LOCUS       sequence-266277-       15191 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  STU2.0 LbCas12a for plant genome editing.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15191)
  AUTHORS   Tang X, Ren Q, Yang L, Bao Y, Zhong Z, He Y, Liu S, Qi C, Liu B, 
            Wang Y, Sretenovic S, Zhang Y, Zheng X, Zhang T, Qi Y, Zhang Y
  TITLE     Single transcript unit CRISPR 2.0 systems for robust Cas9 and Cas12a
            mediated plant genome editing.
  JOURNAL   Plant Biotechnol J. 2019 Jul;17(7):1431-1445. doi: 
            10.1111/pbi.13068. Epub 2019 Jan 17.
  PUBMED    30582653
REFERENCE   2  (bases 1 to 15191)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/pbi.13068";
            journalName: "Plant Biotechnol J"; date: "2019-07"; volume: "17"; 
            issue: "7"; pages: "1431-1445"
COMMENT     Sequence Label: pGEL032
FEATURES             Location/Qualifiers
     source          1..15191
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        8..2004
                     /label=Ubi promoter
                     /note="maize polyubiquitin gene promoter"
     CDS             2019..2039
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2064..5744
                     /codon_start=1
                     /product="CRISPR-associated protein from Lachnospiraceae 
                     bacterium ND2006 (Zetsche et al., 2015)"
                     /label=LbCpf1
                     /translation="SKLEKFTNCYSLSKTLRFKAIPVGKTQENIDNKRLLVEDEKRAED
                     YKGVKKLLDRYYLSFINDVLHSIKLKNLNNYISLFRKKTRTEKENKELENLEINLRKEI
                     AKAFKGNEGYKSLFKKDIIETILPEFLDDKDEIALVNSFNGFTTAFTGFFDNRENMFSE
                     EAKSTSIAFRCINENLTRYISNMDIFEKVDAIFDKHEVQEIKEKILNSDYDVEDFFEGE
                     FFNFVLTQEGIDVYNAIIGGFVTESGEKIKGLNEYINLYNQKTKQKLPKFKPLYKQVLS
                     DRESLSFYGEGYTSDEEVLEVFRNTLNKNSEIFSSIKKLEKLFKNFDEYSSAGIFVKNG
                     PAISTISKDIFGEWNVIRDKWNAEYDDIHLKKKAVVTEKYEDDRRKSFKKIGSFSLEQL
                     QEYADADLSVVEKLKEIIIQKVDEIYKVYGSSEKLFDADFVLEKSLKKNDAVVAIMKDL
                     LDSVKSFENYIKAFFGEGKETNRDESFYGDFVLAYDILLKVDHIYDAIRNYVTQKPYSK
                     DKFKLYFQNPQFMGGWDKDKETDYRATILRYGSKYYLAIMDKKYAKCLQKIDKDDVNGN
                     YEKINYKLLPGPNKMLPKVFFSKKWMAYYNPSEDIQKIYKNGTFKKGDMFNLNDCHKLI
                     DFFKDSISRYPKWSNAYDFNFSETEKYKDIAGFYREVEEQGYKVSFESASKKEVDKLVE
                     EGKLYMFQIYNKDFSDKSHGTPNLHTMYFKLLFDENNHGQIRLSGGAELFMRRASLKKE
                     ELVVHPANSPIANKNPDNPKKTTTLSYDVYKDKRFSEDQYELHIPIAINKCPKNIFKIN
                     TEVRVLLKHDDNPYVIGIDRGERNLLYIVVVDGKGNIVEQYSLNEIINNFNGIRIKTDY
                     HSLLDKKEKERFEARQNWTSIENIKELKAGYISQVVHKICELVEKYDAVIALEDLNSGF
                     KNSRVKVEKQVYQKFEKMLIDKLNYMVDKKSNPCATGGALKGYQITNKFESFKSMSTQN
                     GFIFYIPAWLTSKIDPSTGFVNLLKTKYTSIADSKKFISSFDRIMYVPEEDLFEFALDY
                     KNFSRTDADYIKKWKLYSYGNRIRIFRNPKKNNVFDWEEVCLTSAYKELFNKYGINYQQ
                     GDIRALLCEQSDKAFYSSFMALMSLMLQMRNSITGRTDVDFLISPVKNSDGIFYDSRNY
