LOCUS       sequence-266310-       10798 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10798)
  AUTHORS   Kuhlman TE, Cox EC
  TITLE     Site-specific chromosomal integration of large synthetic constructs.
  JOURNAL   Nucleic Acids Res. 2010 Apr;38(6):e92. Epub 2010 Jan 4.
  PUBMED    20047970
REFERENCE   2  (bases 1 to 10798)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2010-04"; volume: "38(6)"; pages: "e92. Epub 
            2010 Jan 4"
COMMENT     Sequence Label: pTKRED
FEATURES             Location/Qualifiers
     source          1..10798
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1..681
                     /codon_start=1
                     /gene="exo"
                     /product="5' to 3' double-stranded DNA exonuclease in the 
                     lambda Red system"
                     /label=Exo
                     /note="creates 3' single-stranded DNA tails"
                     /translation="MTPDIILQRTGIDVRAVEQGEDAWHKLRLGVITASEVHNVIAKPR
                     SGKKWPDMKMSYFHTLLAEVCTGVAPEVNAKALAWGKQYENDARTLFEFTSGVNVTESP
                     IIYRDESMRTACSPDGLCSDGNGLELKCPFTSRDFMKFRLGGFEAIKSAYMAQVQYSMW
                     VTRKNAWYFANYDPRMKREGLHYVVIERDEKYMASFDEIVPEFIEKMDEALAEIGFVFG
                     EQWR"
     CDS             complement(1005..1796)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAAYWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     RBS             3091..3099
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     promoter        complement(3126..3410)
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     complement(3184..3203)
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
     CDS             3437..4315
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
     rep_origin      5271..5493
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     CDS             5541..6491
                     /codon_start=1
                     /gene="rep101 encoding an A56V mutation"
                     /product="temperature-sensitive version of the RepA protein
                     needed for replication with the pSC101 origin (Armstrong et
                     al., 1984)"
                     /label=Rep101(Ts)
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYVQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLHNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKYDLNGSFSWLTQKQRTTLENILAKYGRI"
     promoter        6599..6676
                     /gene="lacI"
                     /label=lacI promoter
     CDS             6677..7759
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     complement(6696..6715)
                     /label=LacI-R
                     /note="LacI, reverse primer"
     CDS             8320..9381
                     /codon_start=1
                     /gene="recA"
                     /product="DNA repair protein from E. coli"
                     /label=RecA
                     /translation="MAIDENKQKALAAALGQIEKQFGKGSIMRLGEDRSMDVETISTGS
                     LSLDIALGAGGLPMGRIVEIYGPESSGKTTLTLQVIAAAQREGKTCAFIDAEHALDPIY
