LOCUS       sequence-266257-       10285 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10285)
  AUTHORS   Lee T, Luo L
  TITLE     Mosaic analysis with a repressible cell marker for studies of gene 
            function in neuronal morphogenesis.
  JOURNAL   Neuron. 1999 Mar . 22(3):451-61.
  PUBMED    10197526
REFERENCE   2  (bases 1 to 10285)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Neuron. 
            1999 Mar . 22(3):451-61."
COMMENT     Sequence Label: mCD8-GFP in pUAST
FEATURES             Location/Qualifiers
     source          1..10285
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    62..156
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     promoter        175..413
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     primer_bind     360..381
                     /label=pCasper-hs
                     /note="Drosophila Hsp70 promoter, forward primer"
     CDS             465..1130
                     /codon_start=1
                     /product="mouse lymphocyte antigen CD8 alpha chain"
                     /label=mCD8
                     /translation="MASPLTRFLSLNLLLLGESIILGSGEAKPQAPELRIFPKKMDAEL
                     GQKVDLVCEVLGSVSQGCSWLFQNSSSKLPQPTFVVYMASSHNKITWDEKLNSSKLFSA
                     MRDTNNKYVLTLNKFSKENEGYYFCSVISNSVMYFSSVVPVLQKVNSTTTKPVLRTPSP
                     VHPTGTSQPQRPEDCRPRGSVKGTGLDFACDIYIWAPLAGICVALLLSLIITLICYHSR
                     "
     CDS             1137..1850
                     /codon_start=1
                     /product="green fluorescent protein"
                     /label=GFP
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(1167..1195)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     1790..1810
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     intron          1937..2002
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             2132..2152
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    2424..2558
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2461..2480)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2515..2534
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(2585..2603)
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     CDS             join(3055..3126,3528..3801,3876..4530,4592..4907,5111..5242,
                     5313..5927)
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLRPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     primer_bind     complement(6388..6407)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6507..6529
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(6567..6585)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6653..6757
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6758..6826,6827..7618)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6976..6995)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7789..8377
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8278..8297
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     misc_feature    join(8612..8642,8643..9197)
                     /label=P element 5' end
     misc_feature    join(10053..10083,10084..10285)
                     /label=P element 3' end
                     /note="P element 3' end"
ORIGIN
        1 aagcttggct gcatccaacg cgttgggagc tctccggatc caagcttgca tgcctgcagg
       61 tcggagtact gtcctccgag cggagtactg tcctccgagc ggagtactgt cctccgagcg
      121 gagtactgtc ctccgagcgg agtactgtcc tccgagcgga gactctagcg agcgccggag
      181 tataaataga ggcgcttcgt ctacggagcg acaattcaat tcaaacaagc aaagtgaaca
      241 cgtcgctaag cgaaagctaa gcaaataaac aagcgcagct gaacaagcta aacaatctgc
      301 agtaaagtgc aagttaaagt gaatcaatta aaagtaacca gcaaccaagt aaatcaactg
      361 caactactga aatctgccaa gaagtaatta ttgaatacaa gaagagaact ctgaataggg
      421 aattgggaat tcgttaacag atctgcggcc gcggctcgag caaaatggcc tcaccgttga
      481 cccgctttct gtcgctgaac ctgctgctgc tgggtgagtc gattatcctg gggagtggag
      541 aagctaagcc acaggcaccc gaactccgaa tctttccaaa gaaaatggac gccgaacttg
