LOCUS       sequence-266261-       15896 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  iSTU-CRISPR/Cas9 with StIV2 intron, tRNA structure backbone.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15896)
  AUTHORS   Zhong Z, Liu S, Liu X, Liu B, Tang X, Ren Q, Zhou J, Zheng X, Qi Y, 
            Zhang Y
  TITLE     Intron-Based Single Transcript Unit CRISPR Systems for Plant Genome 
            Editing.
  JOURNAL   Rice (N Y). 2020 Feb 3;13(1):8. doi: 10.1186/s12284-020-0369-8.
  PUBMED    32016614
REFERENCE   2  (bases 1 to 15896)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Rice (N 
            Y)."; date: "2020-02-3"; volume: "13(1):8. doi"; pages: " 
            10.1186/s12284-020-0369-8"
COMMENT     Sequence Label: pGEL078
FEATURES             Location/Qualifiers
     source          1..15896
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        8..2004
                     /label=Ubi promoter
                     /note="maize polyubiquitin gene promoter"
     CDS             complement(3572..3877)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(3674..3693)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     misc_RNA        4204..4279
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     CDS             4806..7190
                     /codon_start=1
                     /product="C-terminal portion of Streptococcus pyogenes Cas9
                     (Zetsche et al., 2015)"
                     /label=Cas9(C)
                     /translation="CFDSVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILED
                     IVLTLTLFEDREMIEERLKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGK
                     TILDFLKSDGFANRNFMQLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKG
                     ILQTVKVVDELVKVMGRHKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQI
                     LKEHPVENTQLQNEKLYLYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDN
                     KVLTRSDKNRGKSDNVPSEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDK
                     AGFIKRQLVETRQITKHVAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQF
                     YKVREINNYHHAHDAYLNAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGK
                     ATAKYFFYSNIMNFFKTEITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMP
                     QVNIVKKTEVQTGGFSKESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKV
                     EKGKSKKLKSVKELLGITIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELEN
                     GRKRMLASAGELQKGNELALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLD
                     EIIEQISEFSKRVILADANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKY
                     FDTTIDRKRYTSTKEVLDATLIHQSITGLYETRIDLSQLGGD"
     CDS             7203..7223
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     terminator      7234..7482
                     /label=HSP terminator
                     /note="efficient transcription terminator from the 
                     Arabidopsis thaliana heat shock protein 18.2 gene (Nagaya 
