LOCUS       sequence-266289-       16675 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  OsBiP1 promoter driven Cas9-tRNA system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16675)
  AUTHORS   Ren Q, Zhong Z, Wang Y, You Q, Li Q, Yuan M, He Y, Qi C, Tang X, 
            Zheng X, Zhang T, Qi Y, Zhang Y
  TITLE     Bidirectional Promoter-Based CRISPR-Cas9 Systems for Plant Genome 
            Editing.
  JOURNAL   Front Plant Sci. 2019 Sep 20;10:1173. doi: 10.3389/fpls.2019.01173. 
            eCollection 2019.
  PUBMED    31616455
REFERENCE   2  (bases 1 to 16675)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front Plant
            Sci."; date: "2019-09-20"; volume: "10:1173. doi"; pages: " 
            10.3389/fpls.2019.01173. eCollection 2019"
COMMENT     Sequence Label: pGEL052
FEATURES             Location/Qualifiers
     source          1..16675
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(16..723)
                     /label=OCS terminator
                     /note="octopine synthase terminator"
     misc_RNA        complement(807..882)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     CDS             1209..1514
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     1393..1412
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     CDS             3866..7969
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             3866..7969
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             7982..8002
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     terminator      8013..8261
                     /label=HSP terminator
                     /note="efficient transcription terminator from the 
                     Arabidopsis thaliana heat shock protein 18.2 gene (Nagaya 
                     et al., 2010)"
     misc_feature    8282..8306
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             9606..10235
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             10669..11736
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQA
                     AADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKR
                     DRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGR
                     VFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEAL
                     ISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLA
                     RRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILV
                     MRYRNLIEGEASAGS"
     rep_origin      11802..11996
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(12232..12254)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    12340..12480
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     12395..12414
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(12495..12512)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(12666..13254)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(12746..12765)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(13341..14135)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    14560..14584
