LOCUS       sequence-266272-       14841 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  iSTU-CRISPR/Cas12a DR-DR structure crRNA insertion vector with 
            OsCDPK2 intron.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14841)
  AUTHORS   Zhong Z, Liu S, Liu X, Liu B, Tang X, Ren Q, Zhou J, Zheng X, Qi Y, 
            Zhang Y
  TITLE     Intron-Based Single Transcript Unit CRISPR Systems for Plant Genome 
            Editing.
  JOURNAL   Rice (N Y). 2020 Feb 3;13(1):8. doi: 10.1186/s12284-020-0369-8.
  PUBMED    32016614
REFERENCE   2  (bases 1 to 14841)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Rice (N 
            Y)."; date: "2020-02-3"; volume: "13(1):8. doi"; pages: " 
            10.1186/s12284-020-0369-8"
COMMENT     Sequence Label: pGEL088
FEATURES             Location/Qualifiers
     source          1..14841
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        37..2033
                     /label=Ubi promoter
                     /note="maize polyubiquitin gene promoter"
     CDS             2048..2068
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6120..6167
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     terminator      6178..6426
                     /label=HSP terminator
                     /note="efficient transcription terminator from the 
                     Arabidopsis thaliana heat shock protein 18.2 gene (Nagaya 
                     et al., 2010)"
     misc_feature    6447..6471
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             7772..8401
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             8835..9902
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQA
                     AADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKR
                     DRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGR
                     VFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEAL
                     ISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLA
                     RRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILV
                     MRYRNLIEGEASAGS"
     rep_origin      9968..10162
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(10398..10420)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    10506..10646
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     10561..10580
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(10661..10678)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10832..11420)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10912..10931)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(11507..12301)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    12726..12750
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    12828..13002
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     promoter        complement(14092..14769)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
ORIGIN
        1 ggactagtag tcgtcagtca gtactagccg gcgcgcctgc agtgcagcgt gacccggtcg
       61 tgcccctctc ttgagataat gagcattgca tgtctaagtt ataaaaaatt accacatatt
