LOCUS       sequence-266500-        1296 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lin28-His6 was overexpressed in Escherichia coli Rosetta (DE3) 
            cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1296)
  AUTHORS   O'Day E, Le MT, Imai S, Tan SM, Kirchner R, Arthanari H, Hofmann O, 
            Wagner G, Lieberman J
  TITLE     An RNA-binding Protein, Lin28, Recognizes and Remodels G-quartets in
            the MicroRNAs (miRNAs) and mRNAs It Regulates.
  JOURNAL   J Biol Chem. 2015 Jul 17;290(29):17909-22. doi: 
            10.1074/jbc.M115.665521. Epub 2015 Jun 4.
  PUBMED    26045559
REFERENCE   2  (bases 1 to 1296)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M115.665521"; journalName: "J Biol Chem"; date: 
            "2015-07-17- 17"; volume: "290"; issue: "29"; pages: "17909-22"
COMMENT     Sequence Label: pET15b-mLin28A-cHIS
FEATURES             Location/Qualifiers
     source          1..1296
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     gap             2..11
                     /estimated_length=10
     RBS             33..55
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             524..541
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(600..618)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      614..661
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        complement(join(836..841,842..858,859..864))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     primer_bind     complement(890..908)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 cnnnnnnnnn ngtaggaatt ccctcngaat attttgttta actttaagaa ggagatatac
       61 catggggcag ctgctgcacg gtgcgggcat ctgtaagtgg ttcaacgtgc gcatggggtt
      121 cggcttcctg tccatgaccg cccgcgccgg ggtcgcgctc gaccccccag tggatgtctt
      181 tgtgcaccag agtaagctgc acatggaagg gttccggagc ttgaaggagg gtgaggcagt
      241 ggagttcacc tttaagaagt cagccaaggg tctggaatcc atccgtgtca ccggacctgg
      301 tggagtattc tgtattggga gtgagaggcg gccaaaagga aagagcatgc agaagcgcag
      361 atcaaaagga gacaggtgct acaactgtgg aggtctagat catcatgcca aggaatgcaa
      421 gctgccaccc cagcccaaga agtgccactt ctgccagagc atcagccata tggtagcctc
      481 atgtccgctg aaggcccagc agggccctag tgcacaggga gggcatcatc accatcacca
      541 ctaactcgag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac
      601 cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt
      661 gctgaaagga ggaactatat ccggatatcc cgcaagaggc ccggcagtac cggcataacc
      721 aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta
      781 gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa
      841 agcttatcga tgataagctg tcaaacatga naattcttga aaacgaangg cctcgtgata
      901 cgcctatttt ataggtaant gtcatgataa taatggtttc ttaaacgtca ggtggcactt
      961 ttcgggaaat gtgcgcgnaa ccctatttgt tattttctaa tacattcaat atgtatccnc
     1021 tcatgaaana ataaccctga naatgcttca atanntngaa aangnaaaat atgantntnc
     1081 acntttccng nccccctnat nccntttttg ngnatttnnc ctccnntttn gncccccaaa
     1141 nnctggggna aanaaaaatt tnaaaanant tgggggnccn aagggnttct cnaantgnnt
     1201 nccannngga aaatcnttnn aattttnccc cnaaaaattt tcaaanaaaa anttttaant
     1261 tnntnngggg ggnnttnccg gttnnccnnn aaaaaa
//
