LOCUS       sequence-266266-       15291 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  iSTU-CRISPR/Cas9 with OsCDPK2intron, RZ structure backbone.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15291)
  AUTHORS   Zhong Z, Liu S, Liu X, Liu B, Tang X, Ren Q, Zhou J, Zheng X, Qi Y, 
            Zhang Y
  TITLE     Intron-Based Single Transcript Unit CRISPR Systems for Plant Genome 
            Editing.
  JOURNAL   Rice (N Y). 2020 Feb 3;13(1):8. doi: 10.1186/s12284-020-0369-8.
  PUBMED    32016614
REFERENCE   2  (bases 1 to 15291)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Rice (N 
            Y)."; date: "2020-02-3"; volume: "13(1):8. doi"; pages: " 
            10.1186/s12284-020-0369-8"
COMMENT     Sequence Label: pGEL082
FEATURES             Location/Qualifiers
     source          1..15291
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        8..2004
                     /label=Ubi promoter
                     /note="maize polyubiquitin gene promoter"
     misc_RNA        3521..3596
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     CDS             4201..6585
                     /codon_start=1
                     /product="C-terminal portion of Streptococcus pyogenes Cas9
                     (Zetsche et al., 2015)"
                     /label=Cas9(C)
                     /translation="CFDSVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILED
                     IVLTLTLFEDREMIEERLKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGK
                     TILDFLKSDGFANRNFMQLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKG
                     ILQTVKVVDELVKVMGRHKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQI
                     LKEHPVENTQLQNEKLYLYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDN
                     KVLTRSDKNRGKSDNVPSEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDK
                     AGFIKRQLVETRQITKHVAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQF
                     YKVREINNYHHAHDAYLNAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGK
                     ATAKYFFYSNIMNFFKTEITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMP
                     QVNIVKKTEVQTGGFSKESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKV
                     EKGKSKKLKSVKELLGITIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELEN
                     GRKRMLASAGELQKGNELALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLD
                     EIIEQISEFSKRVILADANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKY
                     FDTTIDRKRYTSTKEVLDATLIHQSITGLYETRIDLSQLGGD"
     CDS             6598..6618
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     terminator      6629..6877
                     /label=HSP terminator
                     /note="efficient transcription terminator from the 
                     Arabidopsis thaliana heat shock protein 18.2 gene (Nagaya 
                     et al., 2010)"
     misc_feature    6898..6922
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             8222..8851
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             9285..10352
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQA
                     AADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKR
                     DRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGR
