LOCUS       sequence-266156-        4165 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pZS2-proD-soxR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4165)
  AUTHORS   Muller IE, Rubens JR, Jun T, Graham D, Xavier R, Lu TK
  TITLE     Gene networks that compensate for crosstalk with crosstalk.
  JOURNAL   Nat Commun. 2019 Sep 6;10(1):4028. doi: 10.1038/s41467-019-12021-y.
  PUBMED    31492904
REFERENCE   2  (bases 1 to 4165)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2019-09-6"; volume: "10(1):4028. doi"; pages: " 
            10.1038/s41467-019-12021-y"
COMMENT     Sequence Label: pIM11
FEATURES             Location/Qualifiers
     source          1..4165
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      677..763
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             complement(1308..2258)
                     /codon_start=1
                     /gene="rep101"
                     /product="RepA protein needed for replication with the 
                     pSC101 origin"
                     /label=Rep101
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLHNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKYDLNGSFSWLTQKQRTTLENILAKYGRI"
     rep_origin      2306..2528
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     terminator      complement(3026..3120)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(3151..3945)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3262..3281)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     3872..3891
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4124..4142
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 ctagccacag ctaacaccac gtcgtcccta tctgctgccc taggtctatg agtggttgct
       61 ggataacttt acgggcatgc ataaggctcg tataatatat tcagggagac cacaacggtt
      121 tccctctaca aataattttg tttaactttg aattcttcac acaggaaacc ggtaccatgg
      181 aaaagaaatt accccgcatt aaagcgctgc taacccccgg cgaagtggcg aaacgcagcg
      241 gtgtggcggt atcggcgctg catttctatg aaagtaaagg gttgattacc agtatccgta
      301 acagcggcaa tcagcggcga tataaacgtg atgtgttgcg atatgttgca attatcaaaa
      361 ttgctcagcg tattggcatt ccgctggcga ccattggtga agcgtttggc gtgttgcccg
      421 aagggcatac gttaagtgcg aaagagtgga aacagctttc gtcccaatgg cgagaagagt
      481 tggatcggcg cattcatacc ttagtggcgc tgcgtgacga actggacgga tgtattggtt
      541 gtggctgcct ttcgcgcagt gattgcccgt tgcgtaaccc gggcgaccgc ttaggagaag
      601 aaggtaccgg cgcacgcttg ctggaagatg aacaaaacta acccggggga tcccatggta
      661 cgcgtgctag aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt
      721 tatctgttgt ttgtcggtga acgctctcct gagtaggaca aatccgccgc cctagaccta
      781 gggcctaggg tacgggtttt gctgcccgca aacgggctgt tctggtgttg ctagtttgtt
      841 atcagaatcg cagatccggc ttcagccggt ttgccggctg aaagcgctat ttcttccaga
      901 attgccatga ttttttcccc acgggaggcg tcactggctc ccgtgttgtc ggcagctttg
      961 attcgataag cagcatcgcc tgtttcaggc tgtctatgtg tgactgttga gctgtaacaa
     1021 gttgtctcag gtgttcaatt tcatgttcta gttgctttgt tttactggtt tcacctgttc
     1081 tattaggtgt tacatgctgt tcatctgtta cattgtcgat ctgttcatgg tgaacagctt
     1141 tgaatgcacc aaaaactcgt aaaagctctg atgtatctat cttttttaca ccgttttcat
     1201 ctgtgcatat ggacagtttt ccctttgata tgtaacggtg aacagttgtt ctacttttgt
     1261 ttgttagtct tgatgcttca ctgatagata caagagccat aagaacctca gatccttccg
     1321 tatttagcca gtatgttctc tagtgtggtt cgttgttttt gcgtgagcca tgagaacgaa
     1381 ccattgagat catacttact ttgcatgtca ctcaaaaatt ttgcctcaaa actggtgagc