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    14662..14836
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     promoter        complement(15926..16603)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
ORIGIN
        1 ggcgcgccgc cggtcctgct gagcctcgac atgttgtcgc aaaattcgcc ctggacccgc
       61 ccaacgattt gtcgtcactg tcaaggtttg acctgcactt catttggggc ccacatacac
      121 caaaaaaatg ctgcataatt ctcggggcag caagtcggtt acccggccgc cgtgctggac
      181 cgggttgaat ggtgcccgta actttcggta gagcggacgg ccaatactca acttcaagga
      241 atctcaccca tgcgcgccgg cggggaaccg gagttccctt cagtgagcgt tattagttcg
      301 ccgctcggtg tgtcgtagat actagcccct ggggcacttt tgaaatttga ataagattta
      361 tgtaatcagt cttttaggtt tgaccggttc tgccgctttt tttaaaattg gatttgtaat
      421 aataaaacgc aattgtttgt tattgtggcg ctctatcata gatgtcgcta taaacctatt
      481 cagcacaata tattgttttc attttaatat tgtacatata agtagtaggg tacaatcagt
      541 aaattgaacg gagaatatta ttcataaaaa tacgatagta acgggtgata tattcattag
      601 aatgaaccga aaccggcggt aaggatctga gctacacatg ctcaggtttt ttacaacgtg
      661 cacaacagaa ttgaaagcaa atatcatgcg atcataggcg tctcgcatat ctcattaaag
      721 cagggtacct gcaccagccg ggaatcgaac ccgggtctgt accgtggcag ggtactattc
      781 taccactaga ccactggtgc tttgttgcac cgactcggtg ccactttttc aagttgataa
      841 cggactagcc ttattttaac ttgctatttc tagctctaaa actgagacca agccagataa
      901 cagtatgcgt atttgcgcgc tgatttttgc ggtataagaa tatatactga tatgtatacc
      961 cgaagtatgt caaaaagagg tatgctatga agcagcgtat tacagtgaca gttgacagcg
     1021 acagctatca gttgctcaag gcatatatga tgtcaatatc tccggtctgg taagcacaac
     1081 catgcagaat gaagcccgtc gtctgcgtgc cgaacgctgg aaagcggaaa atcaggaagg
     1141 gatggctgag gtcgcccggt ttattgaaat gaacggctct tttgctgacg agaacagggg
     1201 ctggtgaaat gcagtttaag gtttacacct ataaaagaga gagccgttat cgtctgtttg
     1261 tggatgtaca gagtgatatt attgacacgc ccgggcgacg gatggtgatc cccctggcca
     1321 gtgcacgtct gctgtcagat aaagtctccc gtgaacttta cccggtggtg catatcgggg
     1381 atgaaagctg gcgcatgatg accaccgata tggccagtgt gccggtttcc gttatcgggg
     1441 aagaagtggc tgatctcagc caccgcgaaa atgacatcaa aaacgccatt aacctgatgt
     1501 tctggggaat ataaggtctc ctgcaccagc cgggaatcga acccgggtct gtaccgtggc
     1561 agggtactat tctaccacta gaccactggt gctttgttac tagtagctgc aaacataaca
     1621 aatatacctc agaatcaata aactaggcta aaagggaact gaagagaatc aacagactgt
     1681 agtctctgag cagtaactac acaaaaacat atgatagaac aatctggtaa aaacatatgc
     1741 agtatgttct acaccacaaa tttattacat acaacaatta catctatcag cgtgaagaac
     1801 gcatgtcaaa tagtttcttt caatttttca tacaccaaaa caaaattcat gggctaaaac
     1861 cccaagactt taaattgcat aaatactgac aagtggcaac aaaagctgat tttaagggta
     1921 tgtaaaccta ccagtaaggt ggacgttgac agggcaaaca aattagaatt ttgcactgat
     1981 actaaaatgg gcttccaaac cctaataggc actagatcag actgcacaca catacaacta
     2041 ctaatgggaa aactaggtca gcatatacat tcaacgccgt aaattggcat agaaaacttc
     2101 atcatcacag atgaaaccaa gcagcttgta tttcttatgt ggttataaag gggagcactt
     2161 gccattgctg ttacaagaca tacatttatg tagattaata atgattttcc atggaatcca
