LOCUS       sequence-266447-       11600 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11600)
  AUTHORS   Park BS, Song DH, Kim HM, Choi BS, Lee H, Lee JO
  TITLE     The structural basis of lipopolysaccharide recognition by the 
            TLR4-MD-2 complex.
  JOURNAL   Nature. 2009 Apr 30;458(7242):1191-5. doi: 10.1038/nature07830. Epub
            2009 Mar 1.
  PUBMED    19252480
REFERENCE   2  (bases 1 to 11600)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature07830"; journalName: "Nature"; date: "2009-04-30- 
            30"; volume: "458"; issue: "7242"; pages: "1191-5"
COMMENT     Sequence Label: TLR4
FEATURES             Location/Qualifiers
     source          1..11600
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_recomb     3170..3997
                     /label=baculovirus recombination region (lef2/ORF603)
                     /note="contains ORF603 and part of lef2"
     CDS             3170..3294
                     /codon_start=3
                     /product="baculovirus late expression factor 2"
                     /label=lef2
                     /translation="HLLIKSQDVYKPPNCQKMKTVDKLCPFAGNCKGLNPICNY"
     CDS             complement(3332..3937)
                     /codon_start=1
                     /product="baculovirus ORF603 protein"
                     /label=ORF603
                     /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNGV
                     IELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIHPI
                     KKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKIILSQ
                     INCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR"
     promoter        4001..4092
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     4017..4034
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     sig_peptide     4189..4248
                     /codon_start=1
                     /gene="Autographa californica gp64"
                     /product="signal sequence from the baculovirus envelope 
                     glycoprotein GP64"
                     /label=GP64 signal sequence
                     /translation="MVSAIVLYVLLAAAAHSAFA"
     CDS             6084..6101
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             6102..6119
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     misc_recomb     6608..7313
                     /label=baculovirus recombination region (ORF1629)
                     /note="contains part of ORF1629"
     3'UTR           6696..7068
                     /label=polyhedrin 3' UTR
                     /note="3' UTR from the Autographa californica 
                     nucleopolyhedrovirus polyhedrin gene (Suzuki et al., 2006)"
     CDS             complement(6725..7313)
                     /codon_start=2
                     /product="baculovirus capsid-associated protein"
                     /label=ORF1629
                     /note="required for viral replication"
                     /translation="IPKSSTTNLIADVLADTINRRRVAMAKSSSEATSNDEGWDDDDNR
                     PNKANTPDVKYVQALFNVFTSSQLYTNDSDERNTKAHNILNDVEPLLQNKTQTNIDKAR
                     LLLQDLASFVALSENPLDSPAIGSEKQPLFETNRNLFYKSIEDLIFKFRYKDAENHLIF
                     ALTYHPKDYKFNELLKYVQQLSVNQQRTESSA"
     CDS             complement(7747..7764)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(8981..8997)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8981..8997)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8994..9016)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9005..9021
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9029..9035,9036..9053,9054..9059))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9074..9095
