LOCUS       sequence-266496-        9621 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  lysosome-targeted Sac1 catalytic domain wild type.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9621)
  AUTHORS   Lim CY, Davis OB, Shin HR, Zhang J, Berdan CA, Jiang X, Counihan JL,
            Ory DS, Nomura DK, Zoncu R
  TITLE     ER-lysosome contacts enable cholesterol sensing by mTORC1 and drive 
            aberrant growth signalling in Niemann-Pick type C.
  JOURNAL   Nat Cell Biol. 2019 Oct;21(10):1206-1218. doi: 
            10.1038/s41556-019-0391-5. Epub 2019 Sep 23.
  PUBMED    31548609
REFERENCE   2  (bases 1 to 9621)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41556-019-0391-5"; journalName: "Nat Cell Biol"; date: 
            "2019-10"; volume: "21"; issue: "10"; pages: "1206-1218"
COMMENT     Sequence Label: pLJM1-Lyso-FLAG-GFP-Sac1-Cat-WT
FEATURES             Location/Qualifiers
     source          1..9621
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    418..651
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             836..880
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     primer_bind     1178..1198
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     promoter        1487..1690
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1641..1661
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             1863..1886
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             join(1902..1904,1905..2615)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             join(1902..1904,1905..2615)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(1944..1965)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2205..2224)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2552..2573
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    4060..4177
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        4226..4736
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     4655..4673
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             4758..5357
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4758..4777)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5254..5274
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             5485..5718
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5790..5911
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5827..5846)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5881..5900
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5951..6086
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6106..6125)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6107..6125)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6135..6152)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6135..6151)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6144..6166)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6293..6748
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6380..6399)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6590..6611
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6774..6878
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6879..6947,6948..7739)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7097..7116)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7910..8498
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8399..8418
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8652..8669
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8786..8807
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8822..8827,8828..8845,8846..8852)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8860..8876
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8865..8887
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8884..8900
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8884..8900
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8919..8939
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        8921..8939
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        8967..9193
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             9194..9374
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    9421..9546
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
ORIGIN
        1 ttaaaacata tagtatgggc aagcagggag ctagaacgat tcgcagttaa tcctggcctg
       61 ttagaaacat cagaaggctg tagacaaata ctgggacagc tacaaccatc ccttcagaca
      121 ggatcagaag aacttagatc attatataat acagtagcaa ccctctattg tgtgcatcaa
      181 aggatagaga taaaagacac caaggaagct ttagacaaga tagaggaaga gcaaaacaaa
      241 agtaagacca ccgcacagca agcggccgct gatcttcaga cctggaggag gagatatgag
      301 ggacaattgg agaagtgaat tatataaata taaagtagta aaaattgaac cattaggagt
      361 agcacccacc aaggcaaaga gaagagtggt gcagagagaa aaaagagcag tgggaatagg
      421 agctttgttc cttgggttct tgggagcagc aggaagcact atgggcgcag cgtcaatgac
      481 gctgacggta caggccagac aattattgtc tggtatagtg cagcagcaga acaatttgct
      541 gagggctatt gaggcgcaac agcatctgtt gcaactcaca gtctggggca tcaagcagct
      601 ccaggcaaga atcctggctg tggaaagata cctaaaggat caacagctcc tggggatttg
      661 gggttgctct ggaaaactca tttgcaccac tgctgtgcct tggaatgcta gttggagtaa
      721 taaatctctg gaacagattt ggaatcacac gacctggatg gagtgggaca gagaaattaa
      781 caattacaca agcttaatac actccttaat tgaagaatcg caaaaccagc aagaaaagaa
