LOCUS       sequence-26621-e         778 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 778)
  AUTHORS   Funakoshi M, Hochstrasser M
  TITLE     Small epitope-linker modules for PCR-based C-terminal tagging in 
            Saccharomyces cerevisiae.
  JOURNAL   Yeast. 2009 Feb 25. 26(3):185-192.
  PUBMED    19243080
REFERENCE   2  (bases 1 to 778)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast. 2009
            Feb 25. 26(3):185-192."
COMMENT     Sequence Label: pFA6a-6xGLY-Myc-HIS3MX6
FEATURES             Location/Qualifiers
     source          1..778
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(72..269)
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     CDS             complement(563..577)
                     /codon_start=1
                     /product="tag for protein cleavage using Ni2+ ion (Dang et 
                     al., 2019)"
                     /label=SNAC tag
                     /translation="GSHHW"
     gap             606..617
                     /estimated_length=12
     gap             620..634
                     /estimated_length=15
ORIGIN
        1 agtgganctg atatcatcga tgaattcgag ctcgtttaaa ctggatggcg gcgttagtat
       61 cgaatcgaca gcagtatagc gaccagcatt cacatacgat tgacgcatga tattactttc
      121 tgcgcactta acttcgcatc tgggcagatg atgtcgaggc gaaaaaaaat ataaatcacg
      181 ctaacatttg attaaaatag aacaactaca atataaaaaa actatacaaa tgacaagttc
      241 ttgaaaacaa gaatcttttt attgtcagta cttcacatca aaacaccttt ggttgaggga
      301 acgtcattgg tgccattgct agaaatagct tctcttatgg caacagccaa agccttgaac
      361 gcactctcac ttctgtggtg atcgttgaaa cctctcagac aatcaacatg caaagtaatt
      421 ctggccgcct ccgcgaaact ttccaaaaag tgtggaatca tttcagtgga taaatcacca
      481 atcatctctc tcttcaatcc aaggtcgatt acagcaaatg gtctactaga taaatcgact
      541 acggcacgtg atagcgcctc atccaatggt gcgaacccag taccgaatct ttttacacca
      601 cggacnnnnn nnnnnnnttn nnnnnnnnnn nnnnanggnn ataccgnaat cnttcggtag
      661 tatggtgatc gtcaatgtgc aggtcaccaa tacattcaac aataagagac caaccagagt
      721 gttttgccaa cgcatggatc atatgatcca aaaagccaac acctgtgtga atatcgat
//
