LOCUS       sequence-26646-e         751 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 751)
  AUTHORS   Verhoef LG, Heinen C, Selivanova A, Halff EF, Salomons FA, Dantuma 
            NP
  TITLE     Minimal length requirement for proteasomal degradation of 
            ubiquitin-dependent substrates.
  JOURNAL   FASEB J. 2009 Jan . 23(1):123-33.
  PUBMED    18796559
REFERENCE   2  (bases 1 to 751)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "FASEB J. 
            2009 Jan . 23(1):123-33."
COMMENT     Sequence Label: mycUBB+1
FEATURES             Location/Qualifiers
     source          1..751
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(78..302)
                     /codon_start=1
                     /product="human ubiquitin"
                     /label=ubiquitin
                     /translation="MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIF
                     AGKQLEDGRTLSDYNIQKESTLHLVLRLRG"
     intron          432..528
                     /label=SV40 intron
                     /note="modified SV40 intron with splice donor and acceptor 
                     sites"
     primer_bind     complement(645..669)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        complement(666..717)
                     /label=CMVd2 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields moderately reduced expression 
                     relative to the full-length promoter"
ORIGIN
        1 cgcggccgct cgagtcactg ggctccactt ccagggtgat ggtcttgccg gtcagggtct
       61 tcacgaaggc tagcataccc tctcagacgc aggaccaggt gcagggtcga ctccttctgg
      121 atgttgtagt cagaaagagt acggccatct tccagctgct tgcctgcaaa gatgagcctc
      181 tgctggtcgg ggggaatgcc ttccttatcc tggatcttgg ccttcacatt ttcgatggtg
      241 tcactgggct ccacctcaag ggtgatggtc ttgccggtaa gggttttcac gaagatctgc
      301 atggcgggaa ttcgggcctc catggccata agcaggtcct cctctgagat cagcttctgc
      361 attgatgcca tggtgggccc gcgggtacaa ttccgcagct tttagagcag aagtaacact
      421 tccgtacagg cctagaagta aaggcaacat ccactgagga gcagttcttt gatttgcacc
      481 accaccggat ccgggacctg aaataaaaga caaaaagact aaacttacca gttaactttc
      541 tggtttttca gttcctctag taccggatcc tctagagtcc ggaggctgga tcggtcccgg
      601 tgtcttctat ggaggtcaaa acagcgtgga tggcgtctcc aggcgatctg acggttcact
      661 aaacgagctc tgcttatata gacctcccac cgtacacgcc taccgcccat ttgcnncaat
      721 ggggcggant tgttacnana ttttggaaag t
//