                     et al., 2010)"
     misc_feature    7503..7527
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             8827..9456
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             9890..10957
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQA
                     AADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKR
                     DRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGR
                     VFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEAL
                     ISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLA
                     RRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILV
                     MRYRNLIEGEASAGS"
     rep_origin      11023..11217
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(11453..11475)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    11561..11701
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     11616..11635
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(11716..11733)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(11887..12475)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11967..11986)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(12562..13356)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    13781..13805
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    13883..14057
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     promoter        complement(15147..15824)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
ORIGIN
        1 ggcgcgcctg cagtgcagcg tgacccggtc gtgcccctct cttgagataa tgagcattgc
       61 atgtctaagt tataaaaaat taccacatat tttttttgtc acacttgttt gaagtgcagt
      121 ttatctatct ttatacatat atttaaactt tactctacga ataatataat ctatagtact
      181 acaataatat cagtgtttta gagaatcata taaatgaaca gttagacatg gtctaaagga
      241 caattgagta ttttgacaac aggactctac agttttatct ttttagtgtg catgtgttct
      301 cctttttttt tgcaaatagc ttcacctata taatacttca tccattttat tagtacatcc
      361 atttagggtt tagggttaat ggtttttata gactaatttt tttagtacat ctattttatt
      421 ctattttagc ctctaaatta agaaaactaa aactctattt tagttttttt atttaataat
      481 ttagatataa aatagaataa aataaagtga ctaaaaatta aacaaatacc ctttaagaaa
      541 ttaaaaaaac taaggaaaca tttttcttgt ttcgagtaga taatgccagc ctgttaaacg
      601 ccgtcgacga gtctaacgga caccaaccag cgaaccagca gcgtcgcgtc gggccaagcg
      661 aagcagacgg cacggcatct ctgtcgctgc ctctggaccc ctctcgagag ttccgctcca
      721 ccgttggact tgctccgctg tcggcatcca gaaattgcgt ggcggagcgg cagacgtgag
      781 ccggcacggc aggcggcctc ctcctcctct cacggcaccg gcagctacgg gggattcctt
      841 tcccaccgct ccttcgcttt cccttcctcg cccgccgtaa taaatagaca ccccctccac
      901 accctctttc cccaacctcg tgttgttcgg agcgcacaca cacacaacca gaactccccc
      961 aaatccaccc gtcggcacct ccgcttcaag gtacgccgct cgtcctcccc ccccccccct
     1021 ctctaccttc tcaagatcgg cgttccggtc catggttagg gcccggtagt tctacttctg
     1081 ttcatgtttg tgttagatcc gtgtttgtgt tagatccgtg ctactagcgt tcgtacacgg
     1141 atgcgacctg tacgtcagac acgttctgat tgctaacttg ccagtgtttc tctttgggga
