LOCUS       sequence-266273-       14933 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  iSTU-CRISPR/Cas12a HH-HDV structure crRNA insertion vector with 
            OsCDPK2 intron.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14933)
  AUTHORS   Zhong Z, Liu S, Liu X, Liu B, Tang X, Ren Q, Zhou J, Zheng X, Qi Y, 
            Zhang Y
  TITLE     Intron-Based Single Transcript Unit CRISPR Systems for Plant Genome 
            Editing.
  JOURNAL   Rice (N Y). 2020 Feb 3;13(1):8. doi: 10.1186/s12284-020-0369-8.
  PUBMED    32016614
REFERENCE   2  (bases 1 to 14933)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Rice (N 
            Y)."; date: "2020-02-3"; volume: "13(1):8. doi"; pages: " 
            10.1186/s12284-020-0369-8"
COMMENT     Sequence Label: pGEL089
FEATURES             Location/Qualifiers
     source          1..14933
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        37..2033
                     /label=Ubi promoter
                     /note="maize polyubiquitin gene promoter"
     CDS             2048..2068
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     ncRNA           5405..5441
                     /product="self-cleaving hammerhead ribozyme (Ferre-D'Amare 
                     and Scott, 2010)"
                     /label=hammerhead ribozyme
                     /ncRNA_class="hammerhead_ribozyme"
     ncRNA           5479..5546
                     /product="hepatitis delta virus self-cleaving ribozyme 
                     (Ferre-D'Amare and Scott, 2010)"
                     /label=HDV ribozyme
                     /ncRNA_class="ribozyme"
     CDS             6212..6259
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     terminator      6270..6518
                     /label=HSP terminator
                     /note="efficient transcription terminator from the 
                     Arabidopsis thaliana heat shock protein 18.2 gene (Nagaya 
                     et al., 2010)"
     misc_feature    6539..6563
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             7864..8493
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             8927..9994
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQA
                     AADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKR
                     DRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGR
                     VFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEAL
                     ISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLA
                     RRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILV
                     MRYRNLIEGEASAGS"
     rep_origin      10060..10254
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(10490..10512)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    10598..10738
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     10653..10672
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(10753..10770)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10924..11512)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11004..11023)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(11599..12393)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    12818..12842
