LOCUS       sequence-266141-       11171 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  mammalian expession of GFP-CtlP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11171)
  AUTHORS   Orthwein A, Noordermeer SM, Wilson MD, Landry S, Enchev RI, Sherker 
            A, Munro M, Pinder J, Salsman J, Dellaire G, Xia B, Peter M, 
            Durocher D
  TITLE     A mechanism for the suppression of homologous recombination in G1 
            cells.
  JOURNAL   Nature. 2015 Dec 9. doi: 10.1038/nature16142.
  PUBMED    26649820
REFERENCE   2  (bases 1 to 11171)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2015 Dec 9. doi: 10.1038/nature16142."
COMMENT     Sequence Label: pCW-GFP-CtIP
FEATURES             Location/Qualifiers
     source          1..11171
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    41..158
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        212..526
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    220..238
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    256..274
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    291..309
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    327..345
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    363..381
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    398..416
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    434..452
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     499..523
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    547..571
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      575..584
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(581..583,584..586,587..1297)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(581..583,584..586,587..1297)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(626..647)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(887..906)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1234..1255
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    complement(4044..4068)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             4086..4103
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     promoter        4113..4623
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     4542..4560
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             4633..5229
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4633..4652)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5129..5149
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             5230..5283
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             join(5284..5901,5902..5907,5908..5943,5944..5946,5947..5982,
                     5983..5985,5986..6021,6022..6030)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     primer_bind     complement(6070..6090)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6488..6499)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6704..6937
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7009..7130
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7046..7065)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7100..7119
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7170..7305
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        7305..7376
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7377..7445,7446..8237)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7595..7614)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8408..8996
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8897..8916
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9150..9167
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9284..9305
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9320..9325,9326..9343,9344..9350)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9358..9374
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9363..9385
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9382..9398
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9382..9398
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9417..9437
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        9419..9437
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        9465..9691
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             9692..9872
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    9919..10044
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    10537..10770
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             10955..10999
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 aattctcgac ctcgagacaa atggcagtat tcatccacaa ttttaaaaga aaagggggga