                     EAQENAILPKNADANGAYNIARKVLWAIGQFKKAEDEKLDKVKIAISNKEWLEYAQTSV
                     KH"
     CDS             5745..5792
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             complement(5853..6158)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(5955..5974)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     terminator      6528..6776
                     /label=HSP terminator
                     /note="efficient transcription terminator from the 
                     Arabidopsis thaliana heat shock protein 18.2 gene (Nagaya 
                     et al., 2010)"
     misc_feature    6797..6821
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             8122..8751
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             9185..10252
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQA
                     AADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKR
                     DRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGR
                     VFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEAL
                     ISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLA
                     RRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILV
                     MRYRNLIEGEASAGS"
     rep_origin      10318..10512
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(10748..10770)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    10856..10996
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     10911..10930
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(11011..11028)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(11182..11770)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11262..11281)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(11857..12651)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    13076..13100
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    13178..13352
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     promoter        complement(14442..15119)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
ORIGIN
        1 ggcgcgcctg cagtgcagcg tgacccggtc gtgcccctct cttgagataa tgagcattgc
       61 atgtctaagt tataaaaaat taccacatat tttttttgtc acacttgttt gaagtgcagt
      121 ttatctatct ttatacatat atttaaactt tactctacga ataatataat ctatagtact
      181 acaataatat cagtgtttta gagaatcata taaatgaaca gttagacatg gtctaaagga
      241 caattgagta ttttgacaac aggactctac agttttatct ttttagtgtg catgtgttct
      301 cctttttttt tgcaaatagc ttcacctata taatacttca tccattttat tagtacatcc
      361 atttagggtt tagggttaat ggtttttata gactaatttt tttagtacat ctattttatt
      421 ctattttagc ctctaaatta agaaaactaa aactctattt tagttttttt atttaataat
      481 ttagatataa aatagaataa aataaagtga ctaaaaatta aacaaatacc ctttaagaaa
      541 ttaaaaaaac taaggaaaca tttttcttgt ttcgagtaga taatgccagc ctgttaaacg
      601 ccgtcgacga gtctaacgga caccaaccag cgaaccagca gcgtcgcgtc gggccaagcg
      661 aagcagacgg cacggcatct ctgtcgctgc ctctggaccc ctctcgagag ttccgctcca