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    12920..13094
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     promoter        complement(14184..14861)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
ORIGIN
        1 ggactagtag tcgtcagtca gtactagccg gcgcgcctgc agtgcagcgt gacccggtcg
       61 tgcccctctc ttgagataat gagcattgca tgtctaagtt ataaaaaatt accacatatt
      121 ttttttgtca cacttgtttg aagtgcagtt tatctatctt tatacatata tttaaacttt
      181 actctacgaa taatataatc tatagtacta caataatatc agtgttttag agaatcatat
      241 aaatgaacag ttagacatgg tctaaaggac aattgagtat tttgacaaca ggactctaca
      301 gttttatctt tttagtgtgc atgtgttctc cttttttttt gcaaatagct tcacctatat
      361 aatacttcat ccattttatt agtacatcca tttagggttt agggttaatg gtttttatag
      421 actaattttt ttagtacatc tattttattc tattttagcc tctaaattaa gaaaactaaa
      481 actctatttt agttttttta tttaataatt tagatataaa atagaataaa ataaagtgac
      541 taaaaattaa acaaataccc tttaagaaat taaaaaaact aaggaaacat ttttcttgtt
      601 tcgagtagat aatgccagcc tgttaaacgc cgtcgacgag tctaacggac accaaccagc
      661 gaaccagcag cgtcgcgtcg ggccaagcga agcagacggc acggcatctc tgtcgctgcc
      721 tctggacccc tctcgagagt tccgctccac cgttggactt gctccgctgt cggcatccag
      781 aaattgcgtg gcggagcggc agacgtgagc cggcacggca ggcggcctcc tcctcctctc
      841 acggcaccgg cagctacggg ggattccttt cccaccgctc cttcgctttc ccttcctcgc
      901 ccgccgtaat aaatagacac cccctccaca ccctctttcc ccaacctcgt gttgttcgga
      961 gcgcacacac acacaaccag aactccccca aatccacccg tcggcacctc cgcttcaagg
     1021 tacgccgctc gtcctccccc cccccccctc tctaccttct caagatcggc gttccggtcc
     1081 atggttaggg cccggtagtt ctacttctgt tcatgtttgt gttagatccg tgtttgtgtt
     1141 agatccgtgc tactagcgtt cgtacacgga tgcgacctgt acgtcagaca cgttctgatt
     1201 gctaacttgc cagtgtttct ctttggggaa tcctgggatg gctctagccg ttccgcagac
     1261 gggatcgatt tcatgatttt ttttgtttcg ttgcataggg tttggtttgc ccttttcctt
     1321 tatttcaata tatgccgtgc acttgtttgt cgggtcatct tttcatgctt ttttttgtct
     1381 tggttgtgat gatgtggtct ggttgggcgg tcgttcaaga tcggagtaga attaattctg
     1441 tttcaaacta cctggtggat ttattaattt tggatctgta tgtgtgtgcc atacatattc
     1501 atagttacga attgaagatg atggatggaa atatcgatct aggataggta tacatgttga
     1561 tgcgggtttt actgatgcat atacagagat gctttttgtt cgcttggttg tgatgatgtg
     1621 gtgtggttgg gcggtcgttc attcgttcaa gatcggagta gaatactgtt tcaaactacc
     1681 tggtgtattt attaattttg gaactgtatg tgtgtgtcat acatcttcat agttacgagt
     1741 ttaagatgga tggaaatatc gatctaggat aggtatacat gttgatgtgg gttttactga
     1801 tgcatataca tgatggcata tgcagcatct attcatatgc tctaaccttg agtacctatc
     1861 tattataata aacaagtatg ttttataatt attttgatct tgatatactt ggatgatggc
     1921 atatgcagca gctatatgtg gattttttta gccctgcctt catacgctat ttatttgctt
     1981 ggtactgttt cttttgtcga tgctcaccct gttgtttggt gttacttctg cagcctgcag
     2041 gatggctcct aagaagaagc ggaaggttgg tattcacggg gtgcctgcgg cttcaaagct
     2101 cgagaaattc accaactgtt attcgttgag caaaacactg cggtttaaag cgattccagt
     2161 cggcaagact caagagaata tagacaataa gcggctgttg gtggaagatg aaaagcgcgc
     2221 ggaagactac aaaggggtga agaagttgtt ggacagatac tacctctctt ttatcaatga