      121 ttttttgtca cacttgtttg aagtgcagtt tatctatctt tatacatata tttaaacttt
      181 actctacgaa taatataatc tatagtacta caataatatc agtgttttag agaatcatat
      241 aaatgaacag ttagacatgg tctaaaggac aattgagtat tttgacaaca ggactctaca
      301 gttttatctt tttagtgtgc atgtgttctc cttttttttt gcaaatagct tcacctatat
      361 aatacttcat ccattttatt agtacatcca tttagggttt agggttaatg gtttttatag
      421 actaattttt ttagtacatc tattttattc tattttagcc tctaaattaa gaaaactaaa
      481 actctatttt agttttttta tttaataatt tagatataaa atagaataaa ataaagtgac
      541 taaaaattaa acaaataccc tttaagaaat taaaaaaact aaggaaacat ttttcttgtt
      601 tcgagtagat aatgccagcc tgttaaacgc cgtcgacgag tctaacggac accaaccagc
      661 gaaccagcag cgtcgcgtcg ggccaagcga agcagacggc acggcatctc tgtcgctgcc
      721 tctggacccc tctcgagagt tccgctccac cgttggactt gctccgctgt cggcatccag
      781 aaattgcgtg gcggagcggc agacgtgagc cggcacggca ggcggcctcc tcctcctctc
      841 acggcaccgg cagctacggg ggattccttt cccaccgctc cttcgctttc ccttcctcgc
      901 ccgccgtaat aaatagacac cccctccaca ccctctttcc ccaacctcgt gttgttcgga
      961 gcgcacacac acacaaccag aactccccca aatccacccg tcggcacctc cgcttcaagg
     1021 tacgccgctc gtcctccccc cccccccctc tctaccttct caagatcggc gttccggtcc
     1081 atggttaggg cccggtagtt ctacttctgt tcatgtttgt gttagatccg tgtttgtgtt
     1141 agatccgtgc tactagcgtt cgtacacgga tgcgacctgt acgtcagaca cgttctgatt
     1201 gctaacttgc cagtgtttct ctttggggaa tcctgggatg gctctagccg ttccgcagac
     1261 gggatcgatt tcatgatttt ttttgtttcg ttgcataggg tttggtttgc ccttttcctt
     1321 tatttcaata tatgccgtgc acttgtttgt cgggtcatct tttcatgctt ttttttgtct
     1381 tggttgtgat gatgtggtct ggttgggcgg tcgttcaaga tcggagtaga attaattctg
     1441 tttcaaacta cctggtggat ttattaattt tggatctgta tgtgtgtgcc atacatattc
     1501 atagttacga attgaagatg atggatggaa atatcgatct aggataggta tacatgttga
     1561 tgcgggtttt actgatgcat atacagagat gctttttgtt cgcttggttg tgatgatgtg
     1621 gtgtggttgg gcggtcgttc attcgttcaa gatcggagta gaatactgtt tcaaactacc
     1681 tggtgtattt attaattttg gaactgtatg tgtgtgtcat acatcttcat agttacgagt
     1741 ttaagatgga tggaaatatc gatctaggat aggtatacat gttgatgtgg gttttactga
     1801 tgcatataca tgatggcata tgcagcatct attcatatgc tctaaccttg agtacctatc
     1861 tattataata aacaagtatg ttttataatt attttgatct tgatatactt ggatgatggc
     1921 atatgcagca gctatatgtg gattttttta gccctgcctt catacgctat ttatttgctt
     1981 ggtactgttt cttttgtcga tgctcaccct gttgtttggt gttacttctg cagcctgcag
     2041 gatggctcct aagaagaagc ggaaggttgg tattcacggg gtgcctgcgg cttcaaagct
     2101 cgagaaattc accaactgtt attcgttgag caaaacactg cggtttaaag cgattccagt
     2161 cggcaagact caagagaata tagacaataa gcggctgttg gtggaagatg aaaagcgcgc
     2221 ggaagactac aaaggggtga agaagttgtt ggacagatac tacctctctt ttatcaatga
     2281 tgtcttgcac tcaatcaaat tgaagaatct gaacaactac atctccctct tcagaaagaa
     2341 aacaaggaca gaaaaggaga ataaggaact tgaaaatttg gagatcaatc tgaggaaaga
     2401 gatcgcgaaa gcctttaaag gcaacgaagg atacaaaagt ctgttcaaga aggatataat
     2461 tgagacaatt ttgccagagt tcctcgatga caaggacgag attgcgctgg tcaattcgtt
     2521 caacggattc acaacagcat tcacaggctt ctttgataat cgggaaaata tgttctctga