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9212..9229)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9383..9971)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9463..9482)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10142..10933,10934..11002))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10765..10784
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11003..11107)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     11175..11193
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(11231..11253)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     11353..11372
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     11566..11588
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     11580..11597
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     11581..11597
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 aagcttggct cgtaaagcga gttgaaggat catatttagt tgcgtttatg agataagatt
       61 gaaagcacgt gtaaaatgtt tcccgcgcgt tggcacaact atttacaatg cggccaagtt
      121 ataaaagatt ctaatctgat atgttttaaa acacctttgc ggcccgagtt gtttgcgtac
      181 gtgactagcg aagaagatat gtggaccgca gaacagatag taaaacaaaa ccctagtatt
      241 ggagcaataa tcgatttaac caacacgtct aaatattatg atggtgtgca ttttttgcgg
      301 gcgggcctgt tatacaaaaa aattcaagta cctggccaga ctttgccgcc tgaaagcata
      361 gttcaagaat ttattgacac ggtaaaagaa tttacagaaa agtgtcccgg catgttggtg
      421 ggcgtgcact gcacacacgg tattaatcgc accggttaca tggtgtgcag atatttaatg
      481 cacaccctgg gtattgcgcc gcaggaagcc atagatagat tcgaaaaagc cagaggtcac
      541 aaaattgaaa gacaaaatta cgttcaagat ttattaattt aattaatatt atttgcattc
      601 tttaacaaat actttatcct attttcaaat tgttgcgctt cttccagcga accaaaacta
      661 tgcttcgctt gctccgttta gcttgtagcc gatcagtggc gttgttccaa tcgacggtag
      721 gattaggccg gatattctcc accacaatgt tggcaacgtt gatgttacgt ttatgctttt
      781 ggttttccac gtacgtcttt tggccggtaa tagccgtaaa cgtagtgccg tcgcgcgtca
      841 cgcacaacac cggatgtttg cgcttgtccg cggggtattg aaccgcgcga tccgacaaat
      901 ccaccacttt ggcaactaaa tcggtgacct gcgcgtcttt tttctgcatt atttcgtctt
      961 tcttttgcat ggtttcctgg aagccggtgt acatgcggtt tagatcagtc atgacgcgcg
     1021 tgacctgcaa atctttggcc tcgatctgct tgtccttgat ggcaacgatg cgttcaataa
     1081 actcttgttt tttaacaagt tcctcggttt tttgcgccac caccgcttgc agcgcgtttg
     1141 tgtgctcggt gaatgtcgca atcagcttag tcaccaactg tttgctctcc tcctcccgtt
     1201 gtttgatcgc gggatcgtac ttgccggtgc agagcacttg aggaattact tcttctaaaa
     1261 gccattcttg taattctatg gcgtaaggca atttggactt cataatcagc tgaatcacgc
     1321 cggatttagt aatgagcact gtatgcggct gcaaatacag cgggtcgccc cttttcacga
     1381 cgctgttaga ggtagggccc ccattttgga tggtctgctc aaataacgat ttgtatttat
     1441 tgtctacatg aacacgtata gctttatcac aaactgtata ttttaaactg ttagcgacgt
     1501 ccttggccac gaaccggacc tgttggtcgc gctctagcac gtaccgcagg ttgaacgtat
     1561 cttctccaaa tttaaattct ccaattttaa cgcgagccat tttgatacac gtgtgtcgat
     1621 tttgcaacaa ctattgtttt ttaacgcaaa ctaaacttat tgtggtaagc aataattaaa
     1681 tatgggggaa catgcgccgc tacaacactc gtcgttatga acgcagacgg cgccggtctc
     1741 ggcgcaagcg gctaaaacgt gttgcgcgtt caacgcggca aacatcgcaa aagccaatag
     1801 tacagttttg atttgcatat taacggcgat tttttaaatt atcttattta ataaatagtt
     1861 atgacgccta caactccccg cccgcgttga ctcgctgcac ctcgagcagt tcgttgacgc
     1921 cttcctccgt gtggccgaac acgtcgagcg ggtggtcgat gaccagcggc gtgccgcacg
     1981 cgacgcacaa gtatctgtac accgaatgat cgtcgggcga aggcacgtcg gcctccaagt
     2041 ggcaatattg gcaaattcga aaatatatac agttgggttg tttgcgcata tctatcgtgg
     2101 cgttgggcat gtacgtccga acgttgattt gcatgcaagc cgaaattaaa tcattgcgat
     2161 tagtgcgatt aaaacgttgt acatcctcgc ttttaatcat gccgtcgatt aaatcgcgca
     2221 atcgagtcaa gtgatcaaag tgtggaataa tgttttcttt gtattcccga gtcaagcgca
     2281 gcgcgtattt taacaaacta gccatcttgt aagttagttt catttaatgc aactttatcc