                     VFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEAL
                     ISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLA
                     RRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILV
                     MRYRNLIEGEASAGS"
     rep_origin      10418..10612
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(10848..10870)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    10956..11096
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     11011..11030
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(11111..11128)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(11282..11870)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11362..11381)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(11957..12751)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    13176..13200
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    13278..13452
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     promoter        complement(14542..15219)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
ORIGIN
        1 ggcgcgcctg cagtgcagcg tgacccggtc gtgcccctct cttgagataa tgagcattgc
       61 atgtctaagt tataaaaaat taccacatat tttttttgtc acacttgttt gaagtgcagt
      121 ttatctatct ttatacatat atttaaactt tactctacga ataatataat ctatagtact
      181 acaataatat cagtgtttta gagaatcata taaatgaaca gttagacatg gtctaaagga
      241 caattgagta ttttgacaac aggactctac agttttatct ttttagtgtg catgtgttct
      301 cctttttttt tgcaaatagc ttcacctata taatacttca tccattttat tagtacatcc
      361 atttagggtt tagggttaat ggtttttata gactaatttt tttagtacat ctattttatt
      421 ctattttagc ctctaaatta agaaaactaa aactctattt tagttttttt atttaataat
      481 ttagatataa aatagaataa aataaagtga ctaaaaatta aacaaatacc ctttaagaaa
      541 ttaaaaaaac taaggaaaca tttttcttgt ttcgagtaga taatgccagc ctgttaaacg
      601 ccgtcgacga gtctaacgga caccaaccag cgaaccagca gcgtcgcgtc gggccaagcg
      661 aagcagacgg cacggcatct ctgtcgctgc ctctggaccc ctctcgagag ttccgctcca
      721 ccgttggact tgctccgctg tcggcatcca gaaattgcgt ggcggagcgg cagacgtgag
      781 ccggcacggc aggcggcctc ctcctcctct cacggcaccg gcagctacgg gggattcctt
      841 tcccaccgct ccttcgcttt cccttcctcg cccgccgtaa taaatagaca ccccctccac
      901 accctctttc cccaacctcg tgttgttcgg agcgcacaca cacacaacca gaactccccc
      961 aaatccaccc gtcggcacct ccgcttcaag gtacgccgct cgtcctcccc ccccccccct
     1021 ctctaccttc tcaagatcgg cgttccggtc catggttagg gcccggtagt tctacttctg
     1081 ttcatgtttg tgttagatcc gtgtttgtgt tagatccgtg ctactagcgt tcgtacacgg
     1141 atgcgacctg tacgtcagac acgttctgat tgctaacttg ccagtgtttc tctttgggga
     1201 atcctgggat ggctctagcc gttccgcaga cgggatcgat ttcatgattt tttttgtttc
     1261 gttgcatagg gtttggtttg cccttttcct ttatttcaat atatgccgtg cacttgtttg
     1321 tcgggtcatc ttttcatgct tttttttgtc ttggttgtga tgatgtggtc tggttgggcg
     1381 gtcgttcaag atcggagtag aattaattct gtttcaaact acctggtgga tttattaatt
     1441 ttggatctgt atgtgtgtgc catacatatt catagttacg aattgaagat gatggatgga
     1501 aatatcgatc taggataggt atacatgttg atgcgggttt tactgatgca tatacagaga
     1561 tgctttttgt tcgcttggtt gtgatgatgt ggtgtggttg ggcggtcgtt cattcgttca
     1621 agatcggagt agaatactgt ttcaaactac ctggtgtatt tattaatttt ggaactgtat
     1681 gtgtgtgtca tacatcttca tagttacgag tttaagatgg atggaaatat cgatctagga
     1741 taggtataca tgttgatgtg ggttttactg atgcatatac atgatggcat atgcagcatc