     2581 ggaggcaaag tccacttcta ttgcgttcag gtgtatcaat gagaatctca ctaggtacat
     2641 ttccaacatg gatatctttg agaaggttga cgcaattttt gacaagcacg aagttcagga
     2701 gattaaggag aagatcctca attccgatta tgacgttgag gacttcttcg aaggtgagtt
     2761 ttttaatttc gtgctcactc aagagggtat cgacgtgtat aatgcgatca tcggtgggtt
     2821 cgtgactgag tccggtgaaa agattaaggg attgaacgag tatatcaacc tttacaacca
     2881 aaagacgaaa cagaagctgc caaagttcaa gcctctttac aaacaggttc tttcagaccg
     2941 cgagtcactc tcgttctatg gggagggcta cacttcggat gaggaagtcc tggaggtgtt
     3001 caggaatact ctcaataaga attcggagat tttctcttct ataaaaaaac tggaaaagtt
     3061 gtttaagaat tttgacgaat actctagcgc cggcatattt gtgaaaaacg gcccggccat
     3121 atcaacgata agtaaagata tcttcggcga atggaacgtg atcagagaca aatggaacgc
     3181 ggagtatgac gatattcacc tgaagaagaa ggctgtcgta acggagaagt acgaggatga
     3241 tcgcaggaaa agcttcaaaa agatcggaag tttcagcctg gaacagttgc aggagtatgc
     3301 tgacgccgat cttagcgtcg tcgagaagtt gaaggagata atcatccaaa aggtcgatga
     3361 gatatataaa gtctatggat caagtgaaaa actgttcgac gccgacttcg ttttggagaa
     3421 gtccctgaag aagaacgacg ctgttgttgc cattatgaag gatctgctcg acagcgtgaa
     3481 gagtttcgag aactatatta aggctttttt cggggagggg aaggagacta acagagatga
     3541 gtccttctac ggagacttcg tcctcgcgta cgatatactc cttaaggtag accacatcta
     3601 cgacgcaatc agaaattacg tgacacaaaa gccgtacagc aaggacaagt tcaaactcta
     3661 cttccagaac ccccagttca tgggcggctg ggacaaggac aaggaaacgg attacagggc
     3721 tacgatcctg aggtatggtt caaaatacta cttggcgatt atggacaaga agtacgccaa
     3781 gtgtctccag aagattgaca aagacgatgt caatggcaat tatgagaaga tcaactacaa
     3841 gctgcttccg ggtccgaaca agatgctccc aaaggttttc ttcagcaaga aatggatggc
     3901 ctactataac ccaagcgagg acatccagaa gatttataag aacggtacgt tcaagaaggg
     3961 cgacatgttc aatcttaacg actgtcacaa gctgatcgac ttcttcaaag actcaattag
     4021 ccggtaccca aagtggtcta acgcctatga cttcaacttt tcggaaaccg agaagtacaa
     4081 ggatatagcc ggattttata gagaggtgga agagcagggc tacaaggtgt cattcgagtc
     4141 cgccagcaag aaggaagtgg acaagctcgt ggaagagggt aagctctaca tgttccagat
     4201 ttataataaa gactttagcg ataagagcca cgggacacct aatctccaca caatgtattt
     4261 caagctgctc ttcgacgaga ataaccacgg ccaaatcagg ttgtcaggag gggctgaact
     4321 cttcatgcgg cgcgcatccc tgaagaagga ggagcttgta gtccaccctg cgaatagtcc
     4381 aattgcgaat aagaacccgg acaatcctaa aaagactaca acattgagct acgacgtgta
     4441 caaggataag aggttttccg aggatcagta cgagctccac atcccgattg cgatcaacaa
     4501 gtgcccaaag aatattttca agataaacac agaggtgcgt gtactcctga agcatgacga
     4561 caatccttac gtcattggga ttgatcgggg cgagaggaac ctcctctata ttgtggtggt
     4621 ggacgggaag gggaacatag tcgaacagta ctcccttaac gaaataatta acaatttcaa
     4681 cggcatccgt atcaagaccg actaccattc gttgctggac aagaaggaga aggagagatt
     4741 tgaggcgcgg caaaattgga caagtatcga gaacatcaag gaactcaaag caggttatat
     4801 ctctcaagtt gtgcataaga tatgcgagct ggttgagaag tatgacgcag tgatcgctct
     4861 tgaggacctc aactcgggct ttaagaatag ccgcgttaaa gtggagaagc aggtctatca
     4921 aaagttcgag aagatgctta tagataagct caactacatg gtcgataaga aatcgaaccc