      721 ccgttggact tgctccgctg tcggcatcca gaaattgcgt ggcggagcgg cagacgtgag
      781 ccggcacggc aggcggcctc ctcctcctct cacggcaccg gcagctacgg gggattcctt
      841 tcccaccgct ccttcgcttt cccttcctcg cccgccgtaa taaatagaca ccccctccac
      901 accctctttc cccaacctcg tgttgttcgg agcgcacaca cacacaacca gaactccccc
      961 aaatccaccc gtcggcacct ccgcttcaag gtacgccgct cgtcctcccc ccccccccct
     1021 ctctaccttc tcaagatcgg cgttccggtc catggttagg gcccggtagt tctacttctg
     1081 ttcatgtttg tgttagatcc gtgtttgtgt tagatccgtg ctactagcgt tcgtacacgg
     1141 atgcgacctg tacgtcagac acgttctgat tgctaacttg ccagtgtttc tctttgggga
     1201 atcctgggat ggctctagcc gttccgcaga cgggatcgat ttcatgattt tttttgtttc
     1261 gttgcatagg gtttggtttg cccttttcct ttatttcaat atatgccgtg cacttgtttg
     1321 tcgggtcatc ttttcatgct tttttttgtc ttggttgtga tgatgtggtc tggttgggcg
     1381 gtcgttcaag atcggagtag aattaattct gtttcaaact acctggtgga tttattaatt
     1441 ttggatctgt atgtgtgtgc catacatatt catagttacg aattgaagat gatggatgga
     1501 aatatcgatc taggataggt atacatgttg atgcgggttt tactgatgca tatacagaga
     1561 tgctttttgt tcgcttggtt gtgatgatgt ggtgtggttg ggcggtcgtt cattcgttca
     1621 agatcggagt agaatactgt ttcaaactac ctggtgtatt tattaatttt ggaactgtat
     1681 gtgtgtgtca tacatcttca tagttacgag tttaagatgg atggaaatat cgatctagga
     1741 taggtataca tgttgatgtg ggttttactg atgcatatac atgatggcat atgcagcatc
     1801 tattcatatg ctctaacctt gagtacctat ctattataat aaacaagtat gttttataat
     1861 tattttgatc ttgatatact tggatgatgg catatgcagc agctatatgt ggattttttt
     1921 agccctgcct tcatacgcta tttatttgct tggtactgtt tcttttgtcg atgctcaccc
     1981 tgttgtttgg tgttacttct gcagcctgca ggatggctcc taagaagaag cggaaggttg
     2041 gtattcacgg ggtgcctgcg gcttcaaagc tcgagaaatt caccaactgt tattcgttga
     2101 gcaaaacact gcggtttaaa gcgattccag tcggcaagac tcaagagaat atagacaata
     2161 agcggctgtt ggtggaagat gaaaagcgcg cggaagacta caaaggggtg aagaagttgt
     2221 tggacagata ctacctctct tttatcaatg atgtcttgca ctcaatcaaa ttgaagaatc
     2281 tgaacaacta catctccctc ttcagaaaga aaacaaggac agaaaaggag aataaggaac
     2341 ttgaaaattt ggagatcaat ctgaggaaag agatcgcgaa agcctttaaa ggcaacgaag
     2401 gatacaaaag tctgttcaag aaggatataa ttgagacaat tttgccagag ttcctcgatg
     2461 acaaggacga gattgcgctg gtcaattcgt tcaacggatt cacaacagca ttcacaggct
     2521 tctttgataa tcgggaaaat atgttctctg aggaggcaaa gtccacttct attgcgttca
     2581 ggtgtatcaa tgagaatctc actaggtaca tttccaacat ggatatcttt gagaaggttg
     2641 acgcaatttt tgacaagcac gaagttcagg agattaagga gaagatcctc aattccgatt
     2701 atgacgttga ggacttcttc gaaggtgagt tttttaattt cgtgctcact caagagggta
     2761 tcgacgtgta taatgcgatc atcggtgggt tcgtgactga gtccggtgaa aagattaagg
     2821 gattgaacga gtatatcaac ctttacaacc aaaagacgaa acagaagctg ccaaagttca
     2881 agcctcttta caaacaggtt ctttcagacc gcgagtcact ctcgttctat ggggagggct
     2941 acacttcgga tgaggaagtc ctggaggtgt tcaggaatac tctcaataag aattcggaga
     3001 ttttctcttc tataaaaaaa ctggaaaagt tgtttaagaa ttttgacgaa tactctagcg
     3061 ccggcatatt tgtgaaaaac ggcccggcca tatcaacgat aagtaaagat atcttcggcg
