ggcctattct
     4981 ttagttcaaa aagaaagctt gtttgctgga ttaaaagtag aggtggatat tcatcaacgt
     5041 tggatgatta ttgagtcaaa tgcaggtgtg gaatttaatc aaccacttgg cagatgtgtg
     5101 taccttcatc acgtcgataa tattgagcaa atattgcctt atgttcaaaa aaataagacg
     5161 caaaccatat ctatttttcc ttgggagtca tcatttaaat atcgagatgc gttagcatta
     5221 aaaggtgcgg aaaggattgt agaagcagga atgaataaca tatttcgagt tggtggatct
     5281 catgacggaa tgagaccgtt gcaacgatta gtgacatata tttctcatga aaggccatct
     5341 aactatacgg ctaaggatgt tgcggttgaa atagaacaga ctcgattcct ggaagaagat
     5401 aagttccttg tatttgtccc ataataggta aaaagtatgg aaaatgaatc aaaatataaa
     5461 accatcgacc acgttatttg tgttgaagga aataaaaaaa ttcatgtttg ggaaacgctg
     5521 ccagaagaaa acagcccaaa gagaaagaat gccattatta ttgcgtctgg ttttgcccgc
     5581 aggatggatc attttgctgg tctggcggaa tatttatcgc ggaatggatt tcatgtgatc
     5641 cgctatgatt cgcttcacca cgttggattg agttcaggga caattgatga atttacaatg
     5701 tctataggaa agcagagctt gttagcagtg gttgattggt taactacacg aaaaataaat
     5761 aacttcggta tgttggcttc aagcttatct gcgcggatag cttatgcaag cctatctgaa
     5821 atcaatgctt cgtttttaat caccgcagtc ggtgttgtta acttaagata ttctcttgaa
     5881 agagctttag ggtttgatta tctcagtcta cccattaatg aattgccgaa taatctagat
     5941 tttgaaggcc ataaattggg tgctgaagtc tttgcgagag attgtcttga ttttggttgg
     6001 gaagatttag cttctacaat taataacatg atgtatcttg atataccgtt tattgctttt
     6061 actgcaaata acgataattg ggtcaagcaa gatgaagtta tcacattgtt atcaaatatt
     6121 cgtagtaatc gatgcaagat atattctttg ttaggaagtt cgcatgactt gagtgaaaat
     6181 ttagtggtcc tgcgcaattt ttatcaatcg gttacgaaag ccgctatcgc gatggataat
     6241 gatcatctgg atattgatgt tgatattact gaaccgtcat ttgaacattt aactattgcg
     6301 acagtcaatg aacgccgaat gagaattgag attgaaaatc aagcaatttc tctgtcttaa
     6361 aatctattga gatattctat cactcaaata gcaatataag gactctctat gaaatttgga
     6421 aactttttgc ttacatacca acctccccaa ttttctcaaa cagaggtaat gaaacgtttg
     6481 gttaaattag gtcgcatctc tgaggagtgt ggttttgata ccgtatggtt actggagcat
     6541 catttcacgg agtttggttt gcttggtaac ccttatgtcg ctgctgcata tttacttggc
     6601 gcgactaaaa aattgaatgt aggaactgcc gctattgttc ttcccacagc ccatccagta
     6661 cgccaacttg aagatgtgaa tttattggat caaatgtcaa aaggacgatt tcggtttggt
     6721 atttgccgag ggctttacaa caaggacttt cgcgtattcg gcacagatat gaataacagt
     6781 cgcgccttag cggaatgctg gtacgggctg ataaagaatg gcatgacaga gggatatatg
     6841 gaagctgata atgaacatat caagttccat aaggtaaaag taaaccccgc ggcgtatagc
     6901 agaggtggcg caccggttta tgtggtggct gaatcagctt cgacgactga gtgggctgct
     6961 caatttggcc taccgatgat attaagttgg attataaata ctaacgaaaa gaaagcacaa
     7021 cttgagcttt ataatgaagt ggctcaagaa tatgggcacg atattcataa tatcgaccat
     7081 tgcttatcat atataacatc tgtagatcat gactcaatta aagcgaaaga gatttgccgg
     7141 aaatttctgg ggcattggta tgattcttat gtgaatgcta cgactatttt tgatgattca
     7201 gaccaaacaa gaggttatga tttcaataaa gggcagtggc gtgactttgt attaaaagga
     7261 cataaagata ctaatcgccg tattgattac agttacgaaa tcaatcccgt gggaacgccg
     7321 caggaatgta ttgacataat tcaaaaagac attgatgcta caggaatatc aaatatttgt
     7381 tgtggatttg aagctaatgg aacagtagac gaaattattg cttccatgaa gctcttccag
     7441 tctgatgtca tgccatttct taaagaaaaa caacgttcgc tattatatta gctaaggaga
     7501 aagaaatgaa atttggattg ttcttcctta acttcatcaa ttcaacaact gttcaagaac
     7561 aaagtatagt tcgcatgcag gaaataacgg agtatgttga taagttgaat tttgaacaga
     7621 ttttagtgta tgaaaatcat ttttcagata atggtgttgt cggcgctcct ctgactgttt
     7681 ctggttttct gctcggttta acagagaaaa ttaaaattgg ttcattaaat cacatcatta
     7741 caactcatca tcctgtccgc atagcggagg aagcttgctt attggatcag ttaagtgaag
     7801 ggagatttat tttagggttt agtgattgcg aaaaaaaaga tgaaatgcat ttttttaatc
     7861 gcccggttga atatcaacag caactatttg aagagtgtta tgaaatcatt aacgatgctt
     7921 taacaacagg ctattgtaat ccagataacg atttttatag cttccctaaa atatctgtaa
     7981 atccccatgc ttatacgcca ggcggacctc ggaaatatgt aacagcaacc agtcatcata
