LOCUS       sequence-266305-        4450 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Constitutive mammalian overexpression vector for human bone 
            morphogenetic protein 7 cDNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4450)
  AUTHORS   Feichtinger GA, Hacobian A, Hofmann AT, Wassermann K, Zimmermann A, 
            van Griensven M, Redl H
  TITLE     Constitutive and inducible co-expression systems for non-viral 
            osteoinductive gene therapy.
  JOURNAL   Eur Cell Mater. 2014 Feb 19;27:166-84; discussion 184.
  PUBMED    24554273
REFERENCE   2  (bases 1 to 4450)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Eur Cell 
            Mater."; date: "2014-02-19"; volume: "27"; pages: "166-84; 
            discussion 184"
COMMENT     Sequence Label: pVAX1-BMP7
FEATURES             Location/Qualifiers
     source          1..4450
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        36..415
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        416..619
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     570..590
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     664..683
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        664..682
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2223..2240)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    2229..2453
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     CDS             2626..3420
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             2626..3420
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(2680..2699)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     3290..3309
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     rep_origin      3745..4333
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4234..4253
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gctgcttcgc gatgtacggg ccagatatac gcgttgacat tgattattga ctagttatta
       61 atagtaatca attacggggt cattagttca tagcccatat atggagttcc gcgttacata
      121 acttacggta aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat
      181 aatgacgtat gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga
      241 gtatttacgg taaactgccc acttggcagt acatcaagtg tatcatatgc caagtacgcc
      301 ccctattgac gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt
      361 atgggacttt cctacttggc agtacatcta cgtattagtc atcgctatta ccatggtgat
      421 gcggttttgg cagtacatca atgggcgtgg atagcggttt gactcacggg gatttccaag
      481 tctccacccc attgacgtca atgggagttt gttttggcac caaaatcaac gggactttcc
      541 aaaatgtcgt aacaactccg ccccattgac gcaaatgggc ggtaggcgtg tacggtggga
      601 ggtctatata agcagagctc tctggctaac tagagaaccc actgcttact ggcttatcga
      661 aattaatacg actcactata gggagaccca agctggctag cgtttaaact taagcttgcc
      721 accgcgatgc acgtgcgctc actgcgagct gcggcgccgc acagcttcgt ggcgctctgg
      781 gcacccctgt tcctgctgcg ctccgccctg gccgacttca gcctggacaa cgaggtgcac
      841 tcgagcttca tccaccggcg cctccgcagc caggagcggc gggagatgca gcgcgagatc
      901 ctctccattt tgggcttgcc ccaccgcccg cgcccgcacc tccagggcaa gcacaactcg
      961 gcacccatgt tcatgctgga cctgtacaac gccatggcgg tggaggaggg cggcgggccc
     1021 ggcggccagg gcttctccta cccctacaag gccgtcttca gtacccaggg cccccctctg
     1081 gccagcctgc aagatagcca tttcctcacc gacgccgaca tggtcatgag cttcgtcaac
     1141 ctcgtggaac atgacaagga attcttccac ccacgctacc accatcgaga gttccggttt
     1201 gatctttcca agatcccaga aggggaagct gtcacggcag ccgaattccg gatctacaag
     1261 gactacatcc gggaacgctt cgacaatgag acgttccgga tcagcgttta tcaggtgctc
     1321 caggagcact tgggcaggga atcggatctc ttcctgctcg acagccgtac cctctgggcc
     1381 tcggaggagg gctggctggt gtttgacatc acagccacca gcaaccactg ggtggtcaat
     1441 ccgcggcaca acctgggcct gcagctctcg gtggagacgc tggatgggca gagcatcaac
     1501 cccaagttgg cgggcctgat tgggcggcac gggccccaga acaagcagcc cttcatggtg
     1561 gctttcttca aggccacgga ggtccacttc cgcagcatcc ggtccacggg gagcaaacag
     1621 cgcagccaga accgctccaa gacgcccaag aaccaggaag ccctgcggat ggccaacgtg
     1681 gcagagaaca gcagcagcga ccagaggcag gcctgtaaga agcacgagct gtatgtcagc
     1741 ttccgagacc tgggctggca ggactggatc atcgcgcctg aaggctacgc cgcctactac
     1801 tgtgaggggg agtgtgcctt ccctctgaac tcctacatga acgccaccaa ccacgccatc
     1861 gtgcagacgc tggtccactt catcaacccg gaaacggtgc ccaagccctg ctgtgcgccc
     1921 acgcagctca atgccatctc cgtcctctac ttcgatgaca gctccaacgt catcctgaag
     1981 aaatacagaa acatggtggt ccgggcctgt ggctgccact agctcctccg agaattcaga
     2041 ccctttgggg ccaagttttt ctggatcctc cattgctcgc cttggccagg aaccagcaga
     2101 ccaactgcct tttgtgagac cttcccctcc ctatccccaa ctttaaaggt gtgagagtat
     2161 taggaaacat gagcagcagc ggccgctcga gtctagaggg cccgtttaaa cccgctgatc
     2221 agcctcgact gtgccttcta gttgccagcc atctgttgtt tgcccctccc ccgtgccttc
     2281 cttgaccctg gaaggtgcca ctcccactgt cctttcctaa taaaatgagg aaattgcatc
     2341 gcattgtctg agtaggtgtc attctattct ggggggtggg gtggggcagg acagcaaggg
     2401 ggaggattgg gaagacaata gcaggcatgc tggggatgcg gtgggctcta tggcttctac
     2461 tgggcggttt tatggacagc aagcgaaccg gaattgccag ctggggcgcc ctctggtaag
     2521 gttgggaagc cctgcaaagt aaactggatg gctttcttgc cgccaaggat ctgatggcgc
     2581 aggggatcaa gctctgatca agagacagga tgaggatcgt ttcgcatgat tgaacaagat
     2641 ggattgcacg caggttctcc ggccgcttgg gtggagaggc tattcggcta tgactgggca
     2701 caacagacaa tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg
     2761 gttctttttg tcaagaccga cctgtccggt gccctgaatg aactgcaaga cgaggcagcg
     2821 cggctatcgt ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact
     2881 gaagcgggaa gggactggct gctattgggc gaagtgccgg ggcaggatct cctgtcatct
     2941 caccttgctc ctgccgagaa agtatccatc atggctgatg caatgcggcg gctgcatacg
     3001 cttgatccgg ctacctgccc attcgaccac caagcgaaac atcgcatcga gcgagcacgt
     3061 actcggatgg aagccggtct tgtcgatcag gatgatctgg acgaagagca tcaggggctc
     3121 gcgccagccg aactgttcgc caggctcaag gcgagcatgc ccgacggcga ggatctcgtc
     3181 gtgacccatg gcgatgcctg cttgccgaat atcatggtgg aaaatggccg cttttctgga
     3241 ttcatcgact gtggccggct gggtgtggcg gaccgctatc aggacatagc gttggctacc
     3301 cgtgatattg ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt gctttacggt
     3361 atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga
     3421 attattaacg cttacaattt cctgatgcgg tattttctcc ttacgcatct gtgcggtatt
     3481 tcacaccgca tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt
     3541 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca
     3601 ataatagcac gtgctaaaac ttcattttta atttaaaagg atctaggtga agatcctttt
     3661 tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc
     3721 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
     3781 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
     3841 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt
     3901 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
     3961 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
     4021 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
     4081 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
     4141 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
     4201 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
     4261 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
     4321 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc
     4381 ttttgctcac atgttcttgc tgcttcgcga tgtacgggcc agatatacgc gttgacattg
     4441 attattgact
//
