LOCUS       sequence-266342-       10218 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of the barley Mla1 NLR from the Zea mays ubiquitin 
            promotor.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10218)
  AUTHORS   Saur IML, Bauer S, Lu X, Schulze-Lefert P
  TITLE     A cell death assay in barley and wheat protoplasts for 
            identification and validation of matching pathogen AVR effector and 
            plant NLR immune receptors.
  JOURNAL   Plant Methods. 2019 Oct 24;15:118. doi: 10.1186/s13007-019-0502-0. 
            eCollection 2019.
  PUBMED    31666804
REFERENCE   2  (bases 1 to 10218)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Methods."; date: "2019-10-24"; volume: "15:118. doi"; pages: " 
            10.1186/s13007-019-0502-0. eCollection 2019"
COMMENT     Sequence Label: pZmUBQ:Mla1
FEATURES             Location/Qualifiers
     source          1..10218
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1313..1492
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     CDS             1534..2085
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     terminator      2105..2357
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    2451..2475
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     rep_origin      complement(2787..3375)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2867..2886)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3546..4337,4338..4406))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4169..4188
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4407..4498)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      4571..5280
                     /label=oriV
                     /note="incP origin of replication"
     misc_feature    5392..5416
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             complement(join(5709..6422,6423..6425,6426..6428))
                     /codon_start=1
                     /product="enhanced YFP"
                     /label=EYFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(5754..5775)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     6103..6122
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6362..6383
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     protein_bind    6436..6460
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(9374..9398)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(9432..9448)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9433..9449)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
ORIGIN
        1 aatcgatccc gtctgcggaa cggctagagc catcccagga ttccccaaag agaaacactg
       61 gcaagttagc aatcagaacg tgtctgacgt acaggtcgca tccgtgtacg aacgctagca
      121 gcacggatct atcacaaaca cggatctaac acaaacatga acagaagtag aactaccggg
      181 ccctaaccat ggaccggaac gccgatctag agaaggtaga gagggggggg ggaggacgag
      241 cggcgtacct tgaagcggag gtgccgacgg gtggatttgg gggagatctg gttgtgtgtg
      301 tgtgcgctcc gaacaacacg aggttgggga aagagggtgt ggagggggtg tctatttatt
      361 acggcgggcg aggaagggaa agcgaaggag cggtgggaaa ggaatccccc gtagctgccg
      421 gtgccgtgag aggaggagga ggccgcctgc cgtgccggct cacgtctgcc gctccgccac
      481 gcaatttctg gatgccgaca gcggagcaag tccaacggtg gagcggaact ctcgagaggg
      541 gcccagaggc agcgacagag atgccgtgcc gtctgcttcg cttggcccga cgcgacgctg
      601 ctggttcgct ggttggtgtc cgttagactc gtcgacggcg tttaacaggc tggcattatc
      661 tactcgaaac aagaaaaatg tttccttagt ttttttaatt tcttaaaggg tatttgttta
      721 atttttagtc actttatttt attctatttt atatctaaat tattaaataa aaaaactaaa
      781 atagagtttt agttttctta atttagaggc taaaatagaa taaaatagat gtactaaaaa
      841 aattagtcta taaaaaccat taaccctaaa ccctaaatgg atgtactaat aaaatggatg
      901 aagtattata taggtgaagc tatttgcaaa aaaaaaggag aacacatgca cactaaagag
