LOCUS       sequence-266263-       15218 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  iSTU-CRISPR/Cas9 with OsCDPK2 intron, NU structure backbone.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15218)
  AUTHORS   Zhong Z, Liu S, Liu X, Liu B, Tang X, Ren Q, Zhou J, Zheng X, Qi Y, 
            Zhang Y
  TITLE     Intron-Based Single Transcript Unit CRISPR Systems for Plant Genome 
            Editing.
  JOURNAL   Rice (N Y). 2020 Feb 3;13(1):8. doi: 10.1186/s12284-020-0369-8.
  PUBMED    32016614
REFERENCE   2  (bases 1 to 15218)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Rice (N 
            Y)."; date: "2020-02-3"; volume: "13(1):8. doi"; pages: " 
            10.1186/s12284-020-0369-8"
COMMENT     Sequence Label: pGEL080
FEATURES             Location/Qualifiers
     source          1..15218
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        8..2004
                     /label=Ubi promoter
                     /note="maize polyubiquitin gene promoter"
     misc_RNA        3504..3579
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     CDS             4128..6512
                     /codon_start=1
                     /product="C-terminal portion of Streptococcus pyogenes Cas9
                     (Zetsche et al., 2015)"
                     /label=Cas9(C)
                     /translation="CFDSVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILED
                     IVLTLTLFEDREMIEERLKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGK
                     TILDFLKSDGFANRNFMQLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKG
                     ILQTVKVVDELVKVMGRHKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQI
                     LKEHPVENTQLQNEKLYLYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDN
                     KVLTRSDKNRGKSDNVPSEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDK
                     AGFIKRQLVETRQITKHVAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQF
                     YKVREINNYHHAHDAYLNAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGK
                     ATAKYFFYSNIMNFFKTEITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMP
                     QVNIVKKTEVQTGGFSKESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKV
                     EKGKSKKLKSVKELLGITIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELEN
                     GRKRMLASAGELQKGNELALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLD
                     EIIEQISEFSKRVILADANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKY
                     FDTTIDRKRYTSTKEVLDATLIHQSITGLYETRIDLSQLGGD"
     CDS             6525..6545
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     terminator      6556..6804
                     /label=HSP terminator
                     /note="efficient transcription terminator from the 
                     Arabidopsis thaliana heat shock protein 18.2 gene (Nagaya 
                     et al., 2010)"
     misc_feature    6825..6849
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             8149..8778
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             9212..10279
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQA
                     AADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKR
                     DRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGR
                     VFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEAL
                     ISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLA
                     RRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILV
                     MRYRNLIEGEASAGS"
     rep_origin      10345..10539
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(10775..10797)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    10883..11023
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     10938..10957
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(11038..11055)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(11209..11797)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11289..11308)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(11884..12678)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    13103..13127
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    13205..13379
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     promoter        complement(14469..15146)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
ORIGIN
        1 ggcgcgcctg cagtgcagcg tgacccggtc gtgcccctct cttgagataa tgagcattgc
       61 atgtctaagt tataaaaaat taccacatat tttttttgtc acacttgttt gaagtgcagt
      121 ttatctatct ttatacatat atttaaactt tactctacga ataatataat ctatagtact
      181 acaataatat cagtgtttta gagaatcata taaatgaaca gttagacatg gtctaaagga
      241 caattgagta ttttgacaac aggactctac agttttatct ttttagtgtg catgtgttct
      301 cctttttttt tgcaaatagc ttcacctata taatacttca tccattttat tagtacatcc
      361 atttagggtt tagggttaat ggtttttata gactaatttt tttagtacat ctattttatt
      421 ctattttagc ctctaaatta agaaaactaa aactctattt tagttttttt atttaataat
      481 ttagatataa aatagaataa aataaagtga ctaaaaatta aacaaatacc ctttaagaaa
      541 ttaaaaaaac taaggaaaca tttttcttgt ttcgagtaga taatgccagc ctgttaaacg
      601 ccgtcgacga gtctaacgga caccaaccag cgaaccagca gcgtcgcgtc gggccaagcg
      661 aagcagacgg cacggcatct ctgtcgctgc ctctggaccc ctctcgagag ttccgctcca
      721 ccgttggact tgctccgctg tcggcatcca gaaattgcgt ggcggagcgg cagacgtgag
      781 ccggcacggc aggcggcctc ctcctcctct cacggcaccg gcagctacgg gggattcctt
      841 tcccaccgct ccttcgcttt cccttcctcg cccgccgtaa taaatagaca ccccctccac
      901 accctctttc cccaacctcg tgttgttcgg agcgcacaca cacacaacca gaactccccc
      961 aaatccaccc gtcggcacct ccgcttcaag gtacgccgct cgtcctcccc ccccccccct
     1021 ctctaccttc tcaagatcgg cgttccggtc catggttagg gcccggtagt tctacttctg
     1081 ttcatgtttg tgttagatcc gtgtttgtgt tagatccgtg ctactagcgt tcgtacacgg
     1141 atgcgacctg tacgtcagac acgttctgat tgctaacttg ccagtgtttc tctttgggga
     1201 atcctgggat ggctctagcc gttccgcaga cgggatcgat ttcatgattt tttttgtttc
     1261 gttgcatagg gtttggtttg cccttttcct ttatttcaat atatgccgtg cacttgtttg
     1321 tcgggtcatc ttttcatgct tttttttgtc ttggttgtga tgatgtggtc tggttgggcg
     1381 gtcgttcaag atcggagtag aattaattct gtttcaaact acctggtgga tttattaatt
     1441 ttggatctgt atgtgtgtgc catacatatt catagttacg aattgaagat gatggatgga
     1501 aatatcgatc taggataggt atacatgttg atgcgggttt tactgatgca tatacagaga
     1561 tgctttttgt tcgcttggtt gtgatgatgt ggtgtggttg ggcggtcgtt cattcgttca
     1621 agatcggagt agaatactgt ttcaaactac ctggtgtatt tattaatttt ggaactgtat
     1681 gtgtgtgtca tacatcttca tagttacgag tttaagatgg atggaaatat cgatctagga
     1741 taggtataca tgttgatgtg ggttttactg atgcatatac atgatggcat atgcagcatc