      601 gtcagaaggt ggacctggta tgtgaagtgt tggggtccgt ttcgcaagga tgctcttggc
      661 tcttccagaa ctccagctcc aaactccccc agcccacctt cgttgtctat atggcttcat
      721 cccacaacaa gataacgtgg gacgagaagc tgaattcgtc gaaactgttt tctgccatga
      781 gggacacgaa taataagtac gttctcaccc tgaacaagtt cagcaaggaa aacgaaggct
      841 actatttctg ctcagtcatc agcaactcgg tgatgtactt cagttctgtc gtgccagtcc
      901 ttcagaaagt gaactctact actaccaagc cagtgctgcg aactccctca cctgtgcacc
      961 ctaccgggac atctcagccc cagagaccag aagattgtcg gccccgtggc tcagtgaagg
     1021 ggaccggatt ggacttcgcc tgtgatattt acatctgggc acccttggcc ggaatctgcg
     1081 tggcccttct gctgtccttg atcatcactc tcatctgcta ccacagccgc ggatccatga
     1141 gtaaaggaga agaacttttc actggagttg tcccaattct tgttgaatta gatggtgatg
     1201 ttaatgggca caaattttct gtcagtggag agggtgaagg tgatgcaaca tacggaaaac
     1261 ttacccttaa atttatttgc actactggaa aactacctgt tccatggcca acacttgtca
     1321 ctactttaac ttatggtgtt caatgctttt caagataccc agatcatatg aaacagcatg
     1381 actttttcaa gagtgccatg cccgaaggtt atgtccagga aagaactata tttttcaaag
     1441 atgacgggaa ctacaagaca cgtgctgaag tcaagtttga aggtgatacc cttgttaata
     1501 gaatcgagtt aaaaggtatt gattttaaag aagatggaaa cattcttgga cacaaattgg
     1561 aatacaacta taactcacac aatgtataca tcatggcaga caaacaaaag aatggaatca
     1621 aagttaactt caaaattaga cacaacattg aagatggaag cgttcaacta gcagaccatt
     1681 atcaacaaaa tactccaatt ggcgatggcc ctgtcctttt accagacaac cattacctgt
     1741 ccacacaatc tgccctttcg aaagatccca acgaaaagag agaccacatg gtccttcttg
     1801 agtttgtaac agctgctggg attacacatg gcatggatga actatacaaa taatctagag
     1861 gatctttgtg aaggaacctt acttctgtgg tgtgacataa ttggacaaac tacctacaga
     1921 gatttaaagc tctaaggtaa atataaaatt tttaagtgta taatgtgtta aactactgat
     1981 tctaattgtt tgtgtatttt agattccaac ctatggaact gatgaatggg agcagtggtg
     2041 gaatgccttt aatgaggaaa acctgttttg ctcagaagaa atgccatcta gtgatgatga
     2101 ggctactgct gactctcaac attctactcc tccaaaaaag aagagaaagg tagaagaccc
     2161 caaggacttt ccttcagaat tgctaagttt tttgagtcat gctgtgttta gtaatagaac
     2221 tcttgcttgc tttgctattt acaccacaaa ggaaaaagct gcactgctat acaagaaaat
     2281 tatggaaaaa tatttgatgt atagtgcctt gactagagat cataatcagc cataccacat
     2341 ttgtagaggt tttacttgct ttaaaaaacc tcccacacct ccccctgaac ctgaaacata
     2401 aaatgaatgc aattgttgtt gttaacttgt ttattgcagc ttataatggt tacaaataaa
     2461 gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt
     2521 tgtccaaact catcaatgta tcttatcatg tctggatcca ctagaaggcc taattctagt
     2581 atgtatgtaa gttaataaaa cccatttttg cggaaagtag ataaaaaaaa catttttttt
     2641 ttttactgca ctggatatca ttgaacttat ctgatcagtt ttaaatttac ttcgatccaa
     2701 gggtatttga tgtaccaggt tctttcgatt acctctcact caaaatgaca ttccactcaa
     2761 agtcagcgct gtttgcctcc ttctctgtcc acagaaatat cgccgtctct ttcgccgctg
     2821 cgtccgctat ctctttcgcc accgtttgta gcgttacgta gcgtcaatgt ccgccttcag
     2881 ttgcattttg tcagcggttt cgtgacgaag ctccaagcgg tttacgccat caattaaaca
     2941 caaagtgctg tgccaaaact cctctcgctt cttatttttg tttgtttttt gagtgattgg
     3001 ggtggtgatt ggttttgggt gggtaagcag gggaaagtgt gaaaaatccc ggcaatgggc
     3061 caagaggatc aggagctatt aattcgcgga ggcagcaaac acccatctgc cgagcatctg
     3121 aacaatgtga gtagtacatg tgcatacatc ttaagttcac ttgatctata ggaactgcga
     3181 ttgcaacatc aaattgtctg cggcgtgaga actgcgaccc acaaaaatcc caaaccgcaa
     3241 ttgcacaaac aaatagtgac acgaaacaga ttattctggt agctgttctc gctatataag
     3301 acaatttttg agatcatatc atgatcaaga catctaaagg cattcatttt cgactatatt
     3361 cttttttaca aaaaatataa caaccagata ttttaagctg atcctagatg cacaaaaaat
     3421 aaataaaagt ataaacctac ttcgtaggat acttcggggt actttttgtt cggggttaga
     3481 tgagcataac gcttgtagtt gatatttgag atcccctatc attgcagggt gacagcggag
     3541 cggcttcgca gagctgcatt aaccagggct tcgggcaggc caaaaactac ggcacgctcc
     3601 ggccacccag tccgccggag gactccggtt cagggagcgg ccaactagcc gagaacctca
     3661 cctatgcctg gcacaatatg gacatctttg gggcggtcaa tcagccgggc tccggatggc
     3721 ggcagctggt caaccggaca cgcggactat tctgcaacga gcgacacata ccggcgccca