     1801 tattcatatg ctctaacctt gagtacctat ctattataat aaacaagtat gttttataat
     1861 tattttgatc ttgatatact tggatgatgg catatgcagc agctatatgt ggattttttt
     1921 agccctgcct tcatacgcta tttatttgct tggtactgtt tcttttgtcg atgctcaccc
     1981 tgttgtttgg tgttacttct gcagcctgca ggtagatcgc tcgtcgacat ggataagaag
     2041 tactctatcg gactcgatat cggaactaac tctgtgggat gggctgtgat caccgatgag
     2101 tacaaggtgc catctaagaa gttcaaggtt ctcggaaaca ccgataggca ctctatcaag
     2161 aaaaacctta tcggtgctct cctcttcgat tctggtgaaa ctgctgaggc taccagactc
     2221 aagagaaccg ctagaagaag gtacaccaga agaaagaaca ggatctgcta cctccaagag
     2281 atcttctcta acgagatggc taaagtggat gattcattct tccacaggct cgaagagtca
     2341 ttcctcgtgg aagaagataa gaagcacgag aggcacccta tcttcggaaa catcgttgat
     2401 gaggtggcat accacgagaa gtaccctact atctaccacc tcagaaagaa gctcgttgat
     2461 tctactgata aggctgatct caggctcatc tacctcgctc tcgctcacat gatcaagttc
     2521 agaggacact tcctcatcga gggtgatctc aaccctgata actctgatgt ggataagttg
     2581 ttcatccagc tcgtgcagac ctacaaccag cttttcgaag agaaccctat caacgcttca
     2641 ggtgtggatg ctaaggctat cctctctgct aggctctcta agtcaagaag gcttgagaac
     2701 ctcattgctc agctccctgg tgagaagaag aacggacttt tcggaaactt gatcgctctc
     2761 tctctcggac tcacccctaa cttcaagtct aacttcgatc tcgctgagga tgcaaagctc
     2821 cagctctcaa aggataccta cgatgatgat ctcgataacc tcctcgctca gatcggagat
     2881 cagtacgctg atttgttcct cgctgctaag aacctctctg atgctatcct cctcagtgat
     2941 atcctcagag tgaacaccga gatcaccaag gctccactct cagcttctat gatcaagaga
     3001 tacgatgagc accaccagga tctcacactt ctcaaggctc ttgttagaca gcagctccca
     3061 gagaagtaca aagagatttt cttcgatcag tctaagaacg gatacgctgg ttacatcgat
     3121 ggtggtgcat ctcaagaaga gttctacaag ttcatcaagc ctatcctcga gaagatggat
     3181 ggaaccgagg aactcctcgt gaagctcaat agagaggatc ttctcagaaa gcagaggacc
     3241 ttcgataacg gatctatccc tcatcagatc cacctcggag agttgcacgc tatccttaga
     3301 aggcaagagg atttctaccc attcctcaag gataacaggg aaaagattga gaagattctc
     3361 accttcagaa tcccttacta cgtgggacct ctcgctagag gaaactcaag attcgcttgg
     3421 atgaccagaa agtctgagga aaccatcacc ccttggaact tcgaagaggt caatatgctg
     3481 tcaaaggcag cttttgtcac cggaggagac cttggtctca gttttagagc tagaaatagc
     3541 aagttaaaat aaggctagtc cgttatcaac ttgaaaaagt ggcaccgagt cggtgctttt
     3601 gtcaccggat gtgttttccg gtctgatgag tccgtgagga cgaaacaaaa gctttctttt
     3661 cttttccttt tcgttgcttg ttttcccctt ctgatgttgt gtgttgagtt tattgtttgg
     3721 tttgctggta gatccagtat ttggatcatt tttccttttt tttttctttc attgcttctg
     3781 cggagggatg agtttgtgca tgaatctttt gttggttgga ttcgcttagt tttcacacta
     3841 gtcctttgat tcagcgcaaa tctttgttaa ttggctcgct taattctgac ataaagtgtt
     3901 ccttcttggt tctgcttgca ggtggtggat aagggtgcta gtgctcagtc tttcatcgag
     3961 aggatgacca acttcgataa gaaccttcca aacgagaagg tgctccctaa gcactctttg
     4021 ctctacgagt acttcaccgt gtacaacgag ttgaccaagg ttaagtacgt gaccgaggga
     4081 atgaggaagc ctgctttttt gtcaggtgag caaaagaagg ctatcgttga tctcttgttc
     4141 aagaccaaca gaaaggtgac cgtgaagcag ctcaaagagg attacttcaa gaaaatcgag
     4201 tgcttcgatt cagttgagat ttctggtgtt gaggataggt tcaacgcatc tctcggaacc
     4261 taccacgatc tcctcaagat cattaaggat aaggatttct tggataacga ggaaaacgag
     4321 gatatcttgg aggatatcgt tcttaccctc accctctttg aagatagaga gatgattgaa
     4381 gaaaggctca agacctacgc tcatctcttc gatgataagg tgatgaagca gttgaagaga
     4441 agaagataca ctggttgggg aaggctctca agaaagctca ttaacggaat cagggataag