     1201 atcctgggat ggctctagcc gttccgcaga cgggatcgat ttcatgattt tttttgtttc
     1261 gttgcatagg gtttggtttg cccttttcct ttatttcaat atatgccgtg cacttgtttg
     1321 tcgggtcatc ttttcatgct tttttttgtc ttggttgtga tgatgtggtc tggttgggcg
     1381 gtcgttcaag atcggagtag aattaattct gtttcaaact acctggtgga tttattaatt
     1441 ttggatctgt atgtgtgtgc catacatatt catagttacg aattgaagat gatggatgga
     1501 aatatcgatc taggataggt atacatgttg atgcgggttt tactgatgca tatacagaga
     1561 tgctttttgt tcgcttggtt gtgatgatgt ggtgtggttg ggcggtcgtt cattcgttca
     1621 agatcggagt agaatactgt ttcaaactac ctggtgtatt tattaatttt ggaactgtat
     1681 gtgtgtgtca tacatcttca tagttacgag tttaagatgg atggaaatat cgatctagga
     1741 taggtataca tgttgatgtg ggttttactg atgcatatac atgatggcat atgcagcatc
     1801 tattcatatg ctctaacctt gagtacctat ctattataat aaacaagtat gttttataat
     1861 tattttgatc ttgatatact tggatgatgg catatgcagc agctatatgt ggattttttt
     1921 agccctgcct tcatacgcta tttatttgct tggtactgtt tcttttgtcg atgctcaccc
     1981 tgttgtttgg tgttacttct gcagcctgca ggtagatcgc tcgtcgacat ggataagaag
     2041 tactctatcg gactcgatat cggaactaac tctgtgggat gggctgtgat caccgatgag
     2101 tacaaggtgc catctaagaa gttcaaggtt ctcggaaaca ccgataggca ctctatcaag
     2161 aaaaacctta tcggtgctct cctcttcgat tctggtgaaa ctgctgaggc taccagactc
     2221 aagagaaccg ctagaagaag gtacaccaga agaaagaaca ggatctgcta cctccaagag
     2281 atcttctcta acgagatggc taaagtggat gattcattct tccacaggct cgaagagtca
     2341 ttcctcgtgg aagaagataa gaagcacgag aggcacccta tcttcggaaa catcgttgat
     2401 gaggtggcat accacgagaa gtaccctact atctaccacc tcagaaagaa gctcgttgat
     2461 tctactgata aggctgatct caggctcatc tacctcgctc tcgctcacat gatcaagttc
     2521 agaggacact tcctcatcga gggtgatctc aaccctgata actctgatgt ggataagttg
     2581 ttcatccagc tcgtgcagac ctacaaccag cttttcgaag agaaccctat caacgcttca
     2641 ggtgtggatg ctaaggctat cctctctgct aggctctcta agtcaagaag gcttgagaac
     2701 ctcattgctc agctccctgg tgagaagaag aacggacttt tcggaaactt gatcgctctc
     2761 tctctcggac tcacccctaa cttcaagtct aacttcgatc tcgctgagga tgcaaagctc
     2821 cagctctcaa aggataccta cgatgatgat ctcgataacc tcctcgctca gatcggagat
     2881 cagtacgctg atttgttcct cgctgctaag aacctctctg atgctatcct cctcagtgat
     2941 atcctcagag tgaacaccga gatcaccaag gctccactct cagcttctat gatcaagaga
     3001 tacgatgagc accaccagga tctcacactt ctcaaggctc ttgttagaca gcagctccca
     3061 gagaagtaca aagagatttt cttcgatcag tctaagaacg gatacgctgg ttacatcgat
     3121 ggtggtgcat ctcaagaaga gttctacaag ttcatcaagc ctatcctcga gaagatggat
     3181 ggaaccgagg aactcctcgt gaagctcaat agagaggatc ttctcagaaa gcagaggacc
     3241 ttcgataacg gatctatccc tcatcagatc cacctcggag agttgcacgc tatccttaga
     3301 aggcaagagg atttctaccc attcctcaag gataacaggg aaaagattga gaagattctc
     3361 accttcagaa tcccttacta cgtgggacct ctcgctagag gaaactcaag attcgcttgg
     3421 atgaccagaa agtctgagga aaccatcacc ccttggaact tcgaagaggt aagtttctgc
     3481 ttctaccaac aaagcaccag tggtctagtg gtagaatagt accctgccac ggtacagacc
     3541 cgggttcgat tcccggctgg tgcaggagac cttatattcc ccagaacatc aggttaatgg
     3601 cgtttttgat gtcattttcg cggtggctga gatcagccac ttcttccccg ataacggaaa