      961 ataaaactgt agagtcctgt tgtcaaagta ctcaattgtc ctttagacca tgtctaactg
     1021 ttcatttata tgattctcta aaacactgat attattgtag tactatagat tatattattc
     1081 gtagagtgaa gtttaaatat atgtataaag atagataaac tgcacttcaa acaagtgtga
     1141 caaaaaaata tgtggtaatt ttttataact tagacatgca atgctcatta aatcggcgcg
     1201 cccaaaccga aggcgggaaa cgacaatctg atcatgagcg gagaattaag ggagtcacgt
     1261 tatgaccccc gccgatgacg cgggacaagc cgttttacgt ttggaactga cagaaccgca
     1321 acgttgaagg agccactgag ccgcgggttt ctggagttta atgagctaag cacatacgtc
     1381 agaaaccatt attgcgcgtt caaaagtcgc ctaaggtcac tatcagctag caaatatttc
     1441 ttgtcaaaaa tgctccactg acgttccata aattcccctc ggtatccaat tagagtctca
     1501 tattcactct caactcgatc gaggggatct accatgagcc cagaacgacg cccggccgac
     1561 atccgccgtg ccaccgaggc ggacatgccg gcggtctgca ccatcgtcaa ccactacatc
     1621 gagacaagca cggtcaactt ccgtaccgag ccgcaggaac cgcaggagtg gacggacgac
     1681 ctcgtccgtc tgcgggagcg ctatccctgg ctcgtcgccg aggtggacgg cgaggtcgcc
     1741 ggcatcgcct acgcgggtcc ctggaaggca cgcaacgcct acgactggac ggccgagtcg
     1801 accgtgtacg tctccccccg ccaccagcgg acgggactgg gctccacgct ctacacccac
     1861 ctgctgaagt ccctggaggc acagggcttc aagagcgtgg tcgctgtcat cgggctgccc
     1921 aacgacccga gcgtgcgcat gcacgaggcg ctcggatatg ccccccgcgg catgctgcgg
     1981 gcggccggct tcaagcacgg gaactggcat gacgtgggtt tctggcagct ggacttcagc
     2041 ctgccggtgc cgccccgtcc ggtcctgccc gtcaccgaaa tctgatgacc cctagagtca
     2101 agcagatcgt tcaaacattt ggcaataaag tttcttaaga ttgaatcctg ttgccggtct
     2161 tgcgatgatt atcatataat ttctgttgaa ttacgttaag catgtaataa ttaacatgta
     2221 atgcatgacg ttatttatga gatgggtttt tatgattaga gtcccgcaat tatacattta
     2281 atacgcgata gaaaacaaaa tatagcgcgc aaactaggat aaattatcgc gcgcggtgtc
     2341 atctatgtta ctagatcgac cggcatgcaa gctgatatct ggtagagacg ttagtattcg
     2401 atcattaaaa acgtccgcaa tgtgttatta agttgtctaa gcgtcaattt gtttacacca
     2461 caatatatcc tgccaccagc cagccaacag ctccccgacc ggcagctcgg cacaaaatca
     2521 ccactcgata caggcagccc atcagtccgg gacggcgtca gcgggagagc cgttgtaagg
     2581 cggcagactt tgctcatgtt accgatgcta ttcggaagaa cggcaactaa gctgccgggt
     2641 ttgaaacacg gatgatctcg cggagggtag catgttgatt gtaacgatga cagagcgttg
     2701 ctgcctgtga tcctctagct agagtccatg tgagcaaaag gccagcaaaa ggccaggaac
     2761 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
     2821 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
     2881 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     2941 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
     3001 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
     3061 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
     3121 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
     3181 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt
     3241 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
     3301 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
     3361 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
     3421 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
     3481 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
     3541 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
     3601 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
     3661 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
     3721 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
     3781 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
     3841 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
     3901 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
     3961 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
     4021 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
     4081 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
     4141 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa
