LOCUS       sequence-266493-        8946 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  lysosomal marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8946)
  AUTHORS   Lim CY, Davis OB, Shin HR, Zhang J, Berdan CA, Jiang X, Counihan JL,
            Ory DS, Nomura DK, Zoncu R
  TITLE     ER-lysosome contacts enable cholesterol sensing by mTORC1 and drive 
            aberrant growth signalling in Niemann-Pick type C.
  JOURNAL   Nat Cell Biol. 2019 Oct;21(10):1206-1218. doi: 
            10.1038/s41556-019-0391-5. Epub 2019 Sep 23.
  PUBMED    31548609
REFERENCE   2  (bases 1 to 8946)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41556-019-0391-5"; journalName: "Nat Cell Biol"; date: 
            "2019-10"; volume: "21"; issue: "10"; pages: "1206-1218"
COMMENT     Sequence Label: pLJM1-FLAG-GFP-Tmem192
FEATURES             Location/Qualifiers
     source          1..8946
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(947..1657,1658..1660))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             complement(join(947..1657,1658..1660))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(989..1010)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     1338..1357
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1597..1618
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     CDS             complement(1676..1699)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     promoter        complement(1743..1946)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(1772..1792)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     complement(2235..2255)
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     CDS             complement(2553..2597)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2782..3015
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    3508..3633
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             3680..3860
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(3861..4087)
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     primer_bind     complement(4115..4135)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(4115..4133)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(4154..4170)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4154..4170)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4167..4189)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4178..4194
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4202..4208,4209..4226,4227..4232))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4247..4268
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4385..4402)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4556..5144)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4636..4655)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5315..6106,6107..6175))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5938..5957
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6176..6280)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(6306..6761)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6443..6464)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     6655..6674
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6888..6910
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     6902..6919
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     6903..6919
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6929..6948
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        6929..6947
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      6968..7103
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    7143..7264
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7154..7173)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     7208..7227
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     LTR             7336..7569