     2281 tgtcttgcac tcaatcaaat tgaagaatct gaacaactac atctccctct tcagaaagaa
     2341 aacaaggaca gaaaaggaga ataaggaact tgaaaatttg gagatcaatc tgaggaaaga
     2401 gatcgcgaaa gcctttaaag gcaacgaagg atacaaaagt ctgttcaaga aggatataat
     2461 tgagacaatt ttgccagagt tcctcgatga caaggacgag attgcgctgg tcaattcgtt
     2521 caacggattc acaacagcat tcacaggctt ctttgataat cgggaaaata tgttctctga
     2581 ggaggcaaag tccacttcta ttgcgttcag gtgtatcaat gagaatctca ctaggtacat
     2641 ttccaacatg gatatctttg agaaggttga cgcaattttt gacaagcacg aagttcagga
     2701 gattaaggag aagatcctca attccgatta tgacgttgag gacttcttcg aaggtgagtt
     2761 ttttaatttc gtgctcactc aagagggtat cgacgtgtat aatgcgatca tcggtgggtt
     2821 cgtgactgag tccggtgaaa agattaaggg attgaacgag tatatcaacc tttacaacca
     2881 aaagacgaaa cagaagctgc caaagttcaa gcctctttac aaacaggttc tttcagaccg
     2941 cgagtcactc tcgttctatg gggagggcta cacttcggat gaggaagtcc tggaggtgtt
     3001 caggaatact ctcaataaga attcggagat tttctcttct ataaaaaaac tggaaaagtt
     3061 gtttaagaat tttgacgaat actctagcgc cggcatattt gtgaaaaacg gcccggccat
     3121 atcaacgata agtaaagata tcttcggcga atggaacgtg atcagagaca aatggaacgc
     3181 ggagtatgac gatattcacc tgaagaagaa ggctgtcgta acggagaagt acgaggatga
     3241 tcgcaggaaa agcttcaaaa agatcggaag tttcagcctg gaacagttgc aggagtatgc
     3301 tgacgccgat cttagcgtcg tcgagaagtt gaaggagata atcatccaaa aggtcgatga
     3361 gatatataaa gtctatggat caagtgaaaa actgttcgac gccgacttcg ttttggagaa
     3421 gtccctgaag aagaacgacg ctgttgttgc cattatgaag gatctgctcg acagcgtgaa
     3481 gagtttcgag aactatatta aggctttttt cggggagggg aaggagacta acagagatga
     3541 gtccttctac ggagacttcg tcctcgcgta cgatatactc cttaaggtag accacatcta
     3601 cgacgcaatc agaaattacg tgacacaaaa gccgtacagc aaggacaagt tcaaactcta
     3661 cttccagaac ccccagttca tgggcggctg ggacaaggac aaggaaacgg attacagggc
     3721 tacgatcctg aggtatggtt caaaatacta cttggcgatt atggacaaga agtacgccaa
     3781 gtgtctccag aagattgaca aagacgatgt caatggcaat tatgagaaga tcaactacaa
     3841 gctgcttccg ggtccgaaca agatgctccc aaaggttttc ttcagcaaga aatggatggc
     3901 ctactataac ccaagcgagg acatccagaa gatttataag aacggtacgt tcaagaaggg
     3961 cgacatgttc aatcttaacg actgtcacaa gctgatcgac ttcttcaaag actcaattag
     4021 ccggtaccca aagtggtcta acgcctatga cttcaacttt tcggaaaccg agaagtacaa
     4081 ggatatagcc ggattttata gagaggtgga agagcagggc tacaaggtgt cattcgagtc
     4141 cgccagcaag aaggaagtgg acaagctcgt ggaagagggt aagctctaca tgttccagat
     4201 ttataataaa gactttagcg ataagagcca cgggacacct aatctccaca caatgtattt
     4261 caagctgctc ttcgacgaga ataaccacgg ccaaatcagg ttgtcaggag gggctgaact
     4321 cttcatgcgg cgcgcatccc tgaagaagga ggagcttgta gtccaccctg cgaatagtcc
     4381 aattgcgaat aagaacccgg acaatcctaa aaagactaca acattgagct acgacgtgta
     4441 caaggataag aggttttccg aggatcagta cgagctccac atcccgattg cgatcaacaa
     4501 gtgcccaaag aatattttca agataaacac agaggtgcgt gtactcctga agcatgacga
     4561 caatccttac gtcattggga ttgatcgggg cgagaggaac ctcctctata ttgtggtggt
     4621 ggacgggaag gggaacatag tcgaacagta ctcccttaac gaaataatta acaatttcaa
     4681 cggcatccgt atcaagaccg actaccattc gttgctggac aagaaggaga aggagagatt
     4741 tgaggcgcgg caaaattgga caagtatcga gaacatcaag gaactcaaag caggttatat
