LOCUS       sequence-26615-e         782 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 782)
  AUTHORS   Funakoshi M, Hochstrasser M
  TITLE     Small epitope-linker modules for PCR-based C-terminal tagging in 
            Saccharomyces cerevisiae.
  JOURNAL   Yeast. 2009 Feb 25. 26(3):185-192.
  PUBMED    19243080
REFERENCE   2  (bases 1 to 782)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast. 2009
            Feb 25. 26(3):185-192."
COMMENT     Sequence Label: pFA6a-6xGLY-His-tag-HIS3MX6
FEATURES             Location/Qualifiers
     source          1..782
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(79..276)
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     CDS             complement(570..584)
                     /codon_start=1
                     /product="tag for protein cleavage using Ni2+ ion (Dang et 
                     al., 2019)"
                     /label=SNAC tag
                     /translation="GSHHW"
ORIGIN
        1 ggcnnctagt gganctgata tcatcgatga attcgagctc gtttaaactg gatggcggcg
       61 ttagtatcga atcgacagca gtatagcgac cagcattcac atacgattga cgcatgatat
      121 tactttctgc gcacttaact tcgcatctgg gcagatgatg tcgaggcgaa aaaaaatata
      181 aatcacgcta acatttgatt aaaatagaac aactacaata taaaaaaact atacaaatga
      241 caagttcttg aaaacaagaa tctttttatt gtcagtactt cacatcaaaa cacctttggt
      301 tgagggaacg tcattggtgc cattgctaga aatagcttct cttatggcaa cagccaaagc
      361 cttgaacgca ctctcacttc tgtggtgatc gttgaaacct ctcagacaat caacatgcaa
      421 agtaattctg gccgcctccg cgaaactttc caaaaagtgt ggaatcattt cagtggataa
      481 atcaccaatc atctctctct tcaatccaag gtcgattaca gcaaatggtc tactagataa
      541 atcgactacg gcacgtgata gcgcctcatc caatggtgcg aacccagtac cgaatctttt
      601 tacaccacgg acagcaccca ttgcttcttt gaacgcttgc cctaatgcga taccgcaatc
      661 ttcggtagta tggtgatcgt caatgtgcan gtcaccaata cattcnacaa taagagacca
      721 accagagtgt tttgccaacg catggatcat atgatccaaa aagccaacac ntgtgtgaat
      781 at
//