     2341 aataatatat tatgtatcgc acgtcaagaa ttaacaatgc gcccgttgtc gcatctcaac
     2401 acgactatga tagagatcaa ataaagcgcg aattaaatag cttgcgacgc aacgtgcacg
     2461 atctgtgcac gcgttccggc acgagctttg attgtaataa gtttttacga agcgatgaca
     2521 tgacccccgt agtgacaacg atcacgccca aaagaactgc cgactacaaa attaccgagt
     2581 atgtcggtga cgttaaaact attaagccat ccaatcgacc gttagtcgaa tcaggaccgc
     2641 tggtgcgaga agccgcgaag tatggcgaat gcatcgtata acgtgtggag tccgctcatt
     2701 agagcgtcat gtttagacaa gaaagctaca tatttaattg atcccgatga ttttattgat
     2761 aaattgaccc taactccata cacggtattc tacaatggcg gggttttggt caaaatttcc
     2821 ggactgcgat tgtacatgct gttaacggct ccgcccacta ttaatgaaat taaaaattcc
     2881 aattttaaaa aacgcagcaa gagaaacatt tgtatgaaag aatgcgtaga aggaaagaaa
     2941 aatgtcgtcg acatgctgaa caacaagatt aatatgcctc cgtgtataaa aaaaatattg
     3001 aacgatttga aagaaaacaa tgtaccgcgc ggcggtatgt acaggaagag gtttatacta
     3061 aactgttaca ttgcaaacgt ggtttcgtgt gccaagtgtg aaaaccgatg tttaatcaag
     3121 gctctgacgc atttctacaa ccacgactcc aagtgtgtgg gtgaagtcat gcatctttta
     3181 atcaaatccc aagatgtgta taaaccacca aactgccaaa aaatgaaaac tgtcgacaag
     3241 ctctgtccgt ttgctggcaa ctgcaagggt ctcaatccta tttgtaatta ttgaataata
     3301 aaacaattat aaatgtcaaa tttgtttttt attaacgata caaaccaaac gcaacaagaa
     3361 catttgtagt attatctata attgaaaacg cgtagttata atcgctgagg taatatttaa
     3421 aatcattttc aaatgattca cagttaattt gcgacaatat aattttattt tcacataaac
     3481 tagacgcctt gtcgtcttct tcttcgtatt ccttctcttt ttcatttttc tcctcataaa
     3541 aattaacata gttattatcg tatccatata tgtatctatc gtatagagta aattttttgt
     3601 tgtcataaat atatatgtct tttttaatgg ggtgtatagt accgctgcgc atagtttttc
     3661 tgtaatttac aacagtgcta ttttctggta gttcttcgga gtgtgttgct ttaattatta
     3721 aatttatata atcaatgaat ttgggatcgt cggttttgta caatatgttg ccggcatagt
     3781 acgcagcttc ttctagttca attacaccat tttttagcag caccggatta acataacttt
     3841 ccaaaatgtt gtacgaaccg ttaaacaaaa acagttcacc tcccttttct atactattgt
     3901 ctgcgagcag ttgtttgttg ttaaaaataa cagccattgt aatgagacgc acaaactaat
     3961 atcacaaact ggaaatgtct atcaatatat agttgctgat atcatggaga taattaaaat
     4021 gataaccatc tcgcaaataa ataagtattt tactgttttc gtaacagttt tgtaataaaa
     4081 aaacctataa atattccgga ttattcatac cgtcccacca tcgggcgcgg atctatgcta
     4141 ctagtaaatc agtcacacca aggcttcaat aaggaacaca caagcaagat ggtaagcgct
     4201 attgttttat atgtgctttt ggcggcggcg gcgcattctg cctttgcggc ggatcccgag
     4261 ccctgcgtgg aggtggttcc taatattact tatcaatgca tggagctgaa tttctacaaa
     4321 atccccgaca acctcccctt ctcaaccaag aacctggacc tgagctttaa tcccctgagg
     4381 catttaggca gctatagctt cttcagtttc ccagaactgc aggtgctgga tttatccagg
     4441 tgtgaaatcc agacaattga agatggggca tatcagagcc taagccacct ctctacctta
     4501 atattgacag gaaaccccat ccagagttta gccctgggag ccttttctgg actatcaagt
     4561 ttacagaagc tggtggctgt ggagacaaat ctagcatctc tagagaactt ccccattgga
     4621 catctcaaaa ctttgaaaga acttaatgtg gctcacaatc ttatccaatc tttcaaatta
     4681 cctgagtatt tttctaatct gaccaatcta gagcacttgg acctttccag caacaagatt
     4741 caaagtattt attgcacaga cttgcgggtt ctacatcaaa tgcccctact caatctctct
     4801 ttagacctgt ccctgaaccc tatgaacttt atccaaccag gtgcatttaa agaaattagg
     4861 cttcataagc tgactttaag aaataatttt gatagtttaa atgtaatgaa aacttgtatt
     4921 caaggtctgg ctggtttaga agtccatcgt ttggttctgg gagaatttag aaatgaagga
     4981 aacttggaaa agtttgacaa atctgctcta gagggcctgt gcaatttgac cattgaagaa
     5041 ttccgattag catacttaga ctactacctc gatgatatta ttgacttatt taattgtttg
     5101 acaaatgttt cttcattttc cctggtgagt gtgactattg aaagggtaaa agacttttct
     5161 tataatttcg gatggcaaca tttagaatta gttaactgta aatttggaca gtttcccaca
     5221 ttgaaactca aatctctcaa aaggcttact ttcacttcca acaaaggtgg gaatgctttt
     5281 tcagaagttg atctaccaag ccttgagttt ctagatctca gtagaaatgg cttgagtttc