     8041 ttgttgagtg ggcggccaaa aaaggtattc ctctcatctt taagtgggat gattctaatg
     8101 atgttagata tgaatatgct gaaagatata aagccgttgc ggataaatat gacgttgacc
     8161 tatcagagat agaccatcag ttaatgatat tagttaacta taacgaagat agtaataaag
     8221 ctaaacaaga gacgcgtgca tttattagtg attatgttct tgaaatgcac cctaatgaaa
     8281 atttcgaaaa taaacttgaa gaaataattg cagaaaacgc tgtcggaaat tatacggagt
     8341 gtataactgc ggctaagttg gcaattgaaa agtgtggtgc gaaaagtgta ttgctgtcct
     8401 ttgaaccaat gaatgatttg atgagccaaa aaaatgtaat caatattgtt gatgataata
     8461 ttaagaagta ccacatggaa tatacctaat agatttcgag ttgcagcgag gcggcaagtg
     8521 aacgaatccc caggagcata gataactatg tgactggggt gagtgaaagc agccaacaaa
     8581 gcagcagctt gaaagatgaa gggtataaaa gagtatgaca gcagtgctgc catactttct
     8641 aatattatct tgaggagtaa aacaggtatg acttcatatg ttgataaaca agaaattaca
     8701 gcaagctcag aaattgatga tttgattttt tcgagcgatc cattagtgtg gtcttacgac
     8761 gagcaggaaa aaatcagaaa gaaacttgtg cttgatgcat ttcgtaatca ttataaacat
     8821 tgtcgagaat atcgtcacta ctgtcaggca cacaaagtag atgacaatat tacggaaatt
     8881 gatgacatac ctgtattccc aacatcggtt tttaagttta ctcgcttatt aacttctcag
     8941 gaaaacgaga ttgaaagttg gtttaccagt agcggcacga atggtttaaa aagtcaggtg
     9001 gcgcgtgaca gattaagtat tgagagactc ttaggctctg tgagttatgg catgaaatat
     9061 gttggtagtt ggtttgatca tcaaatagaa ttagtcaatt tgggaccaga tagatttaat
     9121 gctcataata tttggtttaa atatgttatg agtttggtgg aattgttata tcctacgaca
     9181 tttaccgtaa cagaagaacg aatagatttt gttaaaacat tgaatagtct tgaacgaata
     9241 aaaaatcaag ggaaagatct ttgtcttatt ggttcgccat actttattta tttactctgc
     9301 cattatatga aagataaaaa aatctcattt tctggagata aaagccttta tatcataacc
     9361 ggaggcggct ggaaaagtta cgaaaaagaa tctctgaaac gtgatgattt caatcatctt
     9421 ttatttgata ctttcaatct cagtgatatt agtcagatcc gagatatatt taatcaagtt
     9481 gaactcaaca cttgtttctt tgaggatgaa atgcagcgta aacatgttcc gccgtgggta
     9541 tatgcgcgag cgcttgatcc tgaaacgttg aaacctgtac ctgatggaac gccggggttg
     9601 atgagttata tggatgcgtc agcaaccagt tatccagcat ttattgttac cgatgatgtc
     9661 gggataatta gcagagaata tggtaagtat cccggcgtgc tcgttgaaat tttacgtcgc
     9721 gtcaatacga ggacgcagaa agggtgtgct ttaagcttaa ccgaagcgtt tgatagttga
     9781 tgacctgcag gcatgcaagc ttgcggccgc gggccccatg gatcgatagc tggtcgacac
     9841 aatctgccct ttcgaaagat cccaacgaaa agcgtgacca catggtcctt cttgagtttg
     9901 taactgctgc tgggattaca catggcatgg atgagctcta caaataatga gctagctgaa
     9961 aacctagggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
    10021 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
    10081 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
    10141 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
    10201 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
    10261 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
    10321 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
    10381 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
    10441 cggctacact agaagaacag tatttggtat ctgcgttctg ctgaagccag ttaccttcgg
    10501 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
    10561 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
    10621 ttctacgggg tctgacgctc agtagatcta aaacactagg ccccgtcagg atggccttct
    10681 gcttaatttg atgcctggca gtttatggcg ggcgtcctgc ccgccaccct ccgggccgtt
    10741 gcttcgcaac gttcaaatcc gctcccggcg gatttgtcct actcaggaga gcgttcaccg
    10801 acaaacaaca gataaaacga aaggcccagt ctttcgactg agcctttcgt tttatttgat
    10861 gcctggcagt tccctactct cgcatgggga gaccccacac taccatcggc gctacggcgt
    10921 ttcacttctg agttcggcat ggggtcaggt gggaccaccg cgctactgcc gccaggcaaa
    10981 ttctgtttta tcagaccgct tctgcgttct gatttaatct gtatcaggct gaaaatcttc
    11041 tctcatccgc caaaacagcc aagctggatc tggcaaatcg ctgaatattc cttttgtctc
    11101 cgaccatcag gcacctgagt cgctgtcttt ttcgtgacat tcagttcgct gcgctcacgg
    11161 ctctggcagt gaatgggggt aaatggcact acaggcg
//