      841 tgaacaagaa ttattggaat tagataaatg ggcaagtttg tggaattggt ttaacataac
      901 aaattggctg tggtatataa aattattcat aatgatagta ggaggcttgg taggtttaag
      961 aatagttttt gctgtacttt ctatagtgaa tagagttagg cagggatatt caccattatc
     1021 gtttcagacc cacctcccaa ccccgagggg acccgacagg cccgaaggaa tagaagaaga
     1081 aggtggagag agagacagag acagatccat tcgattagtg aacggatctc gacggtatcg
     1141 atcacgagac tagcctcgag cggccgcccc cttcaccgag ggcctatttc ccatgattcc
     1201 ttcatatttg catatacgat acaaggctgt tagagagata attggaatta atttgactgt
     1261 aaacacaaag atattagtac aaaatacgtg acgtagaaag taataatttc ttgggtagtt
     1321 tgcagtttta aaattatgtt ttaaaatgga ctatcatatg ccaagtacgc cccctattga
     1381 cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt
     1441 tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg
     1501 gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc
     1561 cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg
     1621 taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat
     1681 aagcagagct ggtttagtga accgtcagat ccgctagcgc taccggtgcc atggggtgct
     1741 gctacagcag cgagaacgag gactcggacc aggaccgaga ggagcggaag ctgctgctgg
     1801 accctagcag cccccctacc aaagctctca atggagccga gcccaacggc ggaggtggat
     1861 ctgactacaa ggatgacgat gacaagggcg gaggtggatc tgtgagcaag ggcgaggagc
     1921 tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac ggccacaagt
     1981 tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagctgacc ctgaagttca
     2041 tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc ctgacctacg
     2101 gcgtgcagtg cttcagccgc taccccgacc acatgaagca gcacgacttc ttcaagtccg
     2161 ccatgcccga aggctacgtc caggagcgca ccatcttctt caaggacgac ggcaactaca
     2221 agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc gagctgaagg
     2281 gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac aactacaaca
     2341 gccacaacgt ctatatcatg gccgacaagc agaagaacgg catcaaggtg aacttcaaga
     2401 tccgccacaa catcgaggac ggcagcgtgc agctcgccga ccactaccag cagaacaccc
     2461 ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagcacc cagtccgccc
     2521 tgagcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc gtgaccgccg
     2581 ccgggatcac tctcggcatg gacgagctgt acaagggagg cggtggatcg ggcgggggtg
     2641 gatcaaagtc gacgatgacc aaaaagataa aagtaggtga atttttcagt catgtagtct
     2701 ggaaagcaac agattttgat gtcctttctt ataagaagac aatgttgcac ttaactgata
     2761 ttcagttaca agataataaa accttcctag cgatgctaaa ccatgtcttg aatgtggatg
     2821 gattttactt ttcaacaaca tatgatttga cccatacttt gcagcggcta tccaacacta
     2881 gtcctgaatt ccaagaaatg agtctcttgg aaagggcaga tcagcggttt gtatggaatg
     2941 gtcatcttct aagagaactt tctgcacagc cggaggttca tcggtttgcc cttccagtgt
     3001 tacatggctt tattaccatg cattcatgtt ctattaatgg aaaatacttt gattggattc
     3061 tcatctcgag gaggagctgt ttcagagctg gtgtgcgcta ttatgtaaga ggaattgatt
     3121 cggaaggcca tgcagctaac tttgtagaaa cagaacaaat tgtgcactac aatgggagca
     3181 aagcttcgtt tgtacagact cgaggatcaa tacctgtttt ctggtcccaa agaccaaacc
     3241 tcaagtacaa accactgcca cagatcagca aagtagcaaa tcacatggac ggtttccaaa
     3301 ggcattttga ttcccaagta attatttatg gaaaacaagt tataatcaat ctgattaacc
     3361 agaagggctc ggagaagcca cttgagcaga catttgcaac aatggtgtct tccttgggaa
     3421 gtggaatgat gagatacatt gcctttgact tccataagga atgtaaaaat atgagatggg
     3481 atcgactaag tattttattg gatcaggtag cagaaatgca agatgaatta agttattttc
     3541 tagtggactc tgctggccag gtggtggcaa accaggaagg cgtgttccga agcaattgca
     3601 tggattgtct agatagaacc aatgtgatcc agagtttgtt agctcgtcgt tcacttcagg
     3661 cccaacttca gagactagga gttttgcatg tgggacaaaa gcttgaagaa caagatgaat
     3721 ttgagaagat tttcaaaaat gcctgggctg acaacgcaaa tgcttgtgcc aagcaatatg
     3781 cgggaactgg tgccttgaag actgacttta ccagaactgg aaagagaact catttgggac
     3841 ttataatgga tggctggaac tcaatgatac gatattataa gaacaacttt tccgatggat
     3901 ttagacaaga ttccatagac ttatttcttg gaaactattc agtggatgaa ttagaatctc
     3961 atagtccttt aagtgttcca agggactgga aatgagcggc cgctaagtaa gtaaggatca
     4021 attctcgacc tcgagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
     4081 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
     4141 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
     4201 agatccactt tggccgcggc tcgagggggt tggggttgcg ccttttccaa ggcagccctg
     4261 ggtttgcgca gggacgcggc tgctctgggc gtggttccgg gaaacgcagc ggcgccgacc
     4321 ctgggtctcg cacattcttc acgtccgttc gcagcgtcac ccggatcttc gccgctaccc
     4381 ttgtgggccc cccggcgacg cttcctgctc cgcccctaag tcgggaaggt tccttgcggt
     4441 tcgcggcgtg ccggacgtga caaacggaag ccgcacgtct cactagtacc ctcgcagacg
     4501 gacagcgcca gggagcaatg gcagcgcgcc gaccgcgatg ggctgtggcc aatagcggct
     4561 gctcagcagg gcgcgccgag agcagcggcc gggaaggggc ggtgcgggag gcggggtgtg
     4621 gggcggtagt gtgggccctg ttcctgcccg cgcggtgttc cgcattctgc aagcctccgg
     4681 agcgcacgtc ggcagtcggc tccctcgttg accgaatcac cgacctctct ccccaggggg
     4741 atccaccgga gcttaccatg accgagtaca agcccacggt gcgcctcgcc acccgcgacg
     4801 acgtccccag ggccgtacgc accctcgccg ccgcgttcgc cgactacccc gccacgcgcc
     4861 acaccgtcga tccggaccgc cacatcgagc gggtcaccga gctgcaagaa ctcttcctca
     4921 cgcgcgtcgg gctcgacatc ggcaaggtgt gggtcgcgga cgacggcgcc gcggtggcgg
     4981 tctggaccac gccggagagc gtcgaagcgg gggcggtgtt cgccgagatc ggcccgcgca
