LOCUS       sequence-26647-e         781 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 781)
  AUTHORS   Verhoef LG, Heinen C, Selivanova A, Halff EF, Salomons FA, Dantuma 
            NP
  TITLE     Minimal length requirement for proteasomal degradation of 
            ubiquitin-dependent substrates.
  JOURNAL   FASEB J. 2009 Jan . 23(1):123-33.
  PUBMED    18796559
REFERENCE   2  (bases 1 to 781)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "FASEB J. 
            2009 Jan . 23(1):123-33."
COMMENT     Sequence Label: mycUb-20aa
FEATURES             Location/Qualifiers
     source          1..781
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      60..69
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(106..330)
                     /codon_start=1
                     /product="human ubiquitin"
                     /label=ubiquitin
                     /translation="MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIF
                     AGKQLEDGRTLSDYNIQKESTLHLVLRLRG"
     intron          460..556
                     /label=SV40 intron
                     /note="modified SV40 intron with splice donor and acceptor 
                     sites"
     primer_bind     complement(673..697)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        complement(694..745)
                     /label=CMVd2 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields moderately reduced expression 
                     relative to the full-length promoter"
     primer_bind     complement(723..743)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 cggccgcggt acctcgagag anctcggtcg accgaattct cactcctcgc ccttgctcac
       61 catggtggcg accggtggat cctgtcgacc aagcttcccc accacacctc tgagacggag
      121 taccaggtgc agggtggact ctttctggat gttgtagtca gacagggtgc gcccatcttc
      181 cagctgtttt ccagcaaaga tcagcctctg ctggtcagga gggatgcctt ccttatcttg
      241 gatctttgcc ttgacattct caatggtgtc actgggctca acctcgaggg tgatggtctt
      301 accagtcaga gtcttcacga agatctgcat ggtccgaatt cgggcctcca tggccataag
      361 caggtcctcc tctgagatca gcttctgcat tgatgccatg gtgggcccgc gggtacaatt
      421 ccgcagcttt tagagcagaa gtaacacttc cgtacaggcc tagaagtaaa ggcaacatcc
      481 actgaggagc agttctttga tttgcaccac caccggatcc gggacctgaa ataaaagaca
      541 aaaagactaa acttaccagt taactttctg gtttttcagt tcctctagta ccggatcctc
      601 tagagtccgg aggctggatc ggtcccggtg tcttctatgg aggtcaaaac agcgtggatg
      661 gcgtctccag gcgatctgac ggttcactaa acgagctctg cttatataga cctcccaccg
      721 tacacgccta ccgcccattt gcgtcanngg gcgganttgt tacnacnttt tngnaanntc
      781 c
//
