LOCUS       sequence-266268-       15897 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  iSTU-CRISPR/Cas9 with RcCat intron, tRNA structure backbone.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15897)
  AUTHORS   Zhong Z, Liu S, Liu X, Liu B, Tang X, Ren Q, Zhou J, Zheng X, Qi Y, 
            Zhang Y
  TITLE     Intron-Based Single Transcript Unit CRISPR Systems for Plant Genome 
            Editing.
  JOURNAL   Rice (N Y). 2020 Feb 3;13(1):8. doi: 10.1186/s12284-020-0369-8.
  PUBMED    32016614
REFERENCE   2  (bases 1 to 15897)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Rice (N 
            Y)."; date: "2020-02-3"; volume: "13(1):8. doi"; pages: " 
            10.1186/s12284-020-0369-8"
COMMENT     Sequence Label: pGEL085
FEATURES             Location/Qualifiers
     source          1..15897
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        8..2004
                     /label=Ubi promoter
                     /note="maize polyubiquitin gene promoter"
     CDS             complement(3586..3891)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(3688..3707)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     misc_RNA        4218..4293
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     CDS             4807..7191
                     /codon_start=1
                     /product="C-terminal portion of Streptococcus pyogenes Cas9
                     (Zetsche et al., 2015)"
                     /label=Cas9(C)
                     /translation="CFDSVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILED
                     IVLTLTLFEDREMIEERLKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGK
                     TILDFLKSDGFANRNFMQLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKG
                     ILQTVKVVDELVKVMGRHKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQI
                     LKEHPVENTQLQNEKLYLYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDN
                     KVLTRSDKNRGKSDNVPSEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDK
                     AGFIKRQLVETRQITKHVAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQF
                     YKVREINNYHHAHDAYLNAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGK
                     ATAKYFFYSNIMNFFKTEITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMP
                     QVNIVKKTEVQTGGFSKESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKV
                     EKGKSKKLKSVKELLGITIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELEN
                     GRKRMLASAGELQKGNELALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLD
                     EIIEQISEFSKRVILADANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKY
                     FDTTIDRKRYTSTKEVLDATLIHQSITGLYETRIDLSQLGGD"
     CDS             7204..7224
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     terminator      7235..7483
                     /label=HSP terminator
                     /note="efficient transcription terminator from the 
                     Arabidopsis thaliana heat shock protein 18.2 gene (Nagaya 
