LOCUS       sequence-26648-e         668 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 668)
  AUTHORS   Verhoef LG, Heinen C, Selivanova A, Halff EF, Salomons FA, Dantuma 
            NP
  TITLE     Minimal length requirement for proteasomal degradation of 
            ubiquitin-dependent substrates.
  JOURNAL   FASEB J. 2009 Jan . 23(1):123-33.
  PUBMED    18796559
REFERENCE   2  (bases 1 to 668)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "FASEB J. 
            2009 Jan . 23(1):123-33."
COMMENT     Sequence Label: mycUb-20aa
FEATURES             Location/Qualifiers
     source          1..668
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             20..244
                     /codon_start=1
                     /product="human ubiquitin"
                     /label=ubiquitin
                     /translation="MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIF
                     AGKQLEDGRTLSDYNIQKESTLHLVLRLRG"
     regulatory      281..290
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    354..488
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(378..397)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     432..451
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(599..615)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(599..615)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(612..634)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    623..639
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 ggaggcccga attcgganca tgcagatctt cgtgaagact ctgactggta agaccatcac
       61 cctcgaggtt gagcccagtg acaccattga gaatgtcaag gcaaagatcc aagataagga
      121 aggcatccct cctgaccagc agaggctgat ctttgctgga aaacagctgg aagatgggcg
      181 caccctgtct gactacaaca tccagaaaga gtccaccctg cacctggtac tccgtctcag
      241 aggtgtggtg gggaagcttg gtcgacagga tccaccggtc gccaccatgg tgagcaaggg
      301 cgaggagtga gaattcggtc gaccgagatc tctcgaggta ccgcggccgc ggggatccag
      361 acatgataag atacattgat gagtttggac aaaccacaac tagaatgcag tgaaaaaaat
      421 gctttatttg tgaaatttgt gatgctattg ctttatttgt aaccattata agctgcaata
      481 aacaagttaa caacaacaat tgcattcatt ttatgtttca ggttcagggg gaggtgtggg
      541 aggttttttc ggatcctcta gagtcgatct gcaggcatgc tagcttggcg taatcatggt
      601 catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg
      661 gaagcata
//