     2221 caagcatttg taagcgaaaa cgaagcaaac attacaaaat tcccaagtat cactaaagca
     2281 gaatagcaga tgcaagtaac atggcaccta ttagtaacct aagccgtagc aaacttcaca
     2341 ctccacgtct ccagaacaaa taaaagctct gcaacgacct aaagttatgc gaaaagtgca
     2401 aaatccccaa caagcgccac ttcgtcatgt gaaccgaacc agccaaaatc tcaaacctcc
     2461 cctccccttc tcgattctca taacgcaata gaagtataga gaagggattg cttcacggga
     2521 cgcacctttg atcccgaggg acgtctggcc ggatccgacc gcggtgagcg cgtgctcgat
     2581 ctgcaccagc ttccccgacg ggctgcaaac gaaatcgcca caaaaaccac agatgacgaa
     2641 accgaaaccg caccagatca cgcccgatct ccgggcgagg gaggcgcaag gcagcacata
     2701 cctgaaggtg gtgagggaga aggagtactg gctgtctccc atggccgccg acgcgcaggc
     2761 ctacctgtga gctgctgctc ttcgtgtctc gcggcggaag aggaggcgac cgagcgagcg
     2821 attgcggcga gacggcgtgg gaagggagag ggaggcagag cacaactcgc caaggaagaa
     2881 gaggagagag agaaagagac tggatggatg gattgctccg caagaaggtg gtgggtcttg
     2941 ggccggaaat ctggcccatc tgttattggg tcagcccatg actcacccac ctccccgtca
     3001 cgtcgtcttc ctcctcataa cttcttcccc acgggccaac ggctgcgcgt cttcccgttc
     3061 gtcctcctgt ccggtccgag tccctctgac gacgcgactt tccccacgcc gggatcgagc
     3121 tccggggagg ggagctgcga gctggtacgt acgcttgatt tcttcccttg ccaccgcgat
     3181 attccgatgt atccgtgatg tgtcgttcat gggtagatcg gctagggatt tggcggcgtt
     3241 gaggaaagcg atggctgccg ggtgggggga attgttgtat tagactctcg aagctgatat
     3301 ctggtgtgga tttgcggtgg aagccatggt cggaggccca gtgccttccg ggagttgttg
     3361 ggttatcggg agcccggagg gcagtgcggc tcgccgactg ctgccatgat ccccgtcggc
     3421 aaaatctcca gattggggga gatggggggt aggaaccatt tggattagta gtgtatacac
     3481 ggggtttcgt ttattatatt tagagcgctc tacatgtaga cagcacacag cagctttaac
     3541 gtatggatgg tgtatcgcag ggaatgtatc tcgaagacat tggattaggc tattattaca
     3601 tttagagaac cagattgggt ttgcgataac tagaaattga gtctgttatg aatttattga
     3661 tggagcggac agggaattac gattactatc tgtttagctt ttcagttgtg aatgatgatt
     3721 atttcaattt agctaatcaa ggttcctcaa tcaaaccgtt ctaacttttt ctatttatgc
     3781 tcttggttac attttgacag aaacactttg tcatatcagt caacagtaga gaggagacga
     3841 gcctgcaggt agatcgctcg tcgacatgga taagaagtac tctatcggac tcgatatcgg
     3901 aactaactct gtgggatggg ctgtgatcac cgatgagtac aaggtgccat ctaagaagtt
     3961 caaggttctc ggaaacaccg ataggcactc tatcaagaaa aaccttatcg gtgctctcct
     4021 cttcgattct ggtgaaactg ctgaggctac cagactcaag agaaccgcta gaagaaggta
     4081 caccagaaga aagaacagga tctgctacct ccaagagatc ttctctaacg agatggctaa
     4141 agtggatgat tcattcttcc acaggctcga agagtcattc ctcgtggaag aagataagaa
     4201 gcacgagagg caccctatct tcggaaacat cgttgatgag gtggcatacc acgagaagta
     4261 ccctactatc taccacctca gaaagaagct cgttgattct actgataagg ctgatctcag
     4321 gctcatctac ctcgctctcg ctcacatgat caagttcaga ggacacttcc tcatcgaggg
     4381 tgatctcaac cctgataact ctgatgtgga taagttgttc atccagctcg tgcagaccta
     4441 caaccagctt ttcgaagaga accctatcaa cgcttcaggt gtggatgcta aggctatcct
     4501 ctctgctagg ctctctaagt caagaaggct tgagaacctc attgctcagc tccctggtga
