LOCUS       sequence-266306-        6649 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Constitutive mammalian co-expression vector for human bone 
            morphogenetic protein 2 and 7 cDNAs (2 transcriptional units, 
            tandem).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6649)
  AUTHORS   Feichtinger GA, Hacobian A, Hofmann AT, Wassermann K, Zimmermann A, 
            van Griensven M, Redl H
  TITLE     Constitutive and inducible co-expression systems for non-viral 
            osteoinductive gene therapy.
  JOURNAL   Eur Cell Mater. 2014 Feb 19;27:166-84; discussion 184.
  PUBMED    24554273
REFERENCE   2  (bases 1 to 6649)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Eur Cell 
            Mater."; date: "2014-02-19"; volume: "27"; pages: "166-84; 
            discussion 184"
COMMENT     Sequence Label: pVAX1-BMP2/7 +
FEATURES             Location/Qualifiers
     source          1..6649
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        36..415
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        416..619
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     promoter        664..682
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      764..773
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      768..777
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    2042..2266
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     CDS             2439..3233
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             2439..3233
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(2493..2512)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     3103..3122
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     enhancer        3415..3794
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3795..3998
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     promoter        4043..4061
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     polyA_signal    5608..5832
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5944..6532
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6433..6452
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gctgcttcgc gatgtacggg ccagatatac gcgttgacat tgattattga ctagttatta
       61 atagtaatca attacggggt cattagttca tagcccatat atggagttcc gcgttacata
      121 acttacggta aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat
      181 aatgacgtat gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga
      241 gtatttacgg taaactgccc acttggcagt acatcaagtg tatcatatgc caagtacgcc
      301 ccctattgac gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt
      361 atgggacttt cctacttggc agtacatcta cgtattagtc atcgctatta ccatggtgat
      421 gcggttttgg cagtacatca atgggcgtgg atagcggttt gactcacggg gatttccaag
      481 tctccacccc attgacgtca atgggagttt gttttggcac caaaatcaac gggactttcc
      541 aaaatgtcgt aacaactccg ccccattgac gcaaatgggc ggtaggcgtg tacggtggga
      601 ggtctatata agcagagctc tctggctaac tagagaaccc actgcttact ggcttatcga
      661 aattaatacg actcactata gggagaccca agctggctag cgtttaaact taagcttggt
      721 accgagctcg gatccactag tccagtgtgg tggaattcgg cttgccacca tggtggccgg
      781 gacccgctgt cttctagcgt tgctgcttcc ccaggtcctc ctgggcggcg cggctggcct
      841 cgttccggag ctgggccgca ggaagttcgc ggcggcgtcg tcgggccgcc cctcatccca
      901 gccctctgac gaggtcctga gcgagttcga gctgcggctg ctcagcatgt tcggcctgaa
      961 acagagaccc acccccagca gggacgccgt ggtgcccccc tacatgctag acctgtatcg
     1021 caggcactca ggtcagccgg gctcacccgc cccagaccac cggttggaga gggcagccag
     1081 ccgagccaac actgtgcgca gcttccacca tgaagaatct ttggaagaac taccagaaac
     1141 gagtgggaaa acaacccgga gattcttctt taatttaagt tctatcccca cggaggagtt
     1201 tatcacctca gcagagcttc aggttttccg agaacagatg caagatgctt taggaaacaa
     1261 tagcagtttc catcaccgaa ttaatattta tgaaatcata aaacctgcaa cagccaactc
     1321 gaaattcccc gtgaccagac ttttggacac caggttggtg aatcagaatg caagcaggtg
     1381 ggaaagtctt gatgtcaccc ccgctgtgat gcggtggact gcacagggac acgccaacca
     1441 tggattcgtg gtggaagtgg cccacttgga ggagaaacaa ggtgtctcca agagacatgt
     1501 taggataagc aggtctttgc accaagatga acacagctgg tcacagataa ggccattgct
     1561 agtaactttt ggccatgatg gaaaagggca tcctctccac aaaagagaaa aacgtcaagc
     1621 caaacacaaa cagcggaaac gccttaagtc cagctgtaag agacaccctt tgtacgtgga
     1681 cttcagtgac gtggggtgga atgactggat tgtggctccc ccggggtatc acgcctttta
     1741 ctgccacgga gaatgccctt ttcctctggc tgatcatctg aactccacta atcatgccat
     1801 tgttcagacg ttggtcaact ctgttaactc taagattcct aaggcatgct gtgtcccgac
     1861 agaactcagt gctatctcga tgctgtacct tgacgagaat gaaaaggttg tattaaagaa
     1921 ctatcaggac atggttgtgg agggttgtgg gtgtcgctag aagccgaatt ctgcagatat
     1981 ccagcacagt ggcggccgct cgagtctaga gggcccgttt aaacccgctg atcagcctcg
     2041 actgtgcctt ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc
     2101 ctggaaggtg ccactcccac tgtcctttcc taataaaatg aggaaattgc atcgcattgt
     2161 ctgagtaggt gtcattctat tctggggggt ggggtggggc aggacagcaa gggggaggat
     2221 tgggaagaca atagcaggca tgctggggat gcggtgggct ctatggcttc tactgggcgg
     2281 ttttatggac agcaagcgaa ccggaattgc cagctggggc gccctctggt aaggttggga