     4801 ctctcaagtt gtgcataaga tatgcgagct ggttgagaag tatgacgcag tgatcgctct
     4861 tgaggacctc aactcgggct ttaagaatag ccgcgttaaa gtggagaagc aggtctatca
     4921 aaagttcgag aagatgctta tagataagct caactacatg gtcgataaga aatcgaaccc
     4981 atgtgccacc ggcggcgcac tcaaaggtta ccaaataaca aacaaattcg agtccttcaa
     5041 atcgatgagt actcagaatg ggttcatatt ttatataccg gcgtggctta cgtctaagat
     5101 cgacccgtca actggttttg tcaacctgtt gaagacgaaa tacacgtcca ttgccgattc
     5161 gaaaaagttc atatctagtt ttgatcgtat tatgtacgtc ccagaggaag atcttttcga
     5221 gtttgctctc gactacaaaa acttttcgcg gaccgatgcg gattacatta aaaaatggaa
     5281 actctattcg tacggcaaca gaatcaggat ttttcgcaac cctaagaaga ataacgtctt
     5341 tgattgggag gaagtttgct tgactagcgc gtacaaggtc aatatgctgt caaaggcaaa
     5401 attactgatg agtccgtgag gacgaaacga gtaagctcgt ctaatttcta ctaagtgtag
     5461 atagagaccg gggtctccgg ccggcatggt cccagcctcc tcgctggcgc cggctgggca
     5521 acatgcttcg gcatggcgaa tgggactttc ttttcttttc cttttcgttg cttgttttcc
     5581 ccttctgatg ttgtgtgttg agtttattgt ttggtttgct ggtagatcca gtatttggat
     5641 catttttcct tttttttttc tttcattgct tctgcggagg gatgagtttg tgcatgaatc
     5701 ttttgttggt tggattcgct tagttttcac actagtcctt tgattcagcg caaatctttg
     5761 ttaattggct cgcttaattc tgacataaag tgttccttct tggttctgct tgcaggagct
     5821 ctttaataag tatggcatta actaccaaca gggtgatatc agagcactgc tttgcgaaca
     5881 atctgacaag gctttctact catccttcat ggctttgatg agcctgatgc tccagatgag
     5941 aaattcaatt acaggcagaa ccgacgtgga tttcttgatc tccccggtta aaaattctga
     6001 tggcatcttt tacgatagca ggaactatga agcgcaagag aatgcgattc tgccaaaaaa
     6061 tgcagacgcc aacggtgcct ataacatcgc caggaaagtc ctgtgggcga tcggccagtt
     6121 caaaaaggcc gaagacgaaa aattggacaa ggtcaaaatc gctatcagca acaaagagtg
     6181 gctggagtat gctcagacat ccgtaaagca taagcgtcct gctgccacca aaaaggccgg
     6241 acaggctaag aaaaagaagt gatctagaat atgaagatga agatgaaata tttggtgtgt
     6301 caaataaaaa gcttgtgtgc ttaagtttgt gtttttttct tggcttgttg tgttatgaat
     6361 ttgtggcttt ttctaatatt aaatgaatgt aagatcacat tataatgaat aaacaaatgt
     6421 ttctataatc cattgtgaat gttttgttgg atctcttctg cagcatataa ctactgtatg
     6481 tgctatggta tggactatgg aatatgatta aagataagga gctcaaacta tcagtgtttg
     6541 acaggatata ttggcgggta aacctaagag aaaagagcgt ttattagaat aatcggatat
     6601 ttaaaagggc gtgaaaaggt ttatccgttc gtccatttgt atgtgcatgc caaccacagg
     6661 gttcccctcg ggatcaaagt actttgatcc aacccctccg ctgctatagt gcagtcggct
     6721 tctgacgttc agtgcagccg tcttctgaaa acgacatgtc gcacaagtcc taagttacgc
     6781 gacaggctgc cgccctgccc ttttcctggc gttttcttgt cgcgtgtttt agtcgcataa
     6841 agtagaatac ttgcgactag aaccggagac attacgccat gaacaagagc gccgccgctg
     6901 gcctgctggg ctatgcccgc gtcagcaccg acgaccagga cttgaccaac caacgggccg
     6961 aactgcacgc ggccggctgc accaagctgt tttccgagaa gatcaccggc accaggcgcg
     7021 accgcccgga gctggccagg atgcttgacc acctacgccc tggcgacgtt gtgacagtga
     7081 ccaggctaga ccgcctggcc cgcagcaccc gcgacctact ggacattgcc gagcgcatcc
     7141 aggaggccgg cgcgggcctg cgtagcctgg cagagccgtg ggccgacacc accacgccgg
     7201 ccggccgcat ggtgttgacc gtgttcgccg gcattgccga gttcgagcgt tccctaatca
     7261 tcgaccgcac ccggagcggg cgcgaggccg ccaaggcccg aggcgtgaag tttggccccc