     4561 gaagaagaac ggacttttcg gaaacttgat cgctctctct ctcggactca cccctaactt
     4621 caagtctaac ttcgatctcg ctgaggatgc aaagctccag ctctcaaagg atacctacga
     4681 tgatgatctc gataacctcc tcgctcagat cggagatcag tacgctgatt tgttcctcgc
     4741 tgctaagaac ctctctgatg ctatcctcct cagtgatatc ctcagagtga acaccgagat
     4801 caccaaggct ccactctcag cttctatgat caagagatac gatgagcacc accaggatct
     4861 cacacttctc aaggctcttg ttagacagca gctcccagag aagtacaaag agattttctt
     4921 cgatcagtct aagaacggat acgctggtta catcgatggt ggtgcatctc aagaagagtt
     4981 ctacaagttc atcaagccta tcctcgagaa gatggatgga accgaggaac tcctcgtgaa
     5041 gctcaataga gaggatcttc tcagaaagca gaggaccttc gataacggat ctatccctca
     5101 tcagatccac ctcggagagt tgcacgctat ccttagaagg caagaggatt tctacccatt
     5161 cctcaaggat aacagggaaa agattgagaa gattctcacc ttcagaatcc cttactacgt
     5221 gggacctctc gctagaggaa actcaagatt cgcttggatg accagaaagt ctgaggaaac
     5281 catcacccct tggaacttcg aagaggtggt ggataagggt gctagtgctc agtctttcat
     5341 cgagaggatg accaacttcg ataagaacct tccaaacgag aaggtgctcc ctaagcactc
     5401 tttgctctac gagtacttca ccgtgtacaa cgagttgacc aaggttaagt acgtgaccga
     5461 gggaatgagg aagcctgctt ttttgtcagg tgagcaaaag aaggctatcg ttgatctctt
     5521 gttcaagacc aacagaaagg tgaccgtgaa gcagctcaaa gaggattact tcaagaaaat
     5581 cgagtgcttc gattcagttg agatttctgg tgttgaggat aggttcaacg catctctcgg
     5641 aacctaccac gatctcctca agatcattaa ggataaggat ttcttggata acgaggaaaa
     5701 cgaggatatc ttggaggata tcgttcttac cctcaccctc tttgaagata gagagatgat
     5761 tgaagaaagg ctcaagacct acgctcatct cttcgatgat aaggtgatga agcagttgaa
     5821 gagaagaaga tacactggtt ggggaaggct ctcaagaaag ctcattaacg gaatcaggga
     5881 taagcagtct ggaaagacaa tccttgattt cctcaagtct gatggattcg ctaacagaaa
     5941 cttcatgcag ctcatccacg atgattctct cacctttaaa gaggatatcc agaaggctca
     6001 ggtttcagga cagggtgata gtctccatga gcatatcgct aacctcgctg gatctcctgc
     6061 aatcaagaag ggaatcctcc agactgtgaa ggttgtggat gagttggtga aggtgatggg
     6121 aaggcataag cctgagaaca tcgtgatcga aatggctaga gagaaccaga ccactcagaa
     6181 gggacagaag aactctaggg aaaggatgaa gaggatcgag gaaggtatca aagagcttgg
     6241 atctcagatc ctcaaagagc accctgttga gaacactcag ctccagaatg agaagctcta
     6301 cctctactac ctccagaacg gaagggatat gtatgtggat caagagttgg atatcaacag
     6361 gctctctgat tacgatgttg atcatatcgt gccacagtca ttcttgaagg atgattctat
     6421 cgataacaag gtgctcacca ggtctgataa gaacaggggt aagagtgata acgtgccaag
     6481 tgaagaggtt gtgaagaaaa tgaagaacta ttggaggcag ctcctcaacg ctaagctcat
     6541 cactcagaga aagttcgata acttgactaa ggctgagagg ggaggactct ctgaattgga
     6601 taaggcagga ttcatcaaga ggcagcttgt ggaaaccagg cagatcacta agcacgttgc
     6661 acagatcctc gattctagga tgaacaccaa gtacgatgag aacgataagt tgatcaggga
     6721 agtgaaggtt atcaccctca agtcaaagct cgtgtctgat ttcagaaagg atttccaatt
     6781 ctacaaggtg agggaaatca acaactacca ccacgctcac gatgcttacc ttaacgctgt
     6841 tgttggaacc gctctcatca agaagtatcc taagctcgag tcagagttcg tgtacggtga
     6901 ttacaaggtg tacgatgtga ggaagatgat cgctaagtct gagcaagaga tcggaaaggc