                     ARKLGVDIDNLLCSQPDTGEQALEICDALARSGAVDVIVVDSVAALTPKAEIEGEIGDS
                     HMGLAARMMSQAMRKLAGNLKQSNTLLIFINQIRMKIGVMFGNPETTTGGNALKFYASV
                     RLDIRRIGAVKEGENVVGSETRVKVVKNKIAAPFKQAEFQILYGEGINFYGELVDLGVK
                     EKLIEKAGAWYSYKGEKIGQGKANATAWLKDNPETAKEIEKKVRELLLSNPNSTPDFSV
                     DDSEGVAETNEDF"
     promoter        join(9507..9512,9513..9530,9531..9537)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    9545..9561
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9550..9572
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     CDS             9595..10011
                     /codon_start=1
                     /gene="gam"
                     /product="inhibitor of the host RecBCD nuclease in the 
                     lambda Red system"
                     /label=Gam
                     /note="protects the double-stranded DNA substrate for 
                     recombination"
                     /translation="MDINTETEIKQKHSLTPFPVFLISPAFRGRYFHSYFRSSAMNAYY
                     IQDRLEAQSWARHYQQLAREEKEAELADDMEKGLPQHLFESLCIDHLQRHGASKKSITR
                     AFDDDVEFQERMAEHIRYMVETIAHHQVDIDSEV"
     CDS             join(10017..10798,1..4)
                     /codon_start=1
                     /gene="bet"
                     /product="single-stranded DNA binding recombinase in the 
                     lambda Red system"
                     /label=Beta
                     /note="mediates pairing of a single-stranded DNA with a 
                     complementary target"
                     /translation="MSTALATLAGKLAERVGMDSVDPQELITTLRQTAFKGDASDAQFI
                     ALLIVANQYGLNPWTKEIYAFPDKQNGIVPVVGVDGWSRIINENQQFDGMDFEQDNESC
                     TCRIYRKDRNHPICVTEWMDECRREPFKTREGREITGPWQSHPKRMLRHKAMIQCARLA
                     FGFAGIYDKDEAERIVENTAYTAERQPERDITPVNDETMQEINTLLIALDKTWDDDLLP
                     LCSQIFRRDIRASSELTQAEAVKALGFLKQKAAEQKVAA"
ORIGIN
        1 atgacaccgg acattatcct gcagcgtacc gggatcgatg tgagagctgt cgaacagggg
       61 gaagatgcgt ggcacaaatt acggctcggc gtcatcaccg cttcagaagt tcacaacgtg
      121 atagcaaaac cccgctccgg aaagaagtgg cctgacatga aaatgtccta cttccacacc
      181 ctgcttgctg aggtttgcac cggtgtggct ccggaagtta acgctaaagc actggcctgg
      241 ggaaaacagt acgagaacga cgccagaacc ctgtttgaat tcacttccgg cgtgaatgtt
      301 actgaatccc cgatcatcta tcgcgacgaa agtatgcgta ccgcctgctc tcccgatggt
      361 ttatgcagtg acggcaacgg ccttgaactg aaatgcccgt ttacctcccg ggatttcatg
      421 aagttccggc tcggtggttt cgaggccata aagtcagctt acatggccca ggtgcagtac
      481 agcatgtggg tgacgcgaaa aaatgcctgg tactttgcca actatgaccc gcgtatgaag
      541 cgtgaaggcc tgcattatgt cgtgattgag cgggatgaaa agtacatggc gagttttgac
      601 gagatcgtgc cggagttcat cgaaaaaatg gacgaggcac tggctgaaat tggttttgta
      661 tttggggagc aatggcgatg acgcatcctc acgataatat ccgggtaggc gcaatcactt
      721 tcgtctctct agggaattca gatctctgca ggagcttagt aaagccctcg ctagatttta
      781 atgcggatgt tgcgattact tcgccaacta ttgcgataac aagaaaaagc cagcctttca
      841 tgatatatct cccaatttgt gtagggctta ttatgcacgc ttaaaaataa taaaagcaga