     1801 tattcatatg ctctaacctt gagtacctat ctattataat aaacaagtat gttttataat
     1861 tattttgatc ttgatatact tggatgatgg catatgcagc agctatatgt ggattttttt
     1921 agccctgcct tcatacgcta tttatttgct tggtactgtt tcttttgtcg atgctcaccc
     1981 tgttgtttgg tgttacttct gcagcctgca ggtagatcgc tcgtcgacat ggataagaag
     2041 tactctatcg gactcgatat cggaactaac tctgtgggat gggctgtgat caccgatgag
     2101 tacaaggtgc catctaagaa gttcaaggtt ctcggaaaca ccgataggca ctctatcaag
     2161 aaaaacctta tcggtgctct cctcttcgat tctggtgaaa ctgctgaggc taccagactc
     2221 aagagaaccg ctagaagaag gtacaccaga agaaagaaca ggatctgcta cctccaagag
     2281 atcttctcta acgagatggc taaagtggat gattcattct tccacaggct cgaagagtca
     2341 ttcctcgtgg aagaagataa gaagcacgag aggcacccta tcttcggaaa catcgttgat
     2401 gaggtggcat accacgagaa gtaccctact atctaccacc tcagaaagaa gctcgttgat
     2461 tctactgata aggctgatct caggctcatc tacctcgctc tcgctcacat gatcaagttc
     2521 agaggacact tcctcatcga gggtgatctc aaccctgata actctgatgt ggataagttg
     2581 ttcatccagc tcgtgcagac ctacaaccag cttttcgaag agaaccctat caacgcttca
     2641 ggtgtggatg ctaaggctat cctctctgct aggctctcta agtcaagaag gcttgagaac
     2701 ctcattgctc agctccctgg tgagaagaag aacggacttt tcggaaactt gatcgctctc
     2761 tctctcggac tcacccctaa cttcaagtct aacttcgatc tcgctgagga tgcaaagctc
     2821 cagctctcaa aggataccta cgatgatgat ctcgataacc tcctcgctca gatcggagat
     2881 cagtacgctg atttgttcct cgctgctaag aacctctctg atgctatcct cctcagtgat
     2941 atcctcagag tgaacaccga gatcaccaag gctccactct cagcttctat gatcaagaga
     3001 tacgatgagc accaccagga tctcacactt ctcaaggctc ttgttagaca gcagctccca
     3061 gagaagtaca aagagatttt cttcgatcag tctaagaacg gatacgctgg ttacatcgat
     3121 ggtggtgcat ctcaagaaga gttctacaag ttcatcaagc ctatcctcga gaagatggat
     3181 ggaaccgagg aactcctcgt gaagctcaat agagaggatc ttctcagaaa gcagaggacc
     3241 ttcgataacg gatctatccc tcatcagatc cacctcggag agttgcacgc tatccttaga
     3301 aggcaagagg atttctaccc attcctcaag gataacaggg aaaagattga gaagattctc
     3361 accttcagaa tcccttacta cgtgggacct ctcgctagag gaaactcaag attcgcttgg
     3421 atgaccagaa agtctgagga aaccatcacc ccttggaact tcgaagaggt caatatgctg
     3481 tcaaaggcag gagaccggtc tcagttttag agctagaaat agcaagttaa aataaggcta
     3541 gtccgttatc aacttgaaaa agtggcaccg agtcggtgct ttcttttctt ttccttttcg
     3601 ttgcttgttt tccccttctg atgttgtgtg ttgagtttat tgtttggttt gctggtagat
     3661 ccagtatttg gatcattttt cctttttttt ttctttcatt gcttctgcgg agggatgagt
     3721 ttgtgcatga atcttttgtt ggttggattc gcttagtttt cacactagtc ctttgattca
     3781 gcgcaaatct ttgttaattg gctcgcttaa ttctgacata aagtgttcct tcttggttct
     3841 gcttgcaggt ggtggataag ggtgctagtg ctcagtcttt catcgagagg atgaccaact
     3901 tcgataagaa ccttccaaac gagaaggtgc tccctaagca ctctttgctc tacgagtact
     3961 tcaccgtgta caacgagttg accaaggtta agtacgtgac cgagggaatg aggaagcctg
     4021 cttttttgtc aggtgagcaa aagaaggcta tcgttgatct cttgttcaag accaacagaa
     4081 aggtgaccgt gaagcagctc aaagaggatt acttcaagaa aatcgagtgc ttcgattcag
     4141 ttgagatttc tggtgttgag gataggttca acgcatctct cggaacctac cacgatctcc
     4201 tcaagatcat taaggataag gatttcttgg ataacgagga aaacgaggat atcttggagg
     4261 atatcgttct taccctcacc ctctttgaag atagagagat gattgaagaa aggctcaaga
     4321 cctacgctca tctcttcgat gataaggtga tgaagcagtt gaagagaaga agatacactg
     4381 gttggggaag gctctcaaga aagctcatta acggaatcag ggataagcag tctggaaaga