       61 ttggggggta cagtgcaggg gaaagaatag tagacataat agcaacagac atacaaacta
      121 aagaattaca aaaacaaatt acaaaaattc aaaattttcg ggtttattac agggacagca
      181 gagatccact ttggccgcga atcgatatgt cgagtttact ccctatcagt gatagagaac
      241 gtatgtcgag tttactccct atcagtgata gagaacgatg tcgagtttac tccctatcag
      301 tgatagagaa cgtatgtcga gtttactccc tatcagtgat agagaacgta tgtcgagttt
      361 actccctatc agtgatagag aacgtatgtc gagtttatcc ctatcagtga tagagaacgt
      421 atgtcgagtt tactccctat cagtgataga gaacgtatgt cgaggtaggc gtgtacggtg
      481 ggaggcctat ataagcagag ctcgtttagt gaaccgtcag atcgcctgga gaattggcta
      541 gcatcaacaa gtttgtacaa aaaagcaggc tgccgccgcc atggtgagca agggcgagga
      601 gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacaa
      661 gttcagcgtg tccggcgagg gcgagggcga tgccacctac ggcaagctga ccctgaagtt
      721 catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccctgaccta
      781 cggcgtgcag tgcttcagcc gctaccccga ccacatgaag cagcacgact tcttcaagtc
      841 cgccatgccc gaaggctacg tccaggagcg caccatcttc ttcaaggacg acggcaacta
      901 caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa
      961 gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt acaactacaa
     1021 cagccacaac gtctatatca tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa
     1081 gatccgccac aacatcgagg acggcagcgt gcagctcgcc gaccactacc agcagaacac
     1141 ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagca cccagtccgc
     1201 cctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc
     1261 cgccgggatc actctcggca tggacgagct gtacaagtcc ggactcagat ctcgagctca
     1321 agcttcgaat tctgcagtcg acggtaccat gaacatcttg ggaagcagct gtggaagccc
     1381 taactctgca gatacatcta gtgactttaa ggacctttgg acaaaactaa aagaatgtca
     1441 tgatagagaa gcacaaggtt tacaagtaaa agtaaccaag ttgaagcagg aacgaatctt
     1501 agatgcacaa agactagaag aattcttcac caaaaatcaa cagctgaggg aacagcagaa
     1561 agtccttcat gaaaccatta aagttttaga agatcggtta agagcaggct tatgtgatcg
     1621 ctgtgcagta actgaagaac atatgcggaa aaaacagcaa gagtttgaaa atatccggca
     1681 gcagaatctt aaacttatta cagaacttat gaatgaaagg aatactctac aggaagaaaa
     1741 taaaaagctt tctgaacaac tccagcagaa aattgagaat gatcaacagc atcaagcagc
     1801 tgagcttgaa tgtgaggaag acgttattcc agattcaccg ataacagcct tctcattttc
     1861 tggcgttaac cggctacgaa gaaaggagaa cccccatgtc cgatacatag aacaaacaca
     1921 tactaaattg gagcactctg tgtgtgcaaa tgaaatgaga aaagtttcca agtcttcaac
     1981 tcatccacaa cataatccta atgaaaatga aattctagta gctgacactt atgaccaaag
     2041 tcaatctcca atggccaaaa cacatggaac aagcagctat acccctgata agtcatcttt
     2101 taatttagct acagttgttg ctgaaacact tggacttggt gttcaagaag aatctgaaac
     2161 tcaaggtccc atgagccccc ttggtgatga gctctaccac tgtctggaag gaaatcacaa
     2221 gaaacagcct tttgaggaat ctacaagaaa tactgaagat agtttaagat tttcagattc
     2281 tacttcaaag actcctcctc aagaagaatt acctactcga gtgtcatctc ctgtatttgg
     2341 agctacctct agtatcaaaa gtggtttaga tttgaataca agtttgtccc cttctctttt
     2401 acagcctggg aaaaaaaaac atctgaaaac actccctttt agcaacactt gtatatctag
     2461 attagaaaaa actagatcaa aatctgaaga tagtgccctt ttcacacatc acagtcttgg
     2521 gtctgaagtg aacaagatca ttatccagtc atctaataaa cagatactta taaataaaaa
     2581 tataagtgaa tccctaggtg aacagaatag gactgagtac ggtaaagatt ctaacactga
     2641 taaacatttg gagcccctga aatcattggg aggccgaaca tccaaaagga agaaaactga
     2701 ggaagaaagt gaacatgaag taagctgccc ccaagcttct tttgataaag aaaatgcttt
     2761 cccttttcca atggataatc agttttccat gaatggagac tgtgtgatgg ataaacctct
     2821 ggatctgtct gatcgatttt cagctattca gcgtcaagag aaaagccaag gaagtgagac
     2881 ttctaaaaac aaatttaggc aagtgactct ttatgaggct ttgaagacca ttccaaaggg
     2941 cttttcctca agccgtaagg cctcagatgg caactgcatg ttgcccaaag attccccagg
     3001 ggagccctgt tcacaggaat gcatcatcct tcagcccttg aataaatgct ctccagacaa
     3061 taaaccatca ttacaaataa aagaagaaaa tgctgtcttt aaaattcctc tacgtccacg
     3121 tgaaagtttg gagactgaga atgttttaga tgacataaag agtgctggtt ctcatgagcc
     3181 aataaaaata caaaccaggt cagaccatgg aggatgtgaa cttgcatcag ttcttcagtt
     3241 aaatccatgt agaactggta aaataaagtc tctacaaaac aaccaagatg tatcctttga
     3301 aaatatccag tggagtatag atccgggagc agacctttct cagtataaaa tggatgttac
     3361 tgtaatagat acaaaggatg gcagtcagtc aaaattagga ggagagacag tagacatgga
     3421 ctgtacattg gttagtgaaa ccgttctctt aaaaatgaag aaacaagagc agaagggaga
     3481 aaaaagttca aatgaagaaa gaaaaatgaa tgatagcttg gaagatatgt ttgatcggac
     3541 aacacatgaa gagtatgaat cctgtttggc agacagtttc tcccaagcag cagatgaaga