     4501 cagtctggaa agacaatcct tgatttcctc aagtctgatg gattcgctaa cagaaacttc
     4561 atgcagctca tccacgatga ttctctcacc tttaaagagg atatccagaa ggctcaggtt
     4621 tcaggacagg gtgatagtct ccatgagcat atcgctaacc tcgctggatc tcctgcaatc
     4681 aagaagggaa tcctccagac tgtgaaggtt gtggatgagt tggtgaaggt gatgggaagg
     4741 cataagcctg agaacatcgt gatcgaaatg gctagagaga accagaccac tcagaaggga
     4801 cagaagaact ctagggaaag gatgaagagg atcgaggaag gtatcaaaga gcttggatct
     4861 cagatcctca aagagcaccc tgttgagaac actcagctcc agaatgagaa gctctacctc
     4921 tactacctcc agaacggaag ggatatgtat gtggatcaag agttggatat caacaggctc
     4981 tctgattacg atgttgatca tatcgtgcca cagtcattct tgaaggatga ttctatcgat
     5041 aacaaggtgc tcaccaggtc tgataagaac aggggtaaga gtgataacgt gccaagtgaa
     5101 gaggttgtga agaaaatgaa gaactattgg aggcagctcc tcaacgctaa gctcatcact
     5161 cagagaaagt tcgataactt gactaaggct gagaggggag gactctctga attggataag
     5221 gcaggattca tcaagaggca gcttgtggaa accaggcaga tcactaagca cgttgcacag
     5281 atcctcgatt ctaggatgaa caccaagtac gatgagaacg ataagttgat cagggaagtg
     5341 aaggttatca ccctcaagtc aaagctcgtg tctgatttca gaaaggattt ccaattctac
     5401 aaggtgaggg aaatcaacaa ctaccaccac gctcacgatg cttaccttaa cgctgttgtt
     5461 ggaaccgctc tcatcaagaa gtatcctaag ctcgagtcag agttcgtgta cggtgattac
     5521 aaggtgtacg atgtgaggaa gatgatcgct aagtctgagc aagagatcgg aaaggctacc
     5581 gctaagtatt tcttctactc taacatcatg aatttcttca agaccgagat taccctcgct
     5641 aacggtgaga tcagaaagag gccactcatc gagacaaacg gtgaaacagg tgagatcgtg
     5701 tgggataagg gaagggattt cgctaccgtt agaaaggtgc tctctatgcc acaggtgaac
     5761 atcgttaaga aaaccgaggt gcagaccggt ggattctcta aagagtctat cctccctaag
     5821 aggaactctg ataagctcat tgctaggaag aaggattggg accctaagaa atacggtggt
     5881 ttcgattctc ctaccgtggc ttactctgtt ctcgttgtgg ctaaggttga gaagggaaag
     5941 agtaagaagc tcaagtctgt taaggaactt ctcggaatca ctatcatgga aaggtcatct
     6001 ttcgagaaga acccaatcga tttcctcgag gctaagggat acaaagaggt taagaaggat
     6061 ctcatcatca agctcccaaa gtactcactc ttcgaactcg agaacggtag aaagaggatg
     6121 ctcgcttctg ctggtgagct tcaaaaggga aacgagcttg ctctcccatc taagtacgtt
     6181 aactttcttt acctcgcttc tcactacgag aagttgaagg gatctccaga agataacgag
     6241 cagaagcaac ttttcgttga gcagcacaag cactacttgg atgagatcat cgagcagatc
     6301 tctgagttct ctaaaagggt gatcctcgct gatgcaaacc tcgataaggt gttgtctgct
     6361 tacaacaagc acagagataa gcctatcagg gaacaggcag agaacatcat ccatctcttc
     6421 acccttacca acctcggtgc tcctgctgct ttcaagtact tcgatacaac catcgatagg
     6481 aagagataca cctctaccaa agaagtgctc gatgctaccc tcatccatca gtctatcact
     6541 ggactctacg agactaggat cgatctctca cagctcggtg gtgattcaag ggctgatcct
     6601 aagaagaaga ggaaggtttg actcgagata tgaagatgaa gatgaaatat ttggtgtgtc
     6661 aaataaaaag cttgtgtgct taagtttgtg tttttttctt ggcttgttgt gttatgaatt
     6721 tgtggctttt tctaatatta aatgaatgta agatcacatt ataatgaata aacaaatgtt
     6781 tctataatcc attgtgaatg ttttgttgga tctcttctgc agcatataac tactgtatgt
     6841 gctatggtat ggactatgga atatgattaa agataaggag ctcaaactat cagtgtttga
     6901 caggatatat tggcgggtaa acctaagaga aaagagcgtt tattagaata acggatattt
     6961 aaaagggcgt gaaaaggttt atccgttcgt ccatttgtat gtgcatgcca accacagggt
     7021 tcccctcggg atcaaagtac tttgatccaa cccctccgct gctatagtgc agtcggcttc
     7081 tgacgttcag tgcagccgtc ttctgaaaac gacatgtcgc acaagtccta agttacgcga