     4981 atgtgccacc ggcggcgcac tcaaaggtta ccaaataaca aacaaattcg agtccttcaa
     5041 atcgatgagt actcagaatg ggttcatatt ttatataccg gcgtggctta cgtctaagat
     5101 cgacccgtca actggttttg tcaacctgtt gaagacgaaa tacacgtcca ttgccgattc
     5161 gaaaaagttc atatctagtt ttgatcgtat tatgtacgtc ccagaggaag atcttttcga
     5221 gtttgctctc gactacaaaa acttttcgcg gaccgatgcg gattacatta aaaaatggaa
     5281 actctattcg tacggcaaca gaatcaggat ttttcgcaac cctaagaaga ataacgtctt
     5341 tgattgggag gaagtttgct tgactagcgc gtacaaggtc aatatgctgt caaaggcaaa
     5401 tttctactaa gtgtagatag agaccggggt ctccaatttc tactaagtgt agattttctt
     5461 ttcttttcct tttcgttgct tgttttcccc ttctgatgtt gtgtgttgag tttattgttt
     5521 ggtttgctgg tagatccagt atttggatca tttttccttt tttttttctt tcattgcttc
     5581 tgcggaggga tgagtttgtg catgaatctt ttgttggttg gattcgctta gttttcacac
     5641 tagtcctttg attcagcgca aatctttgtt aattggctcg cttaattctg acataaagtg
     5701 ttccttcttg gttctgcttg caggagctct ttaataagta tggcattaac taccaacagg
     5761 gtgatatcag agcactgctt tgcgaacaat ctgacaaggc tttctactca tccttcatgg
     5821 ctttgatgag cctgatgctc cagatgagaa attcaattac aggcagaacc gacgtggatt
     5881 tcttgatctc cccggttaaa aattctgatg gcatctttta cgatagcagg aactatgaag
     5941 cgcaagagaa tgcgattctg ccaaaaaatg cagacgccaa cggtgcctat aacatcgcca
     6001 ggaaagtcct gtgggcgatc ggccagttca aaaaggccga agacgaaaaa ttggacaagg
     6061 tcaaaatcgc tatcagcaac aaagagtggc tggagtatgc tcagacatcc gtaaagcata
     6121 agcgtcctgc tgccaccaaa aaggccggac aggctaagaa aaagaagtga tctagaatat
     6181 gaagatgaag atgaaatatt tggtgtgtca aataaaaagc ttgtgtgctt aagtttgtgt
     6241 ttttttcttg gcttgttgtg ttatgaattt gtggcttttt ctaatattaa atgaatgtaa
     6301 gatcacatta taatgaataa acaaatgttt ctataatcca ttgtgaatgt tttgttggat
     6361 ctcttctgca gcatataact actgtatgtg ctatggtatg gactatggaa tatgattaaa
     6421 gataaggagc tcaaactatc agtgtttgac aggatatatt ggcgggtaaa cctaagagaa
     6481 aagagcgttt attagaataa tcggatattt aaaagggcgt gaaaaggttt atccgttcgt
     6541 ccatttgtat gtgcatgcca accacagggt tcccctcggg atcaaagtac tttgatccaa
     6601 cccctccgct gctatagtgc agtcggcttc tgacgttcag tgcagccgtc ttctgaaaac
     6661 gacatgtcgc acaagtccta agttacgcga caggctgccg ccctgccctt ttcctggcgt
     6721 tttcttgtcg cgtgttttag tcgcataaag tagaatactt gcgactagaa ccggagacat
     6781 tacgccatga acaagagcgc cgccgctggc ctgctgggct atgcccgcgt cagcaccgac
     6841 gaccaggact tgaccaacca acgggccgaa ctgcacgcgg ccggctgcac caagctgttt
     6901 tccgagaaga tcaccggcac caggcgcgac cgcccggagc tggccaggat gcttgaccac
     6961 ctacgccctg gcgacgttgt gacagtgacc aggctagacc gcctggcccg cagcacccgc
     7021 gacctactgg acattgccga gcgcatccag gaggccggcg cgggcctgcg tagcctggca
     7081 gagccgtggg ccgacaccac cacgccggcc ggccgcatgg tgttgaccgt gttcgccggc
     7141 attgccgagt tcgagcgttc cctaatcatc gaccgcaccc ggagcgggcg cgaggccgcc
     7201 aaggcccgag gcgtgaagtt tggcccccgc cctaccctca ccccggcaca gatcgcgcac
     7261 gcccgcgagc tgatcgacca ggaaggccgc accgtgaaag aggcggctgc actgcttggc
     7321 gtgcatcgct cgaccctgta ccgcgcactt gagcgcagcg aggaagtgac gcccaccgag
     7381 gccaggcggc gcggtgcctt ccgtgaggac gcattgaccg aggccgacgc cctggcggcc
