LOCUS       sequence-266185-       11836 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  BeYDV viral replicon on T-DNA backbone expressing Firefly Luc+.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11836)
  AUTHORS   Maher MF, Nasti RA, Vollbrecht M, Starker CG, Clark MD, Voytas DF
  TITLE     Plant gene editing through de novo induction of meristems.
  JOURNAL   Nat Biotechnol. 2019 Dec 16. pii: 10.1038/s41587-019-0337-2. doi: 
            10.1038/s41587-019-0337-2.
  PUBMED    31844292
REFERENCE   2  (bases 1 to 11836)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2019 Dec 16. pii: 10.1038/s41587-019-0337-2. doi: 
            10.1038/s41587-019-0337-2."
COMMENT     Sequence Label: pMKV060
FEATURES             Location/Qualifiers
     source          1..11836
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1037..1231
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(1467..1489)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    1575..1715
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     1630..1649
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(1730..1747)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1901..2489)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1981..2000)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(2576..3370)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    3795..3819
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             5657..7309
                     /codon_start=1
                     /gene="luc+"
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="enhanced luc+ version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     primer_bind     complement(5723..5741)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     misc_feature    9351..9375
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             10676..11305
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             join(11734..11836,1..971)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="VSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
ORIGIN
        1 tcgaggcaga agcacgcccc ggtgaatcgt ggcaagcggc cgctgatcga atccgcaaag
       61 aatcccggca accgccggca gccggtgcgc cgtcgattag gaagccgccc aagggcgacg
      121 agcaaccaga ttttttcgtt ccgatgctct atgacgtggg cacccgcgat agtcgcagca
      181 tcatggacgt ggccgttttc cgtctgtcga agcgtgaccg acgagctggc gaggtgatcc
      241 gctacgagct tccagacggg cacgtagagg tttccgcagg gccggccggc atggccagtg
      301 tgtgggatta cgacctggta ctgatggcgg tttcccatct aaccgaatcc atgaaccgat
      361 accgggaagg gaagggagac aagcccggcc gcgtgttccg tccacacgtt gcggacgtac
      421 tcaagttctg ccggcgagcc gatggcggaa agcagaaaga cgacctggta gaaacctgca
      481 ttcggttaaa caccacgcac gttgccatgc agcgtacgaa gaaggccaag aacggccgcc
      541 tggtgacggt atccgagggt gaagccttga ttagccgcta caagatcgta aagagcgaaa
      601 ccgggcggcc ggagtacatc gagatcgagc tagctgattg gatgtaccgc gagatcacag
      661 aaggcaagaa cccggacgtg ctgacggttc accccgatta ctttttgatc gatcccggca
      721 tcggccgttt tctctaccgc ctggcacgcc gcgccgcagg caaggcagaa gccagatggt
      781 tgttcaagac gatctacgaa cgcagtggca gcgccggaga gttcaagaag ttctgtttca
      841 ccgtgcgcaa gctgatcggg tcaaatgacc tgccggagta cgatttgaag gaggaggcgg
      901 ggcaggctgg cccgatccta gtcatgcgct accgcaacct gatcgagggc gaagcatccg
      961 ccggttccta atgtacggag cagatgctag ggcaaattgc cctagcaggg gaaaaaggtc
