LOCUS       sequence-266265-       15119 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  iSTU-CRISPR/Cas9 with StIV2 intron, NU structure backbone.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15119)
  AUTHORS   Zhong Z, Liu S, Liu X, Liu B, Tang X, Ren Q, Zhou J, Zheng X, Qi Y, 
            Zhang Y
  TITLE     Intron-Based Single Transcript Unit CRISPR Systems for Plant Genome 
            Editing.
  JOURNAL   Rice (N Y). 2020 Feb 3;13(1):8. doi: 10.1186/s12284-020-0369-8.
  PUBMED    32016614
REFERENCE   2  (bases 1 to 15119)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Rice (N 
            Y)."; date: "2020-02-3"; volume: "13(1):8. doi"; pages: " 
            10.1186/s12284-020-0369-8"
COMMENT     Sequence Label: pGEL077
FEATURES             Location/Qualifiers
     source          1..15119
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        8..2004
                     /label=Ubi promoter
                     /note="maize polyubiquitin gene promoter"
     misc_RNA        3504..3579
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     CDS             4029..6413
                     /codon_start=1
                     /product="C-terminal portion of Streptococcus pyogenes Cas9
                     (Zetsche et al., 2015)"
                     /label=Cas9(C)
                     /translation="CFDSVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILED
                     IVLTLTLFEDREMIEERLKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGK
                     TILDFLKSDGFANRNFMQLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKG
                     ILQTVKVVDELVKVMGRHKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQI
                     LKEHPVENTQLQNEKLYLYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDN
                     KVLTRSDKNRGKSDNVPSEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDK
                     AGFIKRQLVETRQITKHVAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQF
                     YKVREINNYHHAHDAYLNAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGK
                     ATAKYFFYSNIMNFFKTEITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMP
                     QVNIVKKTEVQTGGFSKESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKV
                     EKGKSKKLKSVKELLGITIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELEN
                     GRKRMLASAGELQKGNELALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLD
                     EIIEQISEFSKRVILADANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKY
                     FDTTIDRKRYTSTKEVLDATLIHQSITGLYETRIDLSQLGGD"
     CDS             6426..6446
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     terminator      6457..6705
                     /label=HSP terminator
                     /note="efficient transcription terminator from the 
                     Arabidopsis thaliana heat shock protein 18.2 gene (Nagaya 
                     et al., 2010)"
     misc_feature    6726..6750
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             8050..8679
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             9113..10180
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQA
                     AADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKR
                     DRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGR
                     VFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEAL
                     ISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLA
                     RRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILV
                     MRYRNLIEGEASAGS"
     rep_origin      10246..10440
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(10676..10698)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    10784..10924
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     10839..10858
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(10939..10956)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(11110..11698)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11190..11209)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(11785..12579)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    13004..13028
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    13106..13280
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     promoter        complement(14370..15047)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
ORIGIN
        1 ggcgcgcctg cagtgcagcg tgacccggtc gtgcccctct cttgagataa tgagcattgc
       61 atgtctaagt tataaaaaat taccacatat tttttttgtc acacttgttt gaagtgcagt
      121 ttatctatct ttatacatat atttaaactt tactctacga ataatataat ctatagtact
      181 acaataatat cagtgtttta gagaatcata taaatgaaca gttagacatg gtctaaagga
      241 caattgagta ttttgacaac aggactctac agttttatct ttttagtgtg catgtgttct
      301 cctttttttt tgcaaatagc ttcacctata taatacttca tccattttat tagtacatcc
      361 atttagggtt tagggttaat ggtttttata gactaatttt tttagtacat ctattttatt
      421 ctattttagc ctctaaatta agaaaactaa aactctattt tagttttttt atttaataat
      481 ttagatataa aatagaataa aataaagtga ctaaaaatta aacaaatacc ctttaagaaa
      541 ttaaaaaaac taaggaaaca tttttcttgt ttcgagtaga taatgccagc ctgttaaacg
      601 ccgtcgacga gtctaacgga caccaaccag cgaaccagca gcgtcgcgtc gggccaagcg
      661 aagcagacgg cacggcatct ctgtcgctgc ctctggaccc ctctcgagag ttccgctcca
      721 ccgttggact tgctccgctg tcggcatcca gaaattgcgt ggcggagcgg cagacgtgag
      781 ccggcacggc aggcggcctc ctcctcctct cacggcaccg gcagctacgg gggattcctt
      841 tcccaccgct ccttcgcttt cccttcctcg cccgccgtaa taaatagaca ccccctccac
      901 accctctttc cccaacctcg tgttgttcgg agcgcacaca cacacaacca gaactccccc
      961 aaatccaccc gtcggcacct ccgcttcaag gtacgccgct cgtcctcccc ccccccccct
     1021 ctctaccttc tcaagatcgg cgttccggtc catggttagg gcccggtagt tctacttctg
     1081 ttcatgtttg tgttagatcc gtgtttgtgt tagatccgtg ctactagcgt tcgtacacgg
     1141 atgcgacctg tacgtcagac acgttctgat tgctaacttg ccagtgtttc tctttgggga
     1201 atcctgggat ggctctagcc gttccgcaga cgggatcgat ttcatgattt tttttgtttc
     1261 gttgcatagg gtttggtttg cccttttcct ttatttcaat atatgccgtg cacttgtttg
     1321 tcgggtcatc ttttcatgct tttttttgtc ttggttgtga tgatgtggtc tggttgggcg
     1381 gtcgttcaag atcggagtag aattaattct gtttcaaact acctggtgga tttattaatt
     1441 ttggatctgt atgtgtgtgc catacatatt catagttacg aattgaagat gatggatgga
     1501 aatatcgatc taggataggt atacatgttg atgcgggttt tactgatgca tatacagaga
     1561 tgctttttgt tcgcttggtt gtgatgatgt ggtgtggttg ggcggtcgtt cattcgttca
     1621 agatcggagt agaatactgt ttcaaactac ctggtgtatt tattaatttt ggaactgtat
     1681 gtgtgtgtca tacatcttca tagttacgag tttaagatgg atggaaatat cgatctagga
