LOCUS       sequence-266309-        9257 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9257)
  AUTHORS   Rozenblatt-Rosen O, Deo RC, Padi M, Adelmant G, Calderwood MA, 
            Rolland T, Grace M, Dricot A, Askenazi M, Tavares M, Pevzner SJ, 
            Abderazzaq F, Byrdsong D, Carvunis AR, Chen AA, Cheng J, Correll M, 
            Duarte M, Fan C, Feltkamp MC, Ficarro SB, Franchi R, Garg BK, 
            Gulbahce N, Hao T, Holthaus AM, James R, Korkhin A, Litovchick L, 
            Mar JC, Pak TR, Rabello S, Rubio R, Shen Y, Singh S, Spangle JM, 
            Tasan M, Wanamaker S, Webber JT, Roecklein-Canfield J, Johannsen E, 
            Barabasi AL, Beroukhim R, Kieff E, Cusick ME, Hill DE, Munger K, 
            Marto JA, Quackenbush J, Roth FP, DeCaprio JA, Vidal M
  TITLE     Interpreting cancer genomes using systematic host network 
            perturbations by tumour virus proteins.
  JOURNAL   Nature. 2012 Jul 26;487(7408):491-5.
  PUBMED    22810586
REFERENCE   2  (bases 1 to 9257)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature."; 
            date: "2012-07-26"; volume: "487(7408)"; pages: "491-5"
COMMENT     Sequence Label: MSCV-N EBNA3A
FEATURES             Location/Qualifiers
     source          1..9257
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    579..920
                     /label=MESV Psi
                     /note="packaging signal of murine embryonic stem cell 
                     virus"
     CDS             987..1403
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1333..1355
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1371..1387
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     regulatory      1416..1425
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1425..1448
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             1461..1487
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    1503..1527
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(4357..4381)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    4394..4961
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    4411..4963
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4555..4572)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4782..4801
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             4975..5574
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4975..4994)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5471..5491
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             5650..6164
                     /label=3' LTR
                     /note="3' long terminal repeat from murine embryonic stem 
                     cell virus"
     protein_bind    6326..6342
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6350..6356,6357..6374,6375..6380))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6395..6416
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6533..6550)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6704..7292)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6784..6803)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7463..8254,8255..8323))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8086..8105
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8324..8428)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8496..8514
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(8552..8574)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     8674..8693
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8887..8909
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     9037..9056
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     LTR             join(9256..9257,1..515)
                     /label=5' LTR
                     /note="5' long terminal repeat from murine embryonic stem 