     7321 gccctaccct caccccggca cagatcgcgc acgcccgcga gctgatcgac caggaaggcc
     7381 gcaccgtgaa agaggcggct gcactgcttg gcgtgcatcg ctcgaccctg taccgcgcac
     7441 ttgagcgcag cgaggaagtg acgcccaccg aggccaggcg gcgcggtgcc ttccgtgagg
     7501 acgcattgac cgaggccgac gccctggcgg ccgccgagaa tgaacgccaa gaggaacaag
     7561 catgaaaccg caccaggacg gccaggacga accgtttttc attaccgaag agatcgaggc
     7621 ggagatgatc gcggccgggt acgtgttcga gccgcccgcg cacggctcaa ccgtgcggct
     7681 gcatgaaatc ctggccggtt tgtctgatgc caagctggcg gcctggccgg ccagcttggc
     7741 cgctgaagaa accgagcgcc gccgtctaaa aaggtgatgt gtatttgagt aaaacagctt
     7801 gcgtcatgcg gtcgctgcgt atatgatgcg atgagtaaat aaacaaatac gcaaggggaa
     7861 cgcatgaagg ttatcgctgt acttaaccag aaaggcgggt caggcaagac gaccatcgca
     7921 acccatctag cccgcgccct gcaactcgcc ggggccgatg ttctgttagt cgattccgat
     7981 ccccagggca gtgcccgcga ttgggcggcc gtgcgggaag atcaaccgct aaccgttgtc
     8041 ggcatcgacc gcccgacgat tgaccgcgac gtgaaggcca tcggccggcg cgacttcgta
     8101 gtgatcgacg gagcgcccca ggcggcggac ttggctgtgt ccgcgatcaa ggcagccgac
     8161 ttcgtgctga ttccggtgca gccaagccct tacgacatat gggccaccgc cgacctggtg
     8221 gagctggtta agcagcgcat tgaggtcacg gatggaaggc tacaagcggc ctttgtcgtg
     8281 tcgcgggcga tcaaaggcac gcgcatcggc ggtgaggttg ccgaggcgct ggccgggtac
     8341 gagctgccca ttcttgagtc ccgtatcacg cagcgcgtga gctacccagg cactgccgcc
     8401 gccggcacaa ccgttcttga atcagaaccc gagggcgacg ctgcccgcga ggtccaggcg
     8461 ctggccgctg aaattaaatc aaaactcatt tgagttaatg aggtaaagag aaaatgagca
     8521 aaagcacaaa cacgctaagt gccggccgtc cgagcgcacg cagcagcaag gctgcaacgt
     8581 tggccagcct ggcagacacg ccagccatga agcgggtcaa ctttcagttg ccggcggagg
     8641 atcacaccaa gctgaagatg tacgcggtac gccaaggcaa gaccattacc gagctgctat
     8701 ctgaatacat cgcgcagcta ccagagtaaa tgagcaaatg aataaatgag tagatgaatt
     8761 ttagcggcta aaggaggcgg catggaaaat caagaacaac caggcaccga cgccgtggaa
     8821 tgccccatgt gtggaggaac gggcggttgg ccaggcgtaa gcggctgggt tgtctgccgg
     8881 ccctgcaatg gcactggaac ccccaagccc gaggaatcgg cgtgacggtc gcaaaccatc
     8941 cggcccggta caaatcggcg cggcgctggg tgatgacctg gtggagaagt tgaaggccgc
     9001 gcaggccgcc cagcggcaac gcatcgaggc agaagcacgc cccggtgaat cgtggcaagc
     9061 ggccgctgat cgaatccgca aagaatcccg gcaaccgccg gcagccggtg cgccgtcgat
     9121 taggaagccg cccaagggcg acgagcaacc agattttttc gttccgatgc tctatgacgt
     9181 gggcacccgc gatagtcgca gcatcatgga cgtggccgtt ttccgtctgt cgaagcgtga
     9241 ccgacgagct ggcgaggtga tccgctacga gcttccagac gggcacgtag aggtttccgc
     9301 agggccggcc ggcatggcca gtgtgtggga ttacgacctg gtactgatgg cggtttccca
     9361 tctaaccgaa tccatgaacc gataccggga agggaaggga gacaagcccg gccgcgtgtt
     9421 ccgtccacac gttgcggacg tactcaagtt ctgccggcga gccgatggcg gaaagcagaa
     9481 agacgacctg gtagaaacct gcattcggtt aaacaccacg cacgttgcca tgcagcgtac
     9541 gaagaaggcc aagaacggcc gcctggtgac ggtatccgag ggtgaagcct tgattagccg
     9601 ctacaagatc gtaaagagcg aaaccgggcg gccggagtac atcgagatcg agctagctga
     9661 ttggatgtac cgcgagatca cagaaggcaa gaacccggac gtgctgacgg ttcaccccga
     9721 ttactttttg atcgatcccg gcatcggccg ttttctctac cgcctggcac gccgcgccgc
