LOCUS       sequence-266389-        9330 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Second generation lenti expressing Mash1 and Neurogenin for iN 
            transdifferentation to neurons.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9330)
  AUTHORS   Mertens J, Paquola AC, Ku M, Hatch E, Bohnke L, Ladjevardi S, 
            McGrath S, Campbell B, Lee H, Herdy JR, Goncalves JT, Toda T, Kim Y,
            Winkler J, Yao J, Hetzer MW, Gage FH
  TITLE     Directly Reprogrammed Human Neurons Retain Aging-Associated 
            Transcriptomic Signatures and Reveal Age-Related Nucleocytoplasmic 
            Defects.
  JOURNAL   Cell Stem Cell. 2015 Oct 6. pii: S1934-5909(15)00408-7. doi: 
            10.1016/j.stem.2015.09.001.
  PUBMED    26456686
REFERENCE   2  (bases 1 to 9330)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Stem 
            Cell. 2015 Oct 6. pii: S1934-5909(15)00408-7. doi: 
            10.1016/j.stem.2015.09.001."
COMMENT     Sequence Label: pLVX-TREtight-Ngn2:2A:Ascl1-PGK-Puro (XTP-N2A)
FEATURES             Location/Qualifiers
     source          1..9330
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    680..805
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1302..1535
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1720..1764
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2027..2142
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     promoter        2205..2519
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    2213..2231
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2249..2267
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2284..2302
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2320..2338
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2356..2374
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2391..2409
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2427..2445
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     2492..2516
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             3372..3428
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     promoter        4162..4661
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(4197..4220)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     4594..4613
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             4682..5281
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4682..4701)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5178..5198
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    5298..5886
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5351..5371)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5769..5780)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6093..6726
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(6854..6870)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6854..6870)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6867..6889)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6878..6894
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6902..6908,6909..6926,6927..6932))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6947..6968
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7085..7102)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7256..7844)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7336..7355)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8015..8806,8807..8875))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8638..8657
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8876..8980)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    9028..9162
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9065..9084)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9119..9138
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             join(9330,1..633)
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
ORIGIN
        1 ggaagggcta attcactccc aaagaagaca agatatcctt gatctgtgga tctaccacac
       61 acaaggctac ttccctgatt agcagaacta cacaccaggg ccaggggtca gatatccact
      121 gacctttgga tggtgctaca agctagtacc agttgagcca gataaggtag aagaggccaa
      181 taaaggagag aacaccagct tgttacaccc tgtgagcctg catgggatgg atgacccgga
      241 gagagaagtg ttagagtgga ggtttgacag ccgcctagca tttcatcacg tggcccgaga
      301 gctgcatccg gagtacttca agaactgctg atatcgagct tgctacaagg gactttccgc
      361 tggggacttt ccagggaggc gtggcctggg cgggactggg gagtggcgag ccctcagatc
      421 ctgcatataa gcagctgctt tttgcctgta ctgggtctct ctggttagac cagatctgag
      481 cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt
      541 gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca
      601 gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc ccgaacaggg acttgaaagc
      661 gaaagggaaa ccagaggagc tctctcgacg caggactcgg cttgctgaag cgcgcacggc
      721 aagaggcgag gggcggcgac tggtgagtac gccaaaaatt ttgactagcg gaggctagaa
      781 ggagagagat gggtgcgaga gcgtcagtat taagcggggg agaattagat cgcgatggga
      841 aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa ttaaaacata tagtatgggc
      901 aagcagggag ctagaacgat tcgcagttaa tcctggcctg ttagaaacat cagaaggctg
