LOCUS       sequence-266491-        1884 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  BirA*-SIX2 expression. Vector also expresses GFP under control of 
            hUBC promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1884)
  TITLE     Kingsbury Lab Plasmids
REFERENCE   2  (bases 1 to 1884)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pTJK421
FEATURES             Location/Qualifiers
     source          1..1884
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             4..33
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             join(37..384,385..387,388..996)
                     /codon_start=1
                     /product="promiscuous R118G mutant of the E. coli biotin 
                     protein ligase (Cronan, 2005)"
                     /label=BirA(R118G)
                     /note="also known as BirA* or BioID"
                     /translation="KDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRDW
                     GVDVFTVPGKGYSLPEPIQLLNAKQILGQLDGGSVAVLPVIDSTNQYLLDRIGELKSGD
                     ACIAEYQQAGRGGRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEVLRKL
                     GADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESVVNQGW
                     ITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIGD
                     KEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
ORIGIN
        1 atggaacaaa aactcatctc agaagaggat ctcgacaagg acaacaccgt gcccctgaag
       61 ctgatcgccc tgctggccaa cggcgagttc cactctggcg agcagctggg agagaccctg
      121 ggaatgagca gagccgccat caacaagcac atccagacac tgagagactg gggagtggac
      181 gtgttcaccg tgcctggcaa gggctacagc ctgcctgagc ctatccagct gctgaacgcc
      241 aagcagatcc tgggacagct ggatggcgga agcgtggccg tgctgcctgt gatcgactcc
      301 accaatcagt acctgctgga cagaatcgga gagctgaagt ccggcgacgc ctgcatcgcc
      361 gagtaccagc aggctggcag aggaggcaga ggacggaagt ggttcagccc attcggagcc
      421 aacctgtacc tgtccatgtt ctggagactg gagcagggac ctgctgctgc catcggactg
      481 agtctggtga tcggaatcgt gatggccgag gtgctgagaa agctgggagc cgacaaggtg
      541 agagtgaagt ggcctaatga cctgtacctc caggaccgca agctggctgg catcctggtg
      601 gagctgacag gcaagacagg cgatgccgct cagatcgtga tcggagccgg aatcaacatg
      661 gccatgagaa gagtggagga gagcgtggtg aaccagggct ggatcaccct gcaggaggct
      721 ggcatcaacc tggaccggaa caccctggcc gccatgctga tcagagagct gagagccgct
      781 ctggagctgt tcgagcagga gggactggct ccttacctga gcagatggga gaagctggac
      841 aacttcatca acagacctgt gaagctgatc atcggcgaca aggaaatctt cggcatctcc
      901 agaggaatcg acaagcaggg agctctgctg ctggagcagg acggaatcat caagccctgg
      961 atgggcggag aaatctccct gagaagcgca gagaagtcta gaatgtccat gctgcccacc
     1021 ttcggcttca cgcaggagca agtggcgtgc gtgtgcgagg tgctgcagca gggcggcaac
     1081 atcgagcggc tgggccgctt cctgtggtcg ctgcccgcct gcgagcacct tcacaagaat
     1141 gaaagcgtgc tcaaggccaa ggccgtggtg gccttccacc gcggcaactt ccgcgagctc
     1201 tacaagatcc tggagagcca ccagttctcg ccgcacaacc acgccaagct gcagcagctg
     1261 tggctcaagg cacactacat cgaggcggag aagctgcgcg gccgacccct gggcgccgtg
     1321 ggcaaatacc gcgtgcgccg caaattcccg ctgccgcgct ccatctggga cggcgaggag
     1381 accagctact gcttcaagga aaagagtcgc agcgtgctgc gcgagtggta cgcgcacaac
     1441 ccctaccctt caccccgcga gaagcgtgag ctggcggagg ccacgggcct caccaccaca
     1501 caggtcagca actggttcaa gaaccggcgg cagcgcgacc gggcggccga ggccaaggaa
     1561 agggagaaca acgagaactc caattctaac agccacaacc cgctgaatgg cagcggcaag
     1621 tcggtgttag gcagctcgga ggatgagaag actccatcgg ggacgccaga ccactcatca
     1681 tccagccccg cactgctcct cagcccgccg ccccctgggc tgccgtccct gcacagcctg
     1741 ggccaccctc cgggccccag cgcagtgcca gtgccggtgc caggcggagg tggagcggac
     1801 ccactgcaac accaccatgg cctgcaggac tccatcctca accccatgtc agccaacctc
     1861 gtggacctgg gctcctaggt cgac
//