     2341 agccctgcaa agtaaactgg atggctttct tgccgccaag gatctgatgg cgcaggggat
     2401 caagctctga tcaagagaca ggatgaggat cgtttcgcat gattgaacaa gatggattgc
     2461 acgcaggttc tccggccgct tgggtggaga ggctattcgg ctatgactgg gcacaacaga
     2521 caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt
     2581 ttgtcaagac cgacctgtcc ggtgccctga atgaactgca agacgaggca gcgcggctat
     2641 cgtggctggc cacgacgggc gttccttgcg cagctgtgct cgacgttgtc actgaagcgg
     2701 gaagggactg gctgctattg ggcgaagtgc cggggcagga tctcctgtca tctcaccttg
     2761 ctcctgccga gaaagtatcc atcatggctg atgcaatgcg gcggctgcat acgcttgatc
     2821 cggctacctg cccattcgac caccaagcga aacatcgcat cgagcgagca cgtactcgga
     2881 tggaagccgg tcttgtcgat caggatgatc tggacgaaga gcatcagggg ctcgcgccag
     2941 ccgaactgtt cgccaggctc aaggcgagca tgcccgacgg cgaggatctc gtcgtgaccc
     3001 atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct ggattcatcg
     3061 actgtggccg gctgggtgtg gcggaccgct atcaggacat agcgttggct acccgtgata
     3121 ttgctgaaga gcttggcggc gaatgggctg accgcttcct cgtgctttac ggtatcgccg
     3181 ctcccgattc gcagcgcatc gccttctatc gccttcttga cgagttcttc tgaattatta
     3241 acgcttacaa tttcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc
     3301 gcatcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa
     3361 atacattcaa atatgtatcc gctcatgtcg atgtacgggc cagatatacg cgttgacatt
     3421 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
     3481 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
     3541 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
     3601 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
     3661 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
     3721 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
     3781 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
     3841 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
     3901 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
     3961 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
     4021 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
     4081 gtttaaactt aagcttgcca ccgcgatgca cgtgcgctca ctgcgagctg cggcgccgca
     4141 cagcttcgtg gcgctctggg cacccctgtt cctgctgcgc tccgccctgg ccgacttcag
     4201 cctggacaac gaggtgcact cgagcttcat ccaccggcgc ctccgcagcc aggagcggcg
     4261 ggagatgcag cgcgagatcc tctccatttt gggcttgccc caccgcccgc gcccgcacct
     4321 ccagggcaag cacaactcgg cacccatgtt catgctggac ctgtacaacg ccatggcggt
     4381 ggaggagggc ggcgggcccg gcggccaggg cttctcctac ccctacaagg ccgtcttcag
     4441 tacccagggc ccccctctgg ccagcctgca agatagccat ttcctcaccg acgccgacat
     4501 ggtcatgagc ttcgtcaacc tcgtggaaca tgacaaggaa ttcttccacc cacgctacca
     4561 ccatcgagag ttccggtttg atctttccaa gatcccagaa ggggaagctg tcacggcagc
     4621 cgaattccgg atctacaagg actacatccg ggaacgcttc gacaatgaga cgttccggat
     4681 cagcgtttat caggtgctcc aggagcactt gggcagggaa tcggatctct tcctgctcga
     4741 cagccgtacc ctctgggcct cggaggaggg ctggctggtg tttgacatca cagccaccag
     4801 caaccactgg gtggtcaatc cgcggcacaa cctgggcctg cagctctcgg tggagacgct
     4861 ggatgggcag agcatcaacc ccaagttggc gggcctgatt gggcggcacg ggccccagaa
     4921 caagcagccc ttcatggtgg ctttcttcaa ggccacggag gtccacttcc gcagcatccg
     4981 gtccacgggg agcaaacagc gcagccagaa ccgctccaag acgcccaaga accaggaagc
     5041 cctgcggatg gccaacgtgg cagagaacag cagcagcgac cagaggcagg cctgtaagaa
     5101 gcacgagctg tatgtcagct tccgagacct gggctggcag gactggatca tcgcgcctga
     5161 aggctacgcc gcctactact gtgaggggga gtgtgccttc cctctgaact cctacatgaa
     5221 cgccaccaac cacgccatcg tgcagacgct ggtccacttc atcaacccgg aaacggtgcc
     5281 caagccctgc tgtgcgccca cgcagctcaa tgccatctcc gtcctctact tcgatgacag
     5341 ctccaacgtc atcctgaaga aatacagaaa catggtggtc cgggcctgtg gctgccacta
     5401 gctcctccga gaattcagac cctttggggc caagtttttc tggatcctcc attgctcgcc
     5461 ttggccagga accagcagac caactgcctt ttgtgagacc ttcccctccc tatccccaac
     5521 tttaaaggtg tgagagtatt aggaaacatg agcagcagcg gccgctcgag tctagagggc
     5581 ccgtttaaac ccgctgatca gcctcgactg tgccttctag ttgccagcca tctgttgttt
     5641 gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc ctttcctaat
     5701 aaaatgagga aattgcatcg cattgtctga gtaggtgtca ttctattctg gggggtgggg
     5761 tggggcagga cagcaagggg gaggattggg aagacaatag caggcatgct ggggatgcgg
     5821 tgggctctat ggcttctact gggcggtttt atggacagca agcgaacaca tgaccaaaat
     5881 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
     5941 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
     6001 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
     6061 cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca
     6121 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
     6181 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
     6241 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
     6301 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
     6361 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
     6421 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
     6481 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
     6541 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttcttgct
     6601 gcttcgcgat gtacgggcca gatatacgcg ttgacattga ttattgact
//