     6961 taccgctaag tatttcttct actctaacat catgaatttc ttcaagaccg agattaccct
     7021 cgctaacggt gagatcagaa agaggccact catcgagaca aacggtgaaa caggtgagat
     7081 cgtgtgggat aagggaaggg atttcgctac cgttagaaag gtgctctcta tgccacaggt
     7141 gaacatcgtt aagaaaaccg aggtgcagac cggtggattc tctaaagagt ctatcctccc
     7201 taagaggaac tctgataagc tcattgctag gaagaaggat tgggacccta agaaatacgg
     7261 tggtttcgat tctcctaccg tggcttactc tgttctcgtt gtggctaagg ttgagaaggg
     7321 aaagagtaag aagctcaagt ctgttaagga acttctcgga atcactatca tggaaaggtc
     7381 atctttcgag aagaacccaa tcgatttcct cgaggctaag ggatacaaag aggttaagaa
     7441 ggatctcatc atcaagctcc caaagtactc actcttcgaa ctcgagaacg gtagaaagag
     7501 gatgctcgct tctgctggtg agcttcaaaa gggaaacgag cttgctctcc catctaagta
     7561 cgttaacttt ctttacctcg cttctcacta cgagaagttg aagggatctc cagaagataa
     7621 cgagcagaag caacttttcg ttgagcagca caagcactac ttggatgaga tcatcgagca
     7681 gatctctgag ttctctaaaa gggtgatcct cgctgatgca aacctcgata aggtgttgtc
     7741 tgcttacaac aagcacagag ataagcctat cagggaacag gcagagaaca tcatccatct
     7801 cttcaccctt accaacctcg gtgctcctgc tgctttcaag tacttcgata caaccatcga
     7861 taggaagaga tacacctcta ccaaagaagt gctcgatgct accctcatcc atcagtctat
     7921 cactggactc tacgagacta ggatcgatct ctcacagctc ggtggtgatt caagggctga
     7981 tcctaagaag aagaggaagg tttgaggatc catatgaaga tgaagatgaa atatttggtg
     8041 tgtcaaataa aaagcttgtg tgcttaagtt tgtgtttttt tcttggcttg ttgtgttatg
     8101 aatttgtggc tttttctaat attaaatgaa tgtaagatca cattataatg aataaacaaa
     8161 tgtttctata atccattgtg aatgttttgt tggatctctt ctgcagcata taactactgt
     8221 atgtgctatg gtatggacta tggaatatga ttaaagataa ggagctcaaa ctatcagtgt
     8281 ttgacaggat atattggcgg gtaaacctaa gagaaaagag cgtttattag aataacggat
     8341 atttaaaagg gcgtgaaaag gtttatccgt tcgtccattt gtatgtgcat gccaaccaca
     8401 gggttcccct cgggatcaaa gtactttgat ccaacccctc cgctgctata gtgcagtcgg
     8461 cttctgacgt tcagtgcagc cgtcttctga aaacgacatg tcgcacaagt cctaagttac
     8521 gcgacaggct gccgccctgc ccttttcctg gcgttttctt gtcgcgtgtt ttagtcgcat
     8581 aaagtagaat acttgcgact agaaccggag acattacgcc atgaacaaga gcgccgccgc
     8641 tggcctgctg ggctatgccc gcgtcagcac cgacgaccag gacttgacca accaacgggc
     8701 cgaactgcac gcggccggct gcaccaagct gttttccgag aagatcaccg gcaccaggcg
     8761 cgaccgcccg gagctggcca ggatgcttga ccacctacgc cctggcgacg ttgtgacagt
     8821 gaccaggcta gaccgcctgg cccgcagcac ccgcgaccta ctggacattg ccgagcgcat
     8881 ccaggaggcc ggcgcgggcc tgcgtagcct ggcagagccg tgggccgaca ccaccacgcc
     8941 ggccggccgc atggtgttga ccgtgttcgc cggcattgcc gagttcgagc gttccctaat
     9001 catcgaccgc acccggagcg ggcgcgaggc cgccaaggcc cgaggcgtga agtttggccc
     9061 ccgccctacc ctcaccccgg cacagatcgc gcacgcccgc gagctgatcg accaggaagg
     9121 ccgcaccgtg aaagaggcgg ctgcactgct tggcgtgcat cgctcgaccc tgtaccgcgc
     9181 acttgagcgc agcgaggaag tgacgcccac cgaggccagg cggcgcggtg ccttccgtga
     9241 ggacgcattg accgaggccg acgccctggc ggccgccgag aatgaacgcc aagaggaaca
     9301 agcatgaaac cgcaccagga cggccaggac gaaccgtttt tcattaccga agagatcgag