      961 tagacaaata ctgggacagc tacaaccatc ccttcagaca ggatcagaag aacttagatc
     1021 attatataat acagtagcaa ccctctattg tgtgcatcaa aggatagaga taaaagacac
     1081 caaggaagct ttagacaaga tagaggaaga gcaaaacaaa agtaagacca ccgcacagca
     1141 agcggccggc cgctgatctt cagacctgga ggaggagata tgagggacaa ttggagaagt
     1201 gaattatata aatataaagt agtaaaaatt gaaccattag gagtagcacc caccaaggca
     1261 aagagaagag tggtgcagag agaaaaaaga gcagtgggaa taggagcttt gttccttggg
     1321 ttcttgggag cagcaggaag cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc
     1381 agacaattat tgtctggtat agtgcagcag cagaacaatt tgctgagggc tattgaggcg
     1441 caacagcatc tgttgcaact cacagtctgg ggcatcaagc agctccaggc aagaatcctg
     1501 gctgtggaaa gatacctaaa ggatcaacag ctcctgggga tttggggttg ctctggaaaa
     1561 ctcatttgca ccactgctgt gccttggaat gctagttgga gtaataaatc tctggaacag
     1621 atttggaatc acacgacctg gatggagtgg gacagagaaa ttaacaatta cacaagctta
     1681 atacactcct taattgaaga atcgcaaaac cagcaagaaa agaatgaaca agaattattg
     1741 gaattagata aatgggcaag tttgtggaat tggtttaaca taacaaattg gctgtggtat
     1801 ataaaattat tcataatgat agtaggaggc ttggtaggtt taagaatagt ttttgctgta
     1861 ctttctatag tgaatagagt taggcaggga tattcaccat tatcgtttca gacccacctc
     1921 ccaaccccga ggggacccga caggcccgaa ggaatagaag aagaaggtgg agagagagac
     1981 agagacagat ccattcgatt agtgaacgga tctcgacggt atcgccttta aaagaaaagg
     2041 ggggattggg gggtacagtg caggggaaag aatagtagac ataatagcaa cagacataca
     2101 aactaaagaa ttacaaaaac aaattacaaa aattcaaaat tttcgggttt attacaggga
     2161 cagcagagat ccagtttatc gatgaggccc tttcgtcttc actcgagttt actccctatc
     2221 agtgatagag aacgtatgtc gagtttactc cctatcagtg atagagaacg atgtcgagtt
     2281 tactccctat cagtgataga gaacgtatgt cgagtttact ccctatcagt gatagagaac
     2341 gtatgtcgag tttactccct atcagtgata gagaacgtat gtcgagttta tccctatcag
     2401 tgatagagaa cgtatgtcga gtttactccc tatcagtgat agagaacgta tgtcgaggta
     2461 ggcgtgtacg gtgggaggcc tatataagca gagctcgttt agtgaaccgt cagatcgcct
     2521 ggagaaggat ccgcggccgc gccgccacca tgttcgtcaa atccgagacc ttggagttga
     2581 aggaggaaga ggacgtgtta gtgctgctcg gatcggcctc ccccgccttg gcggccctga
     2641 ccccgctgtc atccagcgcc gacgaagaag aggaggagga gccgggcgcg tcaggcgggg
     2701 cgcgtcggca gcgcggggct gaggccgggc agggggcgcg gggcggcgtg gctgcgggtg
     2761 cggagggctg ccggcccgca cggctgctgg gtctggtaca cgattgcaaa cggcgccctt
     2821 cccgggcgcg ggccgtctcc cgaggcgcca agacggccga gacggtgcag cgcatcaaga
     2881 agacccgtag actgaaggcc aacaaccgcg agcgaaaccg catgcacaac ctcaacgcgg
     2941 cactggacgc gctgcgcgag gtgctcccca cgttccccga ggacgccaag ctcaccaaga
     3001 tcgagaccct gcgcttcgcc cacaactaca tctgggcact caccgagacc ctgcgcctgg
     3061 cggatcactg cgggggcggc ggcgggggcc tgccgggggc gctcttctcc gaggcagtgt
     3121 tgctgagccc gggaggcgcc agcgccgccc tgagcagcag cggagacagc ccctcgcccg
     3181 cctccacgtg gagttgcacc aacagccccg cgccgtcctc ctccgtgtcc tccaattcca
     3241 cctcccccta cagctgcact ttatcgcccg ccagcccggc cgggtcagac atggactatt
     3301 ggcagccccc acctcccgac aagcaccgct atgcacctca cctccccata gccagggatt
     3361 gtatccatat ggctaccaac ttctcacttc tcaagcaggc tggtgacgtc gaagagaatc
     3421 ctggcccaat ggaaagctct gccaagatgg agagcggcgg cgccggccag cagccccagc
     3481 cgcagcccca gcagcccttc ctgccgcccg cagcctgttt ctttgccacg gccgcagccg
     3541 cggcggccgc agccgccgca gcggcagcgc agagcgcgca gcagcagcag cagcagcagc
     3601 agcagcagca gcagcaggcg ccgcagctga gaccggcggc cgacggccag ccctcagggg
     3661 gcggtcacaa gtcagcgccc aagcaagtca agcgacagcg ctcgtcttcg cccgaactga
     3721 tgcgctgcaa acgccggctc aacttcagcg gctttggcta cagcctgccg cagcagcagc
     3781 cggccgccgt ggcgcgccgc aacgagcgcg agcgcaaccg cgtcaagttg gtcaacctgg
     3841 gctttgccac ccttcgggag cacgtcccca acggcgcggc caacaagaag atgagtaagg
     3901 tggagacact gcgctcggcg gtcgagtaca tccgcgcgct gcagcagctg ctggacgagc
     3961 atgacgcggt gagcgccgcc ttccaggcag gcgtcctgtc gcccaccatc tcccccaact
     4021 actccaacga cttgaactcc atggccggct cgccggtctc atcctactcg tcggacgagg
     4081 gctcttacga cccgctcagc cccgaggagc aggagcttct cgacttcacc aactggttct
     4141 gatagacgcg tgaattctac cgggtagggg aggcgctttt cccaaggcag tctggagcat
     4201 gcgctttagc agccccgctg ggcacttggc gctacacaag tggcctctgg cctcgcacac
     4261 attccacatc caccggtagg cgccaaccgg ctccgttctt tggtggcccc ttcgcgccac
     4321 cttctactcc tcccctagtc aggaagttcc cccccgcccc gcagctcgcg tcgtgcagga
     4381 cgtgacaaat ggaagtagca cgtctcacta gtctcgtgca gatggacagc accgctgagc
     4441 aatggaagcg ggtaggcctt tggggcagcg gccaatagca gctttgctcc ttcgctttct
     4501 gggctcagag gctgggaagg ggtgggtccg ggggcgggct caggggcggg ctcaggggcg
     4561 gggcgggcgc ccgaaggtcc tccggaggcc cggcattctg cacgcttcaa aagcgcacgt
     4621 ctgccgcgct gttctcctct tcctcatctc cgggcctttc gacctgcagc ccaagcttac
     4681 catgaccgag tacaagccca cggtgcgcct cgccacccgc gacgacgtcc ccagggccgt
     4741 acgcaccctc gccgccgcgt tcgccgacta ccccgccacg cgccacaccg tcgatccgga
     4801 ccgccacatc gagcgggtca ccgagctgca agaactcttc ctcacgcgcg tcgggctcga
     4861 catcggcaag gtgtgggtcg cggacgacgg cgccgcggtg gcggtctgga ccacgccgga
     4921 gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg cgcatggccg agttgagcgg