     3781 ggaaacattt gctcaagaac ggtgagtttc tattcgcagt cggctgatct gtgtgaaatc
     3841 ttaataaagg gtccaattac caatttgaaa ctcagtttgc ggcgtggcct atccgggcga
     3901 acttttggcc gtgatgggca gttccggtgc cggaaagacg accctgctga atgcccttgc
     3961 ctttcgatcg ccgcagggca tccaagtatc gccatccggg atgcgactgc tcaatggcca
     4021 acctgtggac gccaaggaga tgcaggccag gtgcgcctat gtccagcagg atgacctctt
     4081 tatcggctcc ctaacggcca gggaacacct gattttccaa gccatggtgc ggatgccacg
     4141 acatctgacc tatcggcagc gagtggcccg cgtggatcag gtgatccagg agctttcgct
     4201 cagcaaatgt cagcacacga tcatcggtgt gcccggcagg gtgaaaggtc tgtccggcgg
     4261 agaaaggaag cgtctggcat tcgcctccga ggctctaacc gatccgccgc ttctgatctg
     4321 cgatgagccc acctccggac tggactcctt taccgcccac agcgtcgtcc aggtgctgaa
     4381 gaagctgtcg cagaagggca agaccgtcat cctgaccatt catcagccgt cttccgagct
     4441 gtttgagctc tttgacaaga tccttctgat ggccgagggc agggtagctt tcttgggcac
     4501 tcccagcgaa gccgtcgact tcttttccta gtgagttcga tgtgtttatt aagggtatct
     4561 agtattacat aacatctcaa ctcctatcca gcgtgggtgc ccagtgtcct accaactaca
     4621 atccggcgga cttttacgta caggtgttgg ccgttgtgcc cggacgggag atcgagtccc
     4681 gtgatcggat cgccaagata tgcgacaatt ttgccattag caaagtagcc cgggatatgg
     4741 agcagttgtt ggccaccaaa aatctggaga agccactgga gcagccggag aatgggtaca
     4801 cctacaaggc cacctggttc atgcagttcc gggcggtcct gtggcgatcc tggctgtcgg
     4861 tgctcaagga accactcctc gtaaaagtgc gacttattca gacaacggtg agtggttcca
     4921 gtggaaacaa atgatataac gcttacaatt cttggaaaca aattcgctag attttagata
     4981 gaattgcctg attccacacc cttcttagtt tttttcaatg agatgtatag tttatagttt
     5041 tgcagaagat aaataaattt catttaactc gcgaatatta atgagatgcg agtaacattt
     5101 taatttgcag atggttgcca tcttgattgg cctcatcttt ttgggccaac aactcacgca
     5161 agtgggtgtg atgaatatca acggagccat cttcctcttc ctgaccaaca tgacctttca
     5221 aaacgtcttt gccacgataa atgtaagtca tgtttagaat acatttgcat ttcaataatt
     5281 tactaacttt ctaatgaatc gattcgattt aggtgttcac ctcagagctg ccagttttta
     5341 tgagggaggc ccgaagtcga ctttatcgct gtgacacata ctttctgggc aaaacgattg
     5401 ccgaattgcc gctttttctc acagtgccac tggtcttcac ggcgattgcc tatccgatga
     5461 tcggactgcg ggccggagtg ctgcacttct tcaactgcct ggcgctggtc actctggtgg
     5521 ccaatgtgtc aacgtccttc ggatatctaa tatcctgcgc cagctcctcg acctcgatgg
     5581 cgctgtctgt gggtccgccg gttatcatac cattcctgct ctttggcggc ttcttcttga
     5641 actcgggctc ggtgccagta tacctcaaat ggttgtcgta cctctcatgg ttccgttacg
     5701 ccaacgaggg tctgctgatt aaccaatggg cggacgtgga gccgggcgaa attagctgca
     5761 catcgtcgaa caccacgtgc cccagttcgg gcaaggtcat cctggagacg cttaacttct
     5821 ccgccgccga tctgccgctg gactacgtgg gtctggccat tctcatcgtg agcttccggg
     5881 tgctcgcata tctggctcta agacttcggg cccgacgcaa ggagtagccg acatatatcc
     5941 gaaataactg cttgtttttt ttttttacca ttattaccat cgtgtttact gtttattgcc
     6001 ccctcaaaaa gctaatgtaa ttatatttgt gccaataaaa acaagatatg acctatagaa
     6061 tacaagtatt tccccttcga acatccccac aagtagactt tggatttgtc ttctaaccaa
     6121 aagacttaca cacctgcata ccttacatca aaaactcgtt tatcgctaca taaaacaccg
     6181 ggatatattt tttatataca tacttttcaa atcgcgcgcc ctcttcataa ttcacctcca
     6241 ccacaccacg tttcgtagtt gctctttcgc tgtctcccac ccgctctccg caacacattc
     6301 accttttgtt cgacgacctt ggagcgactg tcgttagttc cgcgcgattc ggtgcggtat
     6361 ttcacaccgc atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca
     6421 gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc
     6481 cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc
     6541 atcaccgaaa cgcgcgagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt
     6601 catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac
     6661 ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc
     6721 ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt
     6781 cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct
     6841 ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga
     6901 tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag
     6961 cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca
     7021 actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga
     7081 aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag
     7141 tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc
     7201 ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa
     7261 tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt
     7321 gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg
     7381 gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt
     7441 tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg
     7501 gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat
     7561 ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact
     7621 gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa
     7681 aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt
     7741 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
     7801 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
     7861 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
     7921 gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt
     7981 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
     8041 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
     8101 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
     8161 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga
     8221 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
     8281 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
     8341 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccttcttct tgaactcggg
     8401 ctcggtgcca gtatacctca aatggttgtc gtacctctca tggttccgtt acgccaacga
     8461 gggtctgctg attaaccaat gggcggacgt ggagccgggc gaaattagct gcacatcgtc
     8521 gaacaccacg tgccccagtt cgggcaaggt catcctggag acgcttaact tctccgccgc
     8581 cgatctgccg ctggactacg tgggtctggc ccatgatgaa ataacataag gtggtcccgt
     8641 cgatagccga agcttaccga agtatacact taaattcagt gcacgtttgc ttgttgagag
     8701 gaaaggttgt gtgcggacga attttttttt gaaaacatta acccttacgt ggaataaaaa
     8761 aaaatgaaat attgcaaatt ttgctgcaaa gctgtgactg gagtaaaatt aattcacgtg
     8821 ccgaagtgtg ctattaagag aaaattgtgg gagcagagcc ttgggtgcag ccttggtgaa
     8881 aactcccaaa tttgtgatac ccactttaat gattcgcagt ggaaggctgc acctgcaaaa
     8941 ggtcagacat ttaaaaggag gcgactcaac gcagatgccg tacctagtaa agtgatagag
     9001 cctgaaccag aaaagataaa agaaggctat accagtggga gtacacaaac agagtaagtt
     9061 tgaatagtaa aaaaaatcat ttatgtaaac aataacgtga ctgtgcgtta ggtcctgttc
     9121 attgtttaat gaaaataaga gcttgaggga aaaaattcgt actttggagt acgaaatgcg
     9181 tcgtttagag cagcagccga attaattcta gttccagtga aatccaagca ttttctaaat
     9241 taaatgtatt cttattatta tagttgttat ttttgatata tataaacaac actattatgc
     9301 ccaccatttt tttgagatgc atctacacaa ggaacaaaca ctggatgtca ctttcagttc
     9361 aaattgtaac gctaatcact ccgaacaggt cacaaaaaat taccttaaaa agtcataata
     9421 ttaaattaga ataaatatag ctgtgaggga aatatataca aatatattgg agcaaataaa
     9481 ttgtacatac aaatatttat tactaatttc tattgagacg aaatgaacca ctcggaacca
     9541 tttgagcgaa ccgaatcgcg cggaactaac gacagtcgct ccaaggtcgt cgaacaaaag
     9601 gtgaatgtgt tgcggagagc gggtgggaga cagcgaaaga gcaactacga aacgtggtgt
     9661 ggtggaggtg aattatgaag agggcgcgcg atttgaaaag tatgtatata aaaaatatat
     9721 cccggtgttt tatgtagcga taaacgagtt tttgatgtaa ggtatgcagg tgtgtaagtc
     9781 ttttggttag aagacaaatc caaagtctac ttgtggggat gttcgaaggg gaaatacttg
     9841 tattctatag gtcatatctt gtttttattg gcacaaatat aattacatta gctttttgag
     9901 ggggcaataa acagtaaaca cgatggtaat aatggtaaaa aaaaaaaaca agcagttatt
     9961 tcggatatat gtcggctact ccttgcgtcg ggcccgaagt cttagagcca gatatgcgag
    10021 cacccggaag ctcacgatga gaatggccag accatgatga aataacataa ggtggtcccg
    10081 tcggcaagag acatccactt aacgtatgct tgcaataagt gcgagtgaaa ggaatagtat
    10141 tctgagtgtc gtattgagtc tgagtgagac agcgatatga ttgttgatta acccttagca
    10201 tgtccgtggg gtttgaatta actcataata ttaattagac gaaattattt ttaaagtttt
    10261 atttttaata atttgcgagt acgca
//