                     et al., 2010)"
     misc_feature    7504..7528
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             8828..9457
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             9891..10958
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQA
                     AADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKR
                     DRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGR
                     VFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEAL
                     ISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLA
                     RRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILV
                     MRYRNLIEGEASAGS"
     rep_origin      11024..11218
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(11454..11476)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    11562..11702
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     11617..11636
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(11717..11734)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(11888..12476)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11968..11987)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(12563..13357)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    13782..13806
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    13884..14058
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     promoter        complement(15148..15825)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
ORIGIN
        1 ggcgcgcctg cagtgcagcg tgacccggtc gtgcccctct cttgagataa tgagcattgc
       61 atgtctaagt tataaaaaat taccacatat tttttttgtc acacttgttt gaagtgcagt
      121 ttatctatct ttatacatat atttaaactt tactctacga ataatataat ctatagtact
      181 acaataatat cagtgtttta gagaatcata taaatgaaca gttagacatg gtctaaagga
      241 caattgagta ttttgacaac aggactctac agttttatct ttttagtgtg catgtgttct
      301 cctttttttt tgcaaatagc ttcacctata taatacttca tccattttat tagtacatcc
      361 atttagggtt tagggttaat ggtttttata gactaatttt tttagtacat ctattttatt
      421 ctattttagc ctctaaatta agaaaactaa aactctattt tagttttttt atttaataat
      481 ttagatataa aatagaataa aataaagtga ctaaaaatta aacaaatacc ctttaagaaa
      541 ttaaaaaaac taaggaaaca tttttcttgt ttcgagtaga taatgccagc ctgttaaacg
      601 ccgtcgacga gtctaacgga caccaaccag cgaaccagca gcgtcgcgtc gggccaagcg
      661 aagcagacgg cacggcatct ctgtcgctgc ctctggaccc ctctcgagag ttccgctcca
      721 ccgttggact tgctccgctg tcggcatcca gaaattgcgt ggcggagcgg cagacgtgag
      781 ccggcacggc aggcggcctc ctcctcctct cacggcaccg gcagctacgg gggattcctt
      841 tcccaccgct ccttcgcttt cccttcctcg cccgccgtaa taaatagaca ccccctccac
      901 accctctttc cccaacctcg tgttgttcgg agcgcacaca cacacaacca gaactccccc
      961 aaatccaccc gtcggcacct ccgcttcaag gtacgccgct cgtcctcccc ccccccccct
     1021 ctctaccttc tcaagatcgg cgttccggtc catggttagg gcccggtagt tctacttctg
     1081 ttcatgtttg tgttagatcc gtgtttgtgt tagatccgtg ctactagcgt tcgtacacgg
     1141 atgcgacctg tacgtcagac acgttctgat tgctaacttg ccagtgtttc tctttgggga