     1741 taggtataca tgttgatgtg ggttttactg atgcatatac atgatggcat atgcagcatc
     1801 tattcatatg ctctaacctt gagtacctat ctattataat aaacaagtat gttttataat
     1861 tattttgatc ttgatatact tggatgatgg catatgcagc agctatatgt ggattttttt
     1921 agccctgcct tcatacgcta tttatttgct tggtactgtt tcttttgtcg atgctcaccc
     1981 tgttgtttgg tgttacttct gcagcctgca ggtagatcgc tcgtcgacat ggataagaag
     2041 tactctatcg gactcgatat cggaactaac tctgtgggat gggctgtgat caccgatgag
     2101 tacaaggtgc catctaagaa gttcaaggtt ctcggaaaca ccgataggca ctctatcaag
     2161 aaaaacctta tcggtgctct cctcttcgat tctggtgaaa ctgctgaggc taccagactc
     2221 aagagaaccg ctagaagaag gtacaccaga agaaagaaca ggatctgcta cctccaagag
     2281 atcttctcta acgagatggc taaagtggat gattcattct tccacaggct cgaagagtca
     2341 ttcctcgtgg aagaagataa gaagcacgag aggcacccta tcttcggaaa catcgttgat
     2401 gaggtggcat accacgagaa gtaccctact atctaccacc tcagaaagaa gctcgttgat
     2461 tctactgata aggctgatct caggctcatc tacctcgctc tcgctcacat gatcaagttc
     2521 agaggacact tcctcatcga gggtgatctc aaccctgata actctgatgt ggataagttg
     2581 ttcatccagc tcgtgcagac ctacaaccag cttttcgaag agaaccctat caacgcttca
     2641 ggtgtggatg ctaaggctat cctctctgct aggctctcta agtcaagaag gcttgagaac
     2701 ctcattgctc agctccctgg tgagaagaag aacggacttt tcggaaactt gatcgctctc
     2761 tctctcggac tcacccctaa cttcaagtct aacttcgatc tcgctgagga tgcaaagctc
     2821 cagctctcaa aggataccta cgatgatgat ctcgataacc tcctcgctca gatcggagat
     2881 cagtacgctg atttgttcct cgctgctaag aacctctctg atgctatcct cctcagtgat
     2941 atcctcagag tgaacaccga gatcaccaag gctccactct cagcttctat gatcaagaga
     3001 tacgatgagc accaccagga tctcacactt ctcaaggctc ttgttagaca gcagctccca
     3061 gagaagtaca aagagatttt cttcgatcag tctaagaacg gatacgctgg ttacatcgat
     3121 ggtggtgcat ctcaagaaga gttctacaag ttcatcaagc ctatcctcga gaagatggat
     3181 ggaaccgagg aactcctcgt gaagctcaat agagaggatc ttctcagaaa gcagaggacc
     3241 ttcgataacg gatctatccc tcatcagatc cacctcggag agttgcacgc tatccttaga
     3301 aggcaagagg atttctaccc attcctcaag gataacaggg aaaagattga gaagattctc
     3361 accttcagaa tcccttacta cgtgggacct ctcgctagag gaaactcaag attcgcttgg
     3421 atgaccagaa agtctgagga aaccatcacc ccttggaact tcgaagaggt aagtttctgc
     3481 ttctaccgga gaccggggtc tcagttttag agctagaaat agcaagttaa aataaggcta
     3541 gtccgttatc aacttgaaaa agtggcaccg agtcggtgct ttgatatata tataataatt
     3601 atcattaatt agtagtaata taatatttca aatatttttt tcaaaataaa agaatgtagt
     3661 atatagcaat tgcttttctg tagtttataa gtgtgtatat tttaatttat aacttttcta
     3721 atatatgacc aaaatttgtt gatgtgcagg tggtggataa gggtgctagt gctcagtctt
     3781 tcatcgagag gatgaccaac ttcgataaga accttccaaa cgagaaggtg ctccctaagc
     3841 actctttgct ctacgagtac ttcaccgtgt acaacgagtt gaccaaggtt aagtacgtga
     3901 ccgagggaat gaggaagcct gcttttttgt caggtgagca aaagaaggct atcgttgatc
     3961 tcttgttcaa gaccaacaga aaggtgaccg tgaagcagct caaagaggat tacttcaaga
     4021 aaatcgagtg cttcgattca gttgagattt ctggtgttga ggataggttc aacgcatctc
     4081 tcggaaccta ccacgatctc ctcaagatca ttaaggataa ggatttcttg gataacgagg
     4141 aaaacgagga tatcttggag gatatcgttc ttaccctcac cctctttgaa gatagagaga
     4201 tgattgaaga aaggctcaag acctacgctc atctcttcga tgataaggtg atgaagcagt
     4261 tgaagagaag aagatacact ggttggggaa ggctctcaag aaagctcatt aacggaatca
     4321 gggataagca gtctggaaag acaatccttg atttcctcaa gtctgatgga ttcgctaaca
     4381 gaaacttcat gcagctcatc cacgatgatt ctctcacctt taaagaggat atccagaagg