     1021 gaaaaggcct ctttcctgtg gatagcacgt acattgggaa cccaaagccg tacattggga
     1081 accggaaccc gtacattggg aacccaaagc cgtacattgg gaaccggtca cacatgtaag
     1141 tgactgatat aaaagagaaa aaaggcgatt tttccgccta aaactcttta aaacttatta
     1201 aaactcttaa aacccgcctg gcctgtgcat aactgtctgg ccagcgcaca gccgaagagc
     1261 tgcaaaaagc gcctaccctt cggtcgctgc gctccctacg ccccgccgct tcgcgtcggc
     1321 ctatcgcggc cgctggccgc tcaaaaatgg ctggcctacg gccaggcaat ctaccagggc
     1381 gcggacaagc cgcgccgtcg ccactcgacc gccggcgccc acatcaaggc accctgcctc
     1441 gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc agctcccgga aacggtcaca
     1501 gcttgtctgt aagcggatgc cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt
     1561 ggcgggtgtc ggggcgcagc catgacccag tcacgtagcg atagcggagt gtatactggc
     1621 ttaactatgc ggcatcagag cagattgtac tgagagtgca ccatatgcgg tgtgaaatac
     1681 cgcacagatg cgtaaggaga aaataccgca tcaggcgctc ttccgcttcc tcgctcactg
     1741 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa
     1801 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc
     1861 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
     1921 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
     1981 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
     2041 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
     2101 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
     2161 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
     2221 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
     2281 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
     2341 ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
     2401 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
     2461 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
     2521 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgcattct aggtactaaa
     2581 acaattcatc cagtaaaata taatatttta ttttctccca atcaggcttg atccccagta
     2641 agtcaaaaaa tagctcgaca tactgttctt ccccgatatc ctccctgatc gaccggacgc
     2701 agaaggcaat gtcataccac ttgtccgccc tgccgcttct cccaagatca ataaagccac
     2761 ttactttgcc atctttcaca aagatgttgc tgtctcccag gtcgccgtgg gaaaagacaa
     2821 gttcctcttc gggcttttcc gtctttaaaa aatcatacag ctcgcgcgga tctttaaatg
     2881 gagtgtcttc ttcccagttt tcgcaatcca catcggccag atcgttattc agtaagtaat
     2941 ccaattcggc taagcggctg tctaagctat tcgtataggg acaatccgat atgtcgatgg
     3001 agtgaaagag cctgatgcac tccgcataca gctcgataat cttttcaggg ctttgttcat
     3061 cttcatactc ttccgagcaa aggacgccat cggcctcact catgagcaga ttgctccagc
     3121 catcatgccg ttcaaagtgc aggacctttg gaacaggcag ctttccttcc agccatagca
     3181 tcatgtcctt ttcccgttcc acatcatagg tggtcccttt ataccggctg tccgtcattt
     3241 ttaaatatag gttttcattt tctcccacca gcttatatac cttagcagga gacattcctt
     3301 ccgtatcttt tacgcagcgg tatttttcga tcagtttttt caattccggt gatattctca
     3361 ttttagccat ttattatttc cttcctcttt tctacagtat ttaaagatac cccaagaagc
     3421 taattataac aagacgaact ccaattcact gttccttgca ttctaaaacc ttaaatacca
     3481 gaaaacagct ttttcaaagt tgttttcaaa gttggcgtat aacatagtat cgacggagcc
     3541 gattttgaaa ccgcggtgat cacaggcagc aacgctctgt catcgttaca atcaacatgc
     3601 taccctccgc gagatcatcc gtgtttcaaa cccggcagct tagttgccgt tcttccgaat
     3661 agcatcggta acatgagcaa agtctgccgc cttacaacgg ctctcccgct gacgccgtcc
     3721 cggactgatg ggctgcctgt atcgagtggt gattttgtgc cgagctgccg gtcggggagc
     3781 tgttggctgg ctggtggcag gatatattgt ggtgtaaaca aattgacgct tagacaactt
