LOCUS       sequence-266267-       15120 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  iSTU-CRISPR/Cas9 with RcCat intron, NU structure backbone.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15120)
  AUTHORS   Zhong Z, Liu S, Liu X, Liu B, Tang X, Ren Q, Zhou J, Zheng X, Qi Y, 
            Zhang Y
  TITLE     Intron-Based Single Transcript Unit CRISPR Systems for Plant Genome 
            Editing.
  JOURNAL   Rice (N Y). 2020 Feb 3;13(1):8. doi: 10.1186/s12284-020-0369-8.
  PUBMED    32016614
REFERENCE   2  (bases 1 to 15120)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Rice (N 
            Y)."; date: "2020-02-3"; volume: "13(1):8. doi"; pages: " 
            10.1186/s12284-020-0369-8"
COMMENT     Sequence Label: pGEL083
FEATURES             Location/Qualifiers
     source          1..15120
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        8..2004
                     /label=Ubi promoter
                     /note="maize polyubiquitin gene promoter"
     misc_RNA        3518..3593
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     CDS             4030..6414
                     /codon_start=1
                     /product="C-terminal portion of Streptococcus pyogenes Cas9
                     (Zetsche et al., 2015)"
                     /label=Cas9(C)
                     /translation="CFDSVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILED
                     IVLTLTLFEDREMIEERLKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGK
                     TILDFLKSDGFANRNFMQLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKG
                     ILQTVKVVDELVKVMGRHKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQI
                     LKEHPVENTQLQNEKLYLYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDN
                     KVLTRSDKNRGKSDNVPSEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDK
                     AGFIKRQLVETRQITKHVAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQF
                     YKVREINNYHHAHDAYLNAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGK
                     ATAKYFFYSNIMNFFKTEITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMP
                     QVNIVKKTEVQTGGFSKESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKV
                     EKGKSKKLKSVKELLGITIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELEN
                     GRKRMLASAGELQKGNELALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLD
                     EIIEQISEFSKRVILADANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKY
                     FDTTIDRKRYTSTKEVLDATLIHQSITGLYETRIDLSQLGGD"
     CDS             6427..6447
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     terminator      6458..6706
                     /label=HSP terminator
                     /note="efficient transcription terminator from the 
                     Arabidopsis thaliana heat shock protein 18.2 gene (Nagaya 
                     et al., 2010)"
     misc_feature    6727..6751
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             8051..8680
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             9114..10181
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQA
                     AADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKR
                     DRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGR
                     VFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEAL
                     ISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLA
                     RRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILV
                     MRYRNLIEGEASAGS"
     rep_origin      10247..10441
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(10677..10699)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    10785..10925
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     10840..10859
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(10940..10957)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(11111..11699)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11191..11210)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(11786..12580)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    13005..13029
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    13107..13281
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     promoter        complement(14371..15048)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
ORIGIN
        1 ggcgcgcctg cagtgcagcg tgacccggtc gtgcccctct cttgagataa tgagcattgc
       61 atgtctaagt tataaaaaat taccacatat tttttttgtc acacttgttt gaagtgcagt
      121 ttatctatct ttatacatat atttaaactt tactctacga ataatataat ctatagtact
      181 acaataatat cagtgtttta gagaatcata taaatgaaca gttagacatg gtctaaagga
      241 caattgagta ttttgacaac aggactctac agttttatct ttttagtgtg catgtgttct
      301 cctttttttt tgcaaatagc ttcacctata taatacttca tccattttat tagtacatcc
      361 atttagggtt tagggttaat ggtttttata gactaatttt tttagtacat ctattttatt
      421 ctattttagc ctctaaatta agaaaactaa aactctattt tagttttttt atttaataat
      481 ttagatataa aatagaataa aataaagtga ctaaaaatta aacaaatacc ctttaagaaa
      541 ttaaaaaaac taaggaaaca tttttcttgt ttcgagtaga taatgccagc ctgttaaacg
      601 ccgtcgacga gtctaacgga caccaaccag cgaaccagca gcgtcgcgtc gggccaagcg
      661 aagcagacgg cacggcatct ctgtcgctgc ctctggaccc ctctcgagag ttccgctcca
      721 ccgttggact tgctccgctg tcggcatcca gaaattgcgt ggcggagcgg cagacgtgag
      781 ccggcacggc aggcggcctc ctcctcctct cacggcaccg gcagctacgg gggattcctt
      841 tcccaccgct ccttcgcttt cccttcctcg cccgccgtaa taaatagaca ccccctccac
      901 accctctttc cccaacctcg tgttgttcgg agcgcacaca cacacaacca gaactccccc
      961 aaatccaccc gtcggcacct ccgcttcaag gtacgccgct cgtcctcccc ccccccccct
     1021 ctctaccttc tcaagatcgg cgttccggtc catggttagg gcccggtagt tctacttctg
     1081 ttcatgtttg tgttagatcc gtgtttgtgt tagatccgtg ctactagcgt tcgtacacgg
     1141 atgcgacctg tacgtcagac acgttctgat tgctaacttg ccagtgtttc tctttgggga
     1201 atcctgggat ggctctagcc gttccgcaga cgggatcgat ttcatgattt tttttgtttc
     1261 gttgcatagg gtttggtttg cccttttcct ttatttcaat atatgccgtg cacttgtttg
     1321 tcgggtcatc ttttcatgct tttttttgtc ttggttgtga tgatgtggtc tggttgggcg
     1381 gtcgttcaag atcggagtag aattaattct gtttcaaact acctggtgga tttattaatt
     1441 ttggatctgt atgtgtgtgc catacatatt catagttacg aattgaagat gatggatgga
     1501 aatatcgatc taggataggt atacatgttg atgcgggttt tactgatgca tatacagaga
     1561 tgctttttgt tcgcttggtt gtgatgatgt ggtgtggttg ggcggtcgtt cattcgttca
     1621 agatcggagt agaatactgt ttcaaactac ctggtgtatt tattaatttt ggaactgtat
     1681 gtgtgtgtca tacatcttca tagttacgag tttaagatgg atggaaatat cgatctagga