     4981 ttcccggctg gccgcgcagc aacagatgga aggcctcctg gcgccgcacc ggcccaagga
     5041 gcccgcgtgg ttcctggcca ccgtcggcgt ctcgcccgac caccagggca agggtctggg
     5101 cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc gccggggtgc ccgccttcct
     5161 ggagacctcc gcgccccgca acctcccctt ctacgagcgg ctcggcttca ccgtcaccgc
     5221 cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc ccggtgcctg
     5281 acgggcgcgt ctggaacaat caacctctgg attacaaaat ttgtgaaaga ttgactggta
     5341 ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc
     5401 atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt
     5461 ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg
     5521 ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt
     5581 tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct
     5641 ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg aagctgacgt
     5701 cctttccatg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg tccttctgct
     5761 acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc
     5821 ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct
     5881 ccccgcctgg aattaattct gcagtcgaga cctagaaaaa catggagcaa tcacaagtag
     5941 caatacagca gctaccaatg ctgattgtgc ctggctagaa gcacaagagg aggaggaggt
     6001 gggttttcca gtcacacctc aggtaccttt aagaccaatg acttacaagg cagctgtaga
     6061 tcttagccac tttttaaaag aaaagagggg actggaaggg ctaattcact cccaacgaag
     6121 acaagatatc cttgatctgt ggatctacca cacacaaggc tacttccctg attagcagaa
     6181 ctacacacca gggccagggg tcagatatcc actgaccttt ggatggtgct acaagctagt
     6241 accagttgag ccagataagg tagaagaggc caataaagga gagaacacca gcttgttaca
     6301 ccctgtgagc ctgcatggga tggatgaccc ggagagagaa gtgttagagt ggaggtttga
     6361 cagccgccta gcatttcatc acgtggcccg agagctgcat ccggagtact tcaagaactg
     6421 ctgatatcga gcttgctaca agggactttc cgctggggac tttccaggga ggcgtggcct
     6481 gggcgggact ggggagtggc gagccctcag atcctgcata taagcagctg ctttttgcct
     6541 gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc taactaggga
     6601 acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc
     6661 tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct
     6721 ctagcagtag tagttcatgt catcttatta ttcagtattt ataacttgca aagaaatgaa
     6781 tatcagagag tgagaggcct tgacattgct agcgtttacc gtcgacctct agctagagct
     6841 tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac
     6901 acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac
     6961 tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc
     7021 tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg
     7081 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc
     7141 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt
     7201 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
     7261 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
     7321 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
     7381 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
     7441 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
     7501 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
     7561 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
     7621 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
     7681 acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
     7741 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
     7801 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
     7861 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga
     7921 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa
     7981 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac
     8041 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga
     8101 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc
     8161 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca
     8221 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta
     8281 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg
     8341 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc
     8401 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg
     8461 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt
     8521 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt
     8581 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata
     8641 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc
     8701 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac
     8761 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa
     8821 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct
     8881 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat
     8941 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc
     9001 cacctgacgt cgacggatcg ggagatcaac ttgtttattg cagcttataa tggttacaaa
     9061 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt
     9121 ggtttgtcca aactcatcaa tgtatcttat catgtctgga tcaactggat aactcaagct
     9181 aaccaaaatc atcccaaact tcccacccca taccctatta ccactgccaa ttacctgtgg
     9241 tttcatttac tctaaacctg tgattcctct gaattatttt cattttaaag aaattgtatt
     9301 tgttaaatat gtactacaaa cttagtagtt
//