     4441 ctcaggtttc aggacagggt gatagtctcc atgagcatat cgctaacctc gctggatctc
     4501 ctgcaatcaa gaagggaatc ctccagactg tgaaggttgt ggatgagttg gtgaaggtga
     4561 tgggaaggca taagcctgag aacatcgtga tcgaaatggc tagagagaac cagaccactc
     4621 agaagggaca gaagaactct agggaaagga tgaagaggat cgaggaaggt atcaaagagc
     4681 ttggatctca gatcctcaaa gagcaccctg ttgagaacac tcagctccag aatgagaagc
     4741 tctacctcta ctacctccag aacggaaggg atatgtatgt ggatcaagag ttggatatca
     4801 acaggctctc tgattacgat gttgatcata tcgtgccaca gtcattcttg aaggatgatt
     4861 ctatcgataa caaggtgctc accaggtctg ataagaacag gggtaagagt gataacgtgc
     4921 caagtgaaga ggttgtgaag aaaatgaaga actattggag gcagctcctc aacgctaagc
     4981 tcatcactca gagaaagttc gataacttga ctaaggctga gaggggagga ctctctgaat
     5041 tggataaggc aggattcatc aagaggcagc ttgtggaaac caggcagatc actaagcacg
     5101 ttgcacagat cctcgattct aggatgaaca ccaagtacga tgagaacgat aagttgatca
     5161 gggaagtgaa ggttatcacc ctcaagtcaa agctcgtgtc tgatttcaga aaggatttcc
     5221 aattctacaa ggtgagggaa atcaacaact accaccacgc tcacgatgct taccttaacg
     5281 ctgttgttgg aaccgctctc atcaagaagt atcctaagct cgagtcagag ttcgtgtacg
     5341 gtgattacaa ggtgtacgat gtgaggaaga tgatcgctaa gtctgagcaa gagatcggaa
     5401 aggctaccgc taagtatttc ttctactcta acatcatgaa tttcttcaag accgagatta
     5461 ccctcgctaa cggtgagatc agaaagaggc cactcatcga gacaaacggt gaaacaggtg
     5521 agatcgtgtg ggataaggga agggatttcg ctaccgttag aaaggtgctc tctatgccac
     5581 aggtgaacat cgttaagaaa accgaggtgc agaccggtgg attctctaaa gagtctatcc
     5641 tccctaagag gaactctgat aagctcattg ctaggaagaa ggattgggac cctaagaaat
     5701 acggtggttt cgattctcct accgtggctt actctgttct cgttgtggct aaggttgaga
     5761 agggaaagag taagaagctc aagtctgtta aggaacttct cggaatcact atcatggaaa
     5821 ggtcatcttt cgagaagaac ccaatcgatt tcctcgaggc taagggatac aaagaggtta
     5881 agaaggatct catcatcaag ctcccaaagt actcactctt cgaactcgag aacggtagaa
     5941 agaggatgct cgcttctgct ggtgagcttc aaaagggaaa cgagcttgct ctcccatcta
     6001 agtacgttaa ctttctttac ctcgcttctc actacgagaa gttgaaggga tctccagaag
     6061 ataacgagca gaagcaactt ttcgttgagc agcacaagca ctacttggat gagatcatcg
     6121 agcagatctc tgagttctct aaaagggtga tcctcgctga tgcaaacctc gataaggtgt
     6181 tgtctgctta caacaagcac agagataagc ctatcaggga acaggcagag aacatcatcc
     6241 atctcttcac ccttaccaac ctcggtgctc ctgctgcttt caagtacttc gatacaacca
     6301 tcgataggaa gagatacacc tctaccaaag aagtgctcga tgctaccctc atccatcagt
     6361 ctatcactgg actctacgag actaggatcg atctctcaca gctcggtggt gattcaaggg
     6421 ctgatcctaa gaagaagagg aaggtttgac tcgagatatg aagatgaaga tgaaatattt
     6481 ggtgtgtcaa ataaaaagct tgtgtgctta agtttgtgtt tttttcttgg cttgttgtgt
     6541 tatgaatttg tggctttttc taatattaaa tgaatgtaag atcacattat aatgaataaa
     6601 caaatgtttc tataatccat tgtgaatgtt ttgttggatc tcttctgcag catataacta
     6661 ctgtatgtgc tatggtatgg actatggaat atgattaaag ataaggagct caaactatca
     6721 gtgtttgaca ggatatattg gcgggtaaac ctaagagaaa agagcgttta ttagaataac
     6781 ggatatttaa aagggcgtga aaaggtttat ccgttcgtcc atttgtatgt gcatgccaac
     6841 cacagggttc ccctcgggat caaagtactt tgatccaacc cctccgctgc tatagtgcag
     6901 tcggcttctg acgttcagtg cagccgtctt ctgaaaacga catgtcgcac aagtcctaag
     6961 ttacgcgaca ggctgccgcc ctgccctttt cctggcgttt tcttgtcgcg tgttttagtc
     7021 gcataaagta gaatacttgc gactagaacc ggagacatta cgccatgaac aagagcgccg