     1741 taggtataca tgttgatgtg ggttttactg atgcatatac atgatggcat atgcagcatc
     1801 tattcatatg ctctaacctt gagtacctat ctattataat aaacaagtat gttttataat
     1861 tattttgatc ttgatatact tggatgatgg catatgcagc agctatatgt ggattttttt
     1921 agccctgcct tcatacgcta tttatttgct tggtactgtt tcttttgtcg atgctcaccc
     1981 tgttgtttgg tgttacttct gcagcctgca ggtagatcgc tcgtcgacat ggataagaag
     2041 tactctatcg gactcgatat cggaactaac tctgtgggat gggctgtgat caccgatgag
     2101 tacaaggtgc catctaagaa gttcaaggtt ctcggaaaca ccgataggca ctctatcaag
     2161 aaaaacctta tcggtgctct cctcttcgat tctggtgaaa ctgctgaggc taccagactc
     2221 aagagaaccg ctagaagaag gtacaccaga agaaagaaca ggatctgcta cctccaagag
     2281 atcttctcta acgagatggc taaagtggat gattcattct tccacaggct cgaagagtca
     2341 ttcctcgtgg aagaagataa gaagcacgag aggcacccta tcttcggaaa catcgttgat
     2401 gaggtggcat accacgagaa gtaccctact atctaccacc tcagaaagaa gctcgttgat
     2461 tctactgata aggctgatct caggctcatc tacctcgctc tcgctcacat gatcaagttc
     2521 agaggacact tcctcatcga gggtgatctc aaccctgata actctgatgt ggataagttg
     2581 ttcatccagc tcgtgcagac ctacaaccag cttttcgaag agaaccctat caacgcttca
     2641 ggtgtggatg ctaaggctat cctctctgct aggctctcta agtcaagaag gcttgagaac
     2701 ctcattgctc agctccctgg tgagaagaag aacggacttt tcggaaactt gatcgctctc
     2761 tctctcggac tcacccctaa cttcaagtct aacttcgatc tcgctgagga tgcaaagctc
     2821 cagctctcaa aggataccta cgatgatgat ctcgataacc tcctcgctca gatcggagat
     2881 cagtacgctg atttgttcct cgctgctaag aacctctctg atgctatcct cctcagtgat
     2941 atcctcagag tgaacaccga gatcaccaag gctccactct cagcttctat gatcaagaga
     3001 tacgatgagc accaccagga tctcacactt ctcaaggctc ttgttagaca gcagctccca
     3061 gagaagtaca aagagatttt cttcgatcag tctaagaacg gatacgctgg ttacatcgat
     3121 ggtggtgcat ctcaagaaga gttctacaag ttcatcaagc ctatcctcga gaagatggat
     3181 ggaaccgagg aactcctcgt gaagctcaat agagaggatc ttctcagaaa gcagaggacc
     3241 ttcgataacg gatctatccc tcatcagatc cacctcggag agttgcacgc tatccttaga
     3301 aggcaagagg atttctaccc attcctcaag gataacaggg aaaagattga gaagattctc
     3361 accttcagaa tcccttacta cgtgggacct ctcgctagag gaaactcaag attcgcttgg
     3421 atgaccagaa agtctgagga aaccatcacc ccttggaact tcgaagaggt aaatttctag
     3481 tttttctcct tcattttctt gggagacctt ggtctcagtt ttagagctag aaatagcaag
     3541 ttaaaataag gctagtccgt tatcaacttg aaaaagtggc accgagtcgg tgcgttagga
     3601 cccttttctc tttttatttt tttgagcttt gatctttctt taaactgatc tattttttaa
     3661 ttgattggtt atggtgtaaa tattacatag ctttaactga taatctgatt actttatttc
     3721 gtgtgtctat gatgatgatg atagttacag gtggtggata agggtgctag tgctcagtct
     3781 ttcatcgaga ggatgaccaa cttcgataag aaccttccaa acgagaaggt gctccctaag
     3841 cactctttgc tctacgagta cttcaccgtg tacaacgagt tgaccaaggt taagtacgtg
     3901 accgagggaa tgaggaagcc tgcttttttg tcaggtgagc aaaagaaggc tatcgttgat
     3961 ctcttgttca agaccaacag aaaggtgacc gtgaagcagc tcaaagagga ttacttcaag
     4021 aaaatcgagt gcttcgattc agttgagatt tctggtgttg aggataggtt caacgcatct
     4081 ctcggaacct accacgatct cctcaagatc attaaggata aggatttctt ggataacgag
     4141 gaaaacgagg atatcttgga ggatatcgtt cttaccctca ccctctttga agatagagag
     4201 atgattgaag aaaggctcaa gacctacgct catctcttcg atgataaggt gatgaagcag
     4261 ttgaagagaa gaagatacac tggttgggga aggctctcaa gaaagctcat taacggaatc
     4321 agggataagc agtctggaaa gacaatcctt gatttcctca agtctgatgg attcgctaac
     4381 agaaacttca tgcagctcat ccacgatgat tctctcacct ttaaagagga tatccagaag
