LOCUS       sequence-266170-        7140 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7140)
  AUTHORS   Xi Q, He W, Zhang XH, Le HV, Massague J
  TITLE     Genome-wide impact of the BRG1 SWI/SNF chromatin remodeler on the 
            transforming growth factor beta transcriptional program.
  JOURNAL   J Biol Chem. 2008 Jan 11. 283(2):1146-55.
  PUBMED    18003620
REFERENCE   2  (bases 1 to 7140)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2008 Jan 11. 283(2):1146-55."
COMMENT     Sequence Label: pCMV BAF170-Flag
FEATURES             Location/Qualifiers
     source          1..7140
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     126..148
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     140..157
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     141..157
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     enhancer        318..697
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        698..901
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     852..872
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             4582..4605
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     protein_bind    4625..4646
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4763..4780)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4934..5522)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5014..5033)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5693..6484,6485..6553))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6316..6335
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6554..6658)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      6685..7113
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6772..6791)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6982..7003
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct
       61 attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg
      121 gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc aagctgatct atacattgaa
      181 tcaatattgg caattagcca tattagtcat tggttatata gcataaatca atattggcta
      241 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc
      301 aatatgaccg ccatgttgac attgattatt gactagttat taatagtaat caattacggg
      361 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
      421 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
      481 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
      541 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga
      601 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg
      661 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac
      721 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
      781 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaataaccc
      841 cgccccgttg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc
      901 tcgtttagtg aaccgtcaga attccccgag ccggagaaga tggcggtgcg gaagaaggac
      961 ggcggcccca acgtgaagta ctacgaggcc gcggacaccg tgacccagtt cgacaacgtg
     1021 cggctgtggc tcggcaagaa ctacaagaag tatatacaag ctgaaccacc caccaacaag
     1081 tccctgtcta gcctggttgt acagttgcta caatttcagg aagaagtttt tggcaaacat
     1141 gtcagcaatg caccgctcac taaactgccg atcaaatgtt tcctagattt caaagcggga
     1201 ggctccttgt gccacattct tgcagctgcc tacaaattca agagtgacca gggatggcgg
     1261 cgttacgatt tccagaatcc atcacgcatg gaccgcaatg tggaaatgtt tatgaccatt
     1321 gagaagtcct tggtgcagaa taattgcctg tctcgaccta acatttttct gtgcccagaa
     1381 attgagccca aactactagg gaaattaaag gacattatca agagacacca gggaacagtc
     1441 actgaggata agaacaatgc ctcccatgtt gtgtatcctg tcccggggaa tctagaagaa
     1501 gaggaatggg tacgaccagt catgaagagg gataagcagg ttcttctgca ctggggctac
     1561 tatcctgaca gttacgacac gtggatccca gcgagtgaaa ttgaggcatc tgtggaagat
     1621 gctccaactc ctgagaaacc taggaaggtt catgcaaagt ggatcctgga caccgacacc
     1681 ttcaatgaat ggatgaatga ggaagactat gaagtaaatg atgacaaaaa ccctgtctcc
     1741 cgccgaaaga agatttcagc caagacactg acagatgagg tgaacagccc agattcagat
     1801 cgacgggaca agaagggggg aaactataag aagaggaagc gctccccctc tccttcacca
     1861 accccagaag caaagaagaa aaatgctaag aaaggtccct caacacctta cactaagtca
     1921 aagcgtggcc acagagaaga ggagcaagaa gacctgacaa aggacatgga cgagccctca
     1981 ccagtcccca atgtagaaga ggtgacactt cccaaaacag tcaacacaaa gaaagactca
     2041 gagtcggccc cagtcaaagg cggcaccatg accgacctgg atgaacagga agatgaaagc
     2101 atggagacga cgggcaagga tgaggatgag aacagtacgg ggaacaaggg agagcagacc
     2161 aagaatccag acctgcatga ggacaatgtg actgaacaga cccaccacat catcattccc
     2221 agctacgctg cctggtttga ctacaatagt gttcatgcca ttgagcggag ggctctcccc
     2281 gagttcttca acggcaagaa caagtccaag actccagaga tctacctggc ctatcgaaac
     2341 tttatgattg acacttaccg actgaacccc caagagtatc ttacctctac cgcctgccgc
     2401 cgaaacctag cgggtgatgt ctgtgccatc atgagggtcc atgccttcct agaacagtgg
     2461 ggtcttatta actaccaggt ggatgctgag agtcgaccaa ccccaatggg gcctccgcct
     2521 acctctcact tccatgtctt ggctgacaca ccatcagggc tggtgcctct gcagcccaag
     2581 acacctcagc agacctctgc ttcccaacaa atgctcaact ttcctgacaa aggcaaagag
     2641 aaaccaacag acatgcaaaa ctttgggctg cgcacagaca tgtacacaaa aaagaatgtt