     4441 caatccttga tttcctcaag tctgatggat tcgctaacag aaacttcatg cagctcatcc
     4501 acgatgattc tctcaccttt aaagaggata tccagaaggc tcaggtttca ggacagggtg
     4561 atagtctcca tgagcatatc gctaacctcg ctggatctcc tgcaatcaag aagggaatcc
     4621 tccagactgt gaaggttgtg gatgagttgg tgaaggtgat gggaaggcat aagcctgaga
     4681 acatcgtgat cgaaatggct agagagaacc agaccactca gaagggacag aagaactcta
     4741 gggaaaggat gaagaggatc gaggaaggta tcaaagagct tggatctcag atcctcaaag
     4801 agcaccctgt tgagaacact cagctccaga atgagaagct ctacctctac tacctccaga
     4861 acggaaggga tatgtatgtg gatcaagagt tggatatcaa caggctctct gattacgatg
     4921 ttgatcatat cgtgccacag tcattcttga aggatgattc tatcgataac aaggtgctca
     4981 ccaggtctga taagaacagg ggtaagagtg ataacgtgcc aagtgaagag gttgtgaaga
     5041 aaatgaagaa ctattggagg cagctcctca acgctaagct catcactcag agaaagttcg
     5101 ataacttgac taaggctgag aggggaggac tctctgaatt ggataaggca ggattcatca
     5161 agaggcagct tgtggaaacc aggcagatca ctaagcacgt tgcacagatc ctcgattcta
     5221 ggatgaacac caagtacgat gagaacgata agttgatcag ggaagtgaag gttatcaccc
     5281 tcaagtcaaa gctcgtgtct gatttcagaa aggatttcca attctacaag gtgagggaaa
     5341 tcaacaacta ccaccacgct cacgatgctt accttaacgc tgttgttgga accgctctca
     5401 tcaagaagta tcctaagctc gagtcagagt tcgtgtacgg tgattacaag gtgtacgatg
     5461 tgaggaagat gatcgctaag tctgagcaag agatcggaaa ggctaccgct aagtatttct
     5521 tctactctaa catcatgaat ttcttcaaga ccgagattac cctcgctaac ggtgagatca
     5581 gaaagaggcc actcatcgag acaaacggtg aaacaggtga gatcgtgtgg gataagggaa
     5641 gggatttcgc taccgttaga aaggtgctct ctatgccaca ggtgaacatc gttaagaaaa
     5701 ccgaggtgca gaccggtgga ttctctaaag agtctatcct ccctaagagg aactctgata
     5761 agctcattgc taggaagaag gattgggacc ctaagaaata cggtggtttc gattctccta
     5821 ccgtggctta ctctgttctc gttgtggcta aggttgagaa gggaaagagt aagaagctca
     5881 agtctgttaa ggaacttctc ggaatcacta tcatggaaag gtcatctttc gagaagaacc
     5941 caatcgattt cctcgaggct aagggataca aagaggttaa gaaggatctc atcatcaagc
     6001 tcccaaagta ctcactcttc gaactcgaga acggtagaaa gaggatgctc gcttctgctg
     6061 gtgagcttca aaagggaaac gagcttgctc tcccatctaa gtacgttaac tttctttacc
     6121 tcgcttctca ctacgagaag ttgaagggat ctccagaaga taacgagcag aagcaacttt
     6181 tcgttgagca gcacaagcac tacttggatg agatcatcga gcagatctct gagttctcta
     6241 aaagggtgat cctcgctgat gcaaacctcg ataaggtgtt gtctgcttac aacaagcaca
     6301 gagataagcc tatcagggaa caggcagaga acatcatcca tctcttcacc cttaccaacc
     6361 tcggtgctcc tgctgctttc aagtacttcg atacaaccat cgataggaag agatacacct
     6421 ctaccaaaga agtgctcgat gctaccctca tccatcagtc tatcactgga ctctacgaga
     6481 ctaggatcga tctctcacag ctcggtggtg attcaagggc tgatcctaag aagaagagga
     6541 aggtttgact cgagatatga agatgaagat gaaatatttg gtgtgtcaaa taaaaagctt
     6601 gtgtgcttaa gtttgtgttt ttttcttggc ttgttgtgtt atgaatttgt ggctttttct
     6661 aatattaaat gaatgtaaga tcacattata atgaataaac aaatgtttct ataatccatt
     6721 gtgaatgttt tgttggatct cttctgcagc atataactac tgtatgtgct atggtatgga
     6781 ctatggaata tgattaaaga taaggagctc aaactatcag tgtttgacag gatatattgg
     6841 cgggtaaacc taagagaaaa gagcgtttat tagaataacg gatatttaaa agggcgtgaa
     6901 aaggtttatc cgttcgtcca tttgtatgtg catgccaacc acagggttcc cctcgggatc
     6961 aaagtacttt gatccaaccc ctccgctgct atagtgcagt cggcttctga cgttcagtgc
     7021 agccgtcttc tgaaaacgac atgtcgcaca agtcctaagt tacgcgacag gctgccgccc
     7081 tgcccttttc ctggcgtttt cttgtcgcgt gttttagtcg cataaagtag aatacttgcg
