LOCUS       sequence-26642-e         708 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 708)
  AUTHORS   Ohta T, Xiong Y
  TITLE     Phosphorylation- and Skp1-independent in vitro ubiquitination of 
            E2F1 by multiple ROC-cullin ligases.
  JOURNAL   Cancer Res. 2001 Feb 15. 61(4):1347-53.
  PUBMED    11245432
REFERENCE   2  (bases 1 to 708)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cancer Res.
            2001 Feb 15. 61(4):1347-53."
COMMENT     Sequence Label: pcDNA3-myc3-bTrCP
FEATURES             Location/Qualifiers
     source          1..708
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     49..68
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        49..67
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             83..112
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             119..148
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             155..184
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 agctctctgg ctaactagag aacccactgc ttactggctt atcgaaatta atacgactca
       61 ctatagggag acccaagcta tggagcaaaa gctcatctca gaggaagatc tggaattaga
      121 gcaaaagctc atctcagagg aagatctgga attagagcaa aagctcatct cagaggaaga
      181 tctggaattg atggacccgg ccgaggcggt gctgcaggag aaagcgctta agtttatgaa
      241 ttcctcagag agagaagact gtaataatgg cgaaccccct aggaagataa taccagagaa
      301 gaattcactt agacagactt acaacagctg tgccaggctt tgcataaacc aagagacagt
      361 atgtctaaca agcactgcta tgaagactga aaattgtgtg gccaaagcca aacttgccaa
      421 tggcacttcc agcatgattg tgcccaagca gcggaaactc tcagcaagct atgagaagga
      481 aaaggagctg tgtgtcaagt attttgagca gtggtcagag tctgatcaag tggaatttgt
      541 agaacacctt atatcccaaa tgtgtcacta ccagcatggg cacatcaact cctacctaaa
      601 acctatgctg cagagggatt tcataactgc actgccagca cggggtctgg accacatcgc
      661 tgagaacatt ctgtcatact tggacgccaa gtcactgtgt gctgctga
//
