LOCUS       sequence-26627-e         806 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 806)
  AUTHORS   Funakoshi M, Hochstrasser M
  TITLE     Small epitope-linker modules for PCR-based C-terminal tagging in 
            Saccharomyces cerevisiae.
  JOURNAL   Yeast. 2009 Feb 25. 26(3):185-192.
  PUBMED    19243080
REFERENCE   2  (bases 1 to 806)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast. 2009
            Feb 25. 26(3):185-192."
COMMENT     Sequence Label: pFA6a-6xGLY-HSV-HIS3MX6
FEATURES             Location/Qualifiers
     source          1..806
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(83..280)
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     CDS             complement(574..588)
                     /codon_start=1
                     /product="tag for protein cleavage using Ni2+ ion (Dang et 
                     al., 2019)"
                     /label=SNAC tag
                     /translation="GSHHW"
     gap             618..634
                     /estimated_length=17
ORIGIN
        1 cntaggcnnc tagtgganct gatatcatcg atgaattcga gctcgtttaa actggatggc
       61 ggcgttagta tcgaatcgac agcagtatag cgaccagcat tcacatacga ttgacgcatg
      121 atattacttt ctgcgcactt aacttcgcat ctgggcagat gatgtcgagg cgaaaaaaaa
      181 tataaatcac gctaacattt gattaaaata gaacaactac aatataaaaa aactatacaa
      241 atgacaagtt cttgaaaaca agaatctttt tattgtcagt acttcacatc aaaacacctt
      301 tggttgaggg aacgtcattg gtgccattgc tagaaatagc ttctcttatg gcaacagcca
      361 aagccttgaa cgcactctca cttctgtggt gatcgttgaa acctctcaga caatcaacat
      421 gcaaagtaat tctggccgcc tccgcgaaac tttccaaaaa gtgtggaatc atttcagtgg
      481 ataaatcacc aatcatctct ctcttcaatc caaggtcgat tacagcaaat ggtctactag
      541 ataaatcgac tacggcacgt gatagcgcct catccaatgg tgcgaaccca gtaccgaatc
      601 tttttacacc acgganannn nnnnnnnnnn nnnntttnna cnnnngccct aatgcgatac
      661 nnnnnncttc ggtagtatgg tgatcgtcaa tgtgcangtc accaatacat tcaacaataa
      721 gagaccaacc agagtgtttt gccaacgcat ggatcatatg atccaaaaag ccaacacctg
      781 tgtgaatatc gatganctgt gactga
//