     3121 aatggaacgt gatcagagac aaatggaacg cggagtatga cgatattcac ctgaagaaga
     3181 aggctgtcgt aacggagaag tacgaggatg atcgcaggaa aagcttcaaa aagatcggaa
     3241 gtttcagcct ggaacagttg caggagtatg ctgacgccga tcttagcgtc gtcgagaagt
     3301 tgaaggagat aatcatccaa aaggtcgatg agatatataa agtctatgga tcaagtgaaa
     3361 aactgttcga cgccgacttc gttttggaga agtccctgaa gaagaacgac gctgttgttg
     3421 ccattatgaa ggatctgctc gacagcgtga agagtttcga gaactatatt aaggcttttt
     3481 tcggggaggg gaaggagact aacagagatg agtccttcta cggagacttc gtcctcgcgt
     3541 acgatatact ccttaaggta gaccacatct acgacgcaat cagaaattac gtgacacaaa
     3601 agccgtacag caaggacaag ttcaaactct acttccagaa cccccagttc atgggcggct
     3661 gggacaagga caaggaaacg gattacaggg ctacgatcct gaggtatggt tcaaaatact
     3721 acttggcgat tatggacaag aagtacgcca agtgtctcca gaagattgac aaagacgatg
     3781 tcaatggcaa ttatgagaag atcaactaca agctgcttcc gggtccgaac aagatgctcc
     3841 caaaggtttt cttcagcaag aaatggatgg cctactataa cccaagcgag gacatccaga
     3901 agatttataa gaacggtacg ttcaagaagg gcgacatgtt caatcttaac gactgtcaca
     3961 agctgatcga cttcttcaaa gactcaatta gccggtaccc aaagtggtct aacgcctatg
     4021 acttcaactt ttcggaaacc gagaagtaca aggatatagc cggattttat agagaggtgg
     4081 aagagcaggg ctacaaggtg tcattcgagt ccgccagcaa gaaggaagtg gacaagctcg
     4141 tggaagaggg taagctctac atgttccaga tttataataa agactttagc gataagagcc
     4201 acgggacacc taatctccac acaatgtatt tcaagctgct cttcgacgag aataaccacg
     4261 gccaaatcag gttgtcagga ggggctgaac tcttcatgcg gcgcgcatcc ctgaagaagg
     4321 aggagcttgt agtccaccct gcgaatagtc caattgcgaa taagaacccg gacaatccta
     4381 aaaagactac aacattgagc tacgacgtgt acaaggataa gaggttttcc gaggatcagt
     4441 acgagctcca catcccgatt gcgatcaaca agtgcccaaa gaatattttc aagataaaca
     4501 cagaggtgcg tgtactcctg aagcatgacg acaatcctta cgtcattggg attgatcggg
     4561 gcgagaggaa cctcctctat attgtggtgg tggacgggaa ggggaacata gtcgaacagt
     4621 actcccttaa cgaaataatt aacaatttca acggcatccg tatcaagacc gactaccatt
     4681 cgttgctgga caagaaggag aaggagagat ttgaggcgcg gcaaaattgg acaagtatcg
     4741 agaacatcaa ggaactcaaa gcaggttata tctctcaagt tgtgcataag atatgcgagc
     4801 tggttgagaa gtatgacgca gtgatcgctc ttgaggacct caactcgggc tttaagaata
     4861 gccgcgttaa agtggagaag caggtctatc aaaagttcga gaagatgctt atagataagc
     4921 tcaactacat ggtcgataag aaatcgaacc catgtgccac cggcggcgca ctcaaaggtt
     4981 accaaataac aaacaaattc gagtccttca aatcgatgag tactcagaat gggttcatat
     5041 tttatatacc ggcgtggctt acgtctaaga tcgacccgtc aactggtttt gtcaacctgt
     5101 tgaagacgaa atacacgtcc attgccgatt cgaaaaagtt catatctagt tttgatcgta
     5161 ttatgtacgt cccagaggaa gatcttttcg agtttgctct cgactacaaa aacttttcgc
     5221 ggaccgatgc ggattacatt aaaaaatgga aactctattc gtacggcaac agaatcagga
     5281 tttttcgcaa ccctaagaag aataacgtct ttgattggga ggaagtttgc ttgactagcg
     5341 cgtacaagga gctctttaat aagtatggca ttaactacca acagggtgat atcagagcac
     5401 tgctttgcga acaatctgac aaggctttct actcatcctt catggctttg atgagcctga
     5461 tgctccagat gagaaattca attacaggca gaaccgacgt ggatttcttg atctccccgg