     5341 aaaggttgct gttctcaaag tgattttggg acaaccagcc taaagtattt agatctgagc
     5401 ttcaatggtg ttattaccat gagttcaaac ttcttgggct tagaacaact agaacatctg
     5461 gatttccagc attccaattt gaaacaaatg agtgagtttt cagtattcct atcactcaga
     5521 aacctcattt accttgacat ttctcatact cacaccagag ttgctttcaa tggcatcttc
     5581 aatggcttgt ccagtctcga agtcttgaaa atggctggca attctttcca ggaaaacttc
     5641 cttccagata tcttcacaga gctgagaaac ttgaccttcc tggacctctc tcagtgtcaa
     5701 ctggagcagt tgtctccaac agcatttaac tcactctcca gtcttcaggt actaaatatg
     5761 agccacaaca acttcttttc attggatacg tttccttata agtgtctgaa ctccctccag
     5821 gttcttgatt acagtctcaa tcacataatg acttccaaaa aacaggaact acagcatttt
     5881 ccaagtagtc tagctttctt aaatcttact cagaatgact ttgcttgtac ttgtgaacac
     5941 cagagtttcc tgcaatggat caaggaccag aggcagctct tggtggaagt tgaacgaatg
     6001 gaatgtgcga caccttcaga taagcagggc atgcctgtgc tgagtttgaa tatcacctgt
     6061 cagatgaata agtgagcggc cgcctggttc cgcgtggttc gcatcatcat catcatcact
     6121 gaagatctga tcctttcctg ggacccggca agaaccaaaa actcactctc ttcaaggaaa
     6181 tccgtaatgt taaacccgac acgatgaagc ttgtcgttgg atggaaagga aaagagttct
     6241 acagggaaac ttggacccgc ttcatggaag acagcttccc cattgttaac gaccaagaag
     6301 tgatggatgt tttccttgtt gtcaacatgc gtcccactag acccaaccgt tgttacaaat
     6361 tcctggccca acacgctctg cgttgcgacc ccgactatgt acctcatgac gtgattagga
     6421 tcgtcgagcc ttcatgggtg ggcagcaaca acgagtaccg catcagcctg gctaagaagg
     6481 gcggcggctg cccaataatg aaccttcact ctgagtacac caactcgttc gaacagttca
     6541 tcgatcgtgt catctgggag aacttctaca agcccatcgt ttacatcggt accgactctg
     6601 ctgaagagga ggaaattctc cttgaagttt ccctggtgtt caaagtaaag gagtttgcac
     6661 cagacgcacc tctgttcact ggtccggcgt attaaaacac gatacattgt tattagtaca
     6721 tttattaagc gctagattct gtgcgttgtt gatttacaga caattgttgt acgtatttta
     6781 ataattcatt aaatttataa tctttagggt ggtatgttag agcgaaaatc aaatgatttt
     6841 cagcgtcttt atatctgaat ttaaatatta aatcctcaat agatttgtaa aataggtttc
     6901 gattagtttc aaacaagggt tgtttttccg aaccgatggc tggactatct aatggatttt
     6961 cgctcaacgc cacaaaactt gccaaatctt gtagcagcaa tctagctttg tcgatattcg
     7021 tttgtgtttt gttttgtaat aaaggttcga cgtcgttcaa aatattatgc gcttttgtat
     7081 ttctttcatc actgtcgtta gtgtacaatt gactcgacgt aaacacgtta aataaagctt
     7141 ggacatattt aacatcgggc gtgttagctt tattaggccg attatcgtcg tcgtcccaac
     7201 cctcgtcgtt agaagttgct tccgaagacg attttgccat agccacacga cgcctattaa
     7261 ttgtgtcggc taacacgtcc gcgatcaaat ttgtagttga gctttttgga attatttctg
     7321 attgcgggcg tttttgggcg ggtttcaatc taactgtgcc cgattttaat tcagacaaca
     7381 cgttagaaag cgatggtgca ggcggtggta acatttcaga cggcaaatct actaatggcg
     7441 gcggtggtgg agctgatgat aaatctacca tcggtggagg cgcaggcggg gctggcggcg
     7501 gaggcggagg cggaggtggt ggcggtgatg cagacggcgg tttaggctca aatgtctctt
     7561 taggcaacac agtcggcacc tcaactattg tactggtttc gggcgccgtt tttggtttga
     7621 ccggtctgag acgagtgcga tttttttcgt ttctaatagc ttccaacaat tgttgtctgt
     7681 cgtctaaagg tgcagcgggt tgaggttccg tcggcattgg tggagcgggc ggcaattcag
     7741 acatcgatgg tggtggtggt ggtggaggcg ctggaatgtt aggcacggga gaaggtggtg
     7801 gcggcggtgc cgccggtata atttgttctg gtttagtttg ttcgcgcacg attgtgggca
     7861 ccggcgcagg cgccgctggc tgcacaacgg aaggtcgtct gcttcgaggc agcgcttggg
     7921 gtggtggcaa ttcaatatta taattggaat acaaatcgta aaaatctgct ataagcattg
     7981 taatttcgct atcgtttacc gtgccgatat ttaacaaccg ctcaatgtaa gcaattgtat
     8041 tgtaaagaga ttgtctcaag ctcgcacgcc gataacaagc cttttcattt ttactacagc
     8101 attgtagtgg cgagacactt cgctgtcgtc gacgtacatg tatgctttgt tgtcaaaaac
     8161 gtcgttggca agctttaaaa tatttaaaag aacatctctg ttcagcacca ctgtgttgtc
     8221 gtaaatgttg tttttgataa tttgcgcttc cgcagtatcg acacgttcaa aaaattgatg
     8281 cgcatcaatt ttgttgttcc tattattgaa taaataagat tgtacagatt catatctacg