     7141 actagaaccg gagacattac gccatgaaca agagcgccgc cgctggcctg ctgggctatg
     7201 cccgcgtcag caccgacgac caggacttga ccaaccaacg ggccgaactg cacgcggccg
     7261 gctgcaccaa gctgttttcc gagaagatca ccggcaccag gcgcgaccgc ccggagctgg
     7321 ccaggatgct tgaccaccta cgccctggcg acgttgtgac agtgaccagg ctagaccgcc
     7381 tggcccgcag cacccgcgac ctactggaca ttgccgagcg catccaggag gccggcgcgg
     7441 gcctgcgtag cctggcagag ccgtgggccg acaccaccac gccggccggc cgcatggtgt
     7501 tgaccgtgtt cgccggcatt gccgagttcg agcgttccct aatcatcgac cgcacccgga
     7561 gcgggcgcga ggccgccaag gcccgaggcg tgaagtttgg cccccgccct accctcaccc
     7621 cggcacagat cgcgcacgcc cgcgagctga tcgaccagga aggccgcacc gtgaaagagg
     7681 cggctgcact gcttggcgtg catcgctcga ccctgtaccg cgcacttgag cgcagcgagg
     7741 aagtgacgcc caccgaggcc aggcggcgcg gtgccttccg tgaggacgca ttgaccgagg
     7801 ccgacgccct ggcggccgcc gagaatgaac gccaagagga acaagcatga aaccgcacca
     7861 ggacggccag gacgaaccgt ttttcattac cgaagagatc gaggcggaga tgatcgcggc
     7921 cgggtacgtg ttcgagccgc ccgcgcacgg ctcaaccgtg cggctgcatg aaatcctggc
     7981 cggtttgtct gatgccaagc tggcggcctg gccggccagc ttggccgctg aagaaaccga
     8041 gcgccgccgt ctaaaaaggt gatgtgtatt tgagtaaaac agcttgcgtc atgcggtcgc
     8101 tgcgtatatg atgcgatgag taaataaaca aatacgcaag gggaacgcat gaaggttatc
     8161 gctgtactta accagaaagg cgggtcaggc aagacgacca tcgcaaccca tctagcccgc
     8221 gccctgcaac tcgccggggc cgatgttctg ttagtcgatt ccgatcccca gggcagtgcc
     8281 cgcgattggg cggccgtgcg ggaagatcaa ccgctaaccg ttgtcggcat cgaccgcccg
     8341 acgattgacc gcgacgtgaa ggccatcggc cggcgcgact tcgtagtgat cgacggagcg
     8401 ccccaggcgg cggacttggc tgtgtccgcg atcaaggcag ccgacttcgt gctgattccg
     8461 gtgcagccaa gcccttacga catatgggcc accgccgacc tggtggagct ggttaagcag
     8521 cgcattgagg tcacggatgg aaggctacaa gcggcctttg tcgtgtcgcg ggcgatcaaa
     8581 ggcacgcgca tcggcggtga ggttgccgag gcgctggccg ggtacgagct gcccattctt
     8641 gagtcccgta tcacgcagcg cgtgagctac ccaggcactg ccgccgccgg cacaaccgtt
     8701 cttgaatcag aacccgaggg cgacgctgcc cgcgaggtcc aggcgctggc cgctgaaatt
     8761 aaatcaaaac tcatttgagt taatgaggta aagagaaaat gagcaaaagc acaaacacgc
     8821 taagtgccgg ccgtccgagc gcacgcagca gcaaggctgc aacgttggcc agcctggcag
     8881 acacgccagc catgaagcgg gtcaactttc agttgccggc ggaggatcac accaagctga
     8941 agatgtacgc ggtacgccaa ggcaagacca ttaccgagct gctatctgaa tacatcgcgc
     9001 agctaccaga gtaaatgagc aaatgaataa atgagtagat gaattttagc ggctaaagga
     9061 ggcggcatgg aaaatcaaga acaaccaggc accgacgccg tggaatgccc catgtgtgga
     9121 ggaacgggcg gttggccagg cgtaagcggc tgggttgtct gccggccctg caatggcact
     9181 ggaaccccca agcccgagga atcggcgtga cggtcgcaaa ccatccggcc cggtacaaat
     9241 cggcgcggcg ctgggtgatg acctggtgga gaagttgaag gccgcgcagg ccgcccagcg
     9301 gcaacgcatc gaggcagaag cacgccccgg tgaatcgtgg caagcggccg ctgatcgaat
     9361 ccgcaaagaa tcccggcaac cgccggcagc cggtgcgccg tcgattagga agccgcccaa
     9421 gggcgacgag caaccagatt ttttcgttcc gatgctctat gacgtgggca cccgcgatag
     9481 tcgcagcatc atggacgtgg ccgttttccg tctgtcgaag cgtgaccgac gagctggcga
     9541 ggtgatccgc tacgagcttc cagacgggca cgtagaggtt tccgcagggc cggccggcat
     9601 ggccagtgtg tgggattacg acctggtact gatggcggtt tcccatctaa ccgaatccat
     9661 gaaccgatac cgggaaggga agggagacaa gcccggccgc gtgttccgtc cacacgttgc
     9721 ggacgtactc aagttctgcc ggcgagccga tggcggaaag cagaaagacg acctggtaga