     3661 ccggcacact ggccatatcg gtggtcatca tgcgccagct ttcatccccg atatgcacca
     3721 ccgggtaaag ttcacgggag actttatctg acagcagacg tgcactggcc agggggatca
     3781 ccatccgtcg cccgggcgtg tcaataatat cactctgtac atccacaaac agacgataac
     3841 ggctctctct tttataggtg taaaccttaa actgcatttc accagcccct gttctcgtca
     3901 gcaaaagagc cgttcatttc aataaaccgg gcgacctcag ccatcccttc ctgattttcc
     3961 gctttccagc gttcggcacg cagacgacgg gcttcattct gcatggttgt gcttaccaga
     4021 ccggagatat tgacatcata tatgccttga gcaactgata gctgtcgctg tcaactgtca
     4081 ctgtaatacg ctgcttcata gcatacctct ttttgacata cttcgggtat acatatcagt
     4141 atatattctt ataccgcaaa aatcagcgcg caaatacgca tactgttatc tggcttggtc
     4201 tcagttttag agctagaaat agcaagttaa aataaggcta gtccgttatc aacttgaaaa
     4261 agtggcaccg agtcggtgca acaaagcacc agtggtctag tggtagaata gtaccctgcc
     4321 acggtacaga cccgggttcg attcccggct ggtgcatttg atatatatat aataattatc
     4381 attaattagt agtaatataa tatttcaaat atttttttca aaataaaaga atgtagtata
     4441 tagcaattgc ttttctgtag tttataagtg tgtatatttt aatttataac ttttctaata
     4501 tatgaccaaa atttgttgat gtgcaggtgg tggataaggg tgctagtgct cagtctttca
     4561 tcgagaggat gaccaacttc gataagaacc ttccaaacga gaaggtgctc cctaagcact
     4621 ctttgctcta cgagtacttc accgtgtaca acgagttgac caaggttaag tacgtgaccg
     4681 agggaatgag gaagcctgct tttttgtcag gtgagcaaaa gaaggctatc gttgatctct
     4741 tgttcaagac caacagaaag gtgaccgtga agcagctcaa agaggattac ttcaagaaaa
     4801 tcgagtgctt cgattcagtt gagatttctg gtgttgagga taggttcaac gcatctctcg
     4861 gaacctacca cgatctcctc aagatcatta aggataagga tttcttggat aacgaggaaa
     4921 acgaggatat cttggaggat atcgttctta ccctcaccct ctttgaagat agagagatga
     4981 ttgaagaaag gctcaagacc tacgctcatc tcttcgatga taaggtgatg aagcagttga
     5041 agagaagaag atacactggt tggggaaggc tctcaagaaa gctcattaac ggaatcaggg
     5101 ataagcagtc tggaaagaca atccttgatt tcctcaagtc tgatggattc gctaacagaa
     5161 acttcatgca gctcatccac gatgattctc tcacctttaa agaggatatc cagaaggctc
     5221 aggtttcagg acagggtgat agtctccatg agcatatcgc taacctcgct ggatctcctg
     5281 caatcaagaa gggaatcctc cagactgtga aggttgtgga tgagttggtg aaggtgatgg
     5341 gaaggcataa gcctgagaac atcgtgatcg aaatggctag agagaaccag accactcaga
     5401 agggacagaa gaactctagg gaaaggatga agaggatcga ggaaggtatc aaagagcttg
     5461 gatctcagat cctcaaagag caccctgttg agaacactca gctccagaat gagaagctct
     5521 acctctacta cctccagaac ggaagggata tgtatgtgga tcaagagttg gatatcaaca
     5581 ggctctctga ttacgatgtt gatcatatcg tgccacagtc attcttgaag gatgattcta
     5641 tcgataacaa ggtgctcacc aggtctgata agaacagggg taagagtgat aacgtgccaa
     5701 gtgaagaggt tgtgaagaaa atgaagaact attggaggca gctcctcaac gctaagctca
     5761 tcactcagag aaagttcgat aacttgacta aggctgagag gggaggactc tctgaattgg
     5821 ataaggcagg attcatcaag aggcagcttg tggaaaccag gcagatcact aagcacgttg
     5881 cacagatcct cgattctagg atgaacacca agtacgatga gaacgataag ttgatcaggg
     5941 aagtgaaggt tatcaccctc aagtcaaagc tcgtgtctga tttcagaaag gatttccaat
     6001 tctacaaggt gagggaaatc aacaactacc accacgctca cgatgcttac cttaacgctg