     1201 atcctgggat ggctctagcc gttccgcaga cgggatcgat ttcatgattt tttttgtttc
     1261 gttgcatagg gtttggtttg cccttttcct ttatttcaat atatgccgtg cacttgtttg
     1321 tcgggtcatc ttttcatgct tttttttgtc ttggttgtga tgatgtggtc tggttgggcg
     1381 gtcgttcaag atcggagtag aattaattct gtttcaaact acctggtgga tttattaatt
     1441 ttggatctgt atgtgtgtgc catacatatt catagttacg aattgaagat gatggatgga
     1501 aatatcgatc taggataggt atacatgttg atgcgggttt tactgatgca tatacagaga
     1561 tgctttttgt tcgcttggtt gtgatgatgt ggtgtggttg ggcggtcgtt cattcgttca
     1621 agatcggagt agaatactgt ttcaaactac ctggtgtatt tattaatttt ggaactgtat
     1681 gtgtgtgtca tacatcttca tagttacgag tttaagatgg atggaaatat cgatctagga
     1741 taggtataca tgttgatgtg ggttttactg atgcatatac atgatggcat atgcagcatc
     1801 tattcatatg ctctaacctt gagtacctat ctattataat aaacaagtat gttttataat
     1861 tattttgatc ttgatatact tggatgatgg catatgcagc agctatatgt ggattttttt
     1921 agccctgcct tcatacgcta tttatttgct tggtactgtt tcttttgtcg atgctcaccc
     1981 tgttgtttgg tgttacttct gcagcctgca ggtagatcgc tcgtcgacat ggataagaag
     2041 tactctatcg gactcgatat cggaactaac tctgtgggat gggctgtgat caccgatgag
     2101 tacaaggtgc catctaagaa gttcaaggtt ctcggaaaca ccgataggca ctctatcaag
     2161 aaaaacctta tcggtgctct cctcttcgat tctggtgaaa ctgctgaggc taccagactc
     2221 aagagaaccg ctagaagaag gtacaccaga agaaagaaca ggatctgcta cctccaagag
     2281 atcttctcta acgagatggc taaagtggat gattcattct tccacaggct cgaagagtca
     2341 ttcctcgtgg aagaagataa gaagcacgag aggcacccta tcttcggaaa catcgttgat
     2401 gaggtggcat accacgagaa gtaccctact atctaccacc tcagaaagaa gctcgttgat
     2461 tctactgata aggctgatct caggctcatc tacctcgctc tcgctcacat gatcaagttc
     2521 agaggacact tcctcatcga gggtgatctc aaccctgata actctgatgt ggataagttg
     2581 ttcatccagc tcgtgcagac ctacaaccag cttttcgaag agaaccctat caacgcttca
     2641 ggtgtggatg ctaaggctat cctctctgct aggctctcta agtcaagaag gcttgagaac
     2701 ctcattgctc agctccctgg tgagaagaag aacggacttt tcggaaactt gatcgctctc
     2761 tctctcggac tcacccctaa cttcaagtct aacttcgatc tcgctgagga tgcaaagctc
     2821 cagctctcaa aggataccta cgatgatgat ctcgataacc tcctcgctca gatcggagat
     2881 cagtacgctg atttgttcct cgctgctaag aacctctctg atgctatcct cctcagtgat
     2941 atcctcagag tgaacaccga gatcaccaag gctccactct cagcttctat gatcaagaga
     3001 tacgatgagc accaccagga tctcacactt ctcaaggctc ttgttagaca gcagctccca
     3061 gagaagtaca aagagatttt cttcgatcag tctaagaacg gatacgctgg ttacatcgat
     3121 ggtggtgcat ctcaagaaga gttctacaag ttcatcaagc ctatcctcga gaagatggat
     3181 ggaaccgagg aactcctcgt gaagctcaat agagaggatc ttctcagaaa gcagaggacc
     3241 ttcgataacg gatctatccc tcatcagatc cacctcggag agttgcacgc tatccttaga
     3301 aggcaagagg atttctaccc attcctcaag gataacaggg aaaagattga gaagattctc
     3361 accttcagaa tcccttacta cgtgggacct ctcgctagag gaaactcaag attcgcttgg
     3421 atgaccagaa agtctgagga aaccatcacc ccttggaact tcgaagaggt aaatttctag
     3481 tttttctcct tcattttctt gaacaaagca ccagtggtct agtggtagaa tagtaccctg
     3541 ccacggtaca gacccgggtt cgattcccgg ctggtgcagg agaccttata ttccccagaa
     3601 catcaggtta atggcgtttt tgatgtcatt ttcgcggtgg ctgagatcag ccacttcttc