     9781 aacctgcatt cggttaaaca ccacgcacgt tgccatgcag cgtacgaaga aggccaagaa
     9841 cggccgcctg gtgacggtat ccgagggtga agccttgatt agccgctaca agatcgtaaa
     9901 gagcgaaacc gggcggccgg agtacatcga gatcgagcta gctgattgga tgtaccgcga
     9961 gatcacagaa ggcaagaacc cggacgtgct gacggttcac cccgattact ttttgatcga
    10021 tcccggcatc ggccgttttc tctaccgcct ggcacgccgc gccgcaggca aggcagaagc
    10081 cagatggttg ttcaagacga tctacgaacg cagtggcagc gccggagagt tcaagaagtt
    10141 ctgtttcacc gtgcgcaagc tgatcgggtc aaatgacctg ccggagtacg atttgaagga
    10201 ggaggcgggg caggctggcc cgatcctagt catgcgctac cgcaacctga tcgagggcga
    10261 agcatccgcc ggttcctaat gtacggagca gatgctaggg caaattgccc tagcagggga
    10321 aaaaggtcga aaaggcctct ttcctgtgga tagcacgtac attgggaacc caaagccgta
    10381 cattgggaac cggaacccgt acattgggaa cccaaagccg tacattggga accggtcaca
    10441 catgtaagtg actgatataa aagagaaaaa aggcgatttt tccgcctaaa actctttaaa
    10501 acttattaaa actcttaaaa cccgcctggc ctgtgcataa ctgtctggcc agcgcacagc
    10561 cgaagagctg caaaaagcgc ctacccttcg gtcgctgcgc tccctacgcc ccgccgcttc
    10621 gcgtcggcct atcgcggccg ctggccgctc aaaaatggct ggcctacggc caggcaatct
    10681 accagggcgc ggacaagccg cgccgtcgcc actcgaccgc cggcgcccac atcaaggcac
    10741 cctgcctcgc gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaaa
    10801 cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag
    10861 cgggtgttgg cgggtgtcgg ggcgcagcca tgacccagtc acgtagcgat agcggagtgt
    10921 atactggctt aactatgcgg catcagagca gattgtactg agagtgcacc atatgcggtg
    10981 tgaaataccg cacagatgcg taaggagaaa ataccgcatc aggcgctctt ccgcttcctc
    11041 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa
    11101 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa
    11161 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
    11221 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
    11281 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
    11341 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
    11401 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
    11461 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
    11521 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
    11581 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
    11641 cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
    11701 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
    11761 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
    11821 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgcattctag
    11881 gtactaaaac aattcatcca gtaaaatata atattttatt ttctcccaat caggcttgat
    11941 ccccagtaag tcaaaaaata gctcgacata ctgttcttcc ccgatatcct ccctgatcga
    12001 ccggacgcag aaggcaatgt cataccactt gtccgccctg ccgcttctcc caagatcaat
    12061 aaagccactt actttgccat ctttcacaaa gatgttgctg tctcccaggt cgccgtggga
    12121 aaagacaagt tcctcttcgg gcttttccgt ctttaaaaaa tcatacagct cgcgcggatc
    12181 tttaaatgga gtgtcttctt cccagttttc gcaatccaca tcggccagat cgttattcag
    12241 taagtaatcc aattcggcta agcggctgtc taagctattc gtatagggac aatccgatat
    12301 gtcgatggag tgaaagagcc tgatgcactc cgcatacagc tcgataatct tttcagggct
    12361 ttgttcatct tcatactctt ccgagcaaag gacgccatcg gcctcactca tgagcagatt
    12421 gctccagcca tcatgccgtt caaagtgcag gacctttgga acaggcagct ttccttccag
    12481 ccatagcatc atgtcctttt cccgttccac atcataggtg gtccctttat accggctgtc