     7441 gccgagaatg aacgccaaga ggaacaagca tgaaaccgca ccaggacggc caggacgaac
     7501 cgtttttcat taccgaagag atcgaggcgg agatgatcgc ggccgggtac gtgttcgagc
     7561 cgcccgcgca cggctcaacc gtgcggctgc atgaaatcct ggccggtttg tctgatgcca
     7621 agctggcggc ctggccggcc agcttggccg ctgaagaaac cgagcgccgc cgtctaaaaa
     7681 ggtgatgtgt atttgagtaa aacagcttgc gtcatgcggt cgctgcgtat atgatgcgat
     7741 gagtaaataa acaaatacgc aaggggaacg catgaaggtt atcgctgtac ttaaccagaa
     7801 aggcgggtca ggcaagacga ccatcgcaac ccatctagcc cgcgccctgc aactcgccgg
     7861 ggccgatgtt ctgttagtcg attccgatcc ccagggcagt gcccgcgatt gggcggccgt
     7921 gcgggaagat caaccgctaa ccgttgtcgg catcgaccgc ccgacgattg accgcgacgt
     7981 gaaggccatc ggccggcgcg acttcgtagt gatcgacgga gcgccccagg cggcggactt
     8041 ggctgtgtcc gcgatcaagg cagccgactt cgtgctgatt ccggtgcagc caagccctta
     8101 cgacatatgg gccaccgccg acctggtgga gctggttaag cagcgcattg aggtcacgga
     8161 tggaaggcta caagcggcct ttgtcgtgtc gcgggcgatc aaaggcacgc gcatcggcgg
     8221 tgaggttgcc gaggcgctgg ccgggtacga gctgcccatt cttgagtccc gtatcacgca
     8281 gcgcgtgagc tacccaggca ctgccgccgc cggcacaacc gttcttgaat cagaacccga
     8341 gggcgacgct gcccgcgagg tccaggcgct ggccgctgaa attaaatcaa aactcatttg
     8401 agttaatgag gtaaagagaa aatgagcaaa agcacaaaca cgctaagtgc cggccgtccg
     8461 agcgcacgca gcagcaaggc tgcaacgttg gccagcctgg cagacacgcc agccatgaag
     8521 cgggtcaact ttcagttgcc ggcggaggat cacaccaagc tgaagatgta cgcggtacgc
     8581 caaggcaaga ccattaccga gctgctatct gaatacatcg cgcagctacc agagtaaatg
     8641 agcaaatgaa taaatgagta gatgaatttt agcggctaaa ggaggcggca tggaaaatca
     8701 agaacaacca ggcaccgacg ccgtggaatg ccccatgtgt ggaggaacgg gcggttggcc
     8761 aggcgtaagc ggctgggttg tctgccggcc ctgcaatggc actggaaccc ccaagcccga
     8821 ggaatcggcg tgacggtcgc aaaccatccg gcccggtaca aatcggcgcg gcgctgggtg
     8881 atgacctggt ggagaagttg aaggccgcgc aggccgccca gcggcaacgc atcgaggcag
     8941 aagcacgccc cggtgaatcg tggcaagcgg ccgctgatcg aatccgcaaa gaatcccggc
     9001 aaccgccggc agccggtgcg ccgtcgatta ggaagccgcc caagggcgac gagcaaccag
     9061 attttttcgt tccgatgctc tatgacgtgg gcacccgcga tagtcgcagc atcatggacg
     9121 tggccgtttt ccgtctgtcg aagcgtgacc gacgagctgg cgaggtgatc cgctacgagc
     9181 ttccagacgg gcacgtagag gtttccgcag ggccggccgg catggccagt gtgtgggatt
     9241 acgacctggt actgatggcg gtttcccatc taaccgaatc catgaaccga taccgggaag
     9301 ggaagggaga caagcccggc cgcgtgttcc gtccacacgt tgcggacgta ctcaagttct
     9361 gccggcgagc cgatggcgga aagcagaaag acgacctggt agaaacctgc attcggttaa
     9421 acaccacgca cgttgccatg cagcgtacga agaaggccaa gaacggccgc ctggtgacgg
     9481 tatccgaggg tgaagccttg attagccgct acaagatcgt aaagagcgaa accgggcggc
     9541 cggagtacat cgagatcgag ctagctgatt ggatgtaccg cgagatcaca gaaggcaaga
     9601 acccggacgt gctgacggtt caccccgatt actttttgat cgatcccggc atcggccgtt
     9661 ttctctaccg cctggcacgc cgcgccgcag gcaaggcaga agccagatgg ttgttcaaga
     9721 cgatctacga acgcagtggc agcgccggag agttcaagaa gttctgtttc accgtgcgca
     9781 agctgatcgg gtcaaatgac ctgccggagt acgatttgaa ggaggaggcg gggcaggctg