     3661 cccgataacg gaaaccggca cactggccat atcggtggtc atcatgcgcc agctttcatc
     3721 cccgatatgc accaccgggt aaagttcacg ggagacttta tctgacagca gacgtgcact
     3781 ggccaggggg atcaccatcc gtcgcccggg cgtgtcaata atatcactct gtacatccac
     3841 aaacagacga taacggctct ctcttttata ggtgtaaacc ttaaactgca tttcaccagc
     3901 ccctgttctc gtcagcaaaa gagccgttca tttcaataaa ccgggcgacc tcagccatcc
     3961 cttcctgatt ttccgctttc cagcgttcgg cacgcagacg acgggcttca ttctgcatgg
     4021 ttgtgcttac cagaccggag atattgacat catatatgcc ttgagcaact gatagctgtc
     4081 gctgtcaact gtcactgtaa tacgctgctt catagcatac ctctttttga catacttcgg
     4141 gtatacatat cagtatatat tcttataccg caaaaatcag cgcgcaaata cgcatactgt
     4201 tatctggctt ggtctcagtt ttagagctag aaatagcaag ttaaaataag gctagtccgt
     4261 tatcaacttg aaaaagtggc accgagtcgg tgcaacaaag caccagtggt ctagtggtag
     4321 aatagtaccc tgccacggta cagacccggg ttcgattccc ggctggtgca gttaggaccc
     4381 ttttctcttt ttattttttt gagctttgat ctttctttaa actgatctat tttttaattg
     4441 attggttatg gtgtaaatat tacatagctt taactgataa tctgattact ttatttcgtg
     4501 tgtctatgat gatgatgata gttacaggtg gtggataagg gtgctagtgc tcagtctttc
     4561 atcgagagga tgaccaactt cgataagaac cttccaaacg agaaggtgct ccctaagcac
     4621 tctttgctct acgagtactt caccgtgtac aacgagttga ccaaggttaa gtacgtgacc
     4681 gagggaatga ggaagcctgc ttttttgtca ggtgagcaaa agaaggctat cgttgatctc
     4741 ttgttcaaga ccaacagaaa ggtgaccgtg aagcagctca aagaggatta cttcaagaaa
     4801 atcgagtgct tcgattcagt tgagatttct ggtgttgagg ataggttcaa cgcatctctc
     4861 ggaacctacc acgatctcct caagatcatt aaggataagg atttcttgga taacgaggaa
     4921 aacgaggata tcttggagga tatcgttctt accctcaccc tctttgaaga tagagagatg
     4981 attgaagaaa ggctcaagac ctacgctcat ctcttcgatg ataaggtgat gaagcagttg
     5041 aagagaagaa gatacactgg ttggggaagg ctctcaagaa agctcattaa cggaatcagg
     5101 gataagcagt ctggaaagac aatccttgat ttcctcaagt ctgatggatt cgctaacaga
     5161 aacttcatgc agctcatcca cgatgattct ctcaccttta aagaggatat ccagaaggct
     5221 caggtttcag gacagggtga tagtctccat gagcatatcg ctaacctcgc tggatctcct
     5281 gcaatcaaga agggaatcct ccagactgtg aaggttgtgg atgagttggt gaaggtgatg
     5341 ggaaggcata agcctgagaa catcgtgatc gaaatggcta gagagaacca gaccactcag
     5401 aagggacaga agaactctag ggaaaggatg aagaggatcg aggaaggtat caaagagctt
     5461 ggatctcaga tcctcaaaga gcaccctgtt gagaacactc agctccagaa tgagaagctc
     5521 tacctctact acctccagaa cggaagggat atgtatgtgg atcaagagtt ggatatcaac
     5581 aggctctctg attacgatgt tgatcatatc gtgccacagt cattcttgaa ggatgattct
     5641 atcgataaca aggtgctcac caggtctgat aagaacaggg gtaagagtga taacgtgcca
     5701 agtgaagagg ttgtgaagaa aatgaagaac tattggaggc agctcctcaa cgctaagctc
     5761 atcactcaga gaaagttcga taacttgact aaggctgaga ggggaggact ctctgaattg
     5821 gataaggcag gattcatcaa gaggcagctt gtggaaacca ggcagatcac taagcacgtt
     5881 gcacagatcc tcgattctag gatgaacacc aagtacgatg agaacgataa gttgatcagg
     5941 gaagtgaagg ttatcaccct caagtcaaag ctcgtgtctg atttcagaaa ggatttccaa
     6001 ttctacaagg tgagggaaat caacaactac caccacgctc acgatgctta ccttaacgct