     5521 ttaaaaattc tgatggcatc ttttacgata gcaggaacta tgaagcgcaa gagaatgcga
     5581 ttctgccaaa aaatgcagac gccaacggtg cctataacat cgccaggaaa gtcctgtggg
     5641 cgatcggcca gttcaaaaag gccgaagacg aaaaattgga caaggtcaaa atcgctatca
     5701 gcaacaaaga gtggctggag tatgctcaga catccgtaaa gcataagcgt cctgctgcca
     5761 ccaaaaaggc cggacaggct aagaaaaaga agtgaaaaaa aaaaaaaaaa aaaaaaaaaa
     5821 aaaaaaaaaa aaaaaaaaaa aaaaaggaga ccttatattc cccagaacat caggttaatg
     5881 gcgtttttga tgtcattttc gcggtggctg agatcagcca cttcttcccc gataacggaa
     5941 accggcacac tggccatatc ggtggtcatc atgcgccagc tttcatcccc gatatgcacc
     6001 accgggtaaa gttcacggga gactttatct gacagcagac gtgcactggc cagggggatc
     6061 accatccgtc gcccgggcgt gtcaataata tcactctgta catccacaaa cagacgataa
     6121 cggctctctc ttttataggt gtaaacctta aactgcattt caccagcccc tgttctcgtc
     6181 agcaaaagag ccgttcattt caataaaccg ggcgacctca gccatccctt cctgattttc
     6241 cgctttccag cgttcggcac gcagacgacg ggcttcattc tgcatggttg tgcttaccag
     6301 accggagata ttgacatcat atatgccttg agcaactgat agctgtcgct gtcaactgtc
     6361 actgtaatac gctgcttcat agcatacctc tttttgacat acttcgggta tacatatcag
     6421 tatatattct tataccgcaa aaatcagcgc gcaaatacgc atactgttat ctggcttggt
     6481 ctcgaatttc tactaagtgt agatggtacc cggccggaat tctagaatat gaagatgaag
     6541 atgaaatatt tggtgtgtca aataaaaagc ttgtgtgctt aagtttgtgt ttttttcttg
     6601 gcttgttgtg ttatgaattt gtggcttttt ctaatattaa atgaatgtaa gatcacatta
     6661 taatgaataa acaaatgttt ctataatcca ttgtgaatgt tttgttggat ctcttctgca
     6721 gcatataact actgtatgtg ctatggtatg gactatggaa tatgattaaa gataaggagc
     6781 tcaaactatc agtgtttgac aggatatatt ggcgggtaaa cctaagagaa aagagcgttt
     6841 attagaataa tcggatattt aaaagggcgt gaaaaggttt atccgttcgt ccatttgtat
     6901 gtgcatgcca accacagggt tcccctcggg atcaaagtac tttgatccaa cccctccgct
     6961 gctatagtgc agtcggcttc tgacgttcag tgcagccgtc ttctgaaaac gacatgtcgc
     7021 acaagtccta agttacgcga caggctgccg ccctgccctt ttcctggcgt tttcttgtcg
     7081 cgtgttttag tcgcataaag tagaatactt gcgactagaa ccggagacat tacgccatga
     7141 acaagagcgc cgccgctggc ctgctgggct atgcccgcgt cagcaccgac gaccaggact
     7201 tgaccaacca acgggccgaa ctgcacgcgg ccggctgcac caagctgttt tccgagaaga
     7261 tcaccggcac caggcgcgac cgcccggagc tggccaggat gcttgaccac ctacgccctg
     7321 gcgacgttgt gacagtgacc aggctagacc gcctggcccg cagcacccgc gacctactgg
     7381 acattgccga gcgcatccag gaggccggcg cgggcctgcg tagcctggca gagccgtggg
     7441 ccgacaccac cacgccggcc ggccgcatgg tgttgaccgt gttcgccggc attgccgagt
     7501 tcgagcgttc cctaatcatc gaccgcaccc ggagcgggcg cgaggccgcc aaggcccgag
     7561 gcgtgaagtt tggcccccgc cctaccctca ccccggcaca gatcgcgcac gcccgcgagc
     7621 tgatcgacca ggaaggccgc accgtgaaag aggcggctgc actgcttggc gtgcatcgct
     7681 cgaccctgta ccgcgcactt gagcgcagcg aggaagtgac gcccaccgag gccaggcggc
     7741 gcggtgcctt ccgtgaggac gcattgaccg aggccgacgc cctggcggcc gccgagaatg
     7801 aacgccaaga ggaacaagca tgaaaccgca ccaggacggc caggacgaac cgtttttcat
     7861 taccgaagag atcgaggcgg agatgatcgc ggccgggtac gtgttcgagc cgcccgcgca
