LOCUS       sequence-26630-e         778 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 778)
  AUTHORS   Funakoshi M, Hochstrasser M
  TITLE     Small epitope-linker modules for PCR-based C-terminal tagging in 
            Saccharomyces cerevisiae.
  JOURNAL   Yeast. 2009 Feb 25. 26(3):185-192.
  PUBMED    19243080
REFERENCE   2  (bases 1 to 778)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast. 2009
            Feb 25. 26(3):185-192."
COMMENT     Sequence Label: pFA6a-6xGLY-V5-HIS3MX6
FEATURES             Location/Qualifiers
     source          1..778
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(65..262)
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     CDS             complement(556..570)
                     /codon_start=1
                     /product="tag for protein cleavage using Ni2+ ion (Dang et 
                     al., 2019)"
                     /label=SNAC tag
                     /translation="GSHHW"
     gap             714..742
                     /estimated_length=29
ORIGIN
        1 ctgatatcat cgatgaattc gagctcgttt aaactggatg gcggcgttag tatcgaatcg
       61 acagcagtat agcgaccagc attcacatac gattgacgca tgatattact ttctgcgcac
      121 ttaacttcgc atctgggcag atgatgtcga ggcgaaaaaa aatataaatc acgctaacat
      181 ttgattaaaa tagaacaact acaatataaa aaaactatac aaatgacaag ttcttgaaaa
      241 caagaatctt tttattgtca gtacttcaca tcaaaacacc tttggttgag ggaacgtcat
      301 tggtgccatt gctagaaata gcttctctta tggcaacagc caaagccttg aacgcactct
      361 cacttctgtg gtgatcgttg aaacctctca gacaatcaac atgcaaagta attctggccg
      421 cctccgcgaa actttccaaa aagtgtggaa tcatttcagt ggataaatca ccaatcatct
      481 ctctcttcaa tccaaggtcg attacagcaa atggtctact agataaatcg actacggcac
      541 gtgatagcgc ctcatccaat ggtgcgaacc cagtaccgaa tctttttaca ccacggacag
      601 cacccattgc ttctttgaac gcttgcccta ntgcgatacc gcaatcttcg gtagtatggt
      661 gatcgtcaat gtgcaggtca cnnatacatt caacaataag agaccaacca gagnnnnnnn
      721 nnnnnnnnnn nnnnnnnnnn nncaaaancc caacncctgn gtgaatatcg atganctg
//
