LOCUS       sequence-266260-        3848 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3848)
  AUTHORS   Schulz TJ, Westermann D, Isken F, Voigt A, Laube B, Thierbach R, 
            Kuhlow D, Zarse K, Schomburg L, Pfeiffer AF, Tschope C, Ristow M
  TITLE     Activation of mitochondrial energy metabolism protects against 
            cardiac failure.
  JOURNAL   Aging (Albany NY). 2010 Nov;2(11):843-53. doi: 
            10.18632/aging.100234.
  PUBMED    21084725
REFERENCE   2  (bases 1 to 3848)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.18632/aging"; 
            journalName: "Aging (Albany NY)"; date: "2010-11"; volume: "2"; 
            issue: "11"; pages: "843-53"
COMMENT     Sequence Label: pDRIVE-CAG-hFX-HA
FEATURES             Location/Qualifiers
     source          1..3848
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1093..1115
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     CDS             1822..1848
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     primer_bind     complement(1901..1920)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      2234..2822
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2723..2742
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        2908..2955
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     promoter        2947..2965
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     enhancer        3418..3721
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        join(3723..3848,1..152)
                     /label=chicken beta-actin promoter
ORIGIN
        1 cgccaggcgg ggcggggcgg ggcgaggggc ggggcggggc gaggcggaga ggtgcggcgg
       61 cagccaatca gagcggcgcg ctccgaaagt ttccttttat ggcgaggcgg cggcggcggc
      121 ggccctataa aaagcgaagc gcgcggcggg cgggagtcgc tgcgcgctgc cttcgccccg
      181 tgccccgctc cgccgccgcc tcgcgccgcc cgccccggct ctgactgacc gcgttactcc
      241 cacaggtgag cgggcgggac ggcccttctc ctccgggctg taattagcgc ttggtttaat
      301 gacggcttgt ttcttttctg tggctgcgtg aaagccttga ggggctccgg gagggccctt
      361 tgtgcggggg gagcggctcg gggggtgcgt gcgtgtgtgt gtgcgtgggg agcgccgcgt
      421 gcggctccgc gctgcccggc ggctgtgagc gctgcgggcg cggcgcgggg ctttgtgcgc
      481 tccgcagtgt gcgcgagggg agcgcggccg ggggcggtgc cccgcggtgc ggggggggct
      541 gcgaggggaa caaaggctgc gtgcggggtg tgtgcgtggg ggggtgagca gggggtgtgg
      601 gcgcgtcggt cgggctgcaa ccccccctgc acccccctcc ccgagttgct gagcacggcc
      661 cggcttcggg tgcggggctc cgtacggggc gtggcgcggg gctcgccgtg ccgggcgggg
      721 ggtggcggca ggtgggggtg ccgggcgggg cggggccgcc tcgggccggg gagggctcgg
      781 gggaggggcg cggcggcccc cggagcgccg gcggctgtcg aggcgcggcg agccgcagcc
      841 attgcctttt atggtaatcg tgcgagaggg cgcagggact tcctttgtcc caaatctgtg
      901 cggagccgaa atctgggagg cgccgccgca ccccctctag cgggcgcggg gcgaagcggt
      961 gcggcgccgg caggaaggaa atgggcgggg agggccttcg tgcgtcgccg cgccgccgtc
     1021 cccttctccc tctccagcct cggggctgtc cgcgggggga cggctgcctt cgggggggac
     1081 ggggcagggc ggggttcggc ttctggcgtg tgaccggcgg ctctagacaa ttgtactaac
     1141 cttcttctct ttcctctcct gacaggttgg tgtacagtag cttccaccat gggatggact
     1201 ctcgggcgcc gcgcagtagc cggcctcctg gcgtcaccca gcccggccca ggcccagacc
     1261 ctcacccggg tcccgcggcc ggcagagttg gccccactct gcggccgccg tggcctgcgc
     1321 accgacatcg atgcgacctg cacgccccgc cgcgcaagtt cgaaccaacg tggcctcaac
     1381 cagatttgga atgtcaaaaa gcagagtgtc tatttgatga atttgaggaa atctggaact
     1441 ttgggccacc caggctctct agatgagacc acctatgaaa gactagcaga ggaaacgctg
     1501 gactctttag cagagttttt tgaagacctt gcagacaagc catacacgtt tgaggactat
     1561 gatgtctcct ttgggagtgg tgtcttaact gtcaaactgg gtggagatct aggaacctat
     1621 gtgatcaaca agcagacgcc aaacaagcaa atctggctat cttctccatc cagtggacct
     1681 aagcgttatg actggactgg gaaaaactgg gtgtactccc acgacggcgt gtccctccat
     1741 gagctgctgg ccgcagagct cactaaagcc ttaaaaacca aactggactt gtcttccttg
     1801 gcctattccg gaaaagatgc ttacccttac gacgtaccag actacgcaga cacgtaccga
     1861 tacatctaga attcgctagc tcgacatgat aagatacatt gatgagtttg gacaaaccac
     1921 aactagaatg cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt
     1981 tgtgaaattt gtgatgctat tgctttattt gtaaccatta taagctgcaa taaacaagtt
     2041 aacaacaaca attgcattca ttttatgttt caggttcagg gggaggtgtg ggaggttttt
     2101 taaagcaagt aaaacctcta caaatgtggt agatccattt aaatgttaat taactagcca
     2161 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga
     2221 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
     2281 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
     2341 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
     2401 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
     2461 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
     2521 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
     2581 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
     2641 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
     2701 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
     2761 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
     2821 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     2881 tgttcttaat taaatttttc aaaagtagtt gacaattaat catcggcata gtatatcggc
     2941 atagtataat acgactcact ataggagggc catcatggcc aagttgacca gtgctgtccc
     3001 agtgctcaca gccagggatg tggctggagc tgttgagttc tggactgaca ggttggggtt
     3061 ctccagagat tttgtggagg atgactttgc aggtgtggtc agagatgatg tcaccctgtt
     3121 catctcagca gtccaggacc aggtggtgcc tgacaacacc ctggcttggg tgtgggtgag
     3181 aggactggat gagctgtatg ctgagtggag tgaggtggtc tccaccaact tcagggatgc
     3241 cagtggccct gccatgacag agattggaga gcagccctgg gggagagagt ttgccctgag
     3301 agacccagca ggcaactgtg tgcactttgt ggcagaggag caggactgag gataagaatt
     3361 gagtttcaga aaagggggcc tgagtggccc cttttttcaa cttaattaac ctgcaggcgt
     3421 tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc gcccattgac
     3481 gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt gacgtcaatg
     3541 ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc atatgccaag
     3601 tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg cccagtacat
     3661 gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg ctattaacat
     3721 ggtcgaggtg agccccacgt tctgcttcac tctccccatc tcccccccct ccccaccccc
     3781 aattttgtat ttatttattt tttaattatt ttgtgcagcg atgggggcgg gggggggggg
     3841 ggggcgcg
//