     6061 ttgttggaac cgctctcatc aagaagtatc ctaagctcga gtcagagttc gtgtacggtg
     6121 attacaaggt gtacgatgtg aggaagatga tcgctaagtc tgagcaagag atcggaaagg
     6181 ctaccgctaa gtatttcttc tactctaaca tcatgaattt cttcaagacc gagattaccc
     6241 tcgctaacgg tgagatcaga aagaggccac tcatcgagac aaacggtgaa acaggtgaga
     6301 tcgtgtggga taagggaagg gatttcgcta ccgttagaaa ggtgctctct atgccacagg
     6361 tgaacatcgt taagaaaacc gaggtgcaga ccggtggatt ctctaaagag tctatcctcc
     6421 ctaagaggaa ctctgataag ctcattgcta ggaagaagga ttgggaccct aagaaatacg
     6481 gtggtttcga ttctcctacc gtggcttact ctgttctcgt tgtggctaag gttgagaagg
     6541 gaaagagtaa gaagctcaag tctgttaagg aacttctcgg aatcactatc atggaaaggt
     6601 catctttcga gaagaaccca atcgatttcc tcgaggctaa gggatacaaa gaggttaaga
     6661 aggatctcat catcaagctc ccaaagtact cactcttcga actcgagaac ggtagaaaga
     6721 ggatgctcgc ttctgctggt gagcttcaaa agggaaacga gcttgctctc ccatctaagt
     6781 acgttaactt tctttacctc gcttctcact acgagaagtt gaagggatct ccagaagata
     6841 acgagcagaa gcaacttttc gttgagcagc acaagcacta cttggatgag atcatcgagc
     6901 agatctctga gttctctaaa agggtgatcc tcgctgatgc aaacctcgat aaggtgttgt
     6961 ctgcttacaa caagcacaga gataagccta tcagggaaca ggcagagaac atcatccatc
     7021 tcttcaccct taccaacctc ggtgctcctg ctgctttcaa gtacttcgat acaaccatcg
     7081 ataggaagag atacacctct accaaagaag tgctcgatgc taccctcatc catcagtcta
     7141 tcactggact ctacgagact aggatcgatc tctcacagct cggtggtgat tcaagggctg
     7201 atcctaagaa gaagaggaag gtttgactcg agatatgaag atgaagatga aatatttggt
     7261 gtgtcaaata aaaagcttgt gtgcttaagt ttgtgttttt ttcttggctt gttgtgttat
     7321 gaatttgtgg ctttttctaa tattaaatga atgtaagatc acattataat gaataaacaa
     7381 atgtttctat aatccattgt gaatgttttg ttggatctct tctgcagcat ataactactg
     7441 tatgtgctat ggtatggact atggaatatg attaaagata aggagctcaa actatcagtg
     7501 tttgacagga tatattggcg ggtaaaccta agagaaaaga gcgtttatta gaataacgga
     7561 tatttaaaag ggcgtgaaaa ggtttatccg ttcgtccatt tgtatgtgca tgccaaccac
     7621 agggttcccc tcgggatcaa agtactttga tccaacccct ccgctgctat agtgcagtcg
     7681 gcttctgacg ttcagtgcag ccgtcttctg aaaacgacat gtcgcacaag tcctaagtta
     7741 cgcgacaggc tgccgccctg cccttttcct ggcgttttct tgtcgcgtgt tttagtcgca
     7801 taaagtagaa tacttgcgac tagaaccgga gacattacgc catgaacaag agcgccgccg
     7861 ctggcctgct gggctatgcc cgcgtcagca ccgacgacca ggacttgacc aaccaacggg
     7921 ccgaactgca cgcggccggc tgcaccaagc tgttttccga gaagatcacc ggcaccaggc
     7981 gcgaccgccc ggagctggcc aggatgcttg accacctacg ccctggcgac gttgtgacag
     8041 tgaccaggct agaccgcctg gcccgcagca cccgcgacct actggacatt gccgagcgca
     8101 tccaggaggc cggcgcgggc ctgcgtagcc tggcagagcc gtgggccgac accaccacgc
     8161 cggccggccg catggtgttg accgtgttcg ccggcattgc cgagttcgag cgttccctaa
     8221 tcatcgaccg cacccggagc gggcgcgagg ccgccaaggc ccgaggcgtg aagtttggcc
     8281 cccgccctac cctcaccccg gcacagatcg cgcacgcccg cgagctgatc gaccaggaag
     8341 gccgcaccgt gaaagaggcg gctgcactgc ttggcgtgca tcgctcgacc ctgtaccgcg
     8401 cacttgagcg cagcgaggaa gtgacgccca ccgaggccag gcggcgcggt gccttccgtg
     8461 aggacgcatt gaccgaggcc gacgccctgg cggccgccga gaatgaacgc caagaggaac