    12541 cgtcattttt aaatataggt tttcattttc tcccaccagc ttatatacct tagcaggaga
    12601 cattccttcc gtatctttta cgcagcggta tttttcgatc agttttttca attccggtga
    12661 tattctcatt ttagccattt attatttcct tcctcttttc tacagtattt aaagataccc
    12721 caagaagcta attataacaa gacgaactcc aattcactgt tccttgcatt ctaaaacctt
    12781 aaataccaga aaacagcttt ttcaaagttg ttttcaaagt tggcgtataa catagtatcg
    12841 acggagccga ttttgaaacc gcggtgatca caggcagcaa cgctctgtca tcgttacaat
    12901 caacatgcta ccctccgcga gatcatccgt gtttcaaacc cggcagctta gttgccgttc
    12961 ttccgaatag catcggtaac atgagcaaag tctgccgcct tacaacggct ctcccgctga
    13021 cgccgtcccg gactgatggg ctgcctgtat cgagtggtga ttttgtgccg agctgccggt
    13081 cggggagctg ttggctggct ggtggcagga tatattgtgg tgtaaacaaa ttgacgctta
    13141 gacaacttaa taacacattg cggacgtttt taatgtactg aattaacgcc gaattaattc
    13201 gggggatctg gattttagta ctggattttg gttttaggaa ttagaaattt tattgataga
    13261 agtattttac aaatacaaat acatactaag ggtttcttat atgctcaaca catgagcgaa
    13321 accctatagg aaccctaatt cccttatctg ggaactactc acacattatt atggagaaaa
    13381 tagagcttgt cgatcgttaa ttaatcacct ccaagatccc ggacgagtgc tggggcgtcg
    13441 gtttccacta tcggcgagta cttctacaca gccatcggtc cagacggccg cgcttctgcg
    13501 ggcgatttgt gtacgcccga cagtcccggc tccggatcgg acgattgcgt cgcatcgacc
    13561 ctgcgcccaa gctgcatcat cgaaattgcc gtcaaccaag ctctgataga gttggtcaag
    13621 accaatgcgg agcatatacg cccggagccg cggcgatcct gcaagctccg gatgcctccg
    13681 ctcgaagtag cgcgtctgct gctccataca agccaaccac ggcctccaga agaagatgtt
    13741 ggcgacctcg tattgggaat ccccgaacat cgcctcgctc cagtcaatga ccgctgttat
    13801 gcggccattg tccgtcagga cattgttgga gccgaaatcc gcgtgcacga ggtgccggac
    13861 ttcggggcag tcctcggccc aaagcatcag ctcatcgaga gcctgcgcga cggacgcact
    13921 gacggtgtcg tccatcacag tttgccagtg atacacatgg ggatcagcaa tcgcgcatat
    13981 gaaatcacgc catgtagtgt attgaccgat tccttgcggt ccgaatgggc cgaacccgct
    14041 cgtctggcta agatcggccg cagcgatcgc atccatggcc tccgcgaccg gctgcagaac
    14101 agcgggcagt tcggtttcag gcaggtcttg caacgtgaca ccctgtgcac ggcgggagat
    14161 gcaataggtc aggctctcgc tgaattcccc aatgtcaagc acttccggaa tcgggagcgc
    14221 ggccgatgca aagtgccgat aaacataacg atctttgtag aaaccatcgg cgcagctatt
    14281 tacccgcagg acatatccac gccctcctac atcgaagctg aaagcacgag attcttcgcc
    14341 ctccgagagc tgcatcaggt cggacacgct gtcgaacttt tcgatcagaa acttctcgac
    14401 agacgtcgcg gtgagttcag gctttttcat cctaggagag atagatttgt agagagagac
    14461 tggtgatttc agcgtgtcct ctccaaatga aatgaacttc cttatataga ggaaggtctt
    14521 gcgaaggata gtgggattgt gcgtcatccc ttacgtcagt ggagatatca catcaatcca
    14581 cttgctttga agacgtggtt ggaacgtctt ctttttccac gatgctcctc gtgggtgggg
    14641 gtccatcttt gggaccactg tcggcagagg catcttgaac gatagccttt cctttatcgc
    14701 aatgatggca tttgtaggtg ccaccttcct tttctactgt ccttttgatg aagtgacaga
    14761 tagctgggca atggaatccg aggaggtttc ccgatattac cctttgttga aaagtctcaa
    14821 tagccctttg gtcttctgag actgtatctt tgatattctt ggagtagacg agagtgtcgt
    14881 gctccaccat gttatcacat caatccactt gctttgaaga cgtggttgga acgtcttctt
    14941 tttccacgat gctcctcgtg ggtgggggtc catctttggg accactgtcg gcagaggcat
    15001 cttgaacgat agcctttcct ttatcgcaat gatggcattt gtaggtgcca ccttcctttt
    15061 ctactgtcct tttgatgaag tgacagatag ctgggcaatg gaatccgagg aggtttcccg
    15121 atattaccct ttgttgaaaa gtctcaatag ccctttggtc ttctgagact gtatctttga
    15181 tattcttgga gtagacgaga gtgtcgtgct ccaccatg
//