     3841 aataacacat tgcggacgtt tttaatgtag agctcaaagt ttaacgcgtt agcagaaggc
     3901 atgttgttgt gactccgagg ggttgcctca aactctatct tataaccggc gtggaggcat
     3961 ggaggcaggg gtattttggt cattttaata gatagtggaa aatgacgtgg aatttactta
     4021 aagacgaagt ctttgcgaca agggggggcc cacgccgaat ttaatattac cggcgtggcc
     4081 cccccttatc gcgagtgctt tagcacgagc ggtccagatt taaagtagaa aatttcccgc
     4141 ccactagggt taaaggtgtt cacactataa aagcatatac gatgtgatgg tatttgatgg
     4201 agcgtatatt gtatcaggta tttccgttgg atacgaatta ttcgtacgac cctcggtacc
     4261 gatcggcgcg cgcggccgca cagagattta aatagctccg gtgacggacg gcgcgcccta
     4321 tgtcgagctg caggtcaacg gatcaggata ttcttgttta agatgttgaa ctctatggag
     4381 gtttgtatga actgatgatc taggaccgga taagttccct tcttcatagc gaacttattc
     4441 aaagaatgtt ttgtgtatca ttcttgttac attgttatta atgaaaaaat attattggtc
     4501 attggactga acacgagtgt taaatatgga ccaggcccca aataagatcc attgatatat
     4561 gaattaaata acaagaataa atcgagtcac caaaccactt gcctttttta acgagacttg
     4621 ttcaccaact tgatacaaaa gtcattatcc tatgcaaatc aataatcata caaaaatatc
     4681 caataacact aaaaaattaa aagaaatgga taatttcaca atatgttata cgataaagaa
     4741 gttacttttc caagaaattc actgatttta taagcccact tgcattagat aaatggcaaa
     4801 aaaaaacaaa aaggaaaaga aataaagcac gaagaattct agaaaatacg aaatacgctt
     4861 caatgcagtg ggacccacgg ttcaattatt gccaattttc agctccaccg tatatttaaa
     4921 aaataaaacg ataatgctaa aaaaatataa atcgtaacga tcgttaaatc tcaacggctg
     4981 gatcttatga cgaccgttag aaattgtggt tgtcgacgag tcagtaataa acggcgtcaa
     5041 agtggttgca gccggcacac acgagtcgtg tttatcaact caaagcacaa atacttttcc
     5101 tcaacctaaa aataaggcaa ttagccaaaa acaactttgc gtgtaaacaa cgctcaatac
     5161 acgtgtcatt ttattattag ctattgcttc accgccttag ctttctcgtg acctagtcgt
     5221 cctcgtcttt tcttcttctt cttctataaa acaataccca aagagctctt cttcttcaca
     5281 attcagattt caatttctca aaatcttaaa aactttctct caattctctc taccgtgatc
     5341 aaggtaaatt tctgtgttcc ttattctctc aaaatcttcg attttgtttt cgttcgatcc
     5401 caatttcgta tatgttcttt ggtttagatt ctgttaatct tagatcgaac acgattttct
     5461 gggtttgatc gttagatatc atcttaattc tcgattaggg tttcatagat atcatccgat
     5521 ttgttcaaat aatttgagtt ttgtcgaata attactcttc gatttgtgat ttctatctag
     5581 atctggtgtt agtttctagt ttgtgcgatc gaatttgtcg attaatctga gtttttctga
     5641 ttaacaggcc tgcaggatgg aagacgccaa aaacataaag aaaggcccgg cgccattcta
     5701 tccgctggaa gatggaaccg ctggagagca actgcataag gctatgaaga gatacgccct
     5761 ggttcctgga acaattgctt ttacagatgc acatatcgag gtggacatca cttacgctga
     5821 gtacttcgaa atgtccgttc ggttggcaga agctatgaaa cgatatgggc tgaatacaaa
     5881 tcacagaatc gtcgtatgca gtgaaaactc tcttcaattc tttatgccgg tgttgggcgc
     5941 gttatttatc ggagttgcag ttgcgcccgc gaacgacatt tataatgaac gtgaattgct
     6001 caacagtatg ggcatttcgc agcctaccgt ggtgttcgtt tccaaaaagg ggttgcaaaa
     6061 aattttgaac gtgcaaaaaa agctcccaat catccaaaaa attattatca tggattctaa
     6121 aacggattac cagggatttc agtcgatgta cacgttcgtc acatctcatc tacctcccgg
     6181 ttttaatgaa tacgattttg tgccagagtc cttcgatagg gacaagacaa ttgcactgat
     6241 catgaactcc tctggatcta ctggtctgcc taaaggtgtc gctctgcctc atagaactgc
     6301 ctgcgtgaga ttctcgcatg ccagagatcc tatttttggc aatcaaatca ttccggatac
     6361 tgcgatttta agtgttgttc cattccatca cggttttgga atgtttacta cactcggata
     6421 tttgatatgt ggatttcgag tcgtcttaat gtatagattt gaagaagagc tgtttctgag