                     cell virus"
ORIGIN
        1 tgaaagaccc cacctgtagg tttggcaagc tagcttaagt aacgccattt tgcaaggcat
       61 ggaaaataca taactgagaa tagagaagtt cagatcaagg ttaggaacag agagacagca
      121 gaatatgggc caaacaggat atctgtggta agcagttcct gccccggctc agggccaaga
      181 acagatggtc cccagatgcg gtcccgccct cagcagtttc tagagaacca tcagatgttt
      241 ccagggtgcc ccaaggacct gaaatgaccc tgtgccttat ttgaactaac caatcagttc
      301 gcttctcgct tctgttcgcg cgcttctgct ccccgagctc aataaaagag cccacaaccc
      361 ctcactcggc gcgccagtcc tccgatagac tgcgtcgccc gggtacccgt attcccaata
      421 aagcctcttg ctgtttgcat ccgaatcgtg gactcgctga tccttgggag ggtctcctca
      481 gattgattga ctgcccacct cgggggtctt tcatttggag gttccaccga gatttggaga
      541 cccctgccca gggaccaccg acccccccgc cgggaggtaa gctggccagc ggtcgtttcg
      601 tgtctgtctc tgtctttgtg cgtgtttgtg ccggcatcta atgtttgcgc ctgcgtctgt
      661 actagttagc taactagctc tgtatctggc ggacccgtgg tggaactgac gagttctgaa
      721 cacccggccg caaccctggg agacgtccca gggactttgg gggccgtttt tgtggcccga
      781 cctgaggaag ggagtcgatg tggaatccga ccccgtcagg atatgtggtt ctggtaggag
      841 acgagaacct aaaacagttc ccgcctccgt ctgaattttt gctttcggtt tggaaccgaa
      901 gccgcgcgtc ttgtctgctg cagcgctgca gcatcgttct gtgttgtctc tgtctgactg
      961 tgtttctgta tttgtctgaa aattagggcc agactgttac cactccctta agtttgacct
     1021 taggtcactg gaaagatgtc gagcggatcg ctcacaacca gtcggtagat gtcaagaaga
     1081 gacgttgggt taccttctgc tctgcagaat ggccaacctt taacgtcgga tggccgcgag
     1141 acggcacctt taaccgagac ctcatcaccc aggttaagat caaggtcttt tcacctggcc
     1201 cgcatggaca cccagaccag gtcccctaca tcgtgacctg ggaagccttg gcttttgacc
     1261 cccctccctg ggtcaagccc tttgtacacc ctaagcctcc gcctcctctt cctccatccg
     1321 ccccgtctct cccccttgaa cctcctcgtt cgaccccgcc tcgatcctcc ctttatccag
     1381 ccctcactcc ttctctaggc gccggaatta gatctgccac catggactac aaggatgacg
     1441 atgacaagct cgatggagga tacccatacg atgttccaga ttacgctgct cgaggttatc
     1501 aaacaagttt gtacaaaaaa gttggcatgg acaaggacag gccgggtccc ccggccctgg
     1561 atgacaacat ggaagaagaa gtcccatcta cctcggttgt gcaggaacag gtatcggcgg
     1621 gagattggga aaatgtcctc atagagttat cagatagcag ctcagaaaag gaagcagaag
     1681 atgcccacct ggagccggcc cagaagggta cgaagagaaa gcgggtcgat catgatgccg
     1741 gtgggtcagc tccagcacga cccatgctcc caccccagcc ggatctccct gggcgagaag
     1801 ccattctccg caggtttcca ctagatctaa gaacacttct tcaagcgatt ggagccgcgg
     1861 ctacgcgcat cgacacacga gccatagacc agtttttcgg atcccagatt tcaaataccg
     1921 agatgtacat aatgtatgcc atggccattc gacaggccat tagagatcgt cggagaaatc
     1981 cagcttctcg tagagatcag gccaaatgga gactgcaaac cctggccgcc ggatggccta
     2041 tgggttacca ggcatacagc agctggatgt acagctacac cgatcaccag acgactccca
     2101 cattcgtaca tctccaggcg acacttgggt gcacaggtgg ccgtaggtgt cacgtgacct
     2161 tttccgccgg cacctttaag ctgccgcgat gtacccccgg ggatcgccag tggttgtatg
     2221 ttcagagctc cgtgggtaac attgtacaga gctgtaatcc ccgctacagt attttctttg
     2281 actatatggc tatacacagg agcctcacga aaatctggga ggaagtttta acacctgacc
     2341 agcgtgtttc atttatggaa ttcctaggat ttttgcagag aacggatttg tcctatatca
     2401 agagctttgt cagcgatgcc ctgggcacca ctagtatcca aacaccgtgg atcgatgaca
     2461 atcctagcac ggagacggca caggcttgga atgccggctt tctccggggt cgtgcgtatg
     2521 ggatagactt gcttagaact gaaggggaac atgtcgaagg tgctaccggt gaaacgcgag
     2581 aagaaagtga ggacacggag agcgatggag atgatgaaga tcttccttgt atagtgtcca
     2641 gaggtggacc taaggtcaaa cgacccccta tatttataag acgtctgcac aggttgctgc
     2701 tgatgagagc gggcaaacga acagaacagg gcaaggaggt actggaaaag gcccgtggga
     2761 gcacttatgg cacacctagg ccgcctgttc cgaaaccaag accagaggtc ccacaaagcg
     2821 acgagacagc taccagtcac gggtcggcgc aagtcccaga acccccaacc attcacctag
     2881 cagctcaggg aatggcatac ccattacatg aacaacacgg catggccccg tgtccggtag
