LOCUS       sequence-266442-       10591 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes the C2B domain of synaptotagmin 1 with mutations R398,399Q 
            for viral expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10591)
  AUTHORS   Courtney NA, Bao H, Briguglio JS, Chapman ER
  TITLE     Synaptotagmin 1 clamps synaptic vesicle fusion in mammalian neurons 
            independent of complexin.
  JOURNAL   Nat Commun. 2019 Sep 9;10(1):4076. doi: 10.1038/s41467-019-12015-w.
  PUBMED    31501440
REFERENCE   2  (bases 1 to 10591)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2019-09-9"; volume: "10(1):4076. doi"; pages: " 
            10.1038/s41467-019-12015-w"
COMMENT     Sequence Label: pFSW Syt1-C2B R398,399Q IRES GFP
FEATURES             Location/Qualifiers
     source          1..10591
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(47..66)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        238..617
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        619..817
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     772..792
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             835..1015
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1062..1187
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1680..1913
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2098..2142
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2440..2557
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2602..3048
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
     regulatory      3059..3068
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    3967..4538
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    3988..4540
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4132..4149)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4359..4378
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(4540..4542,4543..4545,4546..5259)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(4540..4542,4543..4545,4546..5256)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4585..4606)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4846..4865)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5193..5214
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    5283..5871
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5336..5356)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5754..5765)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(5874..5890)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5875..5891)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             6396..6576
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(6602..6619)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6608..6832
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6878..7306
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6965..6984)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7175..7196
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7315..7335)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7320..7649
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7500..7635
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7562..7581
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        7697..7744
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             7763..8137
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    8267..8388
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8304..8323)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8358..8377
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8437..8453)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8437..8453)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8450..8472)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8461..8477
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8485..8491,8492..8509,8510..8515))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8530..8551
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8668..8685)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8839..9427)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8919..8938)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9598..10389,10390..10458))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10221..10240
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10459..10563)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
      241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
      841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
     1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
     2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
     2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt
     2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
     2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
     2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
     2581 agatccagtt tggttaatta aagtgcaagt ggttttagga ccaggatgag gcggggtggg
     2641 ggtgcctacc tgacgaccga ccccgaccca ctggacaagc acccaacccc cattccccaa
     2701 attgcgcatc ccctatcaga gagggggagg ggaaacagga tgcggcgagg cgcgtgcgca
     2761 ctgccagctt cagcaccgcg gacagtgcct tcgcccccgc ctggcggcgc gcgccaccgc
     2821 cgcctcagca ctgaaggcgc gctgacgtca ctcgccggtc ccccgtaaac tccccttccc
     2881 ggccaccttg gtcgcgtccg cgccgccgcc ggcccagccg gaccgcacca cgcgaggcgc
     2941 gagatagggg ggcacgggcg cgaccatctg cgctgcggcg ccggcgactc agcgctgcct
     3001 cagtctgcgg tgggcagcgg aggagtcgtg tcgtgcctga gagcgcaggt acggctaggc
     3061 caccatggtg agtgccagtc atcctgaggc cctggccgcc cctgtcacca ctgttgcgac
     3121 ccttgtccca cacaatgcca ctgagccagc cagtcctggg gaagggaagg aagatgcctt
     3181 ttccaagctg aagcagaagt ttatgaatga gctgcataaa attccattgc caccgtgggc
     3241 cttaatagcc atagccatag ttgcggtcct tttagtcgta acctgctgct tttgtgtctg
     3301 taagaaatgt ttgttcaaaa agaaaaacaa gaagaagggg aaggaaaagg gaggaaagaa
     3361 cgccattaac atgaaagacg tgaaagactt agggaagacc atgaaggatc aggcccttaa
     3421 ggatgacgat gctgaaaccg gactgactga tggagaagaa aaggaagagc ccaaggaaga
     3481 ggagaaagct gagaaagaag agcaagagaa actgggtgac atctgcttct ccctccgcta
     3541 cgtccctact gccggcaaac tgactgttgt cattctggaa gccaagaacc tgaagaagat
     3601 ggatgtgggt ggcttatctg atccctacgt gaagattcac ctgatgcaga acggtaagag
     3661 gctgaagaag aaaaagacga cgattaagaa gaacacactc aacccctact acaacgagtc
     3721 cttcagcttt gaagttccgt tcgagcaaat ccagaaagtg caagtggtgg taactgtttt
     3781 ggactatgac aagattggca agaacgacgc catcggcaaa gtcttcgttg gttacaacag
     3841 cactggggcg gagctgcgac actggtcaga catgctggcc aacccccaac agcccatcgc
     3901 acagtggcac actctgcagg tagaggagga ggttgatgcc atgctggctg tcaagaagta
     3961 aaattccccc tctccctccc ccccctaacg ttactggccg aagccgcttg gaataaggcc
     4021 ggtgtgcgtt tgtctatatg ttattttcca ccatattgcc gtcttttggc aatgtgaggg
     4081 cccggaaacc tggccctgtc ttcttgacga gcattcctag gggtctttcc cctctcgcca
     4141 aaggaatgca aggtctgttg aatgtcgtga aggaagcagt tcctctggaa gcttcttgaa
     4201 gacaaacaac gtctgtagcg accctttgca ggcagcggaa ccccccacct ggcgacaggt
     4261 gcctctgcgg ccaaaagcca cgtgtataag atacacctgc aaaggcggca caaccccagt
     4321 gccacgttgt gagttggata gttgtggaaa gagtcaaatg gctctcctca agcgtattca
     4381 acaaggggct gaaggatgcc cagaaggtac cccattgtat gggatctgat ctggggcctc