     7141 caggctgccg ccctgccctt ttcctggcgt tttcttgtcg cgtgttttag tcgcataaag
     7201 tagaatactt gcgactagaa ccggagacat tacgccatga acaagagcgc cgccgctggc
     7261 ctgctgggct atgcccgcgt cagcaccgac gaccaggact tgaccaacca acgggccgaa
     7321 ctgcacgcgg ccggctgcac caagctgttt tccgagaaga tcaccggcac caggcgcgac
     7381 cgcccggagc tggccaggat gcttgaccac ctacgccctg gcgacgttgt gacagtgacc
     7441 aggctagacc gcctggcccg cagcacccgc gacctactgg acattgccga gcgcatccag
     7501 gaggccggcg cgggcctgcg tagcctggca gagccgtggg ccgacaccac cacgccggcc
     7561 ggccgcatgg tgttgaccgt gttcgccggc attgccgagt tcgagcgttc cctaatcatc
     7621 gaccgcaccc ggagcgggcg cgaggccgcc aaggcccgag gcgtgaagtt tggcccccgc
     7681 cctaccctca ccccggcaca gatcgcgcac gcccgcgagc tgatcgacca ggaaggccgc
     7741 accgtgaaag aggcggctgc actgcttggc gtgcatcgct cgaccctgta ccgcgcactt
     7801 gagcgcagcg aggaagtgac gcccaccgag gccaggcggc gcggtgcctt ccgtgaggac
     7861 gcattgaccg aggccgacgc cctggcggcc gccgagaatg aacgccaaga ggaacaagca
     7921 tgaaaccgca ccaggacggc caggacgaac cgtttttcat taccgaagag atcgaggcgg
     7981 agatgatcgc ggccgggtac gtgttcgagc cgcccgcgca cggctcaacc gtgcggctgc
     8041 atgaaatcct ggccggtttg tctgatgcca agctggcggc ctggccggcc agcttggccg
     8101 ctgaagaaac cgagcgccgc cgtctaaaaa ggtgatgtgt atttgagtaa aacagcttgc
     8161 gtcatgcggt cgctgcgtat atgatgcgat gagtaaataa acaaatacgc aaggggaacg
     8221 catgaaggtt atcgctgtac ttaaccagaa aggcgggtca ggcaagacga ccatcgcaac
     8281 ccatctagcc cgcgccctgc aactcgccgg ggccgatgtt ctgttagtcg attccgatcc
     8341 ccagggcagt gcccgcgatt gggcggccgt gcgggaagat caaccgctaa ccgttgtcgg
     8401 catcgaccgc ccgacgattg accgcgacgt gaaggccatc ggccggcgcg acttcgtagt
     8461 gatcgacgga gcgccccagg cggcggactt ggctgtgtcc gcgatcaagg cagccgactt
     8521 cgtgctgatt ccggtgcagc caagccctta cgacatatgg gccaccgccg acctggtgga
     8581 gctggttaag cagcgcattg aggtcacgga tggaaggcta caagcggcct ttgtcgtgtc
     8641 gcgggcgatc aaaggcacgc gcatcggcgg tgaggttgcc gaggcgctgg ccgggtacga
     8701 gctgcccatt cttgagtccc gtatcacgca gcgcgtgagc tacccaggca ctgccgccgc
     8761 cggcacaacc gttcttgaat cagaacccga gggcgacgct gcccgcgagg tccaggcgct
     8821 ggccgctgaa attaaatcaa aactcatttg agttaatgag gtaaagagaa aatgagcaaa
     8881 agcacaaaca cgctaagtgc cggccgtccg agcgcacgca gcagcaaggc tgcaacgttg
     8941 gccagcctgg cagacacgcc agccatgaag cgggtcaact ttcagttgcc ggcggaggat
     9001 cacaccaagc tgaagatgta cgcggtacgc caaggcaaga ccattaccga gctgctatct
     9061 gaatacatcg cgcagctacc agagtaaatg agcaaatgaa taaatgagta gatgaatttt
     9121 agcggctaaa ggaggcggca tggaaaatca agaacaacca ggcaccgacg ccgtggaatg
     9181 ccccatgtgt ggaggaacgg gcggttggcc aggcgtaagc ggctgggttg tctgccggcc
     9241 ctgcaatggc actggaaccc ccaagcccga ggaatcggcg tgacggtcgc aaaccatccg
     9301 gcccggtaca aatcggcgcg gcgctgggtg atgacctggt ggagaagttg aaggccgcgc
     9361 aggccgccca gcggcaacgc atcgaggcag aagcacgccc cggtgaatcg tggcaagcgg
     9421 ccgctgatcg aatccgcaaa gaatcccggc aaccgccggc agccggtgcg ccgtcgatta
     9481 ggaagccgcc caagggcgac gagcaaccag attttttcgt tccgatgctc tatgacgtgg
     9541 gcacccgcga tagtcgcagc atcatggacg tggccgtttt ccgtctgtcg aagcgtgacc
     9601 gacgagctgg cgaggtgatc cgctacgagc ttccagacgg gcacgtagag gtttccgcag
     9661 ggccggccgg catggccagt gtgtgggatt acgacctggt actgatggcg gtttcccatc
     9721 taaccgaatc catgaaccga taccgggaag ggaagggaga caagcccggc cgcgtgttcc
     9781 gtccacacgt tgcggacgta ctcaagttct gccggcgagc cgatggcgga aagcagaaag