     3601 ggaggaattg tctactgcca caaagaaact acacactcat ggtgataaac aagacaaagt
     3661 caagcagaaa gcgtttgtgg agccgtattt taaaggtgat gaaagagaga ctagcttgca
     3721 aaattttcct catattgagg tggttcggaa aaaagaggag agaagaaaac tgcttgggca
     3781 cacgtgtaag gaatgtgaaa tttattatgc agatatgcca gcagaagaaa gagaaaagaa
     3841 attggcttcc tgctcaagac accgattccg ctacattcca cccaacacac cagagaattt
     3901 ttgggaagtt ggttttcctt ccactcagac ttgtatggaa agaggttata ttaaggaaga
     3961 tcttgatcct tgtcctcgtc caaaaagacg tcagccttac aacgcaatat tttctccaaa
     4021 aggcaaggag cagaagacat aggacccagc tttcttgtac aaagtggttt agtaatgaac
     4081 cggtccacca ccaccaccac cactaaggat ccggggttgg ggttgcgcct tttccaaggc
     4141 agccctgggt ttgcgcaggg acgcggctgc tctgggcgtg gttccgggaa acgcagcggc
     4201 gccgaccctg ggtctcgcac attcttcacg tccgttcgca gcgtcacccg gatcttcgcc
     4261 gctacccttg tgggcccccc ggcgacgctt cctgctccgc ccctaagtcg ggaaggttcc
     4321 ttgcggttcg cggcgtgccg gacgtgacaa acggaagccg cacgtctcac tagtaccctc
     4381 gcagacggac agcgccaggg agcaatggca gcgcgccgac cgcgatgggc tgtggccaat
     4441 agcggctgct cagcagggcg cgccgagagc agcggccggg aaggggcggt gcgggaggcg
     4501 gggtgtgggg cggtagtgtg ggccctgttc ctgcccgcgc ggtgttccgc attctgcaag
     4561 cctccggagc gcacgtcggc agtcggctcc ctcgttgacc gaatcaccga cctctctccc
     4621 cagcaattca ccatgaccga gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc
     4681 cccagggccg tacgcaccct cgccgccgcg ttcgccgact accccgccac gcgccacacc
     4741 gtcgatccgg accgccacat cgagcgggtc accgagctgc aagaactctt cctcacgcgc
     4801 gtcgggctcg acatcggcaa ggtgtgggtc gcggacgacg gcgccgcggt ggcggtctgg
     4861 accacgccgg agagcgtcga agcgggggcg gtgttcgccg agatcggccc gcgcatggcc
     4921 gagttgagcg gttcccggct ggccgcgcag caacagatgg aaggcctcct ggcgccgcac
     4981 cggcccaagg agcccgcgtg gttcctggcc accgtcggcg tctcgcccga ccaccagggc
     5041 aagggtctgg gcagcgccgt cgtgctcccc ggagtggagg cggccgagcg cgccggggtg
     5101 cccgccttcc tggagacctc cgcgccccgc aacctcccct tctacgagcg gctcggcttc
     5161 accgtcaccg ccgacgtcga ggtgcccgaa ggaccgcgca cctggtgcat gacccgcaag
     5221 cccggtgccg aaggtagagg ttctctcctc acttgtggtg atgttgaaga aaaccctggt
     5281 ccaatgtcta gactggacaa gagcaaagtc ataaacggag ctctggaatt actcaatggt
     5341 gtcggtatcg aaggcctgac gacaaggaaa ctcgctcaaa agctgggagt tgagcagcct
     5401 accctgtact ggcacgtgaa gaacaagcgg gccctgctcg atgccctgcc aatcgagatg
     5461 ctggacaggc atcataccca cttctgcccc ctggaaggcg agtcatggca agactttctg
     5521 cggaacaacg ccaagtcata ccgctgtgct ctcctctcac atcgcgacgg ggctaaagtg
     5581 catctcggca cccgcccaac agagaaacag tacgaaaccc tggaaaatca gctcgcgttc
     5641 ctgtgtcagc aaggcttctc cctggagaac gcactgtacg ctctgtccgc cgtgggccac
     5701 tttacactgg gctgcgtatt ggaggaacag gagcatcaag tagcaaaaga ggaaagagag
     5761 acacctacca ccgattctat gcccccactt ctgagacaag caattgagct gttcgaccgg
     5821 cagggagccg aacctgcctt ccttttcggc ctggaactaa tcatatgtgg cctggagaaa
     5881 cagctaaagt gcgaaagcgg cgggccgacc gacgcccttg acgattttga cttagacatg
     5941 ctcccagccg atgcccttga cgactttgac cttgatatgc tgcctgctga cgctcttgac
     6001 gattttgacc ttgacatgct ccccgggtaa ctaagtaagg atcgatccaa gatatcgtat
     6061 tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca
     6121 tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc
     6181 tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc
     6241 tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt
     6301 cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg
     6361 gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga agctgacgtc
     6421 ctttccatgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta
     6481 cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg
     6541 gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc
     6601 cccgcctgtt tcgcctcggc gtccggacta gaggtacctt taagaccaat gacttacaag
     6661 gcagctgtag atcttagcca ctttttaaaa gaaaaggggg gactggaagg gctaattcac
     6721 tcccaacgaa gacaagatct gctttttgct tgtactgggt ctctctggtt agaccagatc
     6781 tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg
     6841 ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc
     6901 ctcagaccct tttagtcagt gtggaaaatc tctagcagta gtagttcatg tcatcttatt
     6961 attcagtatt tataacttgc aaagaaatga atatcagaga gtgagaggaa cttgtttatt
     7021 gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt
     7081 ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg
     7141 ctctagctat cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc
     7201 attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg
     7261 cctctgagct attccagaag tagtgaggag gcttttttgg aggcctttca aatatgtatc