     9361 gcggagatga tcgcggccgg gtacgtgttc gagccgcccg cgcacggctc aaccgtgcgg
     9421 ctgcatgaaa tcctggccgg tttgtctgat gccaagctgg cggcctggcc ggccagcttg
     9481 gccgctgaag aaaccgagcg ccgccgtcta aaaaggtgat gtgtatttga gtaaaacagc
     9541 ttgcgtcatg cggtcgctgc gtatatgatg cgatgagtaa ataaacaaat acgcaagggg
     9601 aacgcatgaa ggttatcgct gtacttaacc agaaaggcgg gtcaggcaag acgaccatcg
     9661 caacccatct agcccgcgcc ctgcaactcg ccggggccga tgttctgtta gtcgattccg
     9721 atccccaggg cagtgcccgc gattgggcgg ccgtgcggga agatcaaccg ctaaccgttg
     9781 tcggcatcga ccgcccgacg attgaccgcg acgtgaaggc catcggccgg cgcgacttcg
     9841 tagtgatcga cggagcgccc caggcggcgg acttggctgt gtccgcgatc aaggcagccg
     9901 acttcgtgct gattccggtg cagccaagcc cttacgacat atgggccacc gccgacctgg
     9961 tggagctggt taagcagcgc attgaggtca cggatggaag gctacaagcg gcctttgtcg
    10021 tgtcgcgggc gatcaaaggc acgcgcatcg gcggtgaggt tgccgaggcg ctggccgggt
    10081 acgagctgcc cattcttgag tcccgtatca cgcagcgcgt gagctaccca ggcactgccg
    10141 ccgccggcac aaccgttctt gaatcagaac ccgagggcga cgctgcccgc gaggtccagg
    10201 cgctggccgc tgaaattaaa tcaaaactca tttgagttaa tgaggtaaag agaaaatgag
    10261 caaaagcaca aacacgctaa gtgccggccg tccgagcgca cgcagcagca aggctgcaac
    10321 gttggccagc ctggcagaca cgccagccat gaagcgggtc aactttcagt tgccggcgga
    10381 ggatcacacc aagctgaaga tgtacgcggt acgccaaggc aagaccatta ccgagctgct
    10441 atctgaatac atcgcgcagc taccagagta aatgagcaaa tgaataaatg agtagatgaa
    10501 ttttagcggc taaaggaggc ggcatggaaa atcaagaaca accaggcacc gacgccgtgg
    10561 aatgccccat gtgtggagga acgggcggtt ggccaggcgt aagcggctgg gttgtctgcc
    10621 ggccctgcaa tggcactgga acccccaagc ccgaggaatc ggcgtgacgg tcgcaaacca
    10681 tccggcccgg tacaaatcgg cgcggcgctg ggtgatgacc tggtggagaa gttgaaggcc
    10741 gcgcaggccg cccagcggca acgcatcgag gcagaagcac gccccggtga atcgtggcaa
    10801 gcggccgctg atcgaatccg caaagaatcc cggcaaccgc cggcagccgg tgcgccgtcg
    10861 attaggaagc cgcccaaggg cgacgagcaa ccagattttt tcgttccgat gctctatgac
    10921 gtgggcaccc gcgatagtcg cagcatcatg gacgtggccg ttttccgtct gtcgaagcgt
    10981 gaccgacgag ctggcgaggt gatccgctac gagcttccag acgggcacgt agaggtttcc
    11041 gcagggccgg ccggcatggc cagtgtgtgg gattacgacc tggtactgat ggcggtttcc
    11101 catctaaccg aatccatgaa ccgataccgg gaagggaagg gagacaagcc cggccgcgtg
    11161 ttccgtccac acgttgcgga cgtactcaag ttctgccggc gagccgatgg cggaaagcag
    11221 aaagacgacc tggtagaaac ctgcattcgg ttaaacacca cgcacgttgc catgcagcgt
    11281 acgaagaagg ccaagaacgg ccgcctggtg acggtatccg agggtgaagc cttgattagc
    11341 cgctacaaga tcgtaaagag cgaaaccggg cggccggagt acatcgagat cgagctagct
    11401 gattggatgt accgcgagat cacagaaggc aagaacccgg acgtgctgac ggttcacccc
    11461 gattactttt tgatcgatcc cggcatcggc cgttttctct accgcctggc acgccgcgcc
    11521 gcaggcaagg cagaagccag atggttgttc aagacgatct acgaacgcag tggcagcgcc
    11581 ggagagttca agaagttctg tttcaccgtg cgcaagctga tcgggtcaaa tgacctgccg
    11641 gagtacgatt tgaaggagga ggcggggcag gctggcccga tcctagtcat gcgctaccgc
    11701 aacctgatcg agggcgaagc atccgccggt tcctaatgta cggagcagat gctagggcaa