     8341 attcgtcatg gccaccacaa atgctacgct gcaaacgctg gtacaatttt acgaaaactg
     8401 caaaaacgtc aaaactcggt ataaaataat caacgggcgc tttggcaaaa tatctatttt
     8461 atcgcacaag cccactagca aattgtattt gcagaaaaca atttcggcgc acaattttaa
     8521 cgctgacgaa ataaaagttc accagttaat gagcgaccac ccaaatttta taaaaatcta
     8581 ttttaatcac ggttccatca acaaccaagt gatcgtgatg gactacattg actgtcccga
     8641 tttatttgaa acactacaaa ttaaaggcga gctttcgtac caacttgtta gcaatattat
     8701 tagacagctg tgtgaagcgc tcaacgattt gcacaagcac aatttcatac acaacgacat
     8761 aaaactcgaa aatgtcttat atttcgaagc acttgatcgc gtgtatgttt gcgattacgg
     8821 attgtgcaaa cacgaaaact cacttagcgt gcacgacggc acgttggagt attttagtcc
     8881 ggaaaaaatt cgacaccaca actatgcacg ttcgtttgac tggtacgccg tcggcgtgtt
     8941 aacatacaag ttgctaaccg gcggccaatt cgtaatcatg gtcatagctg tttcctgtgt
     9001 gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag
     9061 cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt
     9121 tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag
     9181 gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg
     9241 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat
     9301 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
     9361 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
     9421 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
     9481 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
     9541 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
     9601 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
     9661 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
     9721 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
     9781 cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct
     9841 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
     9901 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
     9961 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
    10021 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
    10081 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca
    10141 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca
    10201 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc
    10261 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa
    10321 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc
    10381 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca
    10441 acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat
    10501 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag
    10561 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac
    10621 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt
    10681 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt
    10741 gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc
    10801 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat
    10861 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca
    10921 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga
    10981 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg
    11041 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg
    11101 ttccgcgcac atttccccga aaagtgccac ctgacgtcta agaaaccatt attatcatga
    11161 cattaaccta taaaaatagg cgtatcacga ggccctttcg tctcgcgcgt ttcggtgatg
    11221 acggtgaaaa cctctgacac atgcagctcc cggagacggt cacagcttgt ctgtaagcgg
    11281 atgccgggag cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggct
    11341 ggcttaacta tgcggcatca gagcagattg tactgagagt gcaccatatg cggtgtgaaa
    11401 taccgcacag atgcgtaagg agaaaatacc gcatcaggcg ccattcgcca ttcaggctgc
    11461 gcaactgttg ggaagggcga tcggtgcggg cctcttcgct attacgccag ctggcgaaag
    11521 ggggatgtgc tgcaaggcga ttaagttggg taacgccagg gttttcccag tcacgacgtt
    11581 gtaaaacgac ggccagtgcc
//