     4441 gctcaggttt caggacaggg tgatagtctc catgagcata tcgctaacct cgctggatct
     4501 cctgcaatca agaagggaat cctccagact gtgaaggttg tggatgagtt ggtgaaggtg
     4561 atgggaaggc ataagcctga gaacatcgtg atcgaaatgg ctagagagaa ccagaccact
     4621 cagaagggac agaagaactc tagggaaagg atgaagagga tcgaggaagg tatcaaagag
     4681 cttggatctc agatcctcaa agagcaccct gttgagaaca ctcagctcca gaatgagaag
     4741 ctctacctct actacctcca gaacggaagg gatatgtatg tggatcaaga gttggatatc
     4801 aacaggctct ctgattacga tgttgatcat atcgtgccac agtcattctt gaaggatgat
     4861 tctatcgata acaaggtgct caccaggtct gataagaaca ggggtaagag tgataacgtg
     4921 ccaagtgaag aggttgtgaa gaaaatgaag aactattgga ggcagctcct caacgctaag
     4981 ctcatcactc agagaaagtt cgataacttg actaaggctg agaggggagg actctctgaa
     5041 ttggataagg caggattcat caagaggcag cttgtggaaa ccaggcagat cactaagcac
     5101 gttgcacaga tcctcgattc taggatgaac accaagtacg atgagaacga taagttgatc
     5161 agggaagtga aggttatcac cctcaagtca aagctcgtgt ctgatttcag aaaggatttc
     5221 caattctaca aggtgaggga aatcaacaac taccaccacg ctcacgatgc ttaccttaac
     5281 gctgttgttg gaaccgctct catcaagaag tatcctaagc tcgagtcaga gttcgtgtac
     5341 ggtgattaca aggtgtacga tgtgaggaag atgatcgcta agtctgagca agagatcgga
     5401 aaggctaccg ctaagtattt cttctactct aacatcatga atttcttcaa gaccgagatt
     5461 accctcgcta acggtgagat cagaaagagg ccactcatcg agacaaacgg tgaaacaggt
     5521 gagatcgtgt gggataaggg aagggatttc gctaccgtta gaaaggtgct ctctatgcca
     5581 caggtgaaca tcgttaagaa aaccgaggtg cagaccggtg gattctctaa agagtctatc
     5641 ctccctaaga ggaactctga taagctcatt gctaggaaga aggattggga ccctaagaaa
     5701 tacggtggtt tcgattctcc taccgtggct tactctgttc tcgttgtggc taaggttgag
     5761 aagggaaaga gtaagaagct caagtctgtt aaggaacttc tcggaatcac tatcatggaa
     5821 aggtcatctt tcgagaagaa cccaatcgat ttcctcgagg ctaagggata caaagaggtt
     5881 aagaaggatc tcatcatcaa gctcccaaag tactcactct tcgaactcga gaacggtaga
     5941 aagaggatgc tcgcttctgc tggtgagctt caaaagggaa acgagcttgc tctcccatct
     6001 aagtacgtta actttcttta cctcgcttct cactacgaga agttgaaggg atctccagaa
     6061 gataacgagc agaagcaact tttcgttgag cagcacaagc actacttgga tgagatcatc
     6121 gagcagatct ctgagttctc taaaagggtg atcctcgctg atgcaaacct cgataaggtg
     6181 ttgtctgctt acaacaagca cagagataag cctatcaggg aacaggcaga gaacatcatc
     6241 catctcttca cccttaccaa cctcggtgct cctgctgctt tcaagtactt cgatacaacc
     6301 atcgatagga agagatacac ctctaccaaa gaagtgctcg atgctaccct catccatcag
     6361 tctatcactg gactctacga gactaggatc gatctctcac agctcggtgg tgattcaagg
     6421 gctgatccta agaagaagag gaaggtttga ctcgagatat gaagatgaag atgaaatatt
     6481 tggtgtgtca aataaaaagc ttgtgtgctt aagtttgtgt ttttttcttg gcttgttgtg
     6541 ttatgaattt gtggcttttt ctaatattaa atgaatgtaa gatcacatta taatgaataa
     6601 acaaatgttt ctataatcca ttgtgaatgt tttgttggat ctcttctgca gcatataact
     6661 actgtatgtg ctatggtatg gactatggaa tatgattaaa gataaggagc tcaaactatc
     6721 agtgtttgac aggatatatt ggcgggtaaa cctaagagaa aagagcgttt attagaataa
     6781 cggatattta aaagggcgtg aaaaggttta tccgttcgtc catttgtatg tgcatgccaa
     6841 ccacagggtt cccctcggga tcaaagtact ttgatccaac ccctccgctg ctatagtgca
     6901 gtcggcttct gacgttcagt gcagccgtct tctgaaaacg acatgtcgca caagtcctaa
     6961 gttacgcgac aggctgccgc cctgcccttt tcctggcgtt ttcttgtcgc gtgttttagt
     7021 cgcataaagt agaatacttg cgactagaac cggagacatt acgccatgaa caagagcgcc