      901 cttgacctga tagtttggct gtgagcaatt atgtgcttag tgcatctaac gcttgagtta
      961 agccgcgccg cgaagcggcg tcggcttgaa cgaattgtta gacattattt gccgactacc
     1021 ttggtgatct cgcctttcac gtagtggaca aattcttcca actgatctgc gcgcgaggcc
     1081 aagcgatctt cttcttgtcc aagataagcc tgtctagctt caagtatgac gggctgatac
     1141 tgggccggca ggcgctccat tgcccagtag gcagcgacat ccttcggcgc gattttgccg
     1201 gttactgcgc tgtaccaaat gcgggacaac gtaagcacta catttcgctc atcgccagcc
     1261 cagtcgggcg gcgagttcca tagcgttaag gtttcattta gcgcctcaaa tagatcctgt
     1321 tcaggaaccg gatcaaagag ttcctccgcc gctggaccta ccaaggcaac gctatgttct
     1381 cttgcttttg tcagcaagat agccagatca atgtcgatcg tggctggctc gaagatacct
     1441 gcaagaatgt cattgcgctg ccattctcca aattgcagtt cgcgcttagc tggataacgc
     1501 cacggaatga tgtcgtcgtg cacaacaatg gtgacttcta cagcgcggag aatctcgctc
     1561 tctccagggg aagccgaagt ttccaaaagg tcgttgatca aagctcgccg cgttgtttca
     1621 tcaagcctta cggtcaccgt aaccagcaaa tcaatatcac tgtgtggctt caggccgcca
     1681 tccactgcgg agccgtacaa atgtacggcc agcaacgtcg gttcgagatg gcgctcgatg
     1741 acgccaacta cctctgatag ttgagtcgat acttcggcga tcaccgcttc cctcatgatg
     1801 tttaactttg ttttagggcg actgccctgc tgcgtaacat cgttgctgct ccataacatc
     1861 aaacatcgac ccacggcgta acgcgcttgc tgcttggatg cccgaggcat agactgtacc
     1921 ccaaaaaaac agtcataaca agccatgaaa accgccactg cgccgttacc accgctgcgt
     1981 tcggtcaagg ttctggacca gttgcgtgag cgcatacgct acttgcatta cagcttacga
     2041 accgaacagg cttatgtcca ctgggttcgt gccttcatcc gtttccacgg tgtgcgtcac
     2101 ccggcaacct tgggcagcag cgaagtcgag gcatttctgt cctggctggc gaacgagcgc
     2161 aaggtttcgg tctccacgca tcgtcaggca ttggcggcct tgctgttctt ctacggcaag
     2221 gtgctgtgca cggatctgcc ctggcttcag gagatcggaa gacctcggcc gtcgcggcgc
     2281 ttgccggtgg tgctgacccc ggatgaagtg gttcgcatcc tcggttttct ggaaggcgag
     2341 catcgtttgt tcgcccagct tctgtatgga acgggcatgc ttatttcagg aaagtttcgg
     2401 aggagatagt gttcggcagt ttgtacatca tctgcgggat caggtacggt ttgatcaggt
     2461 tgtagaagat caggtaagac atagaatcga tgtagatgat cggtttgttt ttgttgattt
     2521 ttacgtaaca gttcagttgg aatttgttac gcagaccctt aaccaggtat tctacttctt
     2581 cgaaagtgaa agactgggtg ttcagtacga tcgatttgtt ggtagagttt ttgttgtaat
     2641 cccatttacc accatcatcc atgaaccagt atgccagaga catcggggtc aggtagtttt
     2701 caaccaggtt gttcgggatg gtttttttgt tgttaacgat gaacaggtta gccagtttgt
     2761 tgaaagcttg gtgtttgaaa gtctgggcgc cccaggtgat taccaggtta cccaggtggt
     2821 taacacgttc ttttttgtgc ggcggggaca gtacccactg atcgtacagc agacatacgt
     2881 ggtccatgta tgctttgttt ttccactcga actgcataca gtaggtttta ccttcatcac
     2941 gagaacggat gtaagcatca cccaggatca gaccgatacc tgcttcgaac tgttcgatgt
     3001 tcagttcgat cagctgggat ttgtattctt tcagcagttt agagttcgga cccaggttca
     3061 ttacctggtt tttttgatgc attttttata acctccttag agctcgaatt cccaaaaaaa
     3121 cgggtatgga gaaacagtag agagttgcga taaaaagcgt caggtaggat ccgctaatct
     3181 tatggataaa aatgctatgg catagcaaag tgtgacgccg tgcaaataat caatgtggac
     3241 ttttctgccg tgattataga cacttttgtt acgcgttttt gtcatggctt tggtcccgct
     3301 ttgttacaga atgcttttaa taagcggggt taccggtttg gttagcgaga agagccagta