     8521 aagcatgaaa ccgcaccagg acggccagga cgaaccgttt ttcattaccg aagagatcga
     8581 ggcggagatg atcgcggccg ggtacgtgtt cgagccgccc gcgcacggct caaccgtgcg
     8641 gctgcatgaa atcctggccg gtttgtctga tgccaagctg gcggcctggc cggccagctt
     8701 ggccgctgaa gaaaccgagc gccgccgtct aaaaaggtga tgtgtatttg agtaaaacag
     8761 cttgcgtcat gcggtcgctg cgtatatgat gcgatgagta aataaacaaa tacgcaaggg
     8821 gaacgcatga aggttatcgc tgtacttaac cagaaaggcg ggtcaggcaa gacgaccatc
     8881 gcaacccatc tagcccgcgc cctgcaactc gccggggccg atgttctgtt agtcgattcc
     8941 gatccccagg gcagtgcccg cgattgggcg gccgtgcggg aagatcaacc gctaaccgtt
     9001 gtcggcatcg accgcccgac gattgaccgc gacgtgaagg ccatcggccg gcgcgacttc
     9061 gtagtgatcg acggagcgcc ccaggcggcg gacttggctg tgtccgcgat caaggcagcc
     9121 gacttcgtgc tgattccggt gcagccaagc ccttacgaca tatgggccac cgccgacctg
     9181 gtggagctgg ttaagcagcg cattgaggtc acggatggaa ggctacaagc ggcctttgtc
     9241 gtgtcgcggg cgatcaaagg cacgcgcatc ggcggtgagg ttgccgaggc gctggccggg
     9301 tacgagctgc ccattcttga gtcccgtatc acgcagcgcg tgagctaccc aggcactgcc
     9361 gccgccggca caaccgttct tgaatcagaa cccgagggcg acgctgcccg cgaggtccag
     9421 gcgctggccg ctgaaattaa atcaaaactc atttgagtta atgaggtaaa gagaaaatga
     9481 gcaaaagcac aaacacgcta agtgccggcc gtccgagcgc acgcagcagc aaggctgcaa
     9541 cgttggccag cctggcagac acgccagcca tgaagcgggt caactttcag ttgccggcgg
     9601 aggatcacac caagctgaag atgtacgcgg tacgccaagg caagaccatt accgagctgc
     9661 tatctgaata catcgcgcag ctaccagagt aaatgagcaa atgaataaat gagtagatga
     9721 attttagcgg ctaaaggagg cggcatggaa aatcaagaac aaccaggcac cgacgccgtg
     9781 gaatgcccca tgtgtggagg aacgggcggt tggccaggcg taagcggctg ggttgtctgc
     9841 cggccctgca atggcactgg aacccccaag cccgaggaat cggcgtgacg gtcgcaaacc
     9901 atccggcccg gtacaaatcg gcgcggcgct gggtgatgac ctggtggaga agttgaaggc
     9961 cgcgcaggcc gcccagcggc aacgcatcga ggcagaagca cgccccggtg aatcgtggca
    10021 agcggccgct gatcgaatcc gcaaagaatc ccggcaaccg ccggcagccg gtgcgccgtc
    10081 gattaggaag ccgcccaagg gcgacgagca accagatttt ttcgttccga tgctctatga
    10141 cgtgggcacc cgcgatagtc gcagcatcat ggacgtggcc gttttccgtc tgtcgaagcg
    10201 tgaccgacga gctggcgagg tgatccgcta cgagcttcca gacgggcacg tagaggtttc
    10261 cgcagggccg gccggcatgg ccagtgtgtg ggattacgac ctggtactga tggcggtttc
    10321 ccatctaacc gaatccatga accgataccg ggaagggaag ggagacaagc ccggccgcgt
    10381 gttccgtcca cacgttgcgg acgtactcaa gttctgccgg cgagccgatg gcggaaagca
    10441 gaaagacgac ctggtagaaa cctgcattcg gttaaacacc acgcacgttg ccatgcagcg
    10501 tacgaagaag gccaagaacg gccgcctggt gacggtatcc gagggtgaag ccttgattag
    10561 ccgctacaag atcgtaaaga gcgaaaccgg gcggccggag tacatcgaga tcgagctagc
    10621 tgattggatg taccgcgaga tcacagaagg caagaacccg gacgtgctga cggttcaccc
    10681 cgattacttt ttgatcgatc ccggcatcgg ccgttttctc taccgcctgg cacgccgcgc
    10741 cgcaggcaag gcagaagcca gatggttgtt caagacgatc tacgaacgca gtggcagcgc
    10801 cggagagttc aagaagttct gtttcaccgt gcgcaagctg atcgggtcaa atgacctgcc
    10861 ggagtacgat ttgaaggagg aggcggggca ggctggcccg atcctagtca tgcgctaccg
