LOCUS       sequence-266424-        3911 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses Prochlorococcus marinus MIT9211 Fd 1 fused to mRFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3911)
  AUTHORS   Campbell IJ, Olmos JL Jr, Xu W, Kahanda D, Atkinson JT, Sparks ON, 
            Miller MD, Phillips GN Jr, Bennett GN, Silberg JJ
  TITLE     Prochlorococcus phage ferredoxin: Structural characterization and 
            electron transfer to cyanobacterial sulfite reductases.
  JOURNAL   J Biol Chem. 2020 May 19. pii: RA120.013501. doi: 
            10.1074/jbc.RA120.013501.
  PUBMED    32434930
REFERENCE   2  (bases 1 to 3911)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2020 May 19. pii: RA120.013501. doi: 
            10.1074/jbc.RA120.013501."
COMMENT     Sequence Label: pFd031.RFP
FEATURES             Location/Qualifiers
     source          1..3911
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     70..88
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        join(109..127,128..133,134..150,151..156,157..182)
                     /label=PLtetO-1 promoter
                     /note="modified phage lambda PL promoter with tet operator 
                     sites (Lutz and Bujard, 1997)"
     protein_bind    109..127
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    134..152
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     CDS             541..1215
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /translation="ASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAKL
                     KVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGVV
                     TVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKMR
                     LKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHST
                     GA"
     terminator      1245..1316
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      1332..1359
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     promoter        1411..1445
                     /label=BBa_J23106
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 0.47"
     CDS             1480..2106
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MMSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWH
                     VKNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGT
                     RPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPT
                     TDSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     primer_bind     complement(1556..1575)
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     terminator      2112..2198
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     rep_origin      complement(2362..2950)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2442..2461)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(3038..3132)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(3153..3812)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     3727..3746
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(join(3813..3911,1..4))
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
ORIGIN
        1 atcaacgtct cattttcgcc agatatcgac gtctaagaaa ccattattat catgacatta
       61 acctataaaa ataggcgtat cacgaggccc tttcgtcttc acctcgagtc cctatcagtg
      121 atagagattg acatccctat cagtgataga gatactgagc acatcagcag gacgcactga
      181 cctgcgtctg ttacgggaaa tttaagaatg gcttcgtaca aggtaacttt ggtgaacgcg
      241 aacgagggct taaactctac tattgaagtg cctgatgatc aatatatcct cgacgccgct
      301 gaggaacaag gcattgacct tccttactct tgccgcgcag gcgcatgctc tacttgcgcc
      361 gggaagatca cggcgggttc agtcgaccag tcagatcaaa gctttctcga cgacgatcag
      421 ctcgaggcgg ggttcgtcct tacctgcgtc gcgtatccta caagcgatgt cacgattact
      481 actcatgcgg aggaggagtt atacgggggc tctggaggtt ctggaggttc tggtgggtcc
      541 gcgagtagcg aagacgttat caaagagttc atgcgtttca aagttcgtat ggaaggttcc
      601 gttaacggtc acgagttcga aatcgaaggt gaaggtgaag gtcgtccgta cgaaggtacc