     6061 gttgttggaa ccgctctcat caagaagtat cctaagctcg agtcagagtt cgtgtacggt
     6121 gattacaagg tgtacgatgt gaggaagatg atcgctaagt ctgagcaaga gatcggaaag
     6181 gctaccgcta agtatttctt ctactctaac atcatgaatt tcttcaagac cgagattacc
     6241 ctcgctaacg gtgagatcag aaagaggcca ctcatcgaga caaacggtga aacaggtgag
     6301 atcgtgtggg ataagggaag ggatttcgct accgttagaa aggtgctctc tatgccacag
     6361 gtgaacatcg ttaagaaaac cgaggtgcag accggtggat tctctaaaga gtctatcctc
     6421 cctaagagga actctgataa gctcattgct aggaagaagg attgggaccc taagaaatac
     6481 ggtggtttcg attctcctac cgtggcttac tctgttctcg ttgtggctaa ggttgagaag
     6541 ggaaagagta agaagctcaa gtctgttaag gaacttctcg gaatcactat catggaaagg
     6601 tcatctttcg agaagaaccc aatcgatttc ctcgaggcta agggatacaa agaggttaag
     6661 aaggatctca tcatcaagct cccaaagtac tcactcttcg aactcgagaa cggtagaaag
     6721 aggatgctcg cttctgctgg tgagcttcaa aagggaaacg agcttgctct cccatctaag
     6781 tacgttaact ttctttacct cgcttctcac tacgagaagt tgaagggatc tccagaagat
     6841 aacgagcaga agcaactttt cgttgagcag cacaagcact acttggatga gatcatcgag
     6901 cagatctctg agttctctaa aagggtgatc ctcgctgatg caaacctcga taaggtgttg
     6961 tctgcttaca acaagcacag agataagcct atcagggaac aggcagagaa catcatccat
     7021 ctcttcaccc ttaccaacct cggtgctcct gctgctttca agtacttcga tacaaccatc
     7081 gataggaaga gatacacctc taccaaagaa gtgctcgatg ctaccctcat ccatcagtct
     7141 atcactggac tctacgagac taggatcgat ctctcacagc tcggtggtga ttcaagggct
     7201 gatcctaaga agaagaggaa ggtttgactc gagatatgaa gatgaagatg aaatatttgg
     7261 tgtgtcaaat aaaaagcttg tgtgcttaag tttgtgtttt tttcttggct tgttgtgtta
     7321 tgaatttgtg gctttttcta atattaaatg aatgtaagat cacattataa tgaataaaca
     7381 aatgtttcta taatccattg tgaatgtttt gttggatctc ttctgcagca tataactact
     7441 gtatgtgcta tggtatggac tatggaatat gattaaagat aaggagctca aactatcagt
     7501 gtttgacagg atatattggc gggtaaacct aagagaaaag agcgtttatt agaataacgg
     7561 atatttaaaa gggcgtgaaa aggtttatcc gttcgtccat ttgtatgtgc atgccaacca
     7621 cagggttccc ctcgggatca aagtactttg atccaacccc tccgctgcta tagtgcagtc
     7681 ggcttctgac gttcagtgca gccgtcttct gaaaacgaca tgtcgcacaa gtcctaagtt
     7741 acgcgacagg ctgccgccct gcccttttcc tggcgttttc ttgtcgcgtg ttttagtcgc
     7801 ataaagtaga atacttgcga ctagaaccgg agacattacg ccatgaacaa gagcgccgcc
     7861 gctggcctgc tgggctatgc ccgcgtcagc accgacgacc aggacttgac caaccaacgg
     7921 gccgaactgc acgcggccgg ctgcaccaag ctgttttccg agaagatcac cggcaccagg
     7981 cgcgaccgcc cggagctggc caggatgctt gaccacctac gccctggcga cgttgtgaca
     8041 gtgaccaggc tagaccgcct ggcccgcagc acccgcgacc tactggacat tgccgagcgc
     8101 atccaggagg ccggcgcggg cctgcgtagc ctggcagagc cgtgggccga caccaccacg
     8161 ccggccggcc gcatggtgtt gaccgtgttc gccggcattg ccgagttcga gcgttcccta
     8221 atcatcgacc gcacccggag cgggcgcgag gccgccaagg cccgaggcgt gaagtttggc
     8281 ccccgcccta ccctcacccc ggcacagatc gcgcacgccc gcgagctgat cgaccaggaa
     8341 ggccgcaccg tgaaagaggc ggctgcactg cttggcgtgc atcgctcgac cctgtaccgc
     8401 gcacttgagc gcagcgagga agtgacgccc accgaggcca ggcggcgcgg tgccttccgt
     8461 gaggacgcat tgaccgaggc cgacgccctg gcggccgccg agaatgaacg ccaagaggaa