     7081 gccgctggcc tgctgggcta tgcccgcgtc agcaccgacg accaggactt gaccaaccaa
     7141 cgggccgaac tgcacgcggc cggctgcacc aagctgtttt ccgagaagat caccggcacc
     7201 aggcgcgacc gcccggagct ggccaggatg cttgaccacc tacgccctgg cgacgttgtg
     7261 acagtgacca ggctagaccg cctggcccgc agcacccgcg acctactgga cattgccgag
     7321 cgcatccagg aggccggcgc gggcctgcgt agcctggcag agccgtgggc cgacaccacc
     7381 acgccggccg gccgcatggt gttgaccgtg ttcgccggca ttgccgagtt cgagcgttcc
     7441 ctaatcatcg accgcacccg gagcgggcgc gaggccgcca aggcccgagg cgtgaagttt
     7501 ggcccccgcc ctaccctcac cccggcacag atcgcgcacg cccgcgagct gatcgaccag
     7561 gaaggccgca ccgtgaaaga ggcggctgca ctgcttggcg tgcatcgctc gaccctgtac
     7621 cgcgcacttg agcgcagcga ggaagtgacg cccaccgagg ccaggcggcg cggtgccttc
     7681 cgtgaggacg cattgaccga ggccgacgcc ctggcggccg ccgagaatga acgccaagag
     7741 gaacaagcat gaaaccgcac caggacggcc aggacgaacc gtttttcatt accgaagaga
     7801 tcgaggcgga gatgatcgcg gccgggtacg tgttcgagcc gcccgcgcac ggctcaaccg
     7861 tgcggctgca tgaaatcctg gccggtttgt ctgatgccaa gctggcggcc tggccggcca
     7921 gcttggccgc tgaagaaacc gagcgccgcc gtctaaaaag gtgatgtgta tttgagtaaa
     7981 acagcttgcg tcatgcggtc gctgcgtata tgatgcgatg agtaaataaa caaatacgca
     8041 aggggaacgc atgaaggtta tcgctgtact taaccagaaa ggcgggtcag gcaagacgac
     8101 catcgcaacc catctagccc gcgccctgca actcgccggg gccgatgttc tgttagtcga
     8161 ttccgatccc cagggcagtg cccgcgattg ggcggccgtg cgggaagatc aaccgctaac
     8221 cgttgtcggc atcgaccgcc cgacgattga ccgcgacgtg aaggccatcg gccggcgcga
     8281 cttcgtagtg atcgacggag cgccccaggc ggcggacttg gctgtgtccg cgatcaaggc
     8341 agccgacttc gtgctgattc cggtgcagcc aagcccttac gacatatggg ccaccgccga
     8401 cctggtggag ctggttaagc agcgcattga ggtcacggat ggaaggctac aagcggcctt
     8461 tgtcgtgtcg cgggcgatca aaggcacgcg catcggcggt gaggttgccg aggcgctggc
     8521 cgggtacgag ctgcccattc ttgagtcccg tatcacgcag cgcgtgagct acccaggcac
     8581 tgccgccgcc ggcacaaccg ttcttgaatc agaacccgag ggcgacgctg cccgcgaggt
     8641 ccaggcgctg gccgctgaaa ttaaatcaaa actcatttga gttaatgagg taaagagaaa
     8701 atgagcaaaa gcacaaacac gctaagtgcc ggccgtccga gcgcacgcag cagcaaggct
     8761 gcaacgttgg ccagcctggc agacacgcca gccatgaagc gggtcaactt tcagttgccg
     8821 gcggaggatc acaccaagct gaagatgtac gcggtacgcc aaggcaagac cattaccgag
     8881 ctgctatctg aatacatcgc gcagctacca gagtaaatga gcaaatgaat aaatgagtag
     8941 atgaatttta gcggctaaag gaggcggcat ggaaaatcaa gaacaaccag gcaccgacgc
     9001 cgtggaatgc cccatgtgtg gaggaacggg cggttggcca ggcgtaagcg gctgggttgt
     9061 ctgccggccc tgcaatggca ctggaacccc caagcccgag gaatcggcgt gacggtcgca
     9121 aaccatccgg cccggtacaa atcggcgcgg cgctgggtga tgacctggtg gagaagttga
     9181 aggccgcgca ggccgcccag cggcaacgca tcgaggcaga agcacgcccc ggtgaatcgt
     9241 ggcaagcggc cgctgatcga atccgcaaag aatcccggca accgccggca gccggtgcgc
     9301 cgtcgattag gaagccgccc aagggcgacg agcaaccaga ttttttcgtt ccgatgctct
     9361 atgacgtggg cacccgcgat agtcgcagca tcatggacgt ggccgttttc cgtctgtcga
     9421 agcgtgaccg acgagctggc gaggtgatcc gctacgagct tccagacggg cacgtagagg
     9481 tttccgcagg gccggccggc atggccagtg tgtgggatta cgacctggta ctgatggcgg
     9541 tttcccatct aaccgaatcc atgaaccgat accgggaagg gaagggagac aagcccggcc
     9601 gcgtgttccg tccacacgtt gcggacgtac tcaagttctg ccggcgagcc gatggcggaa
     9661 agcagaaaga cgacctggta gaaacctgca ttcggttaaa caccacgcac gttgccatgc
