LOCUS       sequence-26649-e         803 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 803)
  AUTHORS   Verhoef LG, Heinen C, Selivanova A, Halff EF, Salomons FA, Dantuma 
            NP
  TITLE     Minimal length requirement for proteasomal degradation of 
            ubiquitin-dependent substrates.
  JOURNAL   FASEB J. 2009 Jan . 23(1):123-33.
  PUBMED    18796559
REFERENCE   2  (bases 1 to 803)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "FASEB J. 
            2009 Jan . 23(1):123-33."
COMMENT     Sequence Label: mycUb-25aa
FEATURES             Location/Qualifiers
     source          1..803
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      75..84
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(121..345)
                     /codon_start=1
                     /product="human ubiquitin"
                     /label=ubiquitin
                     /translation="MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIF
                     AGKQLEDGRTLSDYNIQKESTLHLVLRLRG"
     intron          475..571
                     /label=SV40 intron
                     /note="modified SV40 intron with splice donor and acceptor 
                     sites"
     primer_bind     complement(688..712)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
ORIGIN
        1 cggccgcggt acctcgagag anctcggtcg accgaattct cacaccccgg tgaacagctc
       61 ctcgcccttg ctcaccatgg tggcgaccgg tggatcctgt cgaccaagct tccccaccac
      121 acctctgaga cggagtacca ggtgcagggt ggactctttc tggatgttgt agtcagacag
      181 ggtgcgccca tcttccagct gttttccagc aaagatcagc ctctgctggt caggagggat
      241 gccttcctta tcttggatct ttgccttgac attctcaatg gtgtcactgg gctcaacctc
      301 gagggtgatg gtcttaccag tcagagtctt cacgaagatc tgcatggtcc gaattcgggc
      361 ctccatggcc ataagcaggt cctcctctga gatcagcttc tgcattgatg ccatggtggg
      421 cccgcgggta caattccgca gcttttagag cagaagtaac acttccgtac aggcctagaa
      481 gtaaaggcaa catccactga ggagcagttc tttgatttgc accaccaccg gatccgggac
      541 ctgaaataaa agacaaaaag actaaactta ccagttaact ttctggtttt tcagttcctc
      601 tagtaccgga tcctctagag tccggaggct ggatcggtcc cggtgtcttc tatggaggtc
      661 aaaacagcgt ggatggcgtc tccnggcgat ctgacggttc actaaacgag ctctgcttat
      721 atagacctcc nnnnntacac gccnaccgnc cattngcgtc aatggggncg ganttgttac
      781 nacntttnna ngtcccnnng ant
//