     9841 acgacctggt agaaacctgc attcggttaa acaccacgca cgttgccatg cagcgtacga
     9901 agaaggccaa gaacggccgc ctggtgacgg tatccgaggg tgaagccttg attagccgct
     9961 acaagatcgt aaagagcgaa accgggcggc cggagtacat cgagatcgag ctagctgatt
    10021 ggatgtaccg cgagatcaca gaaggcaaga acccggacgt gctgacggtt caccccgatt
    10081 actttttgat cgatcccggc atcggccgtt ttctctaccg cctggcacgc cgcgccgcag
    10141 gcaaggcaga agccagatgg ttgttcaaga cgatctacga acgcagtggc agcgccggag
    10201 agttcaagaa gttctgtttc accgtgcgca agctgatcgg gtcaaatgac ctgccggagt
    10261 acgatttgaa ggaggaggcg gggcaggctg gcccgatcct agtcatgcgc taccgcaacc
    10321 tgatcgaggg cgaagcatcc gccggttcct aatgtacgga gcagatgcta gggcaaattg
    10381 ccctagcagg ggaaaaaggt cgaaaaggcc tctttcctgt ggatagcacg tacattggga
    10441 acccaaagcc gtacattggg aaccggaacc cgtacattgg gaacccaaag ccgtacattg
    10501 ggaaccggtc acacatgtaa gtgactgata taaaagagaa aaaaggcgat ttttccgcct
    10561 aaaactcttt aaaacttatt aaaactctta aaacccgcct ggcctgtgca taactgtctg
    10621 gccagcgcac agccgaagag ctgcaaaaag cgcctaccct tcggtcgctg cgctccctac
    10681 gccccgccgc ttcgcgtcgg cctatcgcgg ccgctggccg ctcaaaaatg gctggcctac
    10741 ggccaggcaa tctaccaggg cgcggacaag ccgcgccgtc gccactcgac cgccggcgcc
    10801 cacatcaagg caccctgcct cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg
    10861 cagctcccgg aaacggtcac agcttgtctg taagcggatg ccgggagcag acaagcccgt
    10921 cagggcgcgt cagcgggtgt tggcgggtgt cggggcgcag ccatgaccca gtcacgtagc
    10981 gatagcggag tgtatactgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
    11041 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgct
    11101 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
    11161 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
    11221 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
    11281 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
    11341 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
    11401 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
    11461 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
    11521 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
    11581 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
    11641 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
    11701 ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta
    11761 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
    11821 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
    11881 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
    11941 tcatgcattc taggtactaa aacaattcat ccagtaaaat ataatatttt attttctccc
    12001 aatcaggctt gatccccagt aagtcaaaaa atagctcgac atactgttct tccccgatat
    12061 cctccctgat cgaccggacg cagaaggcaa tgtcatacca cttgtccgcc ctgccgcttc
    12121 tcccaagatc aataaagcca cttactttgc catctttcac aaagatgttg ctgtctccca
    12181 ggtcgccgtg ggaaaagaca agttcctctt cgggcttttc cgtctttaaa aaatcataca
    12241 gctcgcgcgg atctttaaat ggagtgtctt cttcccagtt ttcgcaatcc acatcggcca
    12301 gatcgttatt cagtaagtaa tccaattcgg ctaagcggct gtctaagcta ttcgtatagg
    12361 gacaatccga tatgtcgatg gagtgaaaga gcctgatgca ctccgcatac agctcgataa
    12421 tcttttcagg gctttgttca tcttcatact cttccgagca aaggacgcca tcggcctcac
    12481 tcatgagcag attgctccag ccatcatgcc gttcaaagtg caggaccttt ggaacaggca
    12541 gctttccttc cagccatagc atcatgtcct tttcccgttc cacatcatag gtggtccctt