     8521 caagcatgaa accgcaccag gacggccagg acgaaccgtt tttcattacc gaagagatcg
     8581 aggcggagat gatcgcggcc gggtacgtgt tcgagccgcc cgcgcacggc tcaaccgtgc
     8641 ggctgcatga aatcctggcc ggtttgtctg atgccaagct ggcggcctgg ccggccagct
     8701 tggccgctga agaaaccgag cgccgccgtc taaaaaggtg atgtgtattt gagtaaaaca
     8761 gcttgcgtca tgcggtcgct gcgtatatga tgcgatgagt aaataaacaa atacgcaagg
     8821 ggaacgcatg aaggttatcg ctgtacttaa ccagaaaggc gggtcaggca agacgaccat
     8881 cgcaacccat ctagcccgcg ccctgcaact cgccggggcc gatgttctgt tagtcgattc
     8941 cgatccccag ggcagtgccc gcgattgggc ggccgtgcgg gaagatcaac cgctaaccgt
     9001 tgtcggcatc gaccgcccga cgattgaccg cgacgtgaag gccatcggcc ggcgcgactt
     9061 cgtagtgatc gacggagcgc cccaggcggc ggacttggct gtgtccgcga tcaaggcagc
     9121 cgacttcgtg ctgattccgg tgcagccaag cccttacgac atatgggcca ccgccgacct
     9181 ggtggagctg gttaagcagc gcattgaggt cacggatgga aggctacaag cggcctttgt
     9241 cgtgtcgcgg gcgatcaaag gcacgcgcat cggcggtgag gttgccgagg cgctggccgg
     9301 gtacgagctg cccattcttg agtcccgtat cacgcagcgc gtgagctacc caggcactgc
     9361 cgccgccggc acaaccgttc ttgaatcaga acccgagggc gacgctgccc gcgaggtcca
     9421 ggcgctggcc gctgaaatta aatcaaaact catttgagtt aatgaggtaa agagaaaatg
     9481 agcaaaagca caaacacgct aagtgccggc cgtccgagcg cacgcagcag caaggctgca
     9541 acgttggcca gcctggcaga cacgccagcc atgaagcggg tcaactttca gttgccggcg
     9601 gaggatcaca ccaagctgaa gatgtacgcg gtacgccaag gcaagaccat taccgagctg
     9661 ctatctgaat acatcgcgca gctaccagag taaatgagca aatgaataaa tgagtagatg
     9721 aattttagcg gctaaaggag gcggcatgga aaatcaagaa caaccaggca ccgacgccgt
     9781 ggaatgcccc atgtgtggag gaacgggcgg ttggccaggc gtaagcggct gggttgtctg
     9841 ccggccctgc aatggcactg gaacccccaa gcccgaggaa tcggcgtgac ggtcgcaaac
     9901 catccggccc ggtacaaatc ggcgcggcgc tgggtgatga cctggtggag aagttgaagg
     9961 ccgcgcaggc cgcccagcgg caacgcatcg aggcagaagc acgccccggt gaatcgtggc
    10021 aagcggccgc tgatcgaatc cgcaaagaat cccggcaacc gccggcagcc ggtgcgccgt
    10081 cgattaggaa gccgcccaag ggcgacgagc aaccagattt tttcgttccg atgctctatg
    10141 acgtgggcac ccgcgatagt cgcagcatca tggacgtggc cgttttccgt ctgtcgaagc
    10201 gtgaccgacg agctggcgag gtgatccgct acgagcttcc agacgggcac gtagaggttt
    10261 ccgcagggcc ggccggcatg gccagtgtgt gggattacga cctggtactg atggcggttt
    10321 cccatctaac cgaatccatg aaccgatacc gggaagggaa gggagacaag cccggccgcg
    10381 tgttccgtcc acacgttgcg gacgtactca agttctgccg gcgagccgat ggcggaaagc
    10441 agaaagacga cctggtagaa acctgcattc ggttaaacac cacgcacgtt gccatgcagc
    10501 gtacgaagaa ggccaagaac ggccgcctgg tgacggtatc cgagggtgaa gccttgatta
    10561 gccgctacaa gatcgtaaag agcgaaaccg ggcggccgga gtacatcgag atcgagctag
    10621 ctgattggat gtaccgcgag atcacagaag gcaagaaccc ggacgtgctg acggttcacc
    10681 ccgattactt tttgatcgat cccggcatcg gccgttttct ctaccgcctg gcacgccgcg
    10741 ccgcaggcaa ggcagaagcc agatggttgt tcaagacgat ctacgaacgc agtggcagcg
    10801 ccggagagtt caagaagttc tgtttcaccg tgcgcaagct gatcgggtca aatgacctgc
    10861 cggagtacga tttgaaggag gaggcggggc aggctggccc gatcctagtc atgcgctacc