     6481 gagccttcag gattacaaga ttcaaagtgc gctgctggtg ccaaccctat tctccttctt
     6541 cgccaaaagc actctgattg acaaatacga tttatctaat ttacacgaaa ttgcttctgg
     6601 tggcgctccc ctctctaagg aagtcgggga agcggttgcc aagaggttcc atctgccagg
     6661 tatcaggcaa ggatatgggc tcactgagac tacatcagct attctgatta cacccgaggg
     6721 ggatgataaa ccgggcgcgg tcggtaaagt tgttccattt tttgaagcga aggttgtgga
     6781 tctggatacc gggaaaacgc tgggcgttaa tcaaagaggc gaactgtgtg tgagaggtcc
     6841 tatgattatg tccggttatg taaacaatcc ggaagcgacc aacgccttga ttgacaagga
     6901 tggatggcta cattctggag acatagctta ctgggacgaa gacgaacact tcttcatcgt
     6961 tgaccgcctg aagtctctga ttaagtacaa aggctatcag gtggctcccg ctgaattgga
     7021 atccatcttg ctccaacacc ccaacatctt cgacgctggt gtcgcaggtc ttcccgacga
     7081 tgacgccggt gaacttcccg ccgccgttgt tgttttggag cacggaaaga cgatgacgga
     7141 aaaagagatc gtggattacg tcgccagtca agtaacaacc gcgaaaaagt tgcgcggagg
     7201 agttgtgttt gtggacgaag taccgaaagg tcttaccgga aaactcgacg caagaaaaat
     7261 cagagagatc ctcataaagg ccaagaaggg cggaaagatc gccgtgtgac tcgaggttcg
     7321 agtattatgg cattgggaaa actgtttttc ttgtaccatt tgttgtgctt gtaatttact
     7381 gtgtttttta ttcggttttc gctatcgaac tgtgaaatgg aaatggatgg agaagagtta
     7441 atgaatgata tggtcctttt gttcattctc aaattaatat tatttgtttt ttctcttatt
     7501 tgttgtgtgt tgaatttgaa attataagag atatgcaaac attttgtttt gagtaaaaat
     7561 gtgtcaaatc gtggcctcta atgaccgaag ttaatatgag gagtaaaaca cttgtagttg
     7621 tgttagagct cccggggcgc gccgatatcg agctctcccg gcgcgccgat atcgagctca
     7681 gtgtttgatc gccggcggta ccgagtgtac ttcaagtcag tgggaaatca ataaaatgat
     7741 tattttatga atatatttca ttgtgcaagt agatagaaat tacatatgtt acataacaca
     7801 cgaaataaac aaaaaaagac aatccaaaaa caaacacccc aaaaaaaata atcactttag
     7861 ataaactcgt atgaggagag gcacgttcag tgactcgacg attcccgagc aaaaaaagtc
     7921 tccccgtcac acatgtagtg ggtgacgcaa ttatctttaa agtaatcctt ctgttgactt
     7981 gtcattgata acatccagtc ttcgtcagga ttgcaaagaa ttatagaagg gatcccacct
     8041 tttattttct tcttttttcc atatttaggg ttgacagtga aatcagactg gcaacctatt
     8101 aattgcttcc acaatgggac gaacttgaag gggatgtcgt cgatgatatt ataggtggcg
     8161 tgttcatcgt agttggtgaa atcgatggta ccgttccaat agttgtgtcg tccgagactt
     8221 ctagcccagg tggtctttcc ggtacgagtt ggtccgcaga tgtagaggct ggggtgtcgg
     8281 attccattcc ttccattgtc cttgttaaat cggccatcca ttcaaggtca gattgagctt
     8341 gttggtatga gacaggatgt atgtaagtat aagcgtctat gcttacatgg tatagatggg
     8401 tttccctcca ggagtgtaga tcttcgtggc agcgaagatc tgattctgtg aagggcgaca
     8461 catacggttc aggttgtgga gggaataatt tgttggctga atattccagc cattgaagct
     8521 ttgttgccca ttcatgaggg aattcttcct tgatcatgtc aagatattcc tccttagacg
     8581 ttgcagtctg gataatagtt ctccatcgtg cgtcagattt gcgaggagaa accttatgat
     8641 ctcggaaatc tcctctggtt ttaatatctc cgtcctttga tatgtaatca aggacttgtt
     8701 tagagtttct agctggctgg atattagggt gatttccttc aaaatcgaaa aaagaaggat
     8761 ccctaataca aggtttttta tcaagctgga gaagagcatg atagtgggta gtgccatctt
     8821 gatgaagctc agaagcaaca ccaaggaaga aaataagaaa aggtgtgagt ttctcccaga
     8881 gaaactggaa taaatcatct ctttgagatg agcacttggg ataggtaagg aaaacatatt
     8941 tagattggag tctgaagttc ttactagcag aaggcatgtt gttgtgactc cgaggggttg
     9001 cctcaaactc tatcttataa ccggcgtgga ggcatggagg caggggtatt ttggtcattt
     9061 taatagatag tggaaaatga cgtggaattt acttaaagac gaagtctttg cgacaagggg
     9121 gggcccacgc cgaatttaat attaccggcg tggccccccc ttatcgcgag tgctttagca