     7921 cggctcaacc gtgcggctgc atgaaatcct ggccggtttg tctgatgcca agctggcggc
     7981 ctggccggcc agcttggccg ctgaagaaac cgagcgccgc cgtctaaaaa ggtgatgtgt
     8041 atttgagtaa aacagcttgc gtcatgcggt cgctgcgtat atgatgcgat gagtaaataa
     8101 acaaatacgc aaggggaacg catgaaggtt atcgctgtac ttaaccagaa aggcgggtca
     8161 ggcaagacga ccatcgcaac ccatctagcc cgcgccctgc aactcgccgg ggccgatgtt
     8221 ctgttagtcg attccgatcc ccagggcagt gcccgcgatt gggcggccgt gcgggaagat
     8281 caaccgctaa ccgttgtcgg catcgaccgc ccgacgattg accgcgacgt gaaggccatc
     8341 ggccggcgcg acttcgtagt gatcgacgga gcgccccagg cggcggactt ggctgtgtcc
     8401 gcgatcaagg cagccgactt cgtgctgatt ccggtgcagc caagccctta cgacatatgg
     8461 gccaccgccg acctggtgga gctggttaag cagcgcattg aggtcacgga tggaaggcta
     8521 caagcggcct ttgtcgtgtc gcgggcgatc aaaggcacgc gcatcggcgg tgaggttgcc
     8581 gaggcgctgg ccgggtacga gctgcccatt cttgagtccc gtatcacgca gcgcgtgagc
     8641 tacccaggca ctgccgccgc cggcacaacc gttcttgaat cagaacccga gggcgacgct
     8701 gcccgcgagg tccaggcgct ggccgctgaa attaaatcaa aactcatttg agttaatgag
     8761 gtaaagagaa aatgagcaaa agcacaaaca cgctaagtgc cggccgtccg agcgcacgca
     8821 gcagcaaggc tgcaacgttg gccagcctgg cagacacgcc agccatgaag cgggtcaact
     8881 ttcagttgcc ggcggaggat cacaccaagc tgaagatgta cgcggtacgc caaggcaaga
     8941 ccattaccga gctgctatct gaatacatcg cgcagctacc agagtaaatg agcaaatgaa
     9001 taaatgagta gatgaatttt agcggctaaa ggaggcggca tggaaaatca agaacaacca
     9061 ggcaccgacg ccgtggaatg ccccatgtgt ggaggaacgg gcggttggcc aggcgtaagc
     9121 ggctgggttg tctgccggcc ctgcaatggc actggaaccc ccaagcccga ggaatcggcg
     9181 tgacggtcgc aaaccatccg gcccggtaca aatcggcgcg gcgctgggtg atgacctggt
     9241 ggagaagttg aaggccgcgc aggccgccca gcggcaacgc atcgaggcag aagcacgccc
     9301 cggtgaatcg tggcaagcgg ccgctgatcg aatccgcaaa gaatcccggc aaccgccggc
     9361 agccggtgcg ccgtcgatta ggaagccgcc caagggcgac gagcaaccag attttttcgt
     9421 tccgatgctc tatgacgtgg gcacccgcga tagtcgcagc atcatggacg tggccgtttt
     9481 ccgtctgtcg aagcgtgacc gacgagctgg cgaggtgatc cgctacgagc ttccagacgg
     9541 gcacgtagag gtttccgcag ggccggccgg catggccagt gtgtgggatt acgacctggt
     9601 actgatggcg gtttcccatc taaccgaatc catgaaccga taccgggaag ggaagggaga
     9661 caagcccggc cgcgtgttcc gtccacacgt tgcggacgta ctcaagttct gccggcgagc
     9721 cgatggcgga aagcagaaag acgacctggt agaaacctgc attcggttaa acaccacgca
     9781 cgttgccatg cagcgtacga agaaggccaa gaacggccgc ctggtgacgg tatccgaggg
     9841 tgaagccttg attagccgct acaagatcgt aaagagcgaa accgggcggc cggagtacat
     9901 cgagatcgag ctagctgatt ggatgtaccg cgagatcaca gaaggcaaga acccggacgt
     9961 gctgacggtt caccccgatt actttttgat cgatcccggc atcggccgtt ttctctaccg
    10021 cctggcacgc cgcgccgcag gcaaggcaga agccagatgg ttgttcaaga cgatctacga
    10081 acgcagtggc agcgccggag agttcaagaa gttctgtttc accgtgcgca agctgatcgg
    10141 gtcaaatgac ctgccggagt acgatttgaa ggaggaggcg gggcaggctg gcccgatcct
    10201 agtcatgcgc taccgcaacc tgatcgaggg cgaagcatcc gccggttcct aatgtacgga
    10261 gcagatgcta gggcaaattg ccctagcagg ggaaaaaggt cgaaaaggcc tctttcctgt
