LOCUS       sequence-266301-        6449 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  ced-4S cDNA cloned into pXHA backbone, used for production of 
            antibodies.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6449)
  AUTHORS   Chen F, Hersh BM, Conradt B, Zhou Z, Riemer D, Gruenbaum Y, Horvitz 
            HR
  TITLE     Translocation of C. elegans CED-4 to nuclear membranes during 
            programmed cell death.
  JOURNAL   Science. 2000 Feb 25;287(5457):1485-9. doi: 
            10.1126/science.287.5457.1485.
  PUBMED    10688797
REFERENCE   2  (bases 1 to 6449)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.287.5457"; journalName: "Science"; date: 
            "2000-02-25- 25"; volume: "287"; issue: "5457"; pages: "1485-9"
COMMENT     Sequence Label: ced-4S in pXHA
FEATURES             Location/Qualifiers
     source          1..6449
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1..20
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1..19
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             71..93
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             106..132
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             112..138
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
     primer_bind     complement(1979..1997)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      1993..2040
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        complement(join(2406..2411,2412..2428,2429..2434))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     primer_bind     complement(2461..2479)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        2547..2651
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2652..2720,2721..3512)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2870..2889)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3683..4271
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4172..4191
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4425..4442
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    4457..4596
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(4522..4541)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4682..4704
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(4698..4889)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     primer_bind     6347..6366
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 taatacgact cactataggg agaccacaac ggtttccctc tagcctcgag gcctcgaggc
       61 tagaaataat tttgtttaac tttaagaagg agatatacca tggcttaccc atacgatgtt
      121 ccagattacg ctagcttggg tggtcatatg ctctgcgaaa tcgaatgccg cgctttgagc
      181 acggcacaca cgaggctcat ccacgacttt gaaccacgtg acgcattgac ttatttagaa
      241 ggcaaaaaca ttttcacaga agatcattct gaacttatca gtaaaatgtc aactcgcctc
      301 gagaggatcg ccaattttct tcgaatctat cgacgtcaag cttctgaact tggaccactc
      361 atcgactttt tcaactacaa caatcaaagt caccttgctg atttcctcga agactacatc
      421 gattttgcga taaatgagcc agatctactt cgtccagtag tgattgctcc acaattttcc
      481 cgacaaatgc tcgataggaa actattgctt gggaatgttc caaaacaaat gacatgctat
      541 attcgagagt atcacgtgga tcgagtgatc aaaaagctcg acgagatgtg tgatttagac
      601 tccttttttc tgtttctaca cggccgagct ggatccggaa aatcagtaat tgcatcacaa
      661 gctctttcga aatctgacca acttattgga ataaattatg attcaatcgt ttggctcaaa
      721 gatagtggaa cagctccaaa atctacattc gatttattta cggatatttt gctgatgcta
      781 aaaagcgaag acgatcttct caatttccca tcggtggagc atgtcacgtc agttgtactc
      841 aaaaggatga tctgcaacgc actcattgat cgtccaaata ctttattcgt atttgatgac
      901 gtagttcaag aagaaacaat tcgttgggct caggagctac gtcttcgatg tcttgtaact
      961 actcgtgacg tggaaatatc aaatgctgct tctcaaacat gcgaattcat tgaagtgaca
     1021 tcattggaaa tcgatgaatg ttatgatttt ctagaagctt atggaatgcc gatgcctgtt
     1081 ggagaaaaag aagaagatgt gcttaataaa acaatcgaac taagcagtgg aaatccagca
     1141 acgcttatga tgtttttcaa gtcttgtgaa ccgaaaacat ttgaaaaaat ggcacagctt
     1201 aataacaaat tggaaagtcg aggattagtc ggtgttgaat gtatcacccc ttactcgtac
     1261 aagtcactcg caatggctct tcaaagatgt gttgaagttt tgtcagatga ggatcgaagt
     1321 gctcttgctt tcgcagttgt gatgcctcct ggagttgata tacccgtcaa gctatggtca
     1381 tgtgttattc cagttgatat ttgttcaaat gaagaagaac aattggatga tgaagttgcg
     1441 gatcggttga aaagactcag caaacgtgga gctcttctca gtggaaaacg aatgcccgtt
     1501 ttgacattca aaattgatca tattatccat atgttcttga aacacgtcgt tgatgcacaa
     1561 actatcgcca atggaatctc aattctcgag cagcgtcttc ttgaaatagg aaacaataat
     1621 gtatcagtac cggagcgaca tataccatca catttccaaa aattccgtcg ttcatcagcc
     1681 agtgagatgt atccaaaaac tacagaagaa actgtgatcc gtcctgaaga cttcccaaag
     1741 ttcatgcaat tgcaccagaa attctatgac tccctcaaaa attttgcatg ctgttaaaac
     1801 ctatcgtgta caatattgcc tgtatattcc cctcgaaata cgtttatact ttttcgcacg
     1861 agttttctca ttttttcatt tgtacttgtt ttatttctct ccaaaatttc agatccatat
     1921 atagggcccg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc
     1981 gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg
     2041 ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta
     2101 gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga
     2161 cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac
     2221 gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac
     2281 cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag
     2341 cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact
     2401 accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg
     2461 gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt
     2521 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
     2581 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
     2641 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
     2701 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
     2761 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
     2821 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
     2881 cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc
     2941 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
     3001 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
     3061 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
     3121 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
     3181 acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
     3241 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
     3301 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
     3361 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
     3421 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
     3481 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
     3541 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
     3601 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     3661 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     3721 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     3781 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt
     3841 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     3901 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     3961 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     4021 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     4081 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     4141 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     4201 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     4261 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     4321 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
     4381 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
     4441 aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac
     4501 accgcaatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac
     4561 actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct
     4621 gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc
     4681 tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg
     4741 cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg
     4801 tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg
     4861 ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc
     4921 atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat
     4981 gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg
     5041 gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt
     5101 ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct
     5161 gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct
     5221 caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca
     5281 ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg
     5341 atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg
     5401 gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc
     5461 cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg
     5521 cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga
     5581 caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg
     5641 cataaatcgc cgtgacgatc agcggtccaa tgatcgaagt taggctggta agagccgcga
     5701 gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca
     5761 acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc
     5821 ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa
     5881 tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg
     5941 cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc
     6001 ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa
     6061 agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga
     6121 ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta
     6181 ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat
     6241 gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga
     6301 aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga
     6361 tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt
     6421 agaggatcga gatctcgatc ccgcgaaat
//