     2941 cacaggcccc acctacgccc ttgccccctg tatctccagg ggatcaactc ccaggtgttt
     3001 ttagcgacgg gcgagtggcg tgtgcaccag tacccgcccc ggctgggcct attgtccggc
     3061 cctgggagcc atccctgaca caggctgcgg ggcaggcctt tgcacccgtt agaccacaac
     3121 acatgccagt agaacccgtc cctgtcccga cagtggcact tgagcgacca gtttacccca
     3181 agccagttcg tccggcacct cctaagattg ctatgcaggg ccccggggaa acttctggca
     3241 ttagacgcgc gcgggagcgt tggaggcccg caccttggac gccaaatcca ccccgttctc
     3301 ccagtcagat gtccgtgcgt gaccgtctgg ctcgtttgcg tgctgaggca caggtcaaac
     3361 aggctagtgt tgaggtgcag cccccccagt tgacccaagt atcccctcag caaccaatgg
     3421 aggggccgtt ggtaccagag cagcagatgt tccctggtgc cccctttagc caggttgctg
     3481 atgtggtccg ggcacctggg gtaccggcga tgcagccaca gtactttgac ctccccttaa
     3541 ttcaacccat tagccagggg gcacccgtgg ccccgttgag ggctagtatg ggcccggtac
     3601 ctccggtacc ggcaacacag ccacagtatt ttgacatccc cttaactgaa cccattaacc
     3661 agggggcatc cgcggcccat tttctccctc agcaaccgat ggaggggccg ttggtacctg
     3721 agcagtggat gttcccaggt gccgccctga gccagagtgt taggccaggg gtagcgcagt
     3781 cacaatattt tgacctcccc ttaactcaac ccattaacca tggggcaccc gcagcccatt
     3841 tcctccatca gccaccaatg gaggggccgt gggtacccga gcagtggatg ttccaaggtg
     3901 ccccccctag ccaaggcact gacgtggtcc aacatcagct ggatgctttg gggtatacac
     3961 tccatggtct taaccatccc ggggttcccg tgtctcctgc cgttaaccaa tatcatctca
     4021 gccaggctgc ctttgggtta cctattgatg aggatgagag tggcgagggg tccgatacct
     4081 ccgagccgtg tgaagctctt gatttgtcaa tccatggcag gccctgccct caggcccccg
     4141 agtggcctgt tcaagaggag ggtggccagg atgccaccga ggttcttgat ttgtcaatcc
     4201 atggcaggcc ccgccctcgg acccccgagt ggcctgttca aggggaaggt ggccaaaatg
     4261 tcacaggccc tgaaactaga agggtggtgg tgtcagctgt tgttcacatg tgtcaggatg
     4321 acgagtttcc ggatctacaa gatcctccag atgaggcccc aactttcttg tacaaagtgg
     4381 ttcgataacg aattccgccc ccccccccta acgttactgg ccgaagccgc ttggaataag
     4441 gccggtgtgc gtttgtctat atgttatttt ccaccatatt gccgtctttt ggcaatgtga
     4501 gggcccggaa acctggccct gtcttcttga cgagcattcc taggggtctt tcccctctcg
     4561 ccaaaggaat gcaaggtctg ttgaatgtcg tgaaggaagc agttcctctg gaagcttctt
     4621 gaagacaaac aacgtctgta gcgacccttt gcaggcagcg gaacccccca cctggcgaca
     4681 ggtgcctctg cggccaaaag ccacgtgtat aagatacacc tgcaaaggcg gcacaacccc
     4741 agtgccacgt tgtgagttgg atagttgtgg aaagagtcaa atggctctcc tcaagcgtat
     4801 tcaacaaggg gctgaaggat gcccagaagg taccccattg tatgggatct gatctggggc
     4861 ctcggtgcac atgctttaca tgtgtttagt cgaggttaaa aaaacgtcta ggccccccga
     4921 accacgggga cgtggttttc ctttgaaaaa cacgatgata atatggccac aaccatgacc
     4981 gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg tccccagggc cgtacgcacc
     5041 ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca ccgtcgatcc ggaccgccac
     5101 atcgagcggg tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc
     5161 aaggtgtggg tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc ggagagcgtc
     5221 gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg
     5281 ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg
     5341 tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct gggcagcgcc
     5401 gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt cctggagacc
     5461 tccgcgcccc gcaacctccc cttctacgag cggctcggct tcaccgtcac cgccgacgtc
     5521 gaggtgcccg aaggaccgcg cacctggtgc atgacccgca agcccggtgc ctgatgcatc
     5581 gatgtcgacc tgcagccaag cttatcgata aaataaaaga ttttatttag tctccagaaa
     5641 aaggggggaa tgaaagaccc cacctgtagg tttggcaagc tagcttaagt aacgccattt
     5701 tgcaaggcat ggaaaataca taactgagaa tagagaagtt cagatcaagg ttaggaacag
     5761 agagacagca gaatatgggc caaacaggat atctgtggta agcagttcct gccccggctc
     5821 agggccaaga acagatggtc cccagatgcg gtcccgccct cagcagtttc tagagaacca
     5881 tcagatgttt ccagggtgcc ccaaggacct gaaatgaccc tgtgccttat ttgaactaac