     9841 gcccgatcct agtcatgcgc taccgcaacc tgatcgaggg cgaagcatcc gccggttcct
     9901 aatgtacgga gcagatgcta gggcaaattg ccctagcagg ggaaaaaggt cgaaaaggcc
     9961 tctttcctgt ggatagcacg tacattggga acccaaagcc gtacattggg aaccggaacc
    10021 cgtacattgg gaacccaaag ccgtacattg ggaaccggtc acacatgtaa gtgactgata
    10081 taaaagagaa aaaaggcgat ttttccgcct aaaactcttt aaaacttatt aaaactctta
    10141 aaacccgcct ggcctgtgca taactgtctg gccagcgcac agccgaagag ctgcaaaaag
    10201 cgcctaccct tcggtcgctg cgctccctac gccccgccgc ttcgcgtcgg cctatcgcgg
    10261 ccgctggccg ctcaaaaatg gctggcctac ggccaggcaa tctaccaggg cgcggacaag
    10321 ccgcgccgtc gccactcgac cgccggcgcc cacatcaagg caccctgcct cgcgcgtttc
    10381 ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg aaacggtcac agcttgtctg
    10441 taagcggatg ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt
    10501 cggggcgcag ccatgaccca gtcacgtagc gatagcggag tgtatactgg cttaactatg
    10561 cggcatcaga gcagattgta ctgagagtgc accatatgcg gtgtgaaata ccgcacagat
    10621 gcgtaaggag aaaataccgc atcaggcgct cttccgcttc ctcgctcact gactcgctgc
    10681 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat
    10741 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
    10801 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
    10861 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
    10921 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
    10981 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
    11041 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
    11101 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
    11161 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
    11221 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat
    11281 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
    11341 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
    11401 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
    11461 ggaacgaaaa ctcacgttaa gggattttgg tcatgcattc taggtactaa aacaattcat
    11521 ccagtaaaat ataatatttt attttctccc aatcaggctt gatccccagt aagtcaaaaa
    11581 atagctcgac atactgttct tccccgatat cctccctgat cgaccggacg cagaaggcaa
    11641 tgtcatacca cttgtccgcc ctgccgcttc tcccaagatc aataaagcca cttactttgc
    11701 catctttcac aaagatgttg ctgtctccca ggtcgccgtg ggaaaagaca agttcctctt
    11761 cgggcttttc cgtctttaaa aaatcataca gctcgcgcgg atctttaaat ggagtgtctt
    11821 cttcccagtt ttcgcaatcc acatcggcca gatcgttatt cagtaagtaa tccaattcgg
    11881 ctaagcggct gtctaagcta ttcgtatagg gacaatccga tatgtcgatg gagtgaaaga
    11941 gcctgatgca ctccgcatac agctcgataa tcttttcagg gctttgttca tcttcatact
    12001 cttccgagca aaggacgcca tcggcctcac tcatgagcag attgctccag ccatcatgcc
    12061 gttcaaagtg caggaccttt ggaacaggca gctttccttc cagccatagc atcatgtcct
    12121 tttcccgttc cacatcatag gtggtccctt tataccggct gtccgtcatt tttaaatata
    12181 ggttttcatt ttctcccacc agcttatata ccttagcagg agacattcct tccgtatctt
    12241 ttacgcagcg gtatttttcg atcagttttt tcaattccgg tgatattctc attttagcca
    12301 tttattattt ccttcctctt ttctacagta tttaaagata ccccaagaag ctaattataa
    12361 caagacgaac tccaattcac tgttccttgc attctaaaac cttaaatacc agaaaacagc