     9721 agcgtacgaa gaaggccaag aacggccgcc tggtgacggt atccgagggt gaagccttga
     9781 ttagccgcta caagatcgta aagagcgaaa ccgggcggcc ggagtacatc gagatcgagc
     9841 tagctgattg gatgtaccgc gagatcacag aaggcaagaa cccggacgtg ctgacggttc
     9901 accccgatta ctttttgatc gatcccggca tcggccgttt tctctaccgc ctggcacgcc
     9961 gcgccgcagg caaggcagaa gccagatggt tgttcaagac gatctacgaa cgcagtggca
    10021 gcgccggaga gttcaagaag ttctgtttca ccgtgcgcaa gctgatcggg tcaaatgacc
    10081 tgccggagta cgatttgaag gaggaggcgg ggcaggctgg cccgatccta gtcatgcgct
    10141 accgcaacct gatcgagggc gaagcatccg ccggttccta atgtacggag cagatgctag
    10201 ggcaaattgc cctagcaggg gaaaaaggtc gaaaaggcct ctttcctgtg gatagcacgt
    10261 acattgggaa cccaaagccg tacattggga accggaaccc gtacattggg aacccaaagc
    10321 cgtacattgg gaaccggtca cacatgtaag tgactgatat aaaagagaaa aaaggcgatt
    10381 tttccgccta aaactcttta aaacttatta aaactcttaa aacccgcctg gcctgtgcat
    10441 aactgtctgg ccagcgcaca gccgaagagc tgcaaaaagc gcctaccctt cggtcgctgc
    10501 gctccctacg ccccgccgct tcgcgtcggc ctatcgcggc cgctggccgc tcaaaaatgg
    10561 ctggcctacg gccaggcaat ctaccagggc gcggacaagc cgcgccgtcg ccactcgacc
    10621 gccggcgccc acatcaaggc accctgcctc gcgcgtttcg gtgatgacgg tgaaaacctc
    10681 tgacacatgc agctcccgga aacggtcaca gcttgtctgt aagcggatgc cgggagcaga
    10741 caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc ggggcgcagc catgacccag
    10801 tcacgtagcg atagcggagt gtatactggc ttaactatgc ggcatcagag cagattgtac
    10861 tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga aaataccgca
    10921 tcaggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc
    10981 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg
    11041 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
    11101 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
    11161 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
    11221 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
    11281 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
    11341 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
    11401 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
    11461 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
    11521 agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga
    11581 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
    11641 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
    11701 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
    11761 ggattttggt catgcattct aggtactaaa acaattcatc cagtaaaata taatatttta
    11821 ttttctccca atcaggcttg atccccagta agtcaaaaaa tagctcgaca tactgttctt
    11881 ccccgatatc ctccctgatc gaccggacgc agaaggcaat gtcataccac ttgtccgccc
    11941 tgccgcttct cccaagatca ataaagccac ttactttgcc atctttcaca aagatgttgc
    12001 tgtctcccag gtcgccgtgg gaaaagacaa gttcctcttc gggcttttcc gtctttaaaa
    12061 aatcatacag ctcgcgcgga tctttaaatg gagtgtcttc ttcccagttt tcgcaatcca
    12121 catcggccag atcgttattc agtaagtaat ccaattcggc taagcggctg tctaagctat
    12181 tcgtataggg acaatccgat atgtcgatgg agtgaaagag cctgatgcac tccgcataca
    12241 gctcgataat cttttcaggg ctttgttcat cttcatactc ttccgagcaa aggacgccat
    12301 cggcctcact catgagcaga ttgctccagc catcatgccg ttcaaagtgc aggacctttg
    12361 gaacaggcag ctttccttcc agccatagca tcatgtcctt ttcccgttcc acatcatagg
    12421 tggtcccttt ataccggctg tccgtcattt ttaaatatag gttttcattt tctcccacca