    10321 ggatagcacg tacattggga acccaaagcc gtacattggg aaccggaacc cgtacattgg
    10381 gaacccaaag ccgtacattg ggaaccggtc acacatgtaa gtgactgata taaaagagaa
    10441 aaaaggcgat ttttccgcct aaaactcttt aaaacttatt aaaactctta aaacccgcct
    10501 ggcctgtgca taactgtctg gccagcgcac agccgaagag ctgcaaaaag cgcctaccct
    10561 tcggtcgctg cgctccctac gccccgccgc ttcgcgtcgg cctatcgcgg ccgctggccg
    10621 ctcaaaaatg gctggcctac ggccaggcaa tctaccaggg cgcggacaag ccgcgccgtc
    10681 gccactcgac cgccggcgcc cacatcaagg caccctgcct cgcgcgtttc ggtgatgacg
    10741 gtgaaaacct ctgacacatg cagctcccgg aaacggtcac agcttgtctg taagcggatg
    10801 ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt cggggcgcag
    10861 ccatgaccca gtcacgtagc gatagcggag tgtatactgg cttaactatg cggcatcaga
    10921 gcagattgta ctgagagtgc accatatgcg gtgtgaaata ccgcacagat gcgtaaggag
    10981 aaaataccgc atcaggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt
    11041 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc
    11101 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa
    11161 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
    11221 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
    11281 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
    11341 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag
    11401 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
    11461 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
    11521 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
    11581 agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat ttggtatctg
    11641 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
    11701 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
    11761 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
    11821 ctcacgttaa gggattttgg tcatgcattc taggtactaa aacaattcat ccagtaaaat
    11881 ataatatttt attttctccc aatcaggctt gatccccagt aagtcaaaaa atagctcgac
    11941 atactgttct tccccgatat cctccctgat cgaccggacg cagaaggcaa tgtcatacca
    12001 cttgtccgcc ctgccgcttc tcccaagatc aataaagcca cttactttgc catctttcac
    12061 aaagatgttg ctgtctccca ggtcgccgtg ggaaaagaca agttcctctt cgggcttttc
    12121 cgtctttaaa aaatcataca gctcgcgcgg atctttaaat ggagtgtctt cttcccagtt
    12181 ttcgcaatcc acatcggcca gatcgttatt cagtaagtaa tccaattcgg ctaagcggct
    12241 gtctaagcta ttcgtatagg gacaatccga tatgtcgatg gagtgaaaga gcctgatgca
    12301 ctccgcatac agctcgataa tcttttcagg gctttgttca tcttcatact cttccgagca
    12361 aaggacgcca tcggcctcac tcatgagcag attgctccag ccatcatgcc gttcaaagtg
    12421 caggaccttt ggaacaggca gctttccttc cagccatagc atcatgtcct tttcccgttc
    12481 cacatcatag gtggtccctt tataccggct gtccgtcatt tttaaatata ggttttcatt
    12541 ttctcccacc agcttatata ccttagcagg agacattcct tccgtatctt ttacgcagcg
    12601 gtatttttcg atcagttttt tcaattccgg tgatattctc attttagcca tttattattt
    12661 ccttcctctt ttctacagta tttaaagata ccccaagaag ctaattataa caagacgaac
    12721 tccaattcac tgttccttgc attctaaaac cttaaatacc agaaaacagc tttttcaaag