     7081 ccgctggcct gctgggctat gcccgcgtca gcaccgacga ccaggacttg accaaccaac
     7141 gggccgaact gcacgcggcc ggctgcacca agctgttttc cgagaagatc accggcacca
     7201 ggcgcgaccg cccggagctg gccaggatgc ttgaccacct acgccctggc gacgttgtga
     7261 cagtgaccag gctagaccgc ctggcccgca gcacccgcga cctactggac attgccgagc
     7321 gcatccagga ggccggcgcg ggcctgcgta gcctggcaga gccgtgggcc gacaccacca
     7381 cgccggccgg ccgcatggtg ttgaccgtgt tcgccggcat tgccgagttc gagcgttccc
     7441 taatcatcga ccgcacccgg agcgggcgcg aggccgccaa ggcccgaggc gtgaagtttg
     7501 gcccccgccc taccctcacc ccggcacaga tcgcgcacgc ccgcgagctg atcgaccagg
     7561 aaggccgcac cgtgaaagag gcggctgcac tgcttggcgt gcatcgctcg accctgtacc
     7621 gcgcacttga gcgcagcgag gaagtgacgc ccaccgaggc caggcggcgc ggtgccttcc
     7681 gtgaggacgc attgaccgag gccgacgccc tggcggccgc cgagaatgaa cgccaagagg
     7741 aacaagcatg aaaccgcacc aggacggcca ggacgaaccg tttttcatta ccgaagagat
     7801 cgaggcggag atgatcgcgg ccgggtacgt gttcgagccg cccgcgcacg gctcaaccgt
     7861 gcggctgcat gaaatcctgg ccggtttgtc tgatgccaag ctggcggcct ggccggccag
     7921 cttggccgct gaagaaaccg agcgccgccg tctaaaaagg tgatgtgtat ttgagtaaaa
     7981 cagcttgcgt catgcggtcg ctgcgtatat gatgcgatga gtaaataaac aaatacgcaa
     8041 ggggaacgca tgaaggttat cgctgtactt aaccagaaag gcgggtcagg caagacgacc
     8101 atcgcaaccc atctagcccg cgccctgcaa ctcgccgggg ccgatgttct gttagtcgat
     8161 tccgatcccc agggcagtgc ccgcgattgg gcggccgtgc gggaagatca accgctaacc
     8221 gttgtcggca tcgaccgccc gacgattgac cgcgacgtga aggccatcgg ccggcgcgac
     8281 ttcgtagtga tcgacggagc gccccaggcg gcggacttgg ctgtgtccgc gatcaaggca
     8341 gccgacttcg tgctgattcc ggtgcagcca agcccttacg acatatgggc caccgccgac
     8401 ctggtggagc tggttaagca gcgcattgag gtcacggatg gaaggctaca agcggccttt
     8461 gtcgtgtcgc gggcgatcaa aggcacgcgc atcggcggtg aggttgccga ggcgctggcc
     8521 gggtacgagc tgcccattct tgagtcccgt atcacgcagc gcgtgagcta cccaggcact
     8581 gccgccgccg gcacaaccgt tcttgaatca gaacccgagg gcgacgctgc ccgcgaggtc
     8641 caggcgctgg ccgctgaaat taaatcaaaa ctcatttgag ttaatgaggt aaagagaaaa
     8701 tgagcaaaag cacaaacacg ctaagtgccg gccgtccgag cgcacgcagc agcaaggctg
     8761 caacgttggc cagcctggca gacacgccag ccatgaagcg ggtcaacttt cagttgccgg
     8821 cggaggatca caccaagctg aagatgtacg cggtacgcca aggcaagacc attaccgagc
     8881 tgctatctga atacatcgcg cagctaccag agtaaatgag caaatgaata aatgagtaga
     8941 tgaattttag cggctaaagg aggcggcatg gaaaatcaag aacaaccagg caccgacgcc
     9001 gtggaatgcc ccatgtgtgg aggaacgggc ggttggccag gcgtaagcgg ctgggttgtc
     9061 tgccggccct gcaatggcac tggaaccccc aagcccgagg aatcggcgtg acggtcgcaa
     9121 accatccggc ccggtacaaa tcggcgcggc gctgggtgat gacctggtgg agaagttgaa
     9181 ggccgcgcag gccgcccagc ggcaacgcat cgaggcagaa gcacgccccg gtgaatcgtg
     9241 gcaagcggcc gctgatcgaa tccgcaaaga atcccggcaa ccgccggcag ccggtgcgcc
     9301 gtcgattagg aagccgccca agggcgacga gcaaccagat tttttcgttc cgatgctcta
     9361 tgacgtgggc acccgcgata gtcgcagcat catggacgtg gccgttttcc gtctgtcgaa
     9421 gcgtgaccga cgagctggcg aggtgatccg ctacgagctt ccagacgggc acgtagaggt
     9481 ttccgcaggg ccggccggca tggccagtgt gtgggattac gacctggtac tgatggcggt
     9541 ttcccatcta accgaatcca tgaaccgata ccgggaaggg aagggagaca agcccggccg
     9601 cgtgttccgt ccacacgttg cggacgtact caagttctgc cggcgagccg atggcggaaa
     9661 gcagaaagac gacctggtag aaacctgcat tcggttaaac accacgcacg ttgccatgca
