LOCUS       sequence-266169-        5881 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  To generate a replicative plasmid carrying mycobacteriophage L5 
            integrase.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5881)
  AUTHORS   Boldrin F, Anoosheh S, Serafini A, Cioetto Mazzabo L, Palu G, 
            Provvedi R, Manganelli R
  TITLE     Improving the stability of the TetR/Pip-OFF mycobacterial 
            repressible promoter system.
  JOURNAL   Sci Rep. 2019 Apr 8;9(1):5783. doi: 10.1038/s41598-019-42319-2.
  PUBMED    30962489
REFERENCE   2  (bases 1 to 5881)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2019-04-8"; volume: "9(1):5783. doi"; pages: " 
            10.1038/s41598-019-42319-2"
COMMENT     Sequence Label: pANTO3
FEATURES             Location/Qualifiers
     source          1..5881
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1..816
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPHAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHNLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(74..93)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      1147..1735
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1636..1655
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      3888..3934
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     complement(join(5871..5881,1..9))
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
ORIGIN
        1 atgagccata ttcaacggga aacgtcttgc tcgaggccgc gattaaattc caacatggat
       61 gctgatttat atgggtataa atgggctcgc gataatgtcg ggcaatcagg tgcgacaatc
      121 tatcgcttgt atgggaagcc ccatgcgcca gagttgtttc tgaaacatgg caaaggtagc
      181 gttgccaatg atgttacaga tgagatggtc agactaaact ggctgacgga atttatgcct
      241 cttccgacca tcaagcattt tatccgtact cctgatgatg catggttact caccactgcg
      301 atccccggga aaacagcatt ccaggtatta gaagaatatc ctgattcagg tgaaaatatt
      361 gttgatgcgc tggcagtgtt cctgcgccgg ttgcattcga ttcctgtttg taattgtcct
      421 tttaacagcg atcgcgtatt tcgtctcgct caggcgcaat cacgaatgaa taacggtttg
      481 gttgatgcga gtgattttga tgacgagcgt aatggctggc ctgttgaaca agtctggaaa
      541 gaaatgcata atcttttgcc attctcaccg gattcagtcg tcactcatgg tgatttctca
      601 cttgataacc ttatttttga cgaggggaaa ttaataggtt gtattgatgt tggacgagtc
      661 ggaatcgcag accgatacca ggatcttgcc atcctatgga actgcctcgg tgagttttct
      721 ccttcattac agaaacggct ttttcaaaaa tatggtattg ataatcctga tatgaataaa
      781 ttgcagtttc atttgatgct cgatgagttt ttctaatcag aattggttaa ttggttgtaa
      841 cactggcaga gcattacgct gacttgacgg gacggcggct ttgttgaata aatcgaactt
      901 ttgctgagtt gaaggatcag atcacgcatc ttcccgacaa cgcagaccgt tccgtggcaa
      961 agcaaaagtt caaaatcacc aactggtcca cctacaacaa agctctcacc aaccgtggct
     1021 ccctcacttt ctggctggat gatggggcga ttcaggcctg gtatgagtca gcaacacctt
     1081 cttcacgagg cagacctcac tagttccatg agcgtcagac cccgtagaaa agatcaaagg
     1141 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
     1201 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac
     1261 tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca
     1321 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
     1381 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
     1441 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
     1501 aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc
     1561 cgaggggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
     1621 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
     1681 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
     1741 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt
     1801 tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac
     1861 cgctcgccgc agccgaacga ccgagcgcaa cgcgtgagcc caccagctcc gtaagttcgg
     1921 gcgctgtgtg gctcgtaccc gcgcattcag gcggcagggg gtctaacggg tctaaggcgg
     1981 cgtgtacggc cgccacagcg gctctcagcg gcccggaaac gtcctcgaaa cgacgcatgt
     2041 gttcctcctg gttggtacag gtggttgggg gtgctcggct gtcgctggtg ttccaccacc
     2101 agggctcgac gggagagcgg gggagtgtgc agttgtgggg tggcccctca gcgaaatatc
     2161 tgacttggag ctcgtgtcgg accatacacc ggtgattaat cgtggtctac taccaagcgt
     2221 gagccacgtc gccgacgaat ttgagcagct ctggctgccg tactggccgc tggcaagcga
     2281 cgatctgctc gaggggatct accgccaaag ccgcgcgtcg gccctaggcc gccggtacat
     2341 cgaggcgaac ccaacagcgc tggcaaacct gctggtcgtg gacgtagacc atccagacgc
     2401 agcgctccga gcgctcagcg cccgggggtc ccatccgctg cccaacgcga tcgtgggcaa