    12781 ttgttttcaa agttggcgta taacatagta tcgacggagc cgattttgaa accgcggtga
    12841 tcacaggcag caacgctctg tcatcgttac aatcaacatg ctaccctccg cgagatcatc
    12901 cgtgtttcaa acccggcagc ttagttgccg ttcttccgaa tagcatcggt aacatgagca
    12961 aagtctgccg ccttacaacg gctctcccgc tgacgccgtc ccggactgat gggctgcctg
    13021 tatcgagtgg tgattttgtg ccgagctgcc ggtcggggag ctgttggctg gctggtggca
    13081 ggatatattg tggtgtaaac aaattgacgc ttagacaact taataacaca ttgcggacgt
    13141 ttttaatgta ctgaattaac gccgaattaa ttcgggggat ctggatttta gtactggatt
    13201 ttggttttag gaattagaaa ttttattgat agaagtattt tacaaataca aatacatact
    13261 aagggtttct tatatgctca acacatgagc gaaaccctat aggaacccta attcccttat
    13321 ctgggaacta ctcacacatt attatggaga aaatagagct tgtcgatcgt taattaatca
    13381 cctccaagat cccggacgag tgctggggcg tcggtttcca ctatcggcga gtacttctac
    13441 acagccatcg gtccagacgg ccgcgcttct gcgggcgatt tgtgtacgcc cgacagtccc
    13501 ggctccggat cggacgattg cgtcgcatcg accctgcgcc caagctgcat catcgaaatt
    13561 gccgtcaacc aagctctgat agagttggtc aagaccaatg cggagcatat acgcccggag
    13621 ccgcggcgat cctgcaagct ccggatgcct ccgctcgaag tagcgcgtct gctgctccat
    13681 acaagccaac cacggcctcc agaagaagat gttggcgacc tcgtattggg aatccccgaa
    13741 catcgcctcg ctccagtcaa tgaccgctgt tatgcggcca ttgtccgtca ggacattgtt
    13801 ggagccgaaa tccgcgtgca cgaggtgccg gacttcgggg cagtcctcgg cccaaagcat
    13861 cagctcatcg agagcctgcg cgacggacgc actgacggtg tcgtccatca cagtttgcca
    13921 gtgatacaca tggggatcag caatcgcgca tatgaaatca cgccatgtag tgtattgacc
    13981 gattccttgc ggtccgaatg ggccgaaccc gctcgtctgg ctaagatcgg ccgcagcgat
    14041 cgcatccatg gcctccgcga ccggctgcag aacagcgggc agttcggttt caggcaggtc
    14101 ttgcaacgtg acaccctgtg cacggcggga gatgcaatag gtcaggctct cgctgaattc
    14161 cccaatgtca agcacttccg gaatcgggag cgcggccgat gcaaagtgcc gataaacata
    14221 acgatctttg tagaaaccat cggcgcagct atttacccgc aggacatatc cacgccctcc
    14281 tacatcgaag ctgaaagcac gagattcttc gccctccgag agctgcatca ggtcggacac
    14341 gctgtcgaac ttttcgatca gaaacttctc gacagacgtc gcggtgagtt caggcttttt
    14401 catcctagga gagatagatt tgtagagaga gactggtgat ttcagcgtgt cctctccaaa
    14461 tgaaatgaac ttccttatat agaggaaggt cttgcgaagg atagtgggat tgtgcgtcat
    14521 cccttacgtc agtggagata tcacatcaat ccacttgctt tgaagacgtg gttggaacgt
    14581 cttctttttc cacgatgctc ctcgtgggtg ggggtccatc tttgggacca ctgtcggcag
    14641 aggcatcttg aacgatagcc tttcctttat cgcaatgatg gcatttgtag gtgccacctt
    14701 ccttttctac tgtccttttg atgaagtgac agatagctgg gcaatggaat ccgaggaggt
    14761 ttcccgatat taccctttgt tgaaaagtct caatagccct ttggtcttct gagactgtat
    14821 ctttgatatt cttggagtag acgagagtgt cgtgctccac catgttatca catcaatcca
    14881 cttgctttga agacgtggtt ggaacgtctt ctttttccac gatgctcctc gtgggtgggg
    14941 gtccatcttt gggaccactg tcggcagagg catcttgaac gatagccttt cctttatcgc
    15001 aatgatggca tttgtaggtg ccaccttcct tttctactgt ccttttgatg aagtgacaga
    15061 tagctgggca atggaatccg aggaggtttc ccgatattac cctttgttga aaagtctcaa
    15121 tagccctttg gtcttctgag actgtatctt tgatattctt ggagtagacg agagtgtcgt
    15181 gctccaccat g
//