    10921 gcaacctgat cgagggcgaa gcatccgccg gttcctaatg tacggagcag atgctagggc
    10981 aaattgccct agcaggggaa aaaggtcgaa aaggcctctt tcctgtggat agcacgtaca
    11041 ttgggaaccc aaagccgtac attgggaacc ggaacccgta cattgggaac ccaaagccgt
    11101 acattgggaa ccggtcacac atgtaagtga ctgatataaa agagaaaaaa ggcgattttt
    11161 ccgcctaaaa ctctttaaaa cttattaaaa ctcttaaaac ccgcctggcc tgtgcataac
    11221 tgtctggcca gcgcacagcc gaagagctgc aaaaagcgcc tacccttcgg tcgctgcgct
    11281 ccctacgccc cgccgcttcg cgtcggccta tcgcggccgc tggccgctca aaaatggctg
    11341 gcctacggcc aggcaatcta ccagggcgcg gacaagccgc gccgtcgcca ctcgaccgcc
    11401 ggcgcccaca tcaaggcacc ctgcctcgcg cgtttcggtg atgacggtga aaacctctga
    11461 cacatgcagc tcccggaaac ggtcacagct tgtctgtaag cggatgccgg gagcagacaa
    11521 gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg gcgcagccat gacccagtca
    11581 cgtagcgata gcggagtgta tactggctta actatgcggc atcagagcag attgtactga
    11641 gagtgcacca tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa taccgcatca
    11701 ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag
    11761 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag
    11821 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
    11881 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
    11941 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
    12001 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
    12061 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
    12121 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
    12181 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
    12241 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
    12301 ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc
    12361 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
    12421 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
    12481 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
    12541 ttttggtcat gcattctagg tactaaaaca attcatccag taaaatataa tattttattt
    12601 tctcccaatc aggcttgatc cccagtaagt caaaaaatag ctcgacatac tgttcttccc
    12661 cgatatcctc cctgatcgac cggacgcaga aggcaatgtc ataccacttg tccgccctgc
    12721 cgcttctccc aagatcaata aagccactta ctttgccatc tttcacaaag atgttgctgt
    12781 ctcccaggtc gccgtgggaa aagacaagtt cctcttcggg cttttccgtc tttaaaaaat
    12841 catacagctc gcgcggatct ttaaatggag tgtcttcttc ccagttttcg caatccacat
    12901 cggccagatc gttattcagt aagtaatcca attcggctaa gcggctgtct aagctattcg
    12961 tatagggaca atccgatatg tcgatggagt gaaagagcct gatgcactcc gcatacagct
    13021 cgataatctt ttcagggctt tgttcatctt catactcttc cgagcaaagg acgccatcgg
    13081 cctcactcat gagcagattg ctccagccat catgccgttc aaagtgcagg acctttggaa
    13141 caggcagctt tccttccagc catagcatca tgtccttttc ccgttccaca tcataggtgg
    13201 tccctttata ccggctgtcc gtcattttta aatataggtt ttcattttct cccaccagct
    13261 tatatacctt agcaggagac attccttccg tatcttttac gcagcggtat ttttcgatca
    13321 gttttttcaa ttccggtgat attctcattt tagccattta ttatttcctt cctcttttct
    13381 acagtattta aagatacccc aagaagctaa ttataacaag acgaactcca attcactgtt