     9781 aggcaaggca gaagccagat ggttgttcaa gacgatctac gaacgcagtg gcagcgccgg
     9841 agagttcaag aagttctgtt tcaccgtgcg caagctgatc gggtcaaatg acctgccgga
     9901 gtacgatttg aaggaggagg cggggcaggc tggcccgatc ctagtcatgc gctaccgcaa
     9961 cctgatcgag ggcgaagcat ccgccggttc ctaatgtacg gagcagatgc tagggcaaat
    10021 tgccctagca ggggaaaaag gtcgaaaagg cctctttcct gtggatagca cgtacattgg
    10081 gaacccaaag ccgtacattg ggaaccggaa cccgtacatt gggaacccaa agccgtacat
    10141 tgggaaccgg tcacacatgt aagtgactga tataaaagag aaaaaaggcg atttttccgc
    10201 ctaaaactct ttaaaactta ttaaaactct taaaacccgc ctggcctgtg cataactgtc
    10261 tggccagcgc acagccgaag agctgcaaaa agcgcctacc cttcggtcgc tgcgctccct
    10321 acgccccgcc gcttcgcgtc ggcctatcgc ggccgctggc cgctcaaaaa tggctggcct
    10381 acggccaggc aatctaccag ggcgcggaca agccgcgccg tcgccactcg accgccggcg
    10441 cccacatcaa ggcaccctgc ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca
    10501 tgcagctccc ggaaacggtc acagcttgtc tgtaagcgga tgccgggagc agacaagccc
    10561 gtcagggcgc gtcagcgggt gttggcgggt gtcggggcgc agccatgacc cagtcacgta
    10621 gcgatagcgg agtgtatact ggcttaacta tgcggcatca gagcagattg tactgagagt
    10681 gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc gcatcaggcg
    10741 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
    10801 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
    10861 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
    10921 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
    10981 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
    11041 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
    11101 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
    11161 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
    11221 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
    11281 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
    11341 gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt
    11401 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
    11461 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
    11521 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
    11581 ggtcatgcat tctaggtact aaaacaattc atccagtaaa atataatatt ttattttctc
    11641 ccaatcaggc ttgatcccca gtaagtcaaa aaatagctcg acatactgtt cttccccgat
    11701 atcctccctg atcgaccgga cgcagaaggc aatgtcatac cacttgtccg ccctgccgct
    11761 tctcccaaga tcaataaagc cacttacttt gccatctttc acaaagatgt tgctgtctcc
    11821 caggtcgccg tgggaaaaga caagttcctc ttcgggcttt tccgtcttta aaaaatcata
    11881 cagctcgcgc ggatctttaa atggagtgtc ttcttcccag ttttcgcaat ccacatcggc
    11941 cagatcgtta ttcagtaagt aatccaattc ggctaagcgg ctgtctaagc tattcgtata
    12001 gggacaatcc gatatgtcga tggagtgaaa gagcctgatg cactccgcat acagctcgat
    12061 aatcttttca gggctttgtt catcttcata ctcttccgag caaaggacgc catcggcctc
    12121 actcatgagc agattgctcc agccatcatg ccgttcaaag tgcaggacct ttggaacagg
    12181 cagctttcct tccagccata gcatcatgtc cttttcccgt tccacatcat aggtggtccc
    12241 tttataccgg ctgtccgtca tttttaaata taggttttca ttttctccca ccagcttata
    12301 taccttagca ggagacattc cttccgtatc ttttacgcag cggtattttt cgatcagttt
    12361 tttcaattcc ggtgatattc tcattttagc catttattat ttccttcctc ttttctacag