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     CDS             complement(7697..8296)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(7780..7800)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     8277..8296
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     promoter        complement(8318..8828)
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     complement(8381..8399)
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     misc_feature    join(8877..8946,1..48)
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 attctttccc ctgcactgta ccccccaatc cccccttttc ttttaaaatt gtggatgaat
       61 actgccattt gtctcgaggt cgagaattct ctcacgttct acttggctga cagcccaggt
      121 ctgaggaagt gagagccaac aatcgcttac tcagcagcgc attgtgtcgc ttcaggtatt
      181 caatggtgtc tccttgcttt tcaacaattt cttctaggct tgaaatagtt ctgaatccag
      241 tctccgaggt aatattgctg gggtaagcat agattttttc ttcttcaagt atatcaggct
      301 ctggtttagc tttattaaat ctccggattt tcactgtgta aatgaggaga catatcaggg
      361 aacagatgag ttccagtgcc aagatggcca gaatgaggtc aagatacaat ctgccaggct
      421 ctgggaagga gtgctgcatg cacagtatga ggagaagcac tgtgttgcca gaggactgta
      481 tcatcaacgc aagtctcttg agatgccttg ttgatcggta gatcaagtta tagcctcggt
      541 ttctgatttt gctgtggtga tactggatgt agcattcaag gagtaaatgg agaatccaca
      601 aaataacttt cccaaggatt ataaccgtct gaactttcaa tgggtttgtg taatttcctg
      661 ggcacttgtc ctcatttgga ttaggataag aacaaagcac acctgttaaa aatgctaaaa
      721 caacaaacac gagatgaata aaccacagaa gattcactat gatgactgta ggaagaggat
      781 ggaatcgggg tctaaagtga gcttgtaatg agtggtgtgg gagaagctgg gcatccagaa
      841 gtgggtcgtc ttcaatactc tgggtgatat ccaaggaacc gtcctccatc ctgcctccag
      901 ccgccatcgt cgactttgat ccacccccgc ccgatccacc gcctcccttg tacagctcgt
      961 ccatgccgag agtgatcccg gcggcggtca cgaactccag caggaccatg tgatcgcgct
     1021 tctcgttggg gtctttgctc agggcggact gggtgctcag gtagtggttg tcgggcagca
     1081 gcacggggcc gtcgccgatg ggggtgttct gctggtagtg gtcggcgagc tgcacgctgc
     1141 cgtcctcgat gttgtggcgg atcttgaagt tcaccttgat gccgttcttc tgcttgtcgg
     1201 ccatgatata gacgttgtgg ctgttgtagt tgtactccag cttgtgcccc aggatgttgc
     1261 cgtcctcctt gaagtcgatg cccttcagct cgatgcggtt caccagggtg tcgccctcga
     1321 acttcacctc ggcgcgggtc ttgtagttgc cgtcgtcctt gaagaagatg gtgcgctcct
     1381 ggacgtagcc ttcgggcatg gcggacttga agaagtcgtg ctgcttcatg tggtcggggt
     1441 agcggctgaa gcactgcacg ccgtaggtca gggtggtcac gagggtgggc cagggcacgg
     1501 gcagcttgcc ggtggtgcag atgaacttca gggtcagctt gccgtaggtg gcatcgccct
     1561 cgccctcgcc ggacacgctg aacttgtggc cgtttacgtc gccgtccagc tcgaccagga
     1621 tgggcaccac cccggtgaac agctcctcgc ccttgctcac agatccacct ccgcccttgt
     1681 catcgtcatc cttgtagtcc atggcaccgg tagcgctagc ggatctgacg gttcactaaa
     1741 ccagctctgc ttatatagac ctcccaccgt acacgcctac cgcccatttg cgtcaatggg
     1801 gcggagttgt tacgacattt tggaaagtcc cgttgatttt ggtgccaaaa caaactccca
     1861 ttgacgtcaa tggggtggag acttggaaat ccccgtgagt caaaccgcta tccacgccca
     1921 ttgatgtact gccaaaaccg catcaccatg gtaatagcga tgactaatac gtagatgtac
     1981 tgccaagtag gaaagtccca taaggtcatg tactgggcat aatgccaggc gggccattta
     2041 ccgtcattga cgtcaatagg gggcgtactt ggcatatgat agtccatttt aaaacataat
     2101 tttaaaactg caaactaccc aagaaattat tactttctac gtcacgtatt ttgtactaat
     2161 atctttgtgt ttacagtcaa attaattcca attatctctc taacagcctt gtatcgtata
     2221 tgcaaatatg aaggaatcat gggaaatagg ccctcggtga agggggcggc cgctcgaggc
     2281 tagtctcgtg atcgataccg tcgagatccg ttcactaatc gaatggatct gtctctgtct
     2341 ctctctccac cttcttcttc tattccttcg ggcctgtcgg gtcccctcgg ggttgggagg
     2401 tgggtctgaa acgataatgg tgaatatccc tgcctaactc tattcactat agaaagtaca
     2461 gcaaaaacta ttcttaaacc taccaagcct cctactatca ttatgaataa ttttatatac
     2521 cacagccaat ttgttatgtt aaaccaattc cacaaacttg cccatttatc taattccaat