     3361 aaagacgcag tgacggcaat gtctgatgca atatggacaa ttggtttctt ctctgaatgg
     3421 cgggagtatg aaaagtatgg ctgaagcgca aaatgatccc ctgctgccgg gatactcgtt
     3481 taatgcccat ctggtggcgg gtttaacgcc gattgaggcc aacggttatc tcgatttttt
     3541 tatcgaccga ccgctgggaa tgaaaggtta tattctcaat ctcaccattc gcggtcaggg
     3601 ggtggtgaaa aatcagggac gagaatttgt ttgccgaccg ggtgatattt tgctgttccc
     3661 gccaggagag attcatcact acggtcgtca tccggaggct cgcgaatggt atcaccagtg
     3721 ggtttacttt cgtccgcgcg cctactggca tgaatggctt aactggccgt caatatttgc
     3781 caatacgggg ttctttcgcc cggatgaagc gcaccagccg catttcagcg acctgtttgg
     3841 gcaaatcatt aacgccgggc aaggggaagg gcgctattcg gagctgctgg cgataaatct
     3901 gcttgagcaa ttgttactgc ggcgcatgga agcgattaac gagtcgctcc atccaccgat
     3961 ggataatcgg gtacgcgagg cttgtcagta catcagcgat cacctggcag acagcaattt
     4021 tgatatcgcc agcgtcgcac agcatgtttg cttgtcgccg tcgcgtctgt cacatctttt
     4081 ccgccagcag ttagggatta gcgtcttaag ctggcgcgag gaccaacgta tcagccaggc
     4141 gaagctgctt ttgagcacca cccggatgcc tatcgccacc gtcggtcgca atgttggttt
     4201 tgacgatcaa ctctatttct cgcgggtatt taaaaaatgc accggggcca gcccgagcga
     4261 gttccgtgcc ggttgtgaag aaaaagtgaa tgatgtagcc gtcaagttgt cataagcatg
     4321 cggatcagtg agggtttgca actgcgggtc aaggatctgg atttcgatca cggcacgatc
     4381 atcgtgcggg agggcaaggg ctccaaggat cgggccttga tgttacccga gagcttggca
     4441 cccagcctgc gcgagcagcg gctggctggt ttattgctga taaatctgga gccggtgagc
     4501 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
     4561 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
     4621 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
     4681 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
     4741 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgttg
     4801 atgataccgc tgccttactg ggtgcattag ccagtctgaa tgacctgtca cgggataatc
     4861 cgaagtggtc agactggaaa atcagagggc aggaactgct gaacagcaaa aagtcagata
     4921 gcaccacata gcagacccgc cataaaacgc cctgagaagc ccgtgacggg cttttcttgt
     4981 attatgggta gtttccttgc atgaatccat aaaaggcgcc tgtagtgcca tttaccccca
     5041 ttcactgcca gagccgtgag cgcagcgaac tgaatgtcac gaaaaagaca gcgactcagg
     5101 tgcctgatgg tcggagacaa aaggaatatt cagcgatttg cccgattgcg gccgcaaccg
     5161 agcttgcgag ggtgctactt aagcctttag ggttttaagg tctgttttgt agaggagcaa
     5221 acagcgtttg cgacatcctt ttgtaatact gcggaactga ctaaagtagt gagttataca
     5281 cagggctggg atctattctt tttatctttt tttattcttt ctttattcta taaattataa
     5341 ccacttgaat ataaacaaaa aaaacacaca aaggtctagc ggaatttaca gagggtctag
     5401 cagaatttac aagttttcca gcaaaggtct agcagaattt acagataccc acaactcaaa
     5461 ggaaaaggac tagtaattat cattgactag cccatctcaa ttggtatagt gattaaaatc
     5521 acctagacca attgagatgt atgtctgaat tagttgtttt caaagcaaat gaactagcga
     5581 ttagtcgcta tgacttaacg gagcatgaaa ccaagctaat tttatgctgt gtggcactac
     5641 tcaaccccac gattgaaaac cctacaagga aagaacggac ggtatcgttc acttataacc
     5701 aatacgttca gatgatgaac atcagtaggg aaaatgctta tggtgtatta gctaaagcaa
     5761 ccagagagct gatgacgaga actgtggaaa tcaggaatcc tttggttaaa ggctttgaga
     5821 ttttccagtg gacaaactat gccaagttct caagcgaaaa attagaatta gtttttagtg