    12421 tttttcaaag ttgttttcaa agttggcgta taacatagta tcgacggagc cgattttgaa
    12481 accgcggtga tcacaggcag caacgctctg tcatcgttac aatcaacatg ctaccctccg
    12541 cgagatcatc cgtgtttcaa acccggcagc ttagttgccg ttcttccgaa tagcatcggt
    12601 aacatgagca aagtctgccg ccttacaacg gctctcccgc tgacgccgtc ccggactgat
    12661 gggctgcctg tatcgagtgg tgattttgtg ccgagctgcc ggtcggggag ctgttggctg
    12721 gctggtggca ggatatattg tggtgtaaac aaattgacgc ttagacaact taataacaca
    12781 ttgcggacgt ttttaatgta ctgaattaac gccgaattaa ttcgggggat ctggatttta
    12841 gtactggatt ttggttttag gaattagaaa ttttattgat agaagtattt tacaaataca
    12901 aatacatact aagggtttct tatatgctca acacatgagc gaaaccctat aggaacccta
    12961 attcccttat ctgggaacta ctcacacatt attatggaga aaatagagct tgtcgatcgt
    13021 taattaatca cctccaagat cccggacgag tgctggggcg tcggtttcca ctatcggcga
    13081 gtacttctac acagccatcg gtccagacgg ccgcgcttct gcgggcgatt tgtgtacgcc
    13141 cgacagtccc ggctccggat cggacgattg cgtcgcatcg accctgcgcc caagctgcat
    13201 catcgaaatt gccgtcaacc aagctctgat agagttggtc aagaccaatg cggagcatat
    13261 acgcccggag ccgcggcgat cctgcaagct ccggatgcct ccgctcgaag tagcgcgtct
    13321 gctgctccat acaagccaac cacggcctcc agaagaagat gttggcgacc tcgtattggg
    13381 aatccccgaa catcgcctcg ctccagtcaa tgaccgctgt tatgcggcca ttgtccgtca
    13441 ggacattgtt ggagccgaaa tccgcgtgca cgaggtgccg gacttcgggg cagtcctcgg
    13501 cccaaagcat cagctcatcg agagcctgcg cgacggacgc actgacggtg tcgtccatca
    13561 cagtttgcca gtgatacaca tggggatcag caatcgcgca tatgaaatca cgccatgtag
    13621 tgtattgacc gattccttgc ggtccgaatg ggccgaaccc gctcgtctgg ctaagatcgg
    13681 ccgcagcgat cgcatccatg gcctccgcga ccggctgcag aacagcgggc agttcggttt
    13741 caggcaggtc ttgcaacgtg acaccctgtg cacggcggga gatgcaatag gtcaggctct
    13801 cgctgaattc cccaatgtca agcacttccg gaatcgggag cgcggccgat gcaaagtgcc
    13861 gataaacata acgatctttg tagaaaccat cggcgcagct atttacccgc aggacatatc
    13921 cacgccctcc tacatcgaag ctgaaagcac gagattcttc gccctccgag agctgcatca
    13981 ggtcggacac gctgtcgaac ttttcgatca gaaacttctc gacagacgtc gcggtgagtt
    14041 caggcttttt catcctagga gagatagatt tgtagagaga gactggtgat ttcagcgtgt
    14101 cctctccaaa tgaaatgaac ttccttatat agaggaaggt cttgcgaagg atagtgggat
    14161 tgtgcgtcat cccttacgtc agtggagata tcacatcaat ccacttgctt tgaagacgtg
    14221 gttggaacgt cttctttttc cacgatgctc ctcgtgggtg ggggtccatc tttgggacca
    14281 ctgtcggcag aggcatcttg aacgatagcc tttcctttat cgcaatgatg gcatttgtag
    14341 gtgccacctt ccttttctac tgtccttttg atgaagtgac agatagctgg gcaatggaat
    14401 ccgaggaggt ttcccgatat taccctttgt tgaaaagtct caatagccct ttggtcttct
    14461 gagactgtat ctttgatatt cttggagtag acgagagtgt cgtgctccac catgttatca
    14521 catcaatcca cttgctttga agacgtggtt ggaacgtctt ctttttccac gatgctcctc
    14581 gtgggtgggg gtccatcttt gggaccactg tcggcagagg catcttgaac gatagccttt
    14641 cctttatcgc aatgatggca tttgtaggtg ccaccttcct tttctactgt ccttttgatg
    14701 aagtgacaga tagctgggca atggaatccg aggaggtttc ccgatattac cctttgttga
    14761 aaagtctcaa tagccctttg gtcttctgag actgtatctt tgatattctt ggagtagacg
    14821 agagtgtcgt gctccaccat g
//