    13441 ccttgcattc taaaacctta aataccagaa aacagctttt tcaaagttgt tttcaaagtt
    13501 ggcgtataac atagtatcga cggagccgat tttgaaaccg cggtgatcac aggcagcaac
    13561 gctctgtcat cgttacaatc aacatgctac cctccgcgag atcatccgtg tttcaaaccc
    13621 ggcagcttag ttgccgttct tccgaatagc atcggtaaca tgagcaaagt ctgccgcctt
    13681 acaacggctc tcccgctgac gccgtcccgg actgatgggc tgcctgtatc gagtggtgat
    13741 tttgtgccga gctgccggtc ggggagctgt tggctggctg gtggcaggat atattgtggt
    13801 gtaaacaaat tgacgcttag acaacttaat aacacattgc ggacgttttt aatgtactga
    13861 attaacgccg aattaattcg ggggatctgg attttagtac tggattttgg ttttaggaat
    13921 tagaaatttt attgatagaa gtattttaca aatacaaata catactaagg gtttcttata
    13981 tgctcaacac atgagcgaaa ccctatagga accctaattc ccttatctgg gaactactca
    14041 cacattatta tggagaaaat agagcttgtc gatcgttaat taatcacctc caagatcccg
    14101 gacgagtgct ggggcgtcgg tttccactat cggcgagtac ttctacacag ccatcggtcc
    14161 agacggccgc gcttctgcgg gcgatttgtg tacgcccgac agtcccggct ccggatcgga
    14221 cgattgcgtc gcatcgaccc tgcgcccaag ctgcatcatc gaaattgccg tcaaccaagc
    14281 tctgatagag ttggtcaaga ccaatgcgga gcatatacgc ccggagccgc ggcgatcctg
    14341 caagctccgg atgcctccgc tcgaagtagc gcgtctgctg ctccatacaa gccaaccacg
    14401 gcctccagaa gaagatgttg gcgacctcgt attgggaatc cccgaacatc gcctcgctcc
    14461 agtcaatgac cgctgttatg cggccattgt ccgtcaggac attgttggag ccgaaatccg
    14521 cgtgcacgag gtgccggact tcggggcagt cctcggccca aagcatcagc tcatcgagag
    14581 cctgcgcgac ggacgcactg acggtgtcgt ccatcacagt ttgccagtga tacacatggg
    14641 gatcagcaat cgcgcatatg aaatcacgcc atgtagtgta ttgaccgatt ccttgcggtc
    14701 cgaatgggcc gaacccgctc gtctggctaa gatcggccgc agcgatcgca tccatggcct
    14761 ccgcgaccgg ctgcagaaca gcgggcagtt cggtttcagg caggtcttgc aacgtgacac
    14821 cctgtgcacg gcgggagatg caataggtca ggctctcgct gaattcccca atgtcaagca
    14881 cttccggaat cgggagcgcg gccgatgcaa agtgccgata aacataacga tctttgtaga
    14941 aaccatcggc gcagctattt acccgcagga catatccacg ccctcctaca tcgaagctga
    15001 aagcacgaga ttcttcgccc tccgagagct gcatcaggtc ggacacgctg tcgaactttt
    15061 cgatcagaaa cttctcgaca gacgtcgcgg tgagttcagg ctttttcatc ctaggagaga
    15121 tagatttgta gagagagact ggtgatttca gcgtgtcctc tccaaatgaa atgaacttcc
    15181 ttatatagag gaaggtcttg cgaaggatag tgggattgtg cgtcatccct tacgtcagtg
    15241 gagatatcac atcaatccac ttgctttgaa gacgtggttg gaacgtcttc tttttccacg
    15301 atgctcctcg tgggtggggg tccatctttg ggaccactgt cggcagaggc atcttgaacg
    15361 atagcctttc ctttatcgca atgatggcat ttgtaggtgc caccttcctt ttctactgtc
    15421 cttttgatga agtgacagat agctgggcaa tggaatccga ggaggtttcc cgatattacc
    15481 ctttgttgaa aagtctcaat agccctttgg tcttctgaga ctgtatcttt gatattcttg
    15541 gagtagacga gagtgtcgtg ctccaccatg ttatcacatc aatccacttg ctttgaagac
    15601 gtggttggaa cgtcttcttt ttccacgatg ctcctcgtgg gtgggggtcc atctttggga
    15661 ccactgtcgg cagaggcatc ttgaacgata gcctttcctt tatcgcaatg atggcatttg
    15721 taggtgccac cttccttttc tactgtcctt ttgatgaagt gacagatagc tgggcaatgg
    15781 aatccgagga ggtttcccga tattaccctt tgttgaaaag tctcaatagc cctttggtct
    15841 tctgagactg tatctttgat attcttggag tagacgagag tgtcgtgctc caccatg
//