     2701 ccctccaaga gcaaggctgc agccagtgcc actcgtgagt ggacagaaca ggaaaccctg
     2761 cttctcctgg aggcactgga aatgtacaaa gatgactgga acaaagtgtc cgagcatgtg
     2821 ggaagccgca cacaggacga gtgcatcttg cattttcttc gtcttcccat tgaagaccca
     2881 tacctggagg actcagaggc ctccctaggc cccctggcct accaacccat ccccttcagt
     2941 cagtcgggca accctgttat gagcactgtt gccttcctgg cctctgtcgt cgatccccga
     3001 gtcgcctctg ctgctgcaaa gtcagcccta gaggagttct ccaaaatgaa ggaagaggta
     3061 cccacggcct tggtggaggc ccatgttcga aaagtggaag aagcagccaa agtaacaggc
     3121 aaggcggacc ctgccttcgg tctggaaagc agtggcattg caggaaccac ctctgatgag
     3181 cctgagcgga ttgaggagag cgggaatgac gaggctcggg tggaaggcca ggccacagat
     3241 gagaagaagg agcccaagga accccgagaa ggagggggtg ctatagagga ggaagcaaaa
     3301 gagaaaacca gcgaggctcc caagaaggat gaggagaaag ggaaagaagg cgacagtgag
     3361 aaggagtccg agaagagtga tggagaccca atagtcgatc ctgagaagga gaaggagcca
     3421 aaggaagggc aggaggaagt gctgaaggaa gtggtggagt ctgaggggga aaggaagaca
     3481 aaggtggagc gggacattgg cgagggcaac ctctccaccg ctgctgccgc cgccctggcc
     3541 gccgccgcag tgaaagctaa gcacttggct gctgttgagg aaaggaagat caaatctttg
     3601 gtggccctgc tggtggagac ccagatgaaa aagttggaga tcaaacttcg gcactttgag
     3661 gagctggaga ctatcatgga ccgggagcga gaagcactgg agtatcagag gcagcagctc
     3721 ctggccgaca gacaagcctt ccacatggag cagctgaagt atgcggagat gagggctcgg
     3781 cagcagcact tccaacagat gcaccaacag cagcagcagc caccaccagc cctgccccca
     3841 ggctcccagc ctatcccccc aacaggggct gctgggccac ccgcagtcca tggcttggct
     3901 gtggctccag cctctgtagt ccctgctcct gctggcagtg gggcccctcc aggaagtttg
     3961 ggcccttctg aacagattgg gcaggcaggg tcaactgcag ggccacagca gcagcaacca
     4021 gctggagccc cccagcctgg ggcagtccca ccaggggttc ccccccctgg accccatggc
     4081 ccctcaccgt tccccaacca acaaactcct ccctcaatga tgccaggggc agtgccaggc
     4141 agcgggcacc caggcgtggc gggtaatgct cctttgggtt tgccttttgg catgccgcct
     4201 cctcctcctc ctcctgctcc atccatcatc ccatttggta gtctagctga ctccatcagt
     4261 attaacctcc ccgctcctcc taacctgcat gggcatcacc accatctccc gttcgccccg
     4321 ggcactctcc ccccacctaa cctgcctgtg tccatggcga accctctaca tcctaacctg
     4381 ccggcgacca ccaccatgcc atcttccttg cctctcgggc cggggctcgg atccgccgca
     4441 gcccaaagcc ctgccattgt ggcagctgtt cagggcaacc tcctgcccag tgccagccca
     4501 ctgccagacc caggcacccc cctgcctcca gaccccacag ccccgagccc aggcacggtc
     4561 acccctgtgc cacctccaca ggactacaag gacgacgatg acaagtgaaa gcttggggtg
     4621 cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg
     4681 gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc
     4741 gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
     4801 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
     4861 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
     4921 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     4981 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     5041 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     5101 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
     5161 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     5221 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     5281 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     5341 tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc
     5401 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
     5461 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
     5521 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
     5581 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
     5641 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
     5701 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
     5761 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
     5821 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
     5881 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
     5941 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
     6001 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
     6061 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
     6121 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
     6181 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
     6241 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
     6301 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
     6361 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
     6421 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
     6481 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
     6541 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
     6601 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
     6661 catttccccg aaaagtgcca cctgacgcgc cctgtagcgg cgcattaagc gcggcgggtg
     6721 tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg
     6781 ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg
     6841 ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt
     6901 agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt
     6961 tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta
     7021 tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa
     7081 atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg cttacaattt
//