    12421 tatttaaaga taccccaaga agctaattat aacaagacga actccaattc actgttcctt
    12481 gcattctaaa accttaaata ccagaaaaca gctttttcaa agttgttttc aaagttggcg
    12541 tataacatag tatcgacgga gccgattttg aaaccgcggt gatcacaggc agcaacgctc
    12601 tgtcatcgtt acaatcaaca tgctaccctc cgcgagatca tccgtgtttc aaacccggca
    12661 gcttagttgc cgttcttccg aatagcatcg gtaacatgag caaagtctgc cgccttacaa
    12721 cggctctccc gctgacgccg tcccggactg atgggctgcc tgtatcgagt ggtgattttg
    12781 tgccgagctg ccggtcgggg agctgttggc tggctggtgg caggatatat tgtggtgtaa
    12841 acaaattgac gcttagacaa cttaataaca cattgcggac gtttttaatg tactgaatta
    12901 acgccgaatt aattcggggg atctggattt tagtactgga ttttggtttt aggaattaga
    12961 aattttattg atagaagtat tttacaaata caaatacata ctaagggttt cttatatgct
    13021 caacacatga gcgaaaccct ataggaaccc taattccctt atctgggaac tactcacaca
    13081 ttattatgga gaaaatagag cttgtcgatc gttaattaat cacctccaag atcccggacg
    13141 agtgctgggg cgtcggtttc cactatcggc gagtacttct acacagccat cggtccagac
    13201 ggccgcgctt ctgcgggcga tttgtgtacg cccgacagtc ccggctccgg atcggacgat
    13261 tgcgtcgcat cgaccctgcg cccaagctgc atcatcgaaa ttgccgtcaa ccaagctctg
    13321 atagagttgg tcaagaccaa tgcggagcat atacgcccgg agccgcggcg atcctgcaag
    13381 ctccggatgc ctccgctcga agtagcgcgt ctgctgctcc atacaagcca accacggcct
    13441 ccagaagaag atgttggcga cctcgtattg ggaatccccg aacatcgcct cgctccagtc
    13501 aatgaccgct gttatgcggc cattgtccgt caggacattg ttggagccga aatccgcgtg
    13561 cacgaggtgc cggacttcgg ggcagtcctc ggcccaaagc atcagctcat cgagagcctg
    13621 cgcgacggac gcactgacgg tgtcgtccat cacagtttgc cagtgataca catggggatc
    13681 agcaatcgcg catatgaaat cacgccatgt agtgtattga ccgattcctt gcggtccgaa
    13741 tgggccgaac ccgctcgtct ggctaagatc ggccgcagcg atcgcatcca tggcctccgc
    13801 gaccggctgc agaacagcgg gcagttcggt ttcaggcagg tcttgcaacg tgacaccctg
    13861 tgcacggcgg gagatgcaat aggtcaggct ctcgctgaat tccccaatgt caagcacttc
    13921 cggaatcggg agcgcggccg atgcaaagtg ccgataaaca taacgatctt tgtagaaacc
    13981 atcggcgcag ctatttaccc gcaggacata tccacgccct cctacatcga agctgaaagc
    14041 acgagattct tcgccctccg agagctgcat caggtcggac acgctgtcga acttttcgat
    14101 cagaaacttc tcgacagacg tcgcggtgag ttcaggcttt ttcatcctag gagagataga
    14161 tttgtagaga gagactggtg atttcagcgt gtcctctcca aatgaaatga acttccttat
    14221 atagaggaag gtcttgcgaa ggatagtggg attgtgcgtc atcccttacg tcagtggaga
    14281 tatcacatca atccacttgc tttgaagacg tggttggaac gtcttctttt tccacgatgc
    14341 tcctcgtggg tgggggtcca tctttgggac cactgtcggc agaggcatct tgaacgatag
    14401 cctttccttt atcgcaatga tggcatttgt aggtgccacc ttccttttct actgtccttt
    14461 tgatgaagtg acagatagct gggcaatgga atccgaggag gtttcccgat attacccttt
    14521 gttgaaaagt ctcaatagcc ctttggtctt ctgagactgt atctttgata ttcttggagt
    14581 agacgagagt gtcgtgctcc accatgttat cacatcaatc cacttgcttt gaagacgtgg
    14641 ttggaacgtc ttctttttcc acgatgctcc tcgtgggtgg gggtccatct ttgggaccac
    14701 tgtcggcaga ggcatcttga acgatagcct ttcctttatc gcaatgatgg catttgtagg
    14761 tgccaccttc cttttctact gtccttttga tgaagtgaca gatagctggg caatggaatc
    14821 cgaggaggtt tcccgatatt accctttgtt gaaaagtctc aatagccctt tggtcttctg
    14881 agactgtatc tttgatattc ttggagtaga cgagagtgtc gtgctccacc atg
//