    12601 tataccggct gtccgtcatt tttaaatata ggttttcatt ttctcccacc agcttatata
    12661 ccttagcagg agacattcct tccgtatctt ttacgcagcg gtatttttcg atcagttttt
    12721 tcaattccgg tgatattctc attttagcca tttattattt ccttcctctt ttctacagta
    12781 tttaaagata ccccaagaag ctaattataa caagacgaac tccaattcac tgttccttgc
    12841 attctaaaac cttaaatacc agaaaacagc tttttcaaag ttgttttcaa agttggcgta
    12901 taacatagta tcgacggagc cgattttgaa accgcggtga tcacaggcag caacgctctg
    12961 tcatcgttac aatcaacatg ctaccctccg cgagatcatc cgtgtttcaa acccggcagc
    13021 ttagttgccg ttcttccgaa tagcatcggt aacatgagca aagtctgccg ccttacaacg
    13081 gctctcccgc tgacgccgtc ccggactgat gggctgcctg tatcgagtgg tgattttgtg
    13141 ccgagctgcc ggtcggggag ctgttggctg gctggtggca ggatatattg tggtgtaaac
    13201 aaattgacgc ttagacaact taataacaca ttgcggacgt ttttaatgta ctgaattaac
    13261 gccgaattaa ttcgggggat ctggatttta gtactggatt ttggttttag gaattagaaa
    13321 ttttattgat agaagtattt tacaaataca aatacatact aagggtttct tatatgctca
    13381 acacatgagc gaaaccctat aggaacccta attcccttat ctgggaacta ctcacacatt
    13441 attatggaga aaatagagct tgtcgatcgt taattaatca cctccaagat cccggacgag
    13501 tgctggggcg tcggtttcca ctatcggcga gtacttctac acagccatcg gtccagacgg
    13561 ccgcgcttct gcgggcgatt tgtgtacgcc cgacagtccc ggctccggat cggacgattg
    13621 cgtcgcatcg accctgcgcc caagctgcat catcgaaatt gccgtcaacc aagctctgat
    13681 agagttggtc aagaccaatg cggagcatat acgcccggag ccgcggcgat cctgcaagct
    13741 ccggatgcct ccgctcgaag tagcgcgtct gctgctccat acaagccaac cacggcctcc
    13801 agaagaagat gttggcgacc tcgtattggg aatccccgaa catcgcctcg ctccagtcaa
    13861 tgaccgctgt tatgcggcca ttgtccgtca ggacattgtt ggagccgaaa tccgcgtgca
    13921 cgaggtgccg gacttcgggg cagtcctcgg cccaaagcat cagctcatcg agagcctgcg
    13981 cgacggacgc actgacggtg tcgtccatca cagtttgcca gtgatacaca tggggatcag
    14041 caatcgcgca tatgaaatca cgccatgtag tgtattgacc gattccttgc ggtccgaatg
    14101 ggccgaaccc gctcgtctgg ctaagatcgg ccgcagcgat cgcatccatg gcctccgcga
    14161 ccggctgcag aacagcgggc agttcggttt caggcaggtc ttgcaacgtg acaccctgtg
    14221 cacggcggga gatgcaatag gtcaggctct cgctgaattc cccaatgtca agcacttccg
    14281 gaatcgggag cgcggccgat gcaaagtgcc gataaacata acgatctttg tagaaaccat
    14341 cggcgcagct atttacccgc aggacatatc cacgccctcc tacatcgaag ctgaaagcac
    14401 gagattcttc gccctccgag agctgcatca ggtcggacac gctgtcgaac ttttcgatca
    14461 gaaacttctc gacagacgtc gcggtgagtt caggcttttt catcctagga gagatagatt
    14521 tgtagagaga gactggtgat ttcagcgtgt cctctccaaa tgaaatgaac ttccttatat
    14581 agaggaaggt cttgcgaagg atagtgggat tgtgcgtcat cccttacgtc agtggagata
    14641 tcacatcaat ccacttgctt tgaagacgtg gttggaacgt cttctttttc cacgatgctc
    14701 ctcgtgggtg ggggtccatc tttgggacca ctgtcggcag aggcatcttg aacgatagcc
    14761 tttcctttat cgcaatgatg gcatttgtag gtgccacctt ccttttctac tgtccttttg
    14821 atgaagtgac agatagctgg gcaatggaat ccgaggaggt ttcccgatat taccctttgt
    14881 tgaaaagtct caatagccct ttggtcttct gagactgtat ctttgatatt cttggagtag
    14941 acgagagtgt cgtgctccac catgttatca catcaatcca cttgctttga agacgtggtt
    15001 ggaacgtctt ctttttccac gatgctcctc gtgggtgggg gtccatcttt gggaccactg
    15061 tcggcagagg catcttgaac gatagccttt cctttatcgc aatgatggca tttgtaggtg
    15121 ccaccttcct tttctactgt ccttttgatg aagtgacaga tagctgggca atggaatccg
    15181 aggaggtttc ccgatattac cctttgttga aaagtctcaa tagccctttg gtcttctgag
    15241 actgtatctt tgatattctt ggagtagacg agagtgtcgt gctccaccat g
//