    11761 attgccctag caggggaaaa aggtcgaaaa ggcctctttc ctgtggatag cacgtacatt
    11821 gggaacccaa agccgtacat tgggaaccgg aacccgtaca ttgggaaccc aaagccgtac
    11881 attgggaacc ggtcacacat gtaagtgact gatataaaag agaaaaaagg cgatttttcc
    11941 gcctaaaact ctttaaaact tattaaaact cttaaaaccc gcctggcctg tgcataactg
    12001 tctggccagc gcacagccga agagctgcaa aaagcgccta cccttcggtc gctgcgctcc
    12061 ctacgccccg ccgcttcgcg tcggcctatc gcggccgctg gccgctcaaa aatggctggc
    12121 ctacggccag gcaatctacc agggcgcgga caagccgcgc cgtcgccact cgaccgccgg
    12181 cgcccacatc aaggcaccct gcctcgcgcg tttcggtgat gacggtgaaa acctctgaca
    12241 catgcagctc ccggaaacgg tcacagcttg tctgtaagcg gatgccggga gcagacaagc
    12301 ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc gcagccatga cccagtcacg
    12361 tagcgatagc ggagtgtata ctggcttaac tatgcggcat cagagcagat tgtactgaga
    12421 gtgcaccata tgcggtgtga aataccgcac agatgcgtaa ggagaaaata ccgcatcagg
    12481 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg
    12541 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga
    12601 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
    12661 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
    12721 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
    12781 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
    12841 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
    12901 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
    12961 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
    13021 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
    13081 tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca
    13141 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
    13201 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
    13261 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
    13321 ttggtcatgc attctaggta ctaaaacaat tcatccagta aaatataata ttttattttc
    13381 tcccaatcag gcttgatccc cagtaagtca aaaaatagct cgacatactg ttcttccccg
    13441 atatcctccc tgatcgaccg gacgcagaag gcaatgtcat accacttgtc cgccctgccg
    13501 cttctcccaa gatcaataaa gccacttact ttgccatctt tcacaaagat gttgctgtct
    13561 cccaggtcgc cgtgggaaaa gacaagttcc tcttcgggct tttccgtctt taaaaaatca
    13621 tacagctcgc gcggatcttt aaatggagtg tcttcttccc agttttcgca atccacatcg
    13681 gccagatcgt tattcagtaa gtaatccaat tcggctaagc ggctgtctaa gctattcgta
    13741 tagggacaat ccgatatgtc gatggagtga aagagcctga tgcactccgc atacagctcg
    13801 ataatctttt cagggctttg ttcatcttca tactcttccg agcaaaggac gccatcggcc
    13861 tcactcatga gcagattgct ccagccatca tgccgttcaa agtgcaggac ctttggaaca
    13921 ggcagctttc cttccagcca tagcatcatg tccttttccc gttccacatc ataggtggtc
    13981 cctttatacc ggctgtccgt catttttaaa tataggtttt cattttctcc caccagctta
    14041 tataccttag caggagacat tccttccgta tcttttacgc agcggtattt ttcgatcagt
    14101 tttttcaatt ccggtgatat tctcatttta gccatttatt atttccttcc tcttttctac
    14161 agtatttaaa gataccccaa gaagctaatt ataacaagac gaactccaat tcactgttcc
    14221 ttgcattcta aaaccttaaa taccagaaaa cagctttttc aaagttgttt tcaaagttgg