     2461 tcgcgccaac ggccacgcac acgcagtgtg ggcactcaac gcccctgttc cacgcaccga
     2521 atacgcgcgg cgtaagccgc tcgcatacat ggcggcgtgc gccgaaggcc ttcggcgcgc
     2581 cgtcgatggc gaccgcagtt actcaggcct catgaccaaa aaccccggcc acatcgcctg
     2641 ggaaacggaa tggctccact cagatctcta cacactcagc cacatcgagg ccgagctcgg
     2701 cgcgaacatg ccaccgccgc gctggcgtca gcagaccacg tacaaagcgg ctccgacgcc
     2761 gctagggcgg aattgcgcac tgttcgattc cgtcaggttg tgggcctatc gtcccgccct
     2821 catgcggatc tacctgccga cccggaacgt ggacggactc ggccgcgcga tctatgccga
     2881 gtgccacgcg cgaaacgccg aatttccgtg caacgacgtg tgtcccggac cgctaccgga
     2941 cagcgaggtc cgcgccatcg ccaacagcat ttggcgttgg atcacaacca agtcgcgcat
     3001 ttgggcggac gggatcgtgg tctacgaggc cacactcagt gcgcgccagt cggccatctc
     3061 gcggaagggc gcagcagcgc gcacggcggc gagcacagtt gcgcggcgcg caaagtccgc
     3121 gtcagccatg gaggcattgc tatgagcgac ggctacagcg acggctacaa ccggcagccg
     3181 actgtccgca aaaagcggcg cgtgaccgcc gccgaaggcg ctcgaatcac cggactatcc
     3241 gaacgccacg tcgtccggct cgtggcgcag gaacgcagcg agtggctcgc cgagcaggct
     3301 gcacgccgcg aacgcatccg cgcctatcac gacgacgagg gccactcttg gccgcaaacg
     3361 gccaaacatt tcgggctgca tctggacacc gttaagcgac tcggctatcg ggcgaggaaa
     3421 gagcgtgcgg cagaacagga agcggctcaa aaggcccaca acgaagccga caatccaccg
     3481 ctgttctaac gcaattgggg agcgggtgtc gcgggggttc cgtggggggt tccgttgcaa
     3541 cgggtcggac aggtaaaagt cctggtagac gctagttttc tggtttgggc catgcctgtc
     3601 tcgttgcgtg tttcgttgcg cccgttttga ataccagcca gacgagacgg ggttctacga
     3661 atcttggtcg ataccaagcc atttccgctg aatatcgggg agctcaccgc cagaatcggt
     3721 ggttgtggtg atgtacgtgg cgaactccgt tgtagtgcct gtggtggcat ccgtggcgcg
     3781 gccgcggtac cagatcttta aatctagata tccatggatc cagctgcaga attcgaagct
     3841 tatcgatgtc gacgtagtta actagcgtac gatcgactgc caggcatcaa ataaaacgaa
     3901 aggctcagtc gaaagactgg gcctttcgtt ttatgccatc atggccgcgg tgatcagcta
     3961 gagccgtgaa cgacagggcg aacgccagcc cgccgacggc gagggttccg accgctgcaa
     4021 ctcccggtgc aaccttgtcc cggtctattc tcttcactgc accagctcca atctggtgtg
     4081 aatgcccctc gtctgttcgc gcaggcgggg ggctctattc gtttgtcagc atcgaaagta
     4141 gccagatcag ggatgcgttg caaccgcgta tgcccaggtc agaagagtcg cacaagagtt
     4201 gcagacccct ggaaagaaaa atggccagag ggcgaaaaca ccctctgacc agcggagcgg
     4261 gcgacgggaa tcgaacccgc gtagctagtt tggaagaatg ggtgtctgcc gaccacatat
     4321 atgggccggt caagataggt ttttaccccc tctcggctgc atcctctaag tggaaagaaa
     4381 ttgcaggtcg tagaagcgcg ttgaagcctg agagttgcac aggagttgca acccggtagc
     4441 cttgttcacg acgagaggag acctagttgg cacgtcgcgg atggggatcg ctgaagactc
     4501 agcgcagcgg gaggatccaa gcctcatacg tcaacccgca ggacggtgtg aggtactacg
     4561 cgctgcagac ctacgacaac aagatggacg ccgaagcctg gctcgcgggc gagaagcggc
     4621 tcatcgagat ggagacctgg acccctccac aggaccgggc gaagaaggca gccgccagcg
     4681 ccatcacgct ggaggagtac acccggaagt ggctcgtgga gcgcgacctc gcagacggca
     4741 ccagggatct gtacagcggg cacgcggagc gccgcatcta cccggtgcta ggtgaagtgg
     4801 cggtcacaga gatgacgcca gctctggtgc gtgcgtggtg ggccgggatg ggtaggaagc
     4861 acccgactgc ccgccggcat gcctacaacg tcctccgggc ggtgatgaac acagcggtcg
     4921 aggacaagct gatcgcagag aacccgtgcc ggatcgagca gaaggcagcc gatgagcgcg
     4981 acgtagaggc gctgacgcct gaggagctgg acatcgtcgc cgctgagatc ttcgtgcact
     5041 accggatcgc ggcatacatc ctggcgtgga cgagcctccg gttcggagag ctgatcgagc
     5101 ttcgccgcaa ggacatcgtg gacgacggca tgacgatgaa gctccgggtg cgccgtggcg
     5161 cttcccgcgt ggggaacaag atcgtcgttg gcaacgccaa gaccgtccgg tcgaagcgtc
     5221 ctgtgacggt tccgcctcac gtcgcggaga tgatccgagc gcacatgaag gaccgtacga
     5281 agatgaacaa gggccccgag gcattcctgg tgaccacgac gcagggcaac cggctgtcga
     5341 agtccgcgtt caccaagtcg ctgaagcgtg gctacgccaa gatcggtcgg ccggaactcc
     5401 gcatccacga cctccgcgct gtcggcgcta cgttcgccgc tcaggcaggt gcgacgacca
     5461 aggagctgat ggcccgtctc ggtcacacga ctcctaggat ggcgatgaag taccagatgg
     5521 cgtctgaggc ccgcgacgag gctatcgctg aggcgatgtc caagctggcc aagacctcct
     5581 gaaacgcaaa aagcccccct cccaaggaca ctgagtccta aagagggggg tttcttgtca
     5641 gtacgcgaag aaccacgcct ggccgcgagc gccagcaccg ccgctctgtg cggagacctg
     5701 ggcaccagcc ccgccgccgc caggagcatt gccgttcccg ccagctagcc acctgacgtc
     5761 gggggggggg gaaagccacg ttgtgtctca aaatctctga tgttacattg cacaagataa
     5821 aaatatatca tcatgaacaa taaaactgtc tgcttacata aacagtaata caaggggtgt
     5881 t
//