     2581 aattcttgtt cattcttttc ttgctggttt tgcgattctt caattaagga gtgtattaag
     2641 cttgtgtaat tgttaatttc tctgtcccac tccatccagg tcgtgtgatt ccaaatctgt
     2701 tccagagatt tattactcca actagcattc caaggcacag cagtggtgca aatgagtttt
     2761 ccagagcaac cccaaatccc caggagctgt tgatccttta ggtatctttc cacagccagg
     2821 attcttgcct ggagctgctt gatgccccag actgtgagtt gcaacagatg ctgttgcgcc
     2881 tcaatagccc tcagcaaatt gttctgctgc tgcactatac cagacaataa ttgtctggcc
     2941 tgtaccgtca gcgtcattga cgctgcgccc atagtgcttc ctgctgctcc caagaaccca
     3001 aggaacaaag ctcctattcc cactgctctt ttttctctct gcaccactct tctctttgcc
     3061 ttggtgggtg ctactcctaa tggttcaatt tttactactt tatatttata taattcactt
     3121 ctccaattgt ccctcatatc tcctcctcca ggtctgaaga tcagcggccg cttgctgtgc
     3181 ggtggtctta cttttgtttt gctcttcctc tatcttgtct aaagcttcct tggtgtcttt
     3241 tatctctatc ctttgatgca cacaatagag ggttgctact gtattatata atgatctaag
     3301 ttcttctgat cctgtctgaa gggatggttg tagctgtccc agtatttgtc tacagccttc
     3361 tgatgtttct aacaggccag gattaactgc gaatcgttct agctccctgc ttgcccatac
     3421 tatatgtttt aatttatatt ttttctttcc ccctggcctt aaccgaattt tttcccatcg
     3481 cgatctaatt ctcccccgct taatactgac gctctcgcac ccatctctct ccttctagcc
     3541 tccgctagtc aaaatttttg gcgtactcac cagtcgccgc ccctcgcctc ttgccgtgcg
     3601 cgcttcagca agccgagtcc tgcgtcgaga gagctcctct ggtttccctt tcgctttcaa
     3661 gtccctgttc gggcgccact gctagagatt ttccacactg actaaaaggg tctgagggat
     3721 ctctagttac cagagtcaca caacagacgg gcacacacta cttgaagcac tcaaggcaag
     3781 ctttattgag gcttaagcag tgggttccct agttagccag agagctccca ggctcagatc
     3841 tggtctaacc agagagaccc gtttatgtat cgagctaggc acttaaatac aatatctctg
     3901 caatgcggca attcagtggt tcgtccaatc catgtcagac ccgtctgttg ccttcctaat
     3961 aaggcacgat cgtaccacct tacttccacc aatcggcatg cacggtgctt tttctctcct
     4021 tgtaaggcat gttgctaact catcgttacc atgttgcaag actacaagag tattgcataa
     4081 gactacatta agcttgcagc tccagctttt gttcccttta gtgagggtta attgcgcgct
     4141 tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac
     4201 acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac
     4261 tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc
     4321 tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg
     4381 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc
     4441 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt
     4501 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
     4561 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
     4621 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
     4681 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
     4741 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
     4801 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
     4861 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
     4921 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
     4981 acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
     5041 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
     5101 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
     5161 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga
     5221 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa
     5281 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac
     5341 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga
     5401 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc
     5461 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca
     5521 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta
     5581 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg
     5641 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc
     5701 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg
     5761 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt
     5821 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt
     5881 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata
     5941 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc
     6001 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac
     6061 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa
     6121 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct
     6181 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat
     6241 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc
     6301 cacctaaatt gtaagcgtta atattttgtt aaaattcgcg ttaaattttt gttaaatcag
     6361 ctcatttttt aaccaatagg ccgaaatcgg caaaatccct tataaatcaa aagaatagac
     6421 cgagataggg ttgagtgttg ttccagtttg gaacaagagt ccactattaa agaacgtgga
     6481 ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat ggcccactac gtgaaccatc
     6541 accctaatca agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg
     6601 gagcccccga tttagagctt gacggggaaa gccggcgaac gtggcgagaa aggaagggaa
     6661 gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac
     6721 caccacaccc gccgcgctta atgcgccgct acagggcgcg tcccattcgc cattcaggct
     6781 gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa
     6841 agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg
     6901 ttgtaaaacg acggccagtg agcgcgcgta atacgactca ctatagggcg aattgggtac
     6961 gtccctaggc ctccaaaaaa gcctcctcac tacttctgga atagctcaga ggccgaggcg
     7021 gcctcggcct ctgcataaat aaaaaaaatt agtcagccat ggggcggaga atgggcggaa
     7081 ctgggcggag ttaggggcgg gatgggcgga gttaggggcg ggatagctag agccagacat
     7141 gataagatac attgatgagt ttggacaaac cacaactaga atgcagtgaa aaaaatgctt
     7201 tatttgtgaa atttgtgatg ctattgcttt atttgtaacc attataagct gcaataaaca
     7261 agttcctctc actctctgat attcatttct ttgcaagtta taaatactga ataataagat
     7321 gacatgaact actactgcta gagattttcc acactgacta aaagggtctg agggatctct
     7381 agttaccaga gtcacacaac agacgggcac acactacttg aagcactcaa ggcaagcttt
     7441 attgaggctt aagcagtggg ttccctagtt agccagagag ctcccaggct cagatctggt
     7501 ctaaccagag agacccagta caagcaaaaa gcagatcttg tcttcgttgg gagtgaatta
     7561 gcccttccag tccccccttt tcttttaaaa agtggctaag atctacagct gccttgtaag
     7621 tcattggtct taaaggtacc gatgcatggg gtcgtgcgct cctttcggtc gggcgctgcg
     7681 ggtcgtgggg cgggcgtcag gcaccgggct tgcgggtcat gcaccaggtg cgcggtcctt
     7741 cgggcacctc gacgtcggcg gtgacggtga agccgagccg ctcgtagaag gggaggttgc
     7801 ggggcgcgga ggtctccagg aaggcgggca ccccggcgcg ctcggccgcc tccactccgg
     7861 ggagcacgac ggcgctgccc agacccttgc cctggtggtc gggcgagacg ccgacggtgg
     7921 ccaggaacca cgcgggctcc ttgggccggt gcggcgccag gaggccttcc atctgttgct
     7981 gcgcggccag ccgggaaccg ctcaactcgg ccatgcgcgg gccgatctcg gcgaacaccg
     8041 cccccgcttc gacgctctcc ggcgtggtcc agaccgccac cgcggcgccg tcgtccgcga
     8101 cccacacctt gccgatgtcg agcccgacgc gcgtgaggaa gagttcttgc agctcggtga
     8161 cccgctcgat gtggcggtcc ggatcgacgg tgtggcgcgt ggcggggtag tcggcgaacg
     8221 cggcggcgag ggtgcgtacg gccctgggga cgtcgtcgcg ggtggcgagg cgcaccgtgg
     8281 gcttgtactc ggtcatggta agctccggtg gatccccctg gggagagagg tcggtgattc
     8341 ggtcaacgag ggagccgact gccgacgtgc gctccggagg cttgcagaat gcggaacacc
     8401 gcgcgggcag gaacagggcc cacactaccg ccccacaccc cgcctcccgc accgcccctt
     8461 cccggccgct gctctcggcg cgccctgctg agcagccgct attggccaca gcccatcgcg
     8521 gtcggcgcgc tgccattgct ccctggcgct gtccgtctgc gagggtacta gtgagacgtg
     8581 cggcttccgt ttgtcacgtc cggcacgccg cgaaccgcaa ggaaccttcc cgacttaggg
     8641 gcggagcagg aagcgtcgcc ggggggccca caagggtagc ggcgaagatc cgggtgacgc
     8701 tgcgaacgga cgtgaagaat gtgcgagacc cagggtcggc gccgctgcgt ttcccggaac
     8761 cacgcccaga gcagccgcgt ccctgcgcaa acccagggct gccttggaaa aggcgcaacc
     8821 ccaaccccct cgagccgcgg ccaaagtgga tctctgctgt ccctgtaata aacccgaaaa
     8881 ttttgaattt ttgtaatttg tttttgtaat tctttagttt gtatgtctgt tgctattatg
     8941 tctact
//