     9181 cgagcggtcc agatttaaag tagaaaattt cccgcccact agggttaaag gtgttcacac
     9241 tataaaagca tatacgatgt gatggtattt gatggagcgt atattgtatc aggtatttcc
     9301 gttggatacg aattattcgt acgaccctca tagtttaaac tatcagtgtt tgacaggata
     9361 tattggcggg taaacctaag agaaaagagc gtttattaga ataatcggat atttaaaagg
     9421 gcgtgaaaag gtttatccgt tcgtccattt gtatgtgcat gccaaccaca gggttcccct
     9481 cgggatcaaa gtactttgat ccaacccctc cgctgctata gtgcagtcgg cttctgacgt
     9541 tcagtgcagc cgtcttctga aaacgacatg tcgcacaagt cctaagttac gcgacaggct
     9601 gccgccctgc ccttttcctg gcgttttctt gtcgcgtgtt ttagtcgcat aaagtagaat
     9661 acttgcgact agaaccggag acattacgcc atgaacaaga gcgccgccgc tggcctgctg
     9721 ggctatgccc gcgtcagcac cgacgaccag gacttgacca accaacgggc cgaactgcac
     9781 gcggccggct gcaccaagct gttttccgag aagatcaccg gcaccaggcg cgaccgcccg
     9841 gagctggcca ggatgcttga ccacctacgc cctggcgacg ttgtgacagt gaccaggcta
     9901 gaccgcctgg cccgcagcac ccgcgaccta ctggacattg ccgagcgcat ccaggaggcc
     9961 ggcgcgggcc tgcgtagcct ggcagagccg tgggccgaca ccaccacgcc ggccggccgc
    10021 atggtgttga ccgtgttcgc cggcattgcc gagttcgagc gttccctaat catcgaccgc
    10081 acccggagcg ggcgcgaggc cgccaaggcc cgaggcgtga agtttggccc ccgccctacc
    10141 ctcaccccgg cacagatcgc gcacgcccgc gagctgatcg accaggaagg ccgcaccgtg
    10201 aaagaggcgg ctgcactgct tggcgtgcat cgctcgaccc tgtaccgcgc acttgagcgc
    10261 agcgaggaag tgacgcccac cgaggccagg cggcgcggtg ccttccgtga ggacgcattg
    10321 accgaggccg acgccctggc ggccgccgag aatgaacgcc aagaggaaca agcatgaaac
    10381 cgcaccagga cggccaggac gaaccgtttt tcattaccga agagatcgag gcggagatga
    10441 tcgcggccgg gtacgtgttc gagccgcccg cgcacggctc aaccgtgcgg ctgcatgaaa
    10501 tcctggccgg tttgtctgat gccaagctgg cggcctggcc ggccagcttg gccgctgaag
    10561 aaaccgagcg ccgccgtcta aaaaggtgat gtgtatttga gtaaaacagc ttgcgtcatg
    10621 cggtcgctgc gtatatgatg cgatgagtaa ataaacaaat acgcaagggg aacgcatgaa
    10681 ggttatcgct gtacttaacc agaaaggcgg gtcaggcaag acgaccatcg caacccatct
    10741 agcccgcgcc ctgcaactcg ccggggccga tgttctgtta gtcgattccg atccccaggg
    10801 cagtgcccgc gattgggcgg ccgtgcggga agatcaaccg ctaaccgttg tcggcatcga
    10861 ccgcccgacg attgaccgcg acgtgaaggc catcggccgg cgcgacttcg tagtgatcga
    10921 cggagcgccc caggcggcgg acttggctgt gtccgcgatc aaggcagccg acttcgtgct
    10981 gattccggtg cagccaagcc cttacgacat atgggccacc gccgacctgg tggagctggt
    11041 taagcagcgc attgaggtca cggatggaag gctacaagcg gcctttgtcg tgtcgcgggc
    11101 gatcaaaggc acgcgcatcg gcggtgaggt tgccgaggcg ctggccgggt acgagctgcc
    11161 cattcttgag tcccgtatca cgcagcgcgt gagctaccca ggcactgccg ccgccggcac
    11221 aaccgttctt gaatcagaac ccgagggcga cgctgcccgc gaggtccagg cgctggccgc
    11281 tgaaattaaa tcaaaactca tttgagttaa tgaggtaaag agaaaatgag caaaagcaca
    11341 aacacgctaa gtgccggccg tccgagcgca cgcagcagca aggctgcaac gttggccagc
    11401 ctggcagaca cgccagccat gaagcgggtc aactttcagt tgccggcgga ggatcacacc
    11461 aagctgaaga tgtacgcggt acgccaaggc aagaccatta ccgagctgct atctgaatac
    11521 atcgcgcagc taccagagta aatgagcaaa tgaataaatg agtagatgaa ttttagcggc
    11581 taaaggaggc ggcatggaaa atcaagaaca accaggcacc gacgccgtgg aatgccccat
    11641 gtgtggagga acgggcggtt ggccaggcgt aagcggctgg gttgcctgcc ggccctgcaa
    11701 tggcactgga acccccaagc ccgaggaatc ggcgtgagcg gtcgcaaacc atccggcccg
    11761 gtacaaatcg gcgcggcgct gggtgatgac ctggtggaga agttgaaggc cgcgcaggcc
    11821 gcccagcggc aacgca
//