    12481 gcttatatac cttagcagga gacattcctt ccgtatcttt tacgcagcgg tatttttcga
    12541 tcagtttttt caattccggt gatattctca ttttagccat ttattatttc cttcctcttt
    12601 tctacagtat ttaaagatac cccaagaagc taattataac aagacgaact ccaattcact
    12661 gttccttgca ttctaaaacc ttaaatacca gaaaacagct ttttcaaagt tgttttcaaa
    12721 gttggcgtat aacatagtat cgacggagcc gattttgaaa ccgcggtgat cacaggcagc
    12781 aacgctctgt catcgttaca atcaacatgc taccctccgc gagatcatcc gtgtttcaaa
    12841 cccggcagct tagttgccgt tcttccgaat agcatcggta acatgagcaa agtctgccgc
    12901 cttacaacgg ctctcccgct gacgccgtcc cggactgatg ggctgcctgt atcgagtggt
    12961 gattttgtgc cgagctgccg gtcggggagc tgttggctgg ctggtggcag gatatattgt
    13021 ggtgtaaaca aattgacgct tagacaactt aataacacat tgcggacgtt tttaatgtac
    13081 tgaattaacg ccgaattaat tcgggggatc tggattttag tactggattt tggttttagg
    13141 aattagaaat tttattgata gaagtatttt acaaatacaa atacatacta agggtttctt
    13201 atatgctcaa cacatgagcg aaaccctata ggaaccctaa ttcccttatc tgggaactac
    13261 tcacacatta ttatggagaa aatagagctt gtcgatcgtt aattaatcac ctccaagatc
    13321 ccggacgagt gctggggcgt cggtttccac tatcggcgag tacttctaca cagccatcgg
    13381 tccagacggc cgcgcttctg cgggcgattt gtgtacgccc gacagtcccg gctccggatc
    13441 ggacgattgc gtcgcatcga ccctgcgccc aagctgcatc atcgaaattg ccgtcaacca
    13501 agctctgata gagttggtca agaccaatgc ggagcatata cgcccggagc cgcggcgatc
    13561 ctgcaagctc cggatgcctc cgctcgaagt agcgcgtctg ctgctccata caagccaacc
    13621 acggcctcca gaagaagatg ttggcgacct cgtattggga atccccgaac atcgcctcgc
    13681 tccagtcaat gaccgctgtt atgcggccat tgtccgtcag gacattgttg gagccgaaat
    13741 ccgcgtgcac gaggtgccgg acttcggggc agtcctcggc ccaaagcatc agctcatcga
    13801 gagcctgcgc gacggacgca ctgacggtgt cgtccatcac agtttgccag tgatacacat
    13861 ggggatcagc aatcgcgcat atgaaatcac gccatgtagt gtattgaccg attccttgcg
    13921 gtccgaatgg gccgaacccg ctcgtctggc taagatcggc cgcagcgatc gcatccatgg
    13981 cctccgcgac cggctgcaga acagcgggca gttcggtttc aggcaggtct tgcaacgtga
    14041 caccctgtgc acggcgggag atgcaatagg tcaggctctc gctgaattcc ccaatgtcaa
    14101 gcacttccgg aatcgggagc gcggccgatg caaagtgccg ataaacataa cgatctttgt
    14161 agaaaccatc ggcgcagcta tttacccgca ggacatatcc acgccctcct acatcgaagc
    14221 tgaaagcacg agattcttcg ccctccgaga gctgcatcag gtcggacacg ctgtcgaact
    14281 tttcgatcag aaacttctcg acagacgtcg cggtgagttc aggctttttc atcctaggag
    14341 agatagattt gtagagagag actggtgatt tcagcgtgtc ctctccaaat gaaatgaact
    14401 tccttatata gaggaaggtc ttgcgaagga tagtgggatt gtgcgtcatc ccttacgtca
    14461 gtggagatat cacatcaatc cacttgcttt gaagacgtgg ttggaacgtc ttctttttcc
    14521 acgatgctcc tcgtgggtgg gggtccatct ttgggaccac tgtcggcaga ggcatcttga
    14581 acgatagcct ttcctttatc gcaatgatgg catttgtagg tgccaccttc cttttctact
    14641 gtccttttga tgaagtgaca gatagctggg caatggaatc cgaggaggtt tcccgatatt
    14701 accctttgtt gaaaagtctc aatagccctt tggtcttctg agactgtatc tttgatattc
    14761 ttggagtaga cgagagtgtc gtgctccacc atgttatcac atcaatccac ttgctttgaa
    14821 gacgtggttg gaacgtcttc tttttccacg atgctcctcg tgggtggggg tccatctttg
    14881 ggaccactgt cggcagaggc atcttgaacg atagcctttc ctttatcgca atgatggcat
    14941 ttgtaggtgc caccttcctt ttctactgtc cttttgatga agtgacagat agctgggcaa
    15001 tggaatccga ggaggtttcc cgatattacc ctttgttgaa aagtctcaat agccctttgg
    15061 tcttctgaga ctgtatcttt gatattcttg gagtagacga gagtgtcgtg ctccaccatg
//