     1441 tgaatttttg cagttaaagc atcgtgtagt gtttttctta gtccgttatg taggtaggaa
     1501 tctgatgtaa tggttgttgg tattttgtca ccattcattt ttatctggtt gttctcaagt
     1561 tcggttacga gatccatttg tctatctagt tcaacttgga aaatcaacgt atcagtcggg
     1621 cggcctcgct tatcaaccac caatttcata ttgctgtaag tgtttaaatc tttacttatt
     1681 ggtttcaaaa cccattggtt aagcctttta aactcatggt agttattttc aagcattaac
     1741 atgaacttaa attcatcaag gctaatctct atatttgcct tgtgagtttt cttttgtgtt
     1801 agttctttta ataaccactc ataaatcctc atagagtatt tgttttcaaa agacttaaca
     1861 tgttccagat tatattttat gaattttttt aactggaaaa gataaggcaa tatctcttca
     1921 ctaaaaacta attctaattt ttcgcttgag aacttggcat agtttgtcca ctggaaaatc
     1981 tcaaagcctt taaccaaagg attcctgatt tccacagttc tcgtcatcag ctctctggtt
     2041 gctttagcta atacaccata agcattttcc ctactgatgt tcatcatctg agcgtattgg
     2101 ttataagtga acgataccgt ccgttctttc cttgtagggt tttcaatcgt ggggttgagt
     2161 agtgccacac agcataaaat tagcttggtt tcatgctccg ttaagtcata gcgactaatc
     2221 gctagttcat ttgctttgaa aacaactaat tcagacatac atctcaattg gtctaggtga
     2281 ttttaatcac tataccaatt gagatgggct agtcaatgat aattactagt ccttttcctt
     2341 tgagttgtgg gtatctgtaa attctgctag acctttgctg gaaaacttgt aaattctgct
     2401 agaccctctg taaattccgc tagacctttg tgtgtttttt ttgtttatat tcaagtggtt
     2461 ataatttata gaataaagaa agaataaaaa aagataaaaa gaatagatcc cagccctgtg
     2521 tataactcac tactttagtc agttccgcag tattacaaaa ggatgtcgca aacgctgttt
     2581 gctcctctac aaaacagacc ttaaaaccct aaaggcttaa gtagcaccct cgcaagctcg
     2641 ggcaaatcgc tgaatattcc ttttgtctcc gaccatcagg cacctgagtc gctgtctttt
     2701 tcgtgacatt cagttcgctg cgctcacggc tctggcagtg aatgggggta aatggcacta
     2761 caggcgcctt ttatggattc atgcaaggaa actacccata atacaagaaa agcccgtcac
     2821 gggcttctca gggcgtttta tggcgggtct gctatgtggt gctatctgac tttttgctgt
     2881 tcagcagttc ctgccctctg attttccagt ctgaccactt cggattatcc cgtgacaggt
     2941 cattcagact ggctaatgca cccagtaagg cagcggtatc atcaacaggc ttacccgtct
     3001 tactgtccct agtactagtg cttggattct caccaataaa aaacgcccgg cggcaaccga
     3061 gcgttctgaa caaatccaga tggagttctg aggtcattac tggatctatc aacaggagtc
     3121 caagcgagct ctcgaacccc agagtcccgc tcagaagaac tcgtcaagaa ggcgatagaa
     3181 ggcgatgcgc tgcgaatcgg gagcggcgat accgtaaagc acgaggaagc ggtcagccca
     3241 ttcgccgcca agctcttcag caatatcacg ggtagccaac gctatgtcct gatagcggtc
     3301 cgccacaccc agccggccac agtcgatgaa tccagaaaag cggccatttt ccaccatgat
     3361 attcggcaag caggcatcgc catgggtcac gacgagatcc tcgccgtcgg gcatgcgcgc
     3421 cttgagcctg gcgaacagtt cggctggcgc gagcccctga tgctcttcgt ccagatcatc
     3481 ctgatcgaca agaccggctt ccatccgagt acgtgctcgc tcgatgcgat gtttcgcttg
     3541 gtggtcgaat gggcaggtag ccggatcaag cgtatgcagc cgccgcattg catcagccat
     3601 gatggatact ttctcggcag gagcaaggtg agatgacagg agatcctgcc ccggcacttc
     3661 gcccaatagc agccagtccc ttcccgcttc agtgacaacg tcgagcacag ctgcgcaagg
     3721 aacgcccgtc gtggccagcc acgatagccg cgctgcctcg tcctgcagtt cattcagggc
     3781 accggacagg tcggtcttga caaaaagaac cgggcgcccc tgcgctgaca gccggaacac
     3841 ggcggcatca gagcagccga ttgtctgttg tgcccagtca tagccgaata gcctctccac
     3901 ccaagcggcc ggagaacctg cgtgcaatcc atcttgttca atcatgcgaa acgatcctca
     3961 tcctgtctct tgatcagatc ttgatcccct gcgccatcag atccttggcg gcaagaaagc
     4021 catccagttt actttgcagg gcttcccaac cttaccagag ggcgccccag ctggcaattc
     4081 cgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc gtatcacgag
     4141 gccctttcgt cttcacctcg agatg
//