     5941 caatcagttc gcttctcgct tctgttcgcg cgcttctgct ccccgagctc aataaaagag
     6001 cccacaaccc ctcactcggc gcgccagtcc tccgatagac tgcgtcgccc gggtacccgt
     6061 gtatccaata aaccctcttg cagttgcatc cgacttgtgg tctcgctgtt ccttgggagg
     6121 gtctcctctg agtgattgac tacccgtcag cgggggtctt tcatgggtaa cagtttcttg
     6181 aagttggaga acaacattct gagggtagga gtcgaatatt aagtaatcct gactcaatta
     6241 gccactgttt tgaatccaca tactccaata ctcctgaaat agttcattat ggacagcgca
     6301 gaaagagctg gggagaattg tgaaattgtt atccgctcac aattccacac aacatacgag
     6361 ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg
     6421 cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa
     6481 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca
     6541 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
     6601 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
     6661 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
     6721 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
     6781 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
     6841 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
     6901 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
     6961 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
     7021 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
     7081 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
     7141 gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
     7201 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
     7261 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
     7321 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
     7381 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
     7441 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga
     7501 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac
     7561 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg
     7621 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
     7681 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt
     7741 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct
     7801 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat
     7861 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta
     7921 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca
     7981 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat
     8041 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac
     8101 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa
     8161 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt
     8221 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg
     8281 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat
     8341 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
     8401 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtct
     8461 aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg aggccctttc
     8521 gtctcgcgcg tttcggtgat gacggtgaaa acctctgaca catgcagctc ccggagacgg
     8581 tcacagcttg tctgtaagcg gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg
     8641 gtgttggcgg gtgtcggggc tggcttaact atgcggcatc agagcagatt gtactgagag
     8701 tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc
     8761 gccattcgcc attcaggctg cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc
     8821 tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg gtaacgccag
     8881 ggttttccca gtcacgacgt tgtaaaacga cggcgcaagg aatggtgcat gcaaggagat
     8941 ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct
     9001 catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc
     9061 agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggcgatt
     9121 agtccaattt gttaaagaca ggatatcagt ggtccaggct ctagttttga ctcaacaata
     9181 tcaccagctg aagcctatag agtacgagcc atagataaaa taaaagattt tatttagtct
     9241 ccagaaaaag gggggaa
//