     5881 aagagatatt gccttatctt ttccagttaa aaaaattcat aaaatataat ctggaacatg
     5941 ttaagtcttt tgaaaacaaa tactctatga ggatttatga gtggttatta aaagaactaa
     6001 cacaaaagaa aactcacaag gcaaatatag agattagcct tgatgaattt aagttcatgt
     6061 taatgcttga aaataactac catgagttta aaaggcttaa ccaatgggtt ttgaaaccaa
     6121 taagtaaaga tttaaacact tacagcaata tgaaattggt ggttgataag cgaggccgcc
     6181 cgactgatac gttgattttc caagttgaac tagatagaca aatggatctc gtaaccgaac
     6241 ttgagaacaa ccagataaaa atgaatggtg acaaaatacc aacaaccatt acatcagatt
     6301 cctacctaca taacggacta agaaaaacac tacacgatgc tttaactgca aaaattcagc
     6361 tcaccagttt tgaggcaaaa tttttgagtg acatgcaaag taagtatgat ctcaatggtt
     6421 cgttctcatg gctcacgcaa aaacaacgaa ccacactaga gaacatactg gctaaatacg
     6481 gaaggatctg aggttcttat ggctcttgta tctatcagtg aagcatcaag actaacaaac
     6541 aaaagtagaa caactgttca ccgttacata tcaaagggaa aactgtccat acccatgggc
     6601 tagcatcgaa tggcgcaaaa cctttcgcgg tatggcatga tagcgcccgg aagagagtca
     6661 attcagggtg gtgaatgtga aaccagtaac gttatacgat gtcgcagagt atgccggtgt
     6721 ctcttatcag accgtttccc gcgtggtgaa ccaggccagc cacgtttctg cgaaaacgcg
     6781 ggaaaaagtg gaagcggcga tggcggagct gaattacatt cccaaccgcg tggcacaaca
     6841 actggcgggc aaacagtcgt tgctgattgg cgttgccacc tccagtctgg ccctgcacgc
     6901 gccgtcgcaa attgtcgcgg cgattaaatc tcgcgccgat caactgggtg ccagcgtggt
     6961 ggtgtcgatg gtagaacgaa gcggcgtcga agcctgtaaa gcggcggtgc acaatcttct
     7021 cgcgcaacgc gtcagtgggc tgatcattaa ctatccgctg gatgaccagg atgccattgc
     7081 tgtggaagct gcctgcacta atgttccggc gttatttctt gatgtctctg accagacacc
     7141 catcaacagt attattttct cccatgaaga cggtacgcga ctgggcgtgg agcatctggt
     7201 cgcattgggt caccagcaaa tcgcgctgtt agcgggccca ttaagttctg tctcggcgcg
     7261 tctgcgtctg gctggctggc ataaatatct cactcgcaat caaattcagc cgatagcgga
     7321 acgggaaggc gactggagtg ccatgtccgg ttttcaacaa accatgcaaa tgctgaatga
     7381 gggcatcgtt cccactgcga tgctggttgc caacgatcag atggcgctgg gcgcaatgcg
     7441 cgccattacc gagtccgggc tgcgcgttgg tgcggatatc tcggtagtgg gatacgacga
     7501 taccgaagac agctcatgtt atatcccgcc gttaaccacc atcaaacagg attttcgcct
     7561 gctggggcaa accagcgtgg accgcttgct gcaactctct cagggccagg cggtgaaggg
     7621 caatcagctg ttgcccgtct cactggtgaa aagaaaaacc accctggcgc ccaatacgca
     7681 aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg
     7741 actggaaagc gggcagtgag ctagctcact gcccgctttc cagtcgggaa accatcatgc
     7801 cataccgcga aaggttttgc gccattcgat gctagctgat cagccagtgc caagcttggc
     7861 attgcttatc aatttgttgc aacgaacagg tcactatcag tcaaaataaa atcattattt
     7921 gatttcaatt ttgtcccact ccctgcctct gtcatcacga tactgtgatg ccatggtgtc
     7981 cgacttatgc ccgagaagat gttgagcaaa cttatcgctt atctgcttct catagagtct
     8041 tgcagacaaa ctgcgcaact cgtgaaaggt aggcggatct agagaagcct gtcggcaccg
     8101 tctggtttgc ttttgccact gcccgcggtg aaggcattac ccggcgggaa tgcttcagcg
     8161 gcgaccgtga tgcggtgcgt cgtcaggcta ctgcgtatgc attgcagacc ttgtggcaac
     8221 aatttctaca aaacacttga tactgtatga gcatacagta taattgcttc aacagaacat
     8281 attgactatc cggtattacc cggcatgaca ggagtaaaaa tggctatcga cgaaaacaaa