      661 cagaccgcta aactgaaagt taccaaaggt ggtccgctgc cgttcgcttg ggacatcctg
      721 tccccgcagt tccagtacgg ttccaaagct tacgttaaac acccggctga catcccggac
      781 tacctgaaac tgtccttccc ggaaggtttc aaatgggaac gtgttatgaa cttcgaagac
      841 ggtggtgttg ttaccgttac ccaggactcc tccctgcaag acggtgagtt catctacaaa
      901 gttaaactgc gtggtaccaa cttcccgtcc gacggtccgg ttatgcagaa aaaaaccatg
      961 ggttgggaag cttccaccga acgtatgtac ccggaagacg gtgctctgaa aggtgaaatc
     1021 aaaatgcgtc tgaaactgaa agacggtggt cactacgacg ctgaagttaa aaccacctac
     1081 atggctaaaa aaccggttca gctgccgggt gcttacaaaa ccgacatcaa actggacatc
     1141 acctcccaca acgaagacta caccatcgtt gaacagtacg aacgtgctga aggtcgtcac
     1201 tccaccggtg cttaagaatt ctgataataa tctagaccag gcatcaaata aaacgaaagg
     1261 ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctctact
     1321 agagtcacac tggctcacct tcgggtgggc ctttctgcgt ttataggtac ccgtggatcc
     1381 tctagagtcg acctgcaggc atgcaagctt tttacggcta gctcagtcct aggtatagtg
     1441 ctagcccagc cagagaaaca ctctttaaca gggggtagta tgatgtctcg tttagataaa
     1501 agtaaagtga ttaacagcgc attagagctg cttaatgagg tcggaatcga aggtttaaca
     1561 acccgtaaac tcgcccagaa gctaggtgta gagcagccta cattgtattg gcatgtaaaa
     1621 aataagcggg ctttgctcga cgccttagcc attgagatgt tagataggca ccatactcac
     1681 ttttgccctt tagaagggga aagctggcaa gattttttac gtaataacgc taaaagtttt
     1741 agatgtgctt tactaagtca tcgcgatgga gcaaaagtac atttaggtac acggcctaca
     1801 gaaaaacagt atgaaactct cgaaaatcaa ttagcctttt tatgccaaca aggtttttca
     1861 ctagagaatg cattatatgc actcagcgca gtggggcatt ttactttagg ttgcgtattg
     1921 gaagatcaag agcatcaagt cgctaaagaa gaaagggaaa cacctactac tgatagtatg
     1981 ccgccattat tacgacaagc tatcgaatta tttgatcacc aaggtgcaga gccagccttc
     2041 ttattcggcc ttgaattgat catatgcgga ttagaaaaac aacttaaatg tgaaagtggg
     2101 tcttaaggca tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt cgttttatct
     2161 gttgtttgtc ggtgaacgct ctcctgagta ggacaaatcc gccgccctag acctaggcgt
     2221 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc
     2281 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa
     2341 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
     2401 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
     2461 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
     2521 cgcctttctc ccttcgggaa gcgtggcgct ttctcaatgc tcacgctgta ggtatctcag
     2581 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
     2641 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
     2701 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
     2761 agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat ttggtatctg
     2821 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
     2881 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
     2941 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
     3001 ctcacgttaa gggattttgg tcatgactag tgcttggatt ctcaccaata aaaaacgccc
     3061 ggcggcaacc gagcgttctg aacaaatcca gatggagttc tgaggtcatt actggatcta
     3121 tcaacaggag tccaagcgag ctcgatatca aattacgccc cgccctgcca ctcatcgcag
     3181 tactgttgta attcattaag cattctgccg acatggaagc catcacagac ggcatgatga
     3241 acctgaatcg ccagcggcat cagcaccttg tcgccttgcg tataatattt gcccatggtg
     3301 aaaacggggg cgaagaagtt gtccatattg gccacgttta aatcaaaact ggtgaaactc
     3361 acccagggat tggctgagac gaaaaacata ttctcaataa accctttagg gaaataggcc
     3421 aggttttcac cgtaacacgc cacatcttgc gaatatatgt gtagaaactg ccggaaatcg
     3481 tcgtggtatt cactccagag cgatgaaaac gtttcagttt gctcatggaa aacggtgtaa
     3541 caagggtgaa cactatccca tatcaccagc tcaccgtctt tcattgccat acgaaattcc
     3601 ggatgagcat tcatcaggcg ggcaagaatg tgaataaagg ccggataaaa cttgtgctta
     3661 tttttcttta cggtctttaa aaaggccgta atatccagct gaacggtctg gttataggta
     3721 cattgagcaa ctgactgaaa tgcctcaaaa tgttctttac gatgccattg ggatatatca
     3781 acggtggtat atccagtgat ttttttctcc attttagctt ccttagctcc tgaaaatctc
     3841 gataactcaa aaaatacgcc cggtagtgat cttatttcat tatggtgaaa gttggaacct
     3901 cttacgtgcc g
//