    10921 caacctgatc gagggcgaag catccgccgg ttcctaatgt acggagcaga tgctagggca
    10981 aattgcccta gcaggggaaa aaggtcgaaa aggcctcttt cctgtggata gcacgtacat
    11041 tgggaaccca aagccgtaca ttgggaaccg gaacccgtac attgggaacc caaagccgta
    11101 cattgggaac cggtcacaca tgtaagtgac tgatataaaa gagaaaaaag gcgatttttc
    11161 cgcctaaaac tctttaaaac ttattaaaac tcttaaaacc cgcctggcct gtgcataact
    11221 gtctggccag cgcacagccg aagagctgca aaaagcgcct acccttcggt cgctgcgctc
    11281 cctacgcccc gccgcttcgc gtcggcctat cgcggccgct ggccgctcaa aaatggctgg
    11341 cctacggcca ggcaatctac cagggcgcgg acaagccgcg ccgtcgccac tcgaccgccg
    11401 gcgcccacat caaggcaccc tgcctcgcgc gtttcggtga tgacggtgaa aacctctgac
    11461 acatgcagct cccggaaacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag
    11521 cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg cgcagccatg acccagtcac
    11581 gtagcgatag cggagtgtat actggcttaa ctatgcggca tcagagcaga ttgtactgag
    11641 agtgcaccat atgcggtgtg aaataccgca cagatgcgta aggagaaaat accgcatcag
    11701 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
    11761 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
    11821 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
    11881 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
    11941 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
    12001 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
    12061 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
    12121 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
    12181 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
    12241 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
    12301 gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc
    12361 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
    12421 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
    12481 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
    12541 tttggtcatg cattctaggt actaaaacaa ttcatccagt aaaatataat attttatttt
    12601 ctcccaatca ggcttgatcc ccagtaagtc aaaaaatagc tcgacatact gttcttcccc
    12661 gatatcctcc ctgatcgacc ggacgcagaa ggcaatgtca taccacttgt ccgccctgcc
    12721 gcttctccca agatcaataa agccacttac tttgccatct ttcacaaaga tgttgctgtc
    12781 tcccaggtcg ccgtgggaaa agacaagttc ctcttcgggc ttttccgtct ttaaaaaatc
    12841 atacagctcg cgcggatctt taaatggagt gtcttcttcc cagttttcgc aatccacatc
    12901 ggccagatcg ttattcagta agtaatccaa ttcggctaag cggctgtcta agctattcgt
    12961 atagggacaa tccgatatgt cgatggagtg aaagagcctg atgcactccg catacagctc
    13021 gataatcttt tcagggcttt gttcatcttc atactcttcc gagcaaagga cgccatcggc
    13081 ctcactcatg agcagattgc tccagccatc atgccgttca aagtgcagga cctttggaac
    13141 aggcagcttt ccttccagcc atagcatcat gtccttttcc cgttccacat cataggtggt
    13201 ccctttatac cggctgtccg tcatttttaa atataggttt tcattttctc ccaccagctt
    13261 atatacctta gcaggagaca ttccttccgt atcttttacg cagcggtatt tttcgatcag
    13321 ttttttcaat tccggtgata ttctcatttt agccatttat tatttccttc ctcttttcta
    13381 cagtatttaa agatacccca agaagctaat tataacaaga cgaactccaa ttcactgttc