     7321 cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga
     7381 gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt
     7441 ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag
     7501 tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag
     7561 aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta
     7621 ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg
     7681 agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca
     7741 gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag
     7801 gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc
     7861 gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg
     7921 tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc
     7981 ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg
     8041 cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg
     8101 gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga
     8161 cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac
     8221 tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa
     8281 aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca
     8341 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
     8401 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
     8461 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
     8521 ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc
     8581 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
     8641 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
     8701 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
     8761 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
     8821 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
     8881 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
     8941 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
     9001 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
     9061 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata
     9121 ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc
     9181 gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg
     9241 acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca
     9301 ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg
     9361 tgagcggata acaatttcac acaggaaaca gctatgacca tgattacgcc aagcgcgcaa
     9421 ttaaccctca ctaaagggaa caaaagctgg agctgcaagc ttaatgtagt cttatgcaat
     9481 actcttgtag tcttgcaaca tggtaacgat gagttagcaa catgccttac aaggagagaa
     9541 aaagcaccgt gcatgccgat tggtggaagt aaggtggtac gatcgtgcct tattaggaag
     9601 gcaacagacg ggtctgacat ggattggacg aaccactgaa ttgccgcatt gcagagatat
     9661 tgtatttaag tgcctagctc gatacataaa cgggtctctc tggttagacc agatctgagc
     9721 ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg
     9781 agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag
     9841 acccttttag tcagtgtgga aaatctctag cagtggcgcc cgaacaggga cttgaaagcg
     9901 aaagggaaac cagaggagct ctctcgacgc aggactcggc ttgctgaagc gcgcacggca
     9961 agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt tgactagcgg aggctagaag
    10021 gagagagatg ggtgcgagag cgtcagtatt aagcggggga gaattagatc gcgatgggaa
    10081 aaaattcggt taaggccagg gggaaagaaa aaatataaat taaaacatat agtatgggca
    10141 agcagggagc tagaacgatt cgcagttaat cctggcctgt tagaaacatc agaaggctgt
    10201 agacaaatac tgggacagct acaaccatcc cttcagacag gatcagaaga acttagatca
    10261 ttatataata cagtagcaac cctctattgt gtgcatcaaa ggatagagat aaaagacacc
    10321 aaggaagctt tagacaagat agaggaagag caaaacaaaa gtaagaccac cgcacagcaa
    10381 gcggccgctg atcttcagac ctggaggagg agatatgagg gacaattgga gaagtgaatt
    10441 atataaatat aaagtagtaa aaattgaacc attaggagta gcacccacca aggcaaagag
    10501 aagagtggtg cagagagaaa aaagagcagt gggaatagga gctttgttcc ttgggttctt
    10561 gggagcagca ggaagcacta tgggcgcagc gtcaatgacg ctgacggtac aggccagaca
    10621 attattgtct ggtatagtgc agcagcagaa caatttgctg agggctattg aggcgcaaca
    10681 gcatctgttg caactcacag tctggggcat caagcagctc caggcaagaa tcctggctgt
    10741 ggaaagatac ctaaaggatc aacagctcct ggggatttgg ggttgctctg gaaaactcat
    10801 ttgcaccact gctgtgcctt ggaatgctag ttggagtaat aaatctctgg aacagatttg
    10861 gaatcacacg acctggatgg agtgggacag agaaattaac aattacacaa gcttaataca
    10921 ctccttaatt gaagaatcgc aaaaccagca agaaaagaat gaacaagaat tattggaatt
    10981 agataaatgg gcaagtttgt ggaattggtt taacataaca aattggctgt ggtatataaa
    11041 attattcata atgatagtag gaggcttggt aggtttaaga atagtttttg ctgtactttc
    11101 tatagtgaat agagttaggc agggatattc accattatcg tttcagaccc acctcccaac
    11161 cccgagggga c
//