     5041 tggccgagtt gagcggttcc cggctggccg cgcagcaaca gatggaaggc ctcctggcgc
     5101 cgcaccggcc caaggagccc gcgtggttcc tggccaccgt cggcgtctcg cccgaccacc
     5161 agggcaaggg tctgggcagc gccgtcgtgc tccccggagt ggaggcggcc gagcgcgccg
     5221 gggtgcccgc cttcctggag acctccgcgc cccgcaacct ccccttctac gagcggctcg
     5281 gcttcaccgt caccgccgac gtcgaggtgc ccgaaggacc gcgcacctgg tgcatgaccc
     5341 gcaagcccgg tgcctgacgc ccgccccacg acccgcagcg cccgaccgaa aggagcgcac
     5401 gaccccatgc atcggtacct ttaagaccaa tgacttacaa ggcagctgta gatcttagcc
     5461 actttttaaa agaaaagggg ggactggaag ggctaattca ctcccaacga agacaagatc
     5521 tgctttttgc ttgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg
     5581 gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag
     5641 tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag
     5701 tgtggaaaat ctctagcagt agtagttcat gtcatcttat tattcagtat ttataacttg
     5761 caaagaaatg aatatcagag agtgagagga acttgtttat tgcagcttat aatggttaca
     5821 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt
     5881 gtggtttgtc caaactcatc aatgtatctt atcatgtctg gctctagcta tcccgcccct
     5941 aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg
     6001 actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa
     6061 gtagtgagga ggcttttttg gaggcctagg gacgtaccca attcgcccta tagtgagtcg
     6121 tattacgcgc gctcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt
     6181 acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag
     6241 gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg ggacgcgccc
     6301 tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt
     6361 gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc
     6421 ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta
     6481 cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc
     6541 tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg
     6601 ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt
     6661 ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat
     6721 tttaacaaaa tattaacgct tacaatttag gtggcacttt tcggggaaat gtgcgcggaa
     6781 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
     6841 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg
     6901 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc
     6961 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg
     7021 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga
     7081 gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc
     7141 aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag
     7201 aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga
     7261 gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg
     7321 cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga
     7381 atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt
     7441 tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact
     7501 ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt
     7561 ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg
     7621 ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta
     7681 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac
     7741 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta
     7801 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
     7861 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     7921 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     7981 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     8041 agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     8101 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
     8161 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     8221 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
     8281 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
     8341 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
     8401 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
     8461 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
     8521 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg
     8581 attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa
     8641 cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc
     8701 ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga
     8761 aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag gcaccccagg
     8821 ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc
     8881 acacaggaaa cagctatgac catgattacg ccaagcgcgc aattaaccct cactaaaggg
     8941 aacaaaagct ggagctgcaa gcttaatgta gtcttatgca atactcttgt agtcttgcaa
     9001 catggtaacg atgagttagc aacatgcctt acaaggagag aaaaagcacc gtgcatgccg
     9061 attggtggaa gtaaggtggt acgatcgtgc cttattagga aggcaacaga cgggtctgac
     9121 atggattgga cgaaccactg aattgccgca ttgcagagat attgtattta agtgcctagc
     9181 tcgatacata aacgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta
     9241 actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg
     9301 tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg
     9361 gaaaatctct agcagtggcg cccgaacagg gacttgaaag cgaaagggaa accagaggag
     9421 ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga
     9481 ctggtgagta cgccaaaaat tttgactagc ggaggctaga aggagagaga tgggtgcgag
     9541 agcgtcagta ttaagcgggg gagaattaga tcgcgatggg aaaaaattcg gttaaggcca
     9601 gggggaaaga aaaaatataa a
//