     4201 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
     4261 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
     4321 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
     4381 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
     4441 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
     4501 ggggtagctt tcgggctgat cgcccatccc gttactgccc cgatcccggc aatggcaagg
     4561 actgccagcg ctgccatttt tggggtgagg ccgttcgcgg ccgaggggcg cagcccctgg
     4621 ggggatggga ggcccgcgtt agcgggccgg gagggttcga gaaggggggg cacccccctt
     4681 cggcgtgcgc ggtcacgcgc acagggcgca gccctggtta aaaacaaggt ttataaatat
     4741 tggtttaaaa gcaggttaaa agacaggtta gcggtggccg aaaaacgggc ggaaaccctt
     4801 gcaaatgctg gattttctgc ctgtggacag cccctcaaat gtcaataggt gcgcccctca
     4861 tctgtcagca ctctgcccct caagtgtcaa ggatcgcgcc cctcatctgt cagtagtcgc
     4921 gcccctcaag tgtcaatacc gcagggcact tatccccagg cttgtccaca tcatctgtgg
     4981 gaaactcgcg taaaatcagg cgttttcgcc gatttgcgag gctggccagc tccacgtcgc
     5041 cggccgaaat cgagcctgcc cctcatctgt caacgccgcg ccgggtgagt cggcccctca
     5101 agtgtcaacg tccgcccctc atctgtcagt gagggccaag ttttccgcga ggtatccaca
     5161 acgccggcgg ccggtgtctc gcacacggct tcgacggcgt ttctggcgcg tttgcagggc
     5221 catagacggc cgccagccca gcggcgaggg caaccagccc ggtgagcgtc ggaaaggcgc
     5281 tcggtcttgc cttgctcgtc ggtgatgtac acatacaaat ggacgaacgg ataaaccttt
     5341 tcacgccctt ttaaatatcc gattattcta ataaacgctc ttttctctta ggtttacccg
     5401 ccaatatatc ctgtcaaaca ctgatagttt aaactgaagg cgggaaacga caatctgatc
     5461 cccctccacc gcggtggcgg ccgcccggtc actggatttt ggttttagga attagaaatt
     5521 ttattgatag aagtatttta caaatacaaa tacatactaa gggtttctta tatgctcaac
     5581 acatgagcga aaccctataa gaaccctaat tcccttatct gggaactact cacacattat
     5641 tctggagaaa aatagagaga gatagatttg tagagagaga ctggtgattt ttgcggactc
     5701 tagagctctt acttgtacag ctcgtccatg ccgagagtga tcccggcggc ggtcacgaac
     5761 tccagcagga ccatgtgatc gcgcttctcg ttggggtctt tgctcagggc ggactggtag
     5821 ctcaggtagt ggttgtcggg cagcagcacg gggccgtcgc cgatgggggt gttctgctgg
     5881 tagtggtcgg cgagctgcac gctgccgtcc tcgatgttgt ggcggatctt gaagttcacc
     5941 ttgatgccgt tcttctgctt gtcggccatg atatagacgt tgtggctgtt gtagttgtac
     6001 tccagcttgt gccccaggat gttgccgtcc tccttgaagt cgatgccctt cagctcgatg
     6061 cggttcacca gggtgtcgcc ctcgaacttc acctcggcgc gggtcttgta gttgccgtcg
     6121 tccttgaaga agatggtgcg ctcctggacg tagccttcgg gcatggcgga cttgaagaag
     6181 tcgtgctgct tcatgtggtc ggggtagcgg gcgaagcact gcacgccgta gccgaaggtg
     6241 gtcacgaggg tgggccaggg cacgggcagc ttgccggtgg tgcagatgaa cttcagggtc
     6301 agcttgccgt aggtggcatc gccctcgccc tcgccggaca cgctgaactt gtggccgttt
     6361 acgtcgccgt ccagctcgac caggatgggc accaccccgg tgaacagctc ctcgcccttg
     6421 ctcaccatcc catcaaccac tttgtacaag aaagctgggt cggcgcgccc accctttcag
     6481 ttctcctcct cgtcctcaca caaatcgtca tcatgagcac cttttgctat atgcggcctc
     6541 atctgaatat aaatccgggg atggctggga tgagcttcca gggcacgcct caccgcagcc
     6601 tccgcttcct ttgcctcccc aaccctcgct ccaccacaat acatataaac agagacaaac
     6661 tctcgaaggg agaacaggtt cccctgcagg cccaagtcga agccacggtt accatcctct
     6721 ttcgtcaccc gcacgccaag gctgaaccaa actcttaccg cccttggcaa agctcctggt
     6781 tcaaacaata tctgcgtggc agatccacaa tccaatcgaa agtcaaccgt acagcggaac
     6841 ccatctgcac ggatgactag cagccgttgc gtttggtcgg tgctcgttat tataaagaga
     6901 cgacgaaggc acaacaaccc cccaatgatt tccacgtcat cctgctgcac gtccttcact
     6961 gacgagagga ttaactcgga gaggttcgag agatgggagg ggtctctctt tatccaccct
     7021 cgcagtgcag agagttggct aggcatggat gacacaaatt cacggaaatg tacaggaggc
     7081 acccagcgtt ctcccaagag atccaccagt tcaaaagatg atgtttctct ggaattgcac
     7141 tcaatacgta gactttcgat gtgatgtagg ttgcacagag acttcacgaa atctttatac
     7201 aaatccaaac taccatccct gaagcaaata tcaagcaccc tcagcctttc caggttgcca
