LOCUS       sequence-266182-         822 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  A photoactivatable GEVI variant originated from Bongwoori-R3.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 822)
  AUTHORS   Lee S, Song YK, Baker BJ
  TITLE     Engineering Photoactivatability in Genetically Encoded Voltage and 
            pH Indicators.
  JOURNAL   Front Cell Neurosci. 2019 Oct 29;13:482. doi: 
            10.3389/fncel.2019.00482. eCollection 2019.
  PUBMED    31736711
REFERENCE   2  (bases 1 to 822)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front Cell 
            Neurosci."; date: "2019-10-29"; volume: "13:482. doi"; pages: " 
            10.3389/fncel.2019.00482. eCollection 2019"
COMMENT     Sequence Label: PA-ecliptic pHluorin
FEATURES             Location/Qualifiers
     source          1..822
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     15..34
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        15..33
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             53..769
                     /codon_start=1
                     /product="pH-sensitive mutant of green fluorescent protein 
                     (Miesenbock et al., 1998)"
                     /label=ecliptic pHluorin
                     /note="responds to a drop in pH with a gradual loss 
                     (eclipsing) of excitation at 475 nm, accompanied by a 
                     reduction of excitation at 395 nm"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFSYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNDHQVYIMADKQKNGIKA
                     NFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLFHTSTLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(83..111)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     706..726
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
ORIGIN
        1 tggcttatcg aaattaatac gactcactat agggagaccc aagctggcta gcatgagtaa
       61 aggagaagaa cttttcactg gagttgtccc aattcttgtt gaattagatg gtgatgttaa
      121 tgggcacaaa ttttctgtca gtggagaggg tgaaggtgat gcaacatacg gaaaacttac
      181 ccttaaattt atttgcacta ctggaaaact acctgttcca tggccaacac ttgtcactac
      241 tttctcttat ggtgttcaat gcttttcaag atacccagat catatgaaac ggcatgactt
      301 tttcaagagt gccatgcccg aaggttatgt acaggaaaga actatatttt tcaaagatga
      361 cgggaactac aagacacgtg ctgaagtcaa gtttgaaggt gatacccttg ttaatagaat
      421 cgagttaaaa ggtattgatt ttaaagaaga tggaaacatt cttggacaca aattggaata
      481 caactataac gatcaccagg tgtacatcat ggcagacaaa caaaagaatg gaatcaaagc
      541 taacttcaaa attagacaca acattgaaga tggaggcgtt caactagcag accattatca
      601 acaaaatact ccaattggcg atgggcccgt ccttttacca gacaaccatt acctgtttca
      661 cacttctact ctttcgaaag atcccaacga aaagagagac cacatggtcc ttcttgagtt
      721 tgtaacagct gccgggatta cacatggcat ggatgaacta tacaaataat ctagaactcg
      781 agtctagagg gcccgtttaa acccgctgat cagcctcgac tg
//