    14281 cgtataacat agtatcgacg gagccgattt tgaaaccgcg gtgatcacag gcagcaacgc
    14341 tctgtcatcg ttacaatcaa catgctaccc tccgcgagat catccgtgtt tcaaacccgg
    14401 cagcttagtt gccgttcttc cgaatagcat cggtaacatg agcaaagtct gccgccttac
    14461 aacggctctc ccgctgacgc cgtcccggac tgatgggctg cctgtatcga gtggtgattt
    14521 tgtgccgagc tgccggtcgg ggagctgttg gctggctggt ggcaggatat attgtggtgt
    14581 aaacaaattg acgcttagac aacttaataa cacattgcgg acgtttttaa tgtactgaat
    14641 taacgccgaa ttaattcggg ggatctggat tttagtactg gattttggtt ttaggaatta
    14701 gaaattttat tgatagaagt attttacaaa tacaaataca tactaagggt ttcttatatg
    14761 ctcaacacat gagcgaaacc ctataggaac cctaattccc ttatctggga actactcaca
    14821 cattattatg gagaaaatag agcttgtcga tcgttaatta atcacctcca agatcccgga
    14881 cgagtgctgg ggcgtcggtt tccactatcg gcgagtactt ctacacagcc atcggtccag
    14941 acggccgcgc ttctgcgggc gatttgtgta cgcccgacag tcccggctcc ggatcggacg
    15001 attgcgtcgc atcgaccctg cgcccaagct gcatcatcga aattgccgtc aaccaagctc
    15061 tgatagagtt ggtcaagacc aatgcggagc atatacgccc ggagccgcgg cgatcctgca
    15121 agctccggat gcctccgctc gaagtagcgc gtctgctgct ccatacaagc caaccacggc
    15181 ctccagaaga agatgttggc gacctcgtat tgggaatccc cgaacatcgc ctcgctccag
    15241 tcaatgaccg ctgttatgcg gccattgtcc gtcaggacat tgttggagcc gaaatccgcg
    15301 tgcacgaggt gccggacttc ggggcagtcc tcggcccaaa gcatcagctc atcgagagcc
    15361 tgcgcgacgg acgcactgac ggtgtcgtcc atcacagttt gccagtgata cacatgggga
    15421 tcagcaatcg cgcatatgaa atcacgccat gtagtgtatt gaccgattcc ttgcggtccg
    15481 aatgggccga acccgctcgt ctggctaaga tcggccgcag cgatcgcatc catggcctcc
    15541 gcgaccggct gcagaacagc gggcagttcg gtttcaggca ggtcttgcaa cgtgacaccc
    15601 tgtgcacggc gggagatgca ataggtcagg ctctcgctga attccccaat gtcaagcact
    15661 tccggaatcg ggagcgcggc cgatgcaaag tgccgataaa cataacgatc tttgtagaaa
    15721 ccatcggcgc agctatttac ccgcaggaca tatccacgcc ctcctacatc gaagctgaaa
    15781 gcacgagatt cttcgccctc cgagagctgc atcaggtcgg acacgctgtc gaacttttcg
    15841 atcagaaact tctcgacaga cgtcgcggtg agttcaggct ttttcatcct aggagagata
    15901 gatttgtaga gagagactgg tgatttcagc gtgtcctctc caaatgaaat gaacttcctt
    15961 atatagagga aggtcttgcg aaggatagtg ggattgtgcg tcatccctta cgtcagtgga
    16021 gatatcacat caatccactt gctttgaaga cgtggttgga acgtcttctt tttccacgat
    16081 gctcctcgtg ggtgggggtc catctttggg accactgtcg gcagaggcat cttgaacgat
    16141 agcctttcct ttatcgcaat gatggcattt gtaggtgcca ccttcctttt ctactgtcct
    16201 tttgatgaag tgacagatag ctgggcaatg gaatccgagg aggtttcccg atattaccct
    16261 ttgttgaaaa gtctcaatag ccctttggtc ttctgagact gtatctttga tattcttgga
    16321 gtagacgaga gtgtcgtgct ccaccatgtt atcacatcaa tccacttgct ttgaagacgt
    16381 ggttggaacg tcttcttttt ccacgatgct cctcgtgggt gggggtccat ctttgggacc
    16441 actgtcggca gaggcatctt gaacgatagc ctttccttta tcgcaatgat ggcatttgta
    16501 ggtgccacct tccttttcta ctgtcctttt gatgaagtga cagatagctg ggcaatggaa
    16561 tccgaggagg tttcccgata ttaccctttg ttgaaaagtc tcaatagccc tttggtcttc
    16621 tgagactgta tctttgatat tcttggagta gacgagagtg tcgtgctcca ccatg
//