     9721 gcgtacgaag aaggccaaga acggccgcct ggtgacggta tccgagggtg aagccttgat
     9781 tagccgctac aagatcgtaa agagcgaaac cgggcggccg gagtacatcg agatcgagct
     9841 agctgattgg atgtaccgcg agatcacaga aggcaagaac ccggacgtgc tgacggttca
     9901 ccccgattac tttttgatcg atcccggcat cggccgtttt ctctaccgcc tggcacgccg
     9961 cgccgcaggc aaggcagaag ccagatggtt gttcaagacg atctacgaac gcagtggcag
    10021 cgccggagag ttcaagaagt tctgtttcac cgtgcgcaag ctgatcgggt caaatgacct
    10081 gccggagtac gatttgaagg aggaggcggg gcaggctggc ccgatcctag tcatgcgcta
    10141 ccgcaacctg atcgagggcg aagcatccgc cggttcctaa tgtacggagc agatgctagg
    10201 gcaaattgcc ctagcagggg aaaaaggtcg aaaaggcctc tttcctgtgg atagcacgta
    10261 cattgggaac ccaaagccgt acattgggaa ccggaacccg tacattggga acccaaagcc
    10321 gtacattggg aaccggtcac acatgtaagt gactgatata aaagagaaaa aaggcgattt
    10381 ttccgcctaa aactctttaa aacttattaa aactcttaaa acccgcctgg cctgtgcata
    10441 actgtctggc cagcgcacag ccgaagagct gcaaaaagcg cctacccttc ggtcgctgcg
    10501 ctccctacgc cccgccgctt cgcgtcggcc tatcgcggcc gctggccgct caaaaatggc
    10561 tggcctacgg ccaggcaatc taccagggcg cggacaagcc gcgccgtcgc cactcgaccg
    10621 ccggcgccca catcaaggca ccctgcctcg cgcgtttcgg tgatgacggt gaaaacctct
    10681 gacacatgca gctcccggaa acggtcacag cttgtctgta agcggatgcc gggagcagac
    10741 aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg gggcgcagcc atgacccagt
    10801 cacgtagcga tagcggagtg tatactggct taactatgcg gcatcagagc agattgtact
    10861 gagagtgcac catatgcggt gtgaaatacc gcacagatgc gtaaggagaa aataccgcat
    10921 caggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
    10981 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
    11041 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
    11101 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
    11161 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
    11221 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
    11281 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
    11341 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
    11401 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
    11461 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
    11521 gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa
    11581 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
    11641 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
    11701 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
    11761 gattttggtc atgcattcta ggtactaaaa caattcatcc agtaaaatat aatattttat
    11821 tttctcccaa tcaggcttga tccccagtaa gtcaaaaaat agctcgacat actgttcttc
    11881 cccgatatcc tccctgatcg accggacgca gaaggcaatg tcataccact tgtccgccct
    11941 gccgcttctc ccaagatcaa taaagccact tactttgcca tctttcacaa agatgttgct
    12001 gtctcccagg tcgccgtggg aaaagacaag ttcctcttcg ggcttttccg tctttaaaaa
    12061 atcatacagc tcgcgcggat ctttaaatgg agtgtcttct tcccagtttt cgcaatccac
    12121 atcggccaga tcgttattca gtaagtaatc caattcggct aagcggctgt ctaagctatt
    12181 cgtataggga caatccgata tgtcgatgga gtgaaagagc ctgatgcact ccgcatacag
    12241 ctcgataatc ttttcagggc tttgttcatc ttcatactct tccgagcaaa ggacgccatc
    12301 ggcctcactc atgagcagat tgctccagcc atcatgccgt tcaaagtgca ggacctttgg
    12361 aacaggcagc tttccttcca gccatagcat catgtccttt tcccgttcca catcataggt
    12421 ggtcccttta taccggctgt ccgtcatttt taaatatagg ttttcatttt ctcccaccag