     8341 cagaaagcgt tggcggcagc actgggccag attgagaaac aatttggtaa aggctccatc
     8401 atgcgcctgg gtgaagaccg ttccatggat gtggaaacca tctctaccgg ttcgctttca
     8461 ctggatatcg cgcttggggc aggtggtctg ccgatgggcc gtatcgtcga aatctacgga
     8521 ccggaatctt ccggtaaaac cacgctgacg ctgcaggtga tcgccgcagc gcagcgtgaa
     8581 ggtaaaacct gtgcgtttat cgatgctgaa cacgcgctgg acccaatcta cgcacgtaaa
     8641 ctgggcgtcg atatcgacaa cctgctgtgc tcccagccgg acaccggcga gcaggcactg
     8701 gaaatctgtg acgccctggc gcgttctggc gcagtagacg ttatcgtcgt tgactccgtg
     8761 gcggcactga cgccgaaagc ggaaatcgaa ggcgaaatcg gcgactctca catgggcctt
     8821 gcggcacgta tgatgagcca ggcgatgcgt aagctggcgg gtaacctgaa gcagtccaac
     8881 acgctgctga tcttcatcaa ccagatccgt atgaaaattg gtgtgatgtt cggtaacccg
     8941 gaaaccacta ccggtggtaa cgcgctgaaa ttctacgcct ctgttcgtct cgacatccgt
     9001 cgtatcggcg cggtgaaaga gggcgaaaac gtggtgggta gcgaaacccg cgtgaaagtg
     9061 gtgaagaaca aaatcgctgc gccgtttaaa caggctgaat tccagatcct ctacggcgaa
     9121 ggtatcaact tctacggcga actggttgac ctgggcgtaa aagagaagct gatcgagaaa
     9181 gcaggcgcgt ggtacagcta caaaggtgag aagatcggtc agggtaaagc gaatgcgact
     9241 gcctggctga aagataaccc ggaaaccgcg aaagagatcg agaagaaagt acgtgagttg
     9301 ctgctgagca acccgaactc aacgccggat ttctctgtag atgatagcga aggcgtagca
     9361 gaaactaacg aagattttta atcgtcttgt ttgatacaca agggtcgcat ctgcggccct
     9421 tttgcttttt taagttgtaa ggatatgcca tgacagaatc aacatcccgt cgagatccga
     9481 attctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg tataatgtgt
     9541 ggaattgtga gcggataaca atttcacaca ggaaacagtc gacgcttata aaaaatggat
     9601 attaatactg aaactgagat caagcaaaag cattcactaa ccccctttcc tgttttccta
     9661 atcagcccgg catttcgcgg gcgatatttt cacagctatt tcaggagttc agccatgaac
     9721 gcttattaca ttcaggatcg tcttgaggct cagagctggg cgcgtcacta ccagcagctc
     9781 gcccgtgaag agaaagaggc agaactggca gacgacatgg aaaaaggcct gccccagcac
     9841 ctgtttgaat cgctatgcat cgatcatttg caacgccacg gggccagcaa aaaatccatt
     9901 acccgtgcgt ttgatgacga tgttgagttt caggagcgca tggcagaaca catccggtac
     9961 atggttgaaa ccattgctca ccaccaggtt gatattgatt cagaggtata aaacgaatga
    10021 gtactgcact cgcaacgctg gctgggaagc tggctgaacg tgtcggcatg gattctgtcg
    10081 acccacagga actgatcacc actcttcgcc agacggcatt taaaggtgat gccagcgatg
    10141 cgcagttcat cgcattactg atcgttgcca accagtacgg ccttaatccg tggacgaaag
    10201 aaatttacgc ctttcctgat aagcagaatg gcatcgttcc ggtggtgggc gttgatggct
    10261 ggtcccgcat catcaatgaa aaccagcagt ttgatggcat ggactttgag caggacaatg
    10321 aatcctgtac atgccggatt taccgcaagg accgtaatca tccgatctgc gttaccgaat
    10381 ggatggatga atgccgccgc gaaccattca aaactcgcga aggcagagaa atcacggggc
    10441 cgtggcagtc gcatcccaaa cggatgttac gtcataaagc catgattcag tgtgcccgtc
    10501 tggccttcgg atttgctggt atctatgaca aggatgaagc cgagcgcatt gtcgaaaata
    10561 ctgcatacac tgcagaacgt cagccggaac gcgacatcac tccggttaac gatgaaacaa
    10621 tgcaggagat taacactctg ctgatcgccc tggataaaac atgggatgac gacttattgc
    10681 cgctctgttc ccagatattt cgccgcgaca ttcgtgcatc gtcagaactg acacaggccg
    10741 aagcagtaaa agctcttgga ttcctgaaac agaaagccgc agagcagaag gtggcagc
//
