LOCUS       sequence-266154-        2664 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pZA2-pLlacO-soxR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2664)
  AUTHORS   Muller IE, Rubens JR, Jun T, Graham D, Xavier R, Lu TK
  TITLE     Gene networks that compensate for crosstalk with crosstalk.
  JOURNAL   Nat Commun. 2019 Sep 6;10(1):4028. doi: 10.1038/s41467-019-12021-y.
  PUBMED    31492904
REFERENCE   2  (bases 1 to 2664)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2019-09-6"; volume: "10(1):4028. doi"; pages: " 
            10.1038/s41467-019-12021-y"
COMMENT     Sequence Label: fIM1265
FEATURES             Location/Qualifiers
     source          1..2664
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    8..24
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    31..47
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             99..110
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     terminator      611..697
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     complement(734..751)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(868..1413)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(948..967)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1525..1619)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(1650..2444)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(1761..1780)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     2371..2390
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     2623..2641
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 ctagcaattg tgagcggata acaattgaca ttgtgagcgg ataacaagat actgagcaca
       61 tcagcaggac gcactgacct taattaaaag aattcattaa agaggagaaa ggtaccatgg
      121 aaaagaaatt accccgcatt aaagcgctgc taacccccgg cgaagtggcg aaacgcagcg
      181 gtgtggcggt atcggcgctg catttctatg aaagtaaagg gttgattacc agtatccgta
      241 acagcggcaa tcagcggcga tataaacgtg atgtgttgcg atatgttgca attatcaaaa
      301 ttgctcagcg tattggcatt ccgctggcga ccattggtga agcgtttggc gtgttgcccg
      361 aagggcatac gttaagtgcg aaagagtgga aacagctttc gtcccaatgg cgagaagagt
      421 tggatcggcg cattcatacc ttagtggcgc tgcgtgacga actggacgga tgtattggtt
      481 gtggctgcct ttcgcgcagt gattgcccgt tgcgtaaccc gggcgaccgc ttaggagaag
      541 aaggtaccgg cgcacgcttg ctggaagatg aacaaaacta aggatcccat ggtacgcgtg
      601 ctagaggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc gttttatctg
      661 ttgtttgtcg gtgaacgctc tcctgagtag gacaaatccg ccgccctaga cctaggggga
      721 tatattccgc ttcctcgctc actgactcgc tacgctcggt cgttcgactg cggcgagcgg
      781 aaatggctta cgaacggggc ggagatttcc tggaagatgc caggaagata cttaacaggg
      841 aagtgagagg gccgcggcaa agccgttttt ccataggctc cgcccccctg acaagcatca
      901 cgaaatctga cgctcaaatc agtggtggcg aaacccgaca ggactataaa gataccaggc
      961 gtttccccct ggcggctccc tcgtgcgctc tcctgttcct gcctttcggt ttaccggtgt
     1021 cattccgctg ttatggccgc gtttgtctca ttccacgcct gacactcagt tccgggtagg
     1081 cagttcgctc caagctggac tgtatgcacg aaccccccgt tcagtccgac cgctgcgcct
     1141 tatccggtaa ctatcgtctt gagtccaacc cggaaagaca tgcaaaagca ccactggcag
     1201 cagccactgg taattgattt agaggagtta gtcttgaagt catgcgccgg ttaaggctaa
     1261 actgaaagga caagttttgg tgactgcgct cctccaagcc agttacctcg gttcaaagag
     1321 ttggtagctc agagaacctt cgaaaaaccg ccctgcaagg cggttttttc gttttcagag
     1381 caagagatta cgcgcagacc aaaacgatct caagaagatc atcttattaa tcagataaaa
     1441 tatttctaga tttcagtgca atttatctct tcaaatgtag cacctgaagt cagccccata
     1501 cgatataagt tgtatagtgc ttggattctc accaataaaa aacgcccggc ggcaaccgag
     1561 cgttctgaac aaatccagat ggagttctga ggtcattact ggatctatca acaggagtcc
     1621 aagcgagctc tcgaacccca gagtcccgct cagaagaact cgtcaagaag gcgatagaag
     1681 gcgatgcgct gcgaatcggg agcggcgata ccgtaaagca cgaggaagcg gtcagcccat
     1741 tcgccgccaa gctcttcagc aatatcacgg gtagccaacg ctatgtcctg atagcggtcc
     1801 gccacaccca gccggccaca gtcgatgaat ccagaaaagc ggccattttc caccatgata
     1861 ttcggcaagc aggcatcgcc atgggtcacg acgagatcct cgccgtcggg catgcgcgcc
     1921 ttgagcctgg cgaacagttc ggctggcgcg agcccctgat gctcttcgtc cagatcatcc
     1981 tgatcgacaa gaccggcttc catccgagta cgtgctcgct cgatgcgatg tttcgcttgg
     2041 tggtcgaatg ggcaggtagc cggatcaagc gtatgcagcc gccgcattgc atcagccatg
     2101 atggatactt tctcggcagg agcaaggtga gatgacagga gatcctgccc cggcacttcg
     2161 cccaatagca gccagtccct tcccgcttca gtgacaacgt cgagcacagc tgcgcaagga
     2221 acgcccgtcg tggccagcca cgatagccgc gctgcctcgt cctgcagttc attcagggca
     2281 ccggacaggt cggtcttgac aaaaagaacc gggcgcccct gcgctgacag ccggaacacg
     2341 gcggcatcag agcagccgat tgtctgttgt gcccagtcat agccgaatag cctctccacc
     2401 caagcggccg gagaacctgc gtgcaatcca tcttgttcaa tcatgcgaaa cgatcctcat
     2461 cctgtctctt gatcagatct tgatcccctg cgccatcaga tccttggcgg caagaaagcc
     2521 atccagttta ctttgcaggg cttcccaacc ttaccagagg gcgccccagc tggcaattcc
     2581 gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg tatcacgagg
     2641 ccctttcgtc ttcacctcga gatg
//