     4441 ggtacacatg ctttacatgt gtttagtcga ggttaaaaaa acgtctaggc cccccgaacc
     4501 acggggacgt ggttttcctt tgaaaaacac gatgataata tggtgagcaa gggcgaggag
     4561 ctgttcaccg gggtggtgcc catcctggtc gagctggacg gcgacgtaaa cggccacaag
     4621 ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg gcaagctgac cctgaagttc
     4681 atctgcacca ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac cctgacctac
     4741 ggcgtgcagt gcttcagccg ctaccccgac cacatgaagc agcacgactt cttcaagtcc
     4801 gccatgcccg aaggctacgt ccaggagcgc accatcttct tcaaggacga cggcaactac
     4861 aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat cgagctgaag
     4921 ggcatcgact tcaaggagga cggcaacatc ctggggcaca agctggagta caactacaac
     4981 agccacaacg tctatatcat ggccgacaag cagaagaacg gcatcaaggt gaacttcaag
     5041 atccgccaca acatcgagga cggcagcgtg cagctcgccg accactacca gcagaacacc
     5101 cccatcggcg acggccccgt gctgctgccc gacaaccact acctgagcac ccagtccgcc
     5161 ctgagcaaag accccaacga gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc
     5221 gccgggatca ctctcggcat ggacgagctg tacaagtaaa attcgatatc aagcttatcg
     5281 ataatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg
     5341 ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc
     5401 gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt
     5461 tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca
     5521 ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc
     5581 ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc
     5641 tgttgggcac tgacaattcc gtggtgttgt cggggaaatc atcgtccttt ccttggctgc
     5701 tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc
     5761 tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc
     5821 ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg catcgatacc
     5881 gtcgacctcg agacctagaa aaacatggag caatcacaag tagcaataca gcagctacca
     5941 atgctgattg tgcctggcta gaagcacaag aggaggagga ggtgggtttt ccagtcacac
     6001 ctcaggtacc tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa
     6061 aagaaaaggg gggactggaa gggctaattc actcccaacg aagacaagat atccttgatc
     6121 tgtggatcta ccacacacaa ggctacttcc ctgattggca gaactacaca ccagggccag
     6181 ggatcagata tccactgacc tttggatggt gctacaagct agtaccagtt gagcaagaga
     6241 aggtagaaga agccaatgaa ggagagaaca cccgcttgtt acaccctgtg agcctgcatg
     6301 ggatggatga cccggagaga gaagtattag agtggaggtt tgacagccgc ctagcatttc
     6361 atcacatggc ccgagagctg catccggact gtactgggtc tctctggtta gaccagatct
     6421 gagcctggga gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc
     6481 cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc
     6541 tcagaccctt ttagtcagtg tggaaaatct ctagcagggc ccgtttaaac ccgctgatca
     6601 gcctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc
     6661 ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga aattgcatcg
     6721 cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga cagcaagggg
     6781 gaggattggg aagacaatag caggcatgct ggggatgcgg tgggctctat ggcttctgag
     6841 gcggaaagaa ccagctgggg ctctaggggg tatccccacg cgccctgtag cggcgcatta
     6901 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg
     6961 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa
     7021 gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc
     7081 aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt
     7141 cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca
     7201 acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc
     7261 tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattaatt ctgtggaatg
     7321 tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca
     7381 tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa
     7441 gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta actccgccca
     7501 tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt
     7561 ttatttatgc agaggccgag gccgcctctg cctctgagct attccagaag tagtgaggag
     7621 gcttttttgg aggcctaggc ttttgcaaaa agctcccggg agcttgtata tccattttcg
     7681 gatctgatca gcacgtgttg acaattaatc atcggcatag tatatcggca tagtataata
     7741 cgacaaggtg aggaactaaa ccatggccaa gttgaccagt gccgttccgg tgctcaccgc
     7801 gcgcgacgtc gccggagcgg tcgagttctg gaccgaccgg ctcgggttct cccgggactt
     7861 cgtggaggac gacttcgccg gtgtggtccg ggacgacgtg accctgttca tcagcgcggt
     7921 ccaggaccag gtggtgccgg acaacaccct ggcctgggtg tgggtgcgcg gcctggacga
     7981 gctgtacgcc gagtggtcgg aggtcgtgtc cacgaacttc cgggacgcct ccgggccggc
     8041 catgaccgag atcggcgagc agccgtgggg gcgggagttc gccctgcgcg acccggccgg
     8101 caactgcgtg cacttcgtgg ccgaggagca ggactgacac gtgctacgag atttcgattc
     8161 caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg ccggctggat
     8221 gatcctccag cgcggggatc tcatgctgga gttcttcgcc caccccaact tgtttattgc
     8281 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt
     8341 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctgtat
     8401 accgtcgacc tctagctaga gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa
     8461 ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg
     8521 gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca
     8581 gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg
     8641 tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg
     8701 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
     8761 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
     8821 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
     8881 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
     8941 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
     9001 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
     9061 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
     9121 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
     9181 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
     9241 gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc
     9301 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
     9361 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
     9421 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
     9481 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
     9541 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta
     9601 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
     9661 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
     9721 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca
     9781 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
     9841 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt
     9901 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag
     9961 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt
    10021 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat
    10081 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt
    10141 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc
    10201 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat
    10261 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
    10321 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
    10381 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
    10441 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta
    10501 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc
    10561 gcgcacattt ccccgaaaag tgccacctga c
//