    12481 cttatatacc ttagcaggag acattccttc cgtatctttt acgcagcggt atttttcgat
    12541 cagttttttc aattccggtg atattctcat tttagccatt tattatttcc ttcctctttt
    12601 ctacagtatt taaagatacc ccaagaagct aattataaca agacgaactc caattcactg
    12661 ttccttgcat tctaaaacct taaataccag aaaacagctt tttcaaagtt gttttcaaag
    12721 ttggcgtata acatagtatc gacggagccg attttgaaac cgcggtgatc acaggcagca
    12781 acgctctgtc atcgttacaa tcaacatgct accctccgcg agatcatccg tgtttcaaac
    12841 ccggcagctt agttgccgtt cttccgaata gcatcggtaa catgagcaaa gtctgccgcc
    12901 ttacaacggc tctcccgctg acgccgtccc ggactgatgg gctgcctgta tcgagtggtg
    12961 attttgtgcc gagctgccgg tcggggagct gttggctggc tggtggcagg atatattgtg
    13021 gtgtaaacaa attgacgctt agacaactta ataacacatt gcggacgttt ttaatgtact
    13081 gaattaacgc cgaattaatt cgggggatct ggattttagt actggatttt ggttttagga
    13141 attagaaatt ttattgatag aagtatttta caaatacaaa tacatactaa gggtttctta
    13201 tatgctcaac acatgagcga aaccctatag gaaccctaat tcccttatct gggaactact
    13261 cacacattat tatggagaaa atagagcttg tcgatcgtta attaatcacc tccaagatcc
    13321 cggacgagtg ctggggcgtc ggtttccact atcggcgagt acttctacac agccatcggt
    13381 ccagacggcc gcgcttctgc gggcgatttg tgtacgcccg acagtcccgg ctccggatcg
    13441 gacgattgcg tcgcatcgac cctgcgccca agctgcatca tcgaaattgc cgtcaaccaa
    13501 gctctgatag agttggtcaa gaccaatgcg gagcatatac gcccggagcc gcggcgatcc
    13561 tgcaagctcc ggatgcctcc gctcgaagta gcgcgtctgc tgctccatac aagccaacca
    13621 cggcctccag aagaagatgt tggcgacctc gtattgggaa tccccgaaca tcgcctcgct
    13681 ccagtcaatg accgctgtta tgcggccatt gtccgtcagg acattgttgg agccgaaatc
    13741 cgcgtgcacg aggtgccgga cttcggggca gtcctcggcc caaagcatca gctcatcgag
    13801 agcctgcgcg acggacgcac tgacggtgtc gtccatcaca gtttgccagt gatacacatg
    13861 gggatcagca atcgcgcata tgaaatcacg ccatgtagtg tattgaccga ttccttgcgg
    13921 tccgaatggg ccgaacccgc tcgtctggct aagatcggcc gcagcgatcg catccatggc
    13981 ctccgcgacc ggctgcagaa cagcgggcag ttcggtttca ggcaggtctt gcaacgtgac
    14041 accctgtgca cggcgggaga tgcaataggt caggctctcg ctgaattccc caatgtcaag
    14101 cacttccgga atcgggagcg cggccgatgc aaagtgccga taaacataac gatctttgta
    14161 gaaaccatcg gcgcagctat ttacccgcag gacatatcca cgccctccta catcgaagct
    14221 gaaagcacga gattcttcgc cctccgagag ctgcatcagg tcggacacgc tgtcgaactt
    14281 ttcgatcaga aacttctcga cagacgtcgc ggtgagttca ggctttttca tcctaggaga
    14341 gatagatttg tagagagaga ctggtgattt cagcgtgtcc tctccaaatg aaatgaactt
    14401 ccttatatag aggaaggtct tgcgaaggat agtgggattg tgcgtcatcc cttacgtcag
    14461 tggagatatc acatcaatcc acttgctttg aagacgtggt tggaacgtct tctttttcca
    14521 cgatgctcct cgtgggtggg ggtccatctt tgggaccact gtcggcagag gcatcttgaa
    14581 cgatagcctt tcctttatcg caatgatggc atttgtaggt gccaccttcc ttttctactg
    14641 tccttttgat gaagtgacag atagctgggc aatggaatcc gaggaggttt cccgatatta
    14701 ccctttgttg aaaagtctca atagcccttt ggtcttctga gactgtatct ttgatattct
    14761 tggagtagac gagagtgtcg tgctccacca tgttatcaca tcaatccact tgctttgaag
    14821 acgtggttgg aacgtcttct ttttccacga tgctcctcgt gggtgggggt ccatctttgg
    14881 gaccactgtc ggcagaggca tcttgaacga tagcctttcc tttatcgcaa tgatggcatt
    14941 tgtaggtgcc accttccttt tctactgtcc ttttgatgaa gtgacagata gctgggcaat
    15001 ggaatccgag gaggtttccc gatattaccc tttgttgaaa agtctcaata gccctttggt
    15061 cttctgagac tgtatctttg atattcttgg agtagacgag agtgtcgtgc tccaccatg
//