     7261 agctcttgtg caataatgtt cacagagacc aagatccccc tcaacacttc tatggatgtc
     7321 agattttgca acacaccaac tggaggaacc accggacacc caaataaatt taggtagatt
     7381 aatcttctga aattacaaac agtggacggc aattccttta gattatgatt gtttccaaga
     7441 tccaacacct ccaaaaactg cagttttcct atctcagcag ggagcttact gatgttggta
     7501 ccttctagac caaggtacct taggtgagtt aaatgtccaa catccttcag gttaagctgc
     7561 aggctgctat tctccccaag attacatcgt gacagatcaa gtacacgtaa aacgtcaaac
     7621 cttgaaagag atggcatgac ttcaatagca ggtggaaaga tagtaattga cctcactcgt
     7681 gacatactct tgatatctgt gataggtttg gcttgatgat cttcatttct tttttgaagg
     7741 gacagacggc gacaattacc ctgtgaagac atgctattcc cactgccatc caatagattc
     7801 acaaattttg cttcacgtga caagttgcag ataaggtcca gaaccatatc atgtacacga
     7861 catacatata cctcgtcatt aaaaccatat atgggctgga tcatacttct attaatgagc
     7921 tggttgaagt aatttaatcc gagcaaatac aagctatttc cttggttttc atggtggaca
     7981 aatccttcgg ccacccactt ccatatcagt tcatctctat gaatcttgct atcttctgga
     8041 tatatacata gatacagtag acaagttttc agatgagaag gtagattaga atagctgaaa
     8101 gagagtattc tccgcatctc ctctaaactg ttatcttctg ttagtccaga gccaagggac
     8161 tgaaggagaa tatcccactc acactttggt ttcatcttct ggtcaccggc caaagcacta
     8221 gctatggtaa ttatggctag tggtacccca ccacatttct ttagaatatc tctcgacact
     8281 tgttcaaatt catttagaca tccattctca tctggaaata ttcttttcca gaagagtatt
     8341 ctggagtcat caacagaaag tggttccatt tgataaaccg aatcaccatg tgatgagcaa
     8401 catgaattag agacactgac aatgcgggtt gtggtgatta gccgactgcc tagattattc
     8461 ctattggaga aagcaaagtt gatgccttcc cataattttt catcccatat atcatcaatt
     8521 atgacaagat accttttgtt ctctagaaat tcacgaagtt ttttaataag ctgattggca
     8581 tccagcatcg caagatctga gtgaggattt ccgagatcaa tgaggatatc ccttaaaacc
     8641 ttcttcatgt gagggttctg accgaccgga acaaaagccc gacaatcgaa atcaccttta
     8701 atcttctcgt ataccgctct agcaagagtg gtcttgccca accctccaaa tccaacaatg
     8761 gagaccttct tcagtctctt attagaggca tcatcgccct ccatggaaag caacctcatg
     8821 aggtcttgat ccctctttcc atatatgcca actagctctg tcgcttcagc atacaaagct
     8881 cgaaggcaag ggtcaatagc aattgttctc gtaggatgag gaacaaatac cttgttcctg
     8941 tcacgcctat cagccacctt ttggagttgc tcttggatgt ccttgatcgc gtgagctatc
     9001 ccatgcttat gcttgacttt cttcaacaac tcggtcgtcc tcttcatgaa ccccttaaat
     9061 ttgttgttgt tatcatcaaa ctgaatgcca tcaacctgta cgaggaactt gtcgacgaca
     9121 tcctcaatga cgtaggagag ctctctgact tcatcggccc agagcttgtc ttggctgtcg
     9181 agctgctccc tcggcacctc accaatcttg atgagggcag cgttcatgct ctcaagctcc
     9241 ttcccgaggt cctcaatatt tttcttgaca cccttgtgca gcttgaactc ctccgtgagc
     9301 agctccccca acttgggaat caggttggaa atggcaccgg tgacaatatc catggtgaag
     9361 ggggcggccg cggagcctgc ttttttgtac aaacttgttg atgggctgca ggaattcgat
     9421 atcaagctta tcgataccgt cgacctcgag ggatttgcag aagtaacacc aaacaacagg
     9481 gtgagcatcg acaaaagaaa cagtaccaag caaataaata gcgtatgaag gcagggctaa
     9541 aaaaatccac atatagctgc tgcatatgcc atcatccaag tatatcaaga tcaaaataat
     9601 tataaaacat acttgtttat tataatagat aggtactcaa ggttagagca tatgaataga
     9661 tgctgcatat gccatcatgt atatgcatca gtaaaaccca catcaacatg tatacctatc
     9721 ctagatcgat acttccatcc atcatcttaa actcgtaact atgaagatgt atggcacaca
     9781 catacagatc caaaattaat aaatccacca gttagtttga aacagtattc tactccgatc
     9841 tagaacgaat gaacgaccgc ccaaccacac cacatcatca caaccaagcg aacaaaaagc
     9901 atctctgtat atgcatcagt aaaacccgca tcaacatgta tacctatcct agatcgatat
     9961 ttccatccat catcttcaat tcgtaactat gaatatgtat ggcacacaca tacagatcca
    10021 aaattaataa atccaccagg tagtttgaaa cagaattcta ctccgattta gaacgaccgc
    10081 ccaaccagac cacatcatca caaccaagcc aaaaaaaaaa catgaaaaga tgacccgaca
    10141 aacaagtgca cggcatatat tgaaataaag gaaaagggca aaccaaaccc tatgcaacga
    10201 aacaaaaaaa aatcatga
//