    13441 cttgcattct aaaaccttaa ataccagaaa acagcttttt caaagttgtt ttcaaagttg
    13501 gcgtataaca tagtatcgac ggagccgatt ttgaaaccgc ggtgatcaca ggcagcaacg
    13561 ctctgtcatc gttacaatca acatgctacc ctccgcgaga tcatccgtgt ttcaaacccg
    13621 gcagcttagt tgccgttctt ccgaatagca tcggtaacat gagcaaagtc tgccgcctta
    13681 caacggctct cccgctgacg ccgtcccgga ctgatgggct gcctgtatcg agtggtgatt
    13741 ttgtgccgag ctgccggtcg gggagctgtt ggctggctgg tggcaggata tattgtggtg
    13801 taaacaaatt gacgcttaga caacttaata acacattgcg gacgttttta atgtactgaa
    13861 ttaacgccga attaattcgg gggatctgga ttttagtact ggattttggt tttaggaatt
    13921 agaaatttta ttgatagaag tattttacaa atacaaatac atactaaggg tttcttatat
    13981 gctcaacaca tgagcgaaac cctataggaa ccctaattcc cttatctggg aactactcac
    14041 acattattat ggagaaaata gagcttgtcg atcgttaatt aatcacctcc aagatcccgg
    14101 acgagtgctg gggcgtcggt ttccactatc ggcgagtact tctacacagc catcggtcca
    14161 gacggccgcg cttctgcggg cgatttgtgt acgcccgaca gtcccggctc cggatcggac
    14221 gattgcgtcg catcgaccct gcgcccaagc tgcatcatcg aaattgccgt caaccaagct
    14281 ctgatagagt tggtcaagac caatgcggag catatacgcc cggagccgcg gcgatcctgc
    14341 aagctccgga tgcctccgct cgaagtagcg cgtctgctgc tccatacaag ccaaccacgg
    14401 cctccagaag aagatgttgg cgacctcgta ttgggaatcc ccgaacatcg cctcgctcca
    14461 gtcaatgacc gctgttatgc ggccattgtc cgtcaggaca ttgttggagc cgaaatccgc
    14521 gtgcacgagg tgccggactt cggggcagtc ctcggcccaa agcatcagct catcgagagc
    14581 ctgcgcgacg gacgcactga cggtgtcgtc catcacagtt tgccagtgat acacatgggg
    14641 atcagcaatc gcgcatatga aatcacgcca tgtagtgtat tgaccgattc cttgcggtcc
    14701 gaatgggccg aacccgctcg tctggctaag atcggccgca gcgatcgcat ccatggcctc
    14761 cgcgaccggc tgcagaacag cgggcagttc ggtttcaggc aggtcttgca acgtgacacc
    14821 ctgtgcacgg cgggagatgc aataggtcag gctctcgctg aattccccaa tgtcaagcac
    14881 ttccggaatc gggagcgcgg ccgatgcaaa gtgccgataa acataacgat ctttgtagaa
    14941 accatcggcg cagctattta cccgcaggac atatccacgc cctcctacat cgaagctgaa
    15001 agcacgagat tcttcgccct ccgagagctg catcaggtcg gacacgctgt cgaacttttc
    15061 gatcagaaac ttctcgacag acgtcgcggt gagttcaggc tttttcatcc taggagagat
    15121 agatttgtag agagagactg gtgatttcag cgtgtcctct ccaaatgaaa tgaacttcct
    15181 tatatagagg aaggtcttgc gaaggatagt gggattgtgc gtcatccctt acgtcagtgg
    15241 agatatcaca tcaatccact tgctttgaag acgtggttgg aacgtcttct ttttccacga
    15301 tgctcctcgt gggtgggggt ccatctttgg gaccactgtc ggcagaggca tcttgaacga
    15361 tagcctttcc tttatcgcaa tgatggcatt tgtaggtgcc accttccttt tctactgtcc
    15421 ttttgatgaa gtgacagata gctgggcaat ggaatccgag gaggtttccc gatattaccc
    15481 tttgttgaaa agtctcaata gccctttggt cttctgagac tgtatctttg atattcttgg
    15541 agtagacgag agtgtcgtgc tccaccatgt tatcacatca atccacttgc tttgaagacg
    15601 tggttggaac gtcttctttt tccacgatgc tcctcgtggg tgggggtcca tctttgggac
    15661 cactgtcggc agaggcatct tgaacgatag cctttccttt atcgcaatga tggcatttgt
    15721 aggtgccacc ttccttttct actgtccttt tgatgaagtg acagatagct gggcaatgga
    15781 atccgaggag gtttcccgat attacccttt gttgaaaagt ctcaatagcc ctttggtctt
    15841 ctgagactgt atctttgata ttcttggagt agacgagagt gtcgtgctcc accatg
//
